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Quality of Chicken Feather Processed in Different Conditions Rahayu, Sri; Bata, Muhamad
ANIMAL PRODUCTION Vol 16, No 3 (2014): September
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (429.542 KB)

Abstract

Abstract. The objective of this research was to evaluate the hydrolyzed chicken feather based on pepsin digestibility and nutrient content, after physico-chemical and biological process. It was carried out by experimental methods at feed and nutrition laboratory. The treatments were hydrolyzed feather meals immersed in 0.5% NaOH and Na2S solution for 0, 2, 4, 6 and 8 hours, each treatment was repeated three times. The results showed that chemical treatment (NaOH-Na2S) in various time of incubation at 60oC followed by fermentation using Bacillus sp. MTS at 37oC for four days decreased the protein of hydrolyzed feather (78.88 to 73.06%), but increased the keratin fiber (1.9 to 3.26%). Pepsin digestibility informed that the increasing incubation time from 0, 2, 4, 6 to 8 hours resulted in higher solubility than that of control (30.2% at 8 hours vs 15.4% at 0 hours). Processing chicken feather  by  0.5% NaOH and Na2S solution at 60oC for 6 hours followed by fermentation increased the value of pepsin digestibility.  Key words: hydrolyzed, Bacillus sp. MTS, feather, solubility Abstrak. Penelitian ini bertujuan mengevaluasi kualitas nutrien tepung bulu ayam hasil proses hidrolisis secara fisiko-kimia dan biologis menggunakan Bacillus sp. MTS. Metode eksperimental digunakan dalam penelitian yang menggunakan dua tahap proses hidrolisis yaitu tahap 1: setelah perebusan bulu dalam larutan NaOH maka bulu direndam dalam larutan  0.5% NaOH dan Na2S pada 600C dan tahap 2: fermentasi bulu selama empat hari pada suhu 370C. Perlakuan berupa waktu inkubasi yaitu 0, 2, 4, 6 dan 8 jam diterapkan pada tahap kedua dengan ulangan sebanyak tiga kali. Perlakuan fisiko-kimia yang dilanjutkan fermentasi menggunakan bakteri spesifik penghasil enzim-enzim pendegradasi keratin bulu menurunkan kadar  protein tepung bulu  (78,88% menjadi 73,06%) dan meningkatkan kadar serat tepung bulu (1,9 menjadi 3,26%). Uji kelarutan protein tepung bulu dalam pepsin menginfromasikan bahwa proses tahap 1 menghasilkan nilai kelarutan protein tepung bulu yang meningkat dua kali dibanding kontrol (30,2% pada 8 jam vs 15,4% pada 0 jam inkubasi) atau enam kali dibanding tepung bulu tanpa hidrolisis (5%). Pengolahan bulu ayam menggunakan cara pemanasan, perendaman dalam larutan NaOH dan Na2S selama 6 jam pada 600C serta fermentasi menghasilkan tepung bulu dengan daya larut dalam pepsin  lebih baik dibanding tanpa pengolahan.  Kata kunci: hidrolisis, tepung-bulu, Bacillus sp. MTS, kelarutan
Nitrogen Metabolism and Microbial Protein Synthesis by Local Sheep Fed Diet Containing Hibiscus Leave Meal (HLM) with Different Direct-Fed Microbials (DFM) Supplementation Nasution, Risda Amelia Putri; Rahayu, Sri; Bata, Muhamad
ANIMAL PRODUCTION Vol. 22 No. 3 (2020)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with Animal Scientist Society of Indonesia (ISPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (915.095 KB) | DOI: 10.20884/1.jap.2020.22.3.22

Abstract

The interaction of Hibiscus Leaf Meal (HLM) and Direct-Fed Microbials (DFM) supplementation on nitrogen metabolism and rumen microbial synthesis by local sheep was investigated. Thirty six male local sheep aged ±2 years, weight 28.01±2.61 kg were fed concentrate (3% of body weight) supplemented with HLM twice a day, amoniated rice-straw (ARS) supplemented with DFM were given ad-libitum were assigned randomly to nine treatment in an experiment of 3×3 factorial design. The first factor (P) was DFM were supplemented in ARS (P0=without DFM, P1=DFMAMS, and P2=DFMRK). Second factor (W) was the level of HLM supplementation in concentrate (W0=0%, W1=0.24% and W2=0.48% of DM consentrate. The study measured variables including nitrogen digestibility (ND), nitrogen retention (NR), microbial protein synthesis (MPS), and Efficiency of microbial protein synthesis (EMPS). There were significant interaction between DFM and HLM suplementation on MPS and EMPS. However, the interaction of ND and NR was non-existent. Based on polynomial graph, the most efficient MPS was achieved on combination between DFMRK and 0.23% HLM about 8.70 gN/day. Despite the absence of interaction (P>0.05) between DFM supplementation and HLM on KN and RN, DFM supplementation significantly affected (P<0.01) Nitrogen metabolism (ND and NR). The treatment without DFM supplementation resulted the highest nitrogen metabolism (ND and NR). This study concludes that HLM without DFM suplementation positively impacted N Metabolism. Combination between 0.23% HLM and DFMRK resulted the most efficient Microbial Protein Synthesis.
Improving Performance of Heifer Buffalos Fed with Urea-treated Rice Straw Ensiled with Cassava Pulp Supplemented with Concentrates Bata, Muhamad; Sumaryadi, Mas Yedi; Rahayu, Sri; Marung, Nova
ANIMAL PRODUCTION Vol. 22 No. 2 (2020)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with Animal Scientist Society of Indonesia (ISPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jap.2020.22.2.48

Abstract

The objectives of this research were the optimalization of production and reproduction of buffalo heifers through improving feed quality based on rice straw. Twelve heifer buffaloes with body weight of 243 – 350 kg were kept in individual cages and grouped to four. Each groups were randomized to receive three treatments of A, B and C according to Randomized Completely Block Design (RCBD). A was buffalo fed only rice straw. B was buffalo fed rice straw supplemented with concentrate with a dry matter ratio of 70:30. C was similar to B but the rice straw was ammoniated using urea of 4% ensiled with 8% of cassava pulp. In-Vitro test was conducted to measure rumen fermentation product. The results showed the highest of DM and OM digestibility was achieved at C treatment, while the lowest was found at A treatment. Rumen fermentation produsts and its efficiency were the highest in C treatment. In-vivo result showed that ADG of C was higher than that of B and A. Reproductive performance characterized by frequency oestrus for 123 days were an average of 2.5, 2.8 and 4.5 times for A, B and C, respectively. The highest blood metabolite of glucose, urea and erytrocyt was found at C but the leucocyt was lowest compared to A and B. Urea treated rice straw ensiled with cassava pulp and supplemented with concentrate can improve production and reproduction performance of heifer buffaloes.
Feed Intake, Blood Parameters, Digestibility and Live Weight Gain of Male Bali Cattle (Bos javanicus) Fed Ammoniation Rice Straw Supplemented by Waru (Hibiscus tiliaceus) Flower Extracts Rahayu, Sri; Bonat, Veven Raymexen; Bata, Muhamad
ANIMAL PRODUCTION Vol. 23 No. 3 (2021)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with Animal Scientist Society of Indonesia (ISPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jap.2021.23.3.12

Abstract

The objective of this study was to evaluate the effect of Hibiscus tiliaceus flower extracts supplementation in concentrate on performance, nutrient digestibility and blood metabolites of Bali cattle. Sixty of male Bali cattle averaging 225 ± 09.15 kg initial body weight were used in this study. They were divided into two groups. The first group (as control) was cattle fed ammoniated rice straw and concentrate (R1) and the second group was fed as the same as the control group, but concentrate was supplemented with H. tiliaceus flower extract at 200 ppm/kg of dry matter (R2). Variables measured were dry matter digestibility (DMD), organic matter digestibility (OMD), neutral detergent fiber digestibility (NDFD), and acid detergent fiber (ADFD) using total collection method. Other variables were blood glucose and urea, average daily gain (ADG), feed conversion (FC) dan feed efficiency (FE). T-test showed that DMD, OMD, ADFD, and NDFD in the second group (R2) were higher (P<0.05) than control (R1). However, there was no significant difference (P>0.05) between performance (ADG, FC, FE) and blood parameters of urea and glucose between the groups fed diets supplemented with H. tiliaceus flower extracts and control group. There was an increase in ADG and FE on R2 (0.63 ± 0.23 kg and FE 5.54 ± 1.89) compared to R1 (0.54 ± 0.32 kg and 0.54 ± 0.32). The study concluded that supplementation of Hibiscus tiliaceus flower extracts as feed additive in the concentrate diet can increase nutrient digestibility and tend to improve the performance of Bali cattle.
Metabolism Energy and Performance of Several Local Cattle Breeds Fed Rice Straw and Concentrate Efrilia Tri Wahyu Utami; Muhamad Bata; Sri Rahayu
Jurnal Ilmu Ternak dan Veteriner Vol 26, No 2 (2021): June 2021
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v26i2.2711

Abstract

This study was conducted to examine the effect of different local cattle breeds on energy metabolism and performance fed on rice straw basal diet. Fourty local male cattle (2.5 years;  initial BW 300.30±0.68) of Madura cattle (M), Sumba Ongole cattle (SO), Bali cattle (B), and Bali Timor cattle (BT) were used in this study, where types of local breed were used as treatments (10 animals/treatment). The study used a randomized block design (RBD) with cattle's initial body weight as a group. The cattle were fed on rice straw ad libitum and concentrate 2.5% BW (DM 86.53%). The variables measured were energy intake (EI), digestible energy intake (DEI), metaboloizable energy intake (MEI), energy retention (RE), RE to EI ratio, RE to DEI ratio, C2/C3 ratio, the efficiency of hexose conversion to VFA (ECH) and the average daily body weight gain (ADG). The results showed that the different breeds of local cattle had a significant effect (P<0.05) on EI, DEI, MEI, RE, RE to EI ratio, RE to DEI ratio, C2/C3 ratio, and ECH, but had no significant effect on ADG (P>0.05). M has the highest EI, DEI, MEI, and RE 139.52 MJ/day, 99.69 MJ/day, 65.84 MJ/day, and 98.45 MJ/day, respectively, but the highest RE to EI ratio at B, while for the best RE to DE ratio, C2/C3 ratio, ECH, and ADG, at SO were 99.24%, 28.85, 74.97%, and 1.24 kg, respectively. It can be concluded that the best local cattle in terms of performance and feed energy efficiency are Sumba Ongole cattle.
The Use of Various Substrate Microbial Bamboo Stems for Rice Straw Fermentation and its effect on digestibility and fermentation product of Beef Cattle Ration Chabib B.H; Sri Rahayu; Muhamad Bata
Jurnal READ (Research of Empowerment and Development) Vol 1 No 1 (2020): Maret 2020
Publisher : Pascasarjana Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.read.2020.1.1.2386

Abstract

This experiment investigated various substrate growth of bamboo stems microbes (BSM) in rice straw fermentation and its effect on dry matter digestibility (DMD), organic matter digestibility (OMD), NH3-N and VFA concentration. Materials were rumen fluid of beef cattle from the slaughterhouse. Basal diets consisted of rice straw and concentrate with ratio of 40 : 60 (% dry matter) were administered to five treatments namely P0 = untreated rice straw (control), P1 = Ammoniated rice straw, fermented rice straw using microbial bamboo stems grown on ammoniated rice straw (P2), rice bran (P3) and cassava waste (P4). The experiment used in vitro method was designed with Completely Randomized Design and the data were analyzed variance. The results showed that the treatment had no significant effect on dry matter digestibility (DMD), organic matter digestibility (OMD), NH3-N and VFA concentration (P>0,05). The fermentation process of microbial bamboo stems in rice straw with a level of 10% with different substrate did not able to increase the digestibility and products fermentation, but there tends that the rice bran and substrates can increase DMD and N-NH3 concentration
Efektivitas Program Zero Waste Farming untuk Memberdayakan Peternak Domba di Korporasi Dompiland, Kecamatan Pejawaran, Kabupaten Banjarnegara Sugiarto, Mochamad; Bata, Muhamad; Cahyo, Danang Nur; Wakhidati, Yusmi Nur
Jurnal KIRANA Vol. 5 No. 1 (2024): Jurnal Kirana Volume 5 Nomor 1
Publisher : Fakultas Pertanian Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jkrn.v5i1.43999

Abstract

Perusahaan Dompiland Jaya merupakan kelompok ekonomi peternak yang berusaha keras untuk menjadi lebih kuat dan berdaya. Peningkatan pengetahuan dan keterampilan para peternak domba di Dompiland Corporation merupakan aspek penting dan strategis untuk mendorong kelembagaan kelompok ekonomi peternak domba menjadi lebih kuat. Permasalahan yang dihadapi oleh pihak korporasi adalah kurangnya pengetahuan petani mengenai limbah kulit kopi dan keterampilan dalam mengolah pakan silase. Penelitian ini bertujuan untuk mengetahui efektivitas program terhadap peningkatan pengetahuan dan keterampilan peternak mengenai limbah kulit kopi dan pemanfaatannya sebagai bahan pakan konsentrat ternak. Penelitian ini menggunakan 20 responden peternak domba di Perusahaan Dompiland Jaya Kecamatan Pejawaran Kabupaten Banjarnegara. Prosedur pengumpulan data dilakukan melalui pretest dan posttest. Data yang diperoleh dianalisis menggunakan statistik deskriptif dan uji beda sampel berpasangan. Berdasarkan uji berpasangan sampel t-test, pengetahuan petani terhadap limbah kulit kopi dan pemanfaatannya sebagai bahan pakan ternak menunjukkan adanya perbedaan yang nyata sebelum dan sesudah dilakukan kegiatan penyuluhan (P<0,05). Begitu pula dengan keterampilan peternak domba dalam mengolah pakan silase juga meningkat. Program zero waste farming sebagai model pemberdayaan masyarakat efektif meningkatkan pengetahuan dan keterampilan peternak domba di Perusahaan Dompiland Kecamatan Pejawaran Kabupaten Banjarnegara.
PENERAPAN PERTANIAN BERKELANJUTAN MELALUI TEKNOLOGI PENGOLAHAN LIMBAH URINE KAMBING MENJADI BIOURINE PADA KT. LANCAR MAKMUR, KALIBENING BANJARNEGARA Prasetyo, Prasetyo; Cahyo, Danang Nur; Sugiarto, Mochamad; Bata, Muhamad
Journal of Social Outreach Vol 3, No 1 (2024): Journal of Social Outreach
Publisher : Fakultas Sains dan Teknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15548/jso.v3i1.8369

Abstract

Pengabdian kepada masyarakat dilakukan oleh tim dosen Fakultas Peternakan Universitas Jenderal Soedirman di Kecamatan Kalibening Kabupaten Banjarnegara melalui Program Integrasi Peternakan pertanian pada Dataran Tinggi. Kegiatan yang dilakukan yaitu The Development of Integrated Farming System in Upland Areas (UPLAND) Project tahun 2023. Program UPLAND berfokus pada pengolahan limbah peternakan kambing menjadi pupuk organik cair yang dimanfaatkan untuk peningkatan pendapatan petani. Kegiatan ini bertujuan untuk meningkatkan nilai guna dari limbah urine kambing menjadi produk ekonomis tinggi. Kegiatan pengabdian ini dilakukan secara terprogram dimulai dari sosialisasi, praktik pembuatan biourine dan evaluasi kegiatan. Metode yang dilakukan adalah sosialisasi dan demontrasi praktik langsung. Hasil kegiatan pengabdian menunjukan bahwa ketertarikan peserta terhadap inoviasi yang disampaikan sebesar 100% dan sebanyak 76,67% peserta berminat untuk mengadopsi teknologi biourine yang disampaikan dalam program. Peningkatan keterampilan peternak setelah melaksanakan pelatihan ini adalah 46% % meningkat menjadi 78%. Berdasarkan kajian di atas maka disimpulkan bahwa pelatihan pembuatan biourine pada di Kelompok Tani Lancar Makmur diminati oleh peserta dan dapat dipahami oleh anggota kelompok tani dengan indikator minat dan peningkatan pengetahuan yang tinggi. Kata Kunci: Upland, peternakan kambing, biourine, keterampilan peternak
Aktivitas Enzim dan Kecernaan Pakan Domba yang Disuplementasi Complete Rumen Modifier (CRM) Tri Laras Wigati; Fransiska Maria Suhartati; Sri Rahayu; Muhamad Bata
Jurnal Agripet Vol 23, No 1 (2023): Volume 23, No. 1, April 2023
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v23i1.26725

Abstract

ABSTRACT. Penelitian bertujuan mengkaji pengaruh suplementasi complete rumen modifier (CRM) terhadap aktivitas enzim rumen dan kecernaan pakan domba. Delapan belas ekor domba jantan lokal umur 6 8 bulan dengan bobot rata-rata 18,602,11 kg dibagi menjadi 6 kelompok berdasarkan bobot badan. Masing-masing kelompok tersebut diacak untuk mendapatkan satu dari tiga perlakuan sehingga penelitian menggunakan rancangan acak kelompok (RAK). Konsumsi bahan kering yang diberikan adalah 4% dari bobot badan domba dengan imbangan bahan kering jerami padi amoniasi dan konsentrat adalah 60 dan 40%. Perlakuan yang diuji adalah penambahan CRM 0, 1, dan 2% dari bahan kering ransum untuk P1, P2, dan P3. Peubah yang diamati meliputi aktivitas protease, dan selulase rumen, kecernaan bahan kering, protein kasar, dan serat kasar. Hasil penelitian menunjukkan bahwa suplementasi CRM menurunkan aktivitas protease rumen secara kuadrater (P0,01) dengan aktivitas terendah pada taraf CRM 1,16%. Aktivitas selulase meningkat secara kuadrater (P0,01) dengan aktivitas tertinggi pada taraf CRM 1,06% dengan aktivitas enzim sebesar 0,37 U/mg. Suplementasi CRM tidak berpengaruh (P0,05) terhadap kecernaan bahan kering pakan. Kecernaan protein kasar dan serat kasar meningkat secara kuadratik (P0,01) dengan kecernaan tertinggi dicapai pada taraf CRM 1,43% dan 1,69%. Kesimpulan penelitian, suplementasi CRM sebesar 1% - 2% mampu meningkatkan aktivitas enzim rumen dan kecernaan pakan pada domba. Taraf CRM 1% (P2) merupakan taraf terbaik untuk menghasilkan aktivitas enzim rumen dan kecernaan nutrien secara optimal.(Ruminal enzymes activity and nutrient digestibility of sheep supplemented with Complete Rumen Modifier (CRM))ABSTRAK. This research was carried out to study the supplementation of complete rumen modifier (CRM) to improve feed digestibility and rumen enzymes activity in lambs. Eighteen male local lambs age of 6 8 month with average body weight 18.602.11 kg were grouped into 6 blocks by initial body weight. Each group was randomly got one out of three treatments, so the experiment used randomized block design. Dry matter intake (DMI) was 4% of body weight which consisted of concentrate and ammoniated rice straw with ratio 60:40%. As a treatment were the addition of CRM to the concentrate of 0, 1, and 2% based on the diet for P1, P2, and P3 respectively. The variables measured were rumen enzymes activity (protease, and cellulose) and nutrient digestibility (dry matter, crude protein, and crude fiber). The result showed that CRM supplementation quadratically (P0.01) decreased protease activity with the lowest activity at CRM 1,16% but increase cellulose activity quadratically (P0.01) optimum at level CRM 1,06%. CRM supplementation had no effect (P0.05) on dry matter digestibility. CRM supplementation quadratically (P0.01) improved crude protein dan crude fiber digestibility optimum at CRM 1,47% and 1,69% respectively. In conclusion, CRM at a dose 1% - 2% can improve ruminal enzymes and nutrient digestibility in sheep. CRM dose 1% (P2) was the best dose to optimize specific enzymes activity and nutrient digestibility.
Tingkah Laku Makan dan Kecernaan Nutrien Berbagai Bangsa Sapi Lokal yang Diberi Pakan Jerami Padi dan Konsentrat Apit Mulyana; Muhamad Bata; Efka Aris Rimbawanto
Jurnal Agripet Vol 22, No 1 (2022): Volume 22, No. 1, April 2022
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v22i1.20490

Abstract

ABSTRACT. Penelitian ini bertujuan mengetahui pengaruh bangsa sapi yang berbeda terhadap tingkah laku makan dan kecernaan nutrien pada pemberian pakan jerami padi dan konsentrat. Penelitian dilaksanakan selama empat (4) bulan di UD. Sapi Amanah, Desa Karanggintung, Kecamatan Sumbang, Kabupaten Banyumas. Materi yang digunakan adalah 40 ekor sapi lokal jantan dari bangsa sapi Madura (M), Sumba Ongole (SO), Bali (B), dan Bali Timor (BT) masing-masing 10 ekor. Rerata bobot hidup awal masing-masing bangsa sapi berturut-turut adalah 236,904,527 kg, 283,508,873 kg, 224,404,814 kg, dan 282,107,802 kg untuk M, SO, B, dan BT. Sapi tersebut diberi pakan konsentrat 2,5% (BK=86,53%) dari bobot hidup dan jerami padi disediakan secara ad libitum. Penelitian dirancang menurut rancangan acak lengkap dengan empat (4) bangsa sapi sebagai perlakuan. Peubah yang diamati adalah tingkah laku makan dan kecernaan bahan kering (KcBK) dan bahan organik (KcBO). Hasil penelitian menunjukkan bahwa bangsa sapi berpengaruh nyata (P0,05) terhadap kecepatan makan sore hari; lama waktu makan siang, malam dan sehari penuh, KcBK dan KcBO. Akan tetapi, tidak berpengaruh nyata (P0,05) terhadap kecepatan makan pagi hari; frekuensi makan dan ruminasi siang, malam dan sehari penuh; lama waktu ruminasi siang, malam dan sehari penuh. Kesimpulannya adalah terdapat persamaan dan perbedaan tingkah laku makan, kecernaan bahan kering dan bahan organik pada bangsa sapi lokal dan kecernaan BK dan BO pada sapi SO lebih rendah dibandingkan dengan sapi lainnya.(Feeding behavior and nutrient digestibility of various local cattle breeds fed with rice straw and concentrates)ABSTRAK. This study aimed to determine the effect of different breeds of local cattle on feeding behavior and nutrient digestibility of rice straw and concentrate diet. The research was conducted for four (4) months at UD. Sapi Amanah, Karanggintung Village, Sumbang District, Banyumas Regency. The materials used were 40 local bulls from Madura (M), Sumba Ongole (SO), Bali (B), and Bali Timor (BT) and each of the breeds was ten (10) cattle. The initial body weights of each breed were 236,904,527 kg, 283,508,873 kg, 224,404,814 kg and 282,107,802 kg for M, SO, B and BT, respectively. They were fed with 2.5% concentrate (dry matter/DM=86.53%) from body weight and rice straw which was provided ad libitum. The study was designed according to Randomized Complete Design with four (4) local cattle breeds as treatments. Variables measured were feeding behavior and digestibility of DM and organic matter (OM). The results showed that cattle breed had a significant effect (P0.05) on eating rate in the afternoon, duration of daytime, nighttime and one-day meals, DM and OM digestibility. However, there was no significant effect (P0.05) on eating rate in the morning; frequency of daytime, nighttime, and one-day meals and rumination; spent time of daytime, nighttime, and one-day rumination. In conclusion, there are similarities and differences in feeding behavior, DM and OM digestibility among local cattle breeds, and digestibility of dry matter and organic matter of SO was lower than other cattles.