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The Protein-Energy Synchronization Index of The Tropical Legumes for Ruminants Afduha Nurus Syamsi; Merryafinola Ifani; Yusuf Subagyo
Jurnal Peternakan Vol 19, No 1 (2022): Februari 2022
Publisher : State Islamic University of Sultan Syarif Kasim Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24014/jupet.v19i1.15415

Abstract

ABSTRACT. The study was aimed to assess protein-energy synchronization (PES) index of the tropical legumes as feed for ruminants. The research was carried out experimentally in two stages. The first stage was measured the nutritional value of legumes using the proximate method. All types of legumes had been dried (at 600C for 3 days) and grinded. The second stage is to calculate the PES index based on the average degradation of g protein and Kg of organic matter (OM) per hour for each legume. Protein degradation and OM were measured at 2, 4, 6, 8, 12, 24, 48, and 72 hours of observation. The results of the degradation observations at each hour were analyzed by linear regression to obtain the average degradation per hour, then the results were included in the calculation formula of the PES index. The PES index of each legume was discussed descriptively. The analysis showed that legume is a source of fiber with an average crude fiber of 18% and a high protein content of 23.23%. Based on the calculation of the PES index Leucaena leucocephala (0.34) and Calliandra calothyrsus (0.31) are at a low level, while Indigofera (0.48), Sesbania grandiflora (0.42), and Gliricidia sepium (0.47) are at the medium level. This difference was caused by differences in the rate of degradation of protein and organic matter of each legume, besides the presence of antinutrients greatly affected the resulting PES index. The research concluded that the tropical legumes in ruminant feed has the PES indexes at low to medium levels.
Effect of Protection of Soybean Meal Using Mahogany Leaf Extract in Ruminant Diet on Rumen Fermentation Products Merryafinola Ifani; Efka Aris Rimbawanto; Fransisca Maria Suhartati
Jurnal Ilmu Ternak dan Veteriner Vol 26, No 3 (2021): September 2021
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v26i3.2829

Abstract

The study was aimed to examine effect of protecting soybean meal using mahogany leaf extract on rumen fermentation products in vitro. The material used was cow rumen fluid, basal ration consisting of concentrate and elephant grass with a ratio of 60:40%, and mahogany leaves. The research was carried out in three stages: mahogany extraction, protein protection using mahogany extract, and in vitro stages. The test was conducted in vitro based on a completely randomized design (CRD). The treatments consisted of 4 kinds of soybean meal protection with 0% tannin concentration (T0); 1.5% (T1); 3% (T2); and 4.5% (T3). Data obtained were analyzed by analysis of variance and tested using orthogonal polynomials. Results showed that  addition of protected soybean meal with mahogany leaf extract had a cubical effect on partial VFA, methane gas and post-rumen dissolved protein, a quadratic effect on protozoa, and a linear effecton N-NH3, SPM, and RUDP. Giving extra mahogany leaves at a level of 1.5% produced a fermented product that was not different from the control while giving a level of 3% got the highest fermentation product. Giving mahogany leaf extract at a level of 4.5% resulted in the highest SPM, and RUDP but there was a decrease in soluble protein, which indicated the occurrence of overprotection. The addition of 3% mahogany leaf extract effectively increased rumen fermentation products, RUDP, and soluble protein without disturbing the activity of rumen bacteria.
VFA dan N-NH3 Daun Gamal (Gliricidia sepium) pada Ransum Sapi Potong Secara In Vitro Merryafinola Ifani; Efka Aris Rimbawanto; Bambang Hartoyo; Agung Prastyo Nugroho
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 8, No 2 (2021): JITRO, Mei 2021
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (643.177 KB) | DOI: 10.33772/jitro.v8i2.12641

Abstract

ABSTRAKPenelitian ini bertujuan untuk mengetahui pengaruh penggantian konsentrat dengan daun gamal (Gliricidia sepium) terhadap produksi VFA total dan N-NH3 secara in vitro dengan pakan basal jerami padi dengan rasio konsentrat dan jerami padi 60%:40%. Materi yang digunakan dalam percobaan in vitro adalah cairan rumen berasal dari tiga sapi potong di Rumah Potong Hewan Bantarwuni, Kecamatan Kembaran, Kabupaten Banyumas. Ransum yang diuji tersusun dari daun gamal berumur satu tahun dan dipanen umur 60 hari berasal dari Kebumen, jerami padi varietas Umbul-umbul, dan konsentrat. Ransum yang diuji adalah jerami padi dengan konsentrat yang digantikan daun gamal dengan taraf 0; 20; 40; 60% BK. Penelitian menggunakan metode eksperimen secara in vitro yang telah dimodifikasi. Variabel yang diukur adalah produksi VFA total  dan nitrogen amonia (N-NH3­­). Hasil penelitian menunjukan bahwa penambahan daun gamal pada ransum ruminansia berpengaruh sangat nyata (P<0,01) terhadap produksi VFA total  dan N-NH3. Penggantian daun gamal sebanyak 40% BK pada ransum mampu menghasilkan produk VFA dan mengalami penurunan pada taraf penambahan 60% BK, sedangkan penggunaan daun gamal pada taraf 60% BK menghasilkan puncak produksi nitrogen amonia (N-NH3­­).Kata kunci: daun gamal, tanin, VFA total, N-NH3. 
Kecernaan Bahan Kering dan Bahan Organik Leguminosa Pohon Sebagai Sumber Protein Pakan Ruminansia Secara In Vitro Agung Prastyo Nugroho; Efka Aris Rimbawanto; Bambang Hartoyo; Merryafinola Ifani
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 8, No 2 (2021): JITRO, Mei 2021
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (367.245 KB) | DOI: 10.33772/jitro.v8i2.12642

Abstract

ABSTRAK            Penelitian ini bertujuan untuk mengetahui kecernaan bahan kering dan bahan organik dari berbagai macam pemanfaatan leguminosa antara lain: kaliandra, I. zollingeriana, dan lamtoro untuk menggantikan bungkil kedelai dalam ransum ruminansia yang iso-protein secara in vitro. Materi yang digunakan dalam penelitian adalah cairan rumen sapi yang diambil dari Rumah Potong Hewan Bantarwuni Purwokerto. Ransum perlakuan yang digunakan terdiri dari R0 sebagai kontrol dengan sumber protein bungkil kedelai, R1 kaliandra, R2 lamtoro, dan R3 I. zollingeriana. Parameter yang diukur dalam penelitian ini adalah kecernaan bahan kering dan bahan organik. Pengukuran kecernaan dilakukan secara in vitro yang telah dimodifikasi. Hasil analisis variansi menunjukkan bahwa penggunaan berbagai macam leguminosa pohon sebagai sumber protein utama pada pakan ruminansia berpengaruh sangat nyata (p<0,01) antara perlakuan terhadap kecernaan bahan kering dan bahan organik pada pakan ruminansia secara in vitro. Disimpulkan bahwa penggunaan leguminosa pohon sumber protein terbaik untuk menggantikan penggunaan bungkil kedelai yaitu kaliandra.Kata Kunci: Kecernaan in vitro, leguminosa, pakan ruminansia. Tree Legumes Dry Matter and Organic Matter Digestibility as Ruminant Protein Source in VitroABSTRACTThis research was conducted to evaluate dry matter and organic matter digestibility of ruminant feed with different utilizations of legume: Calliandra, I. zollingeriana, and Laucaena leaves to substitute soy bean meal (iso-protein) by in vitro methods. The experiment used rumen fluid collected at Bantarwuni Slaughter House. The treatments are R0 as control (SBM), R1 calliandra, R2 Laucaena, and R3 I. zollingeriana. Variables measured are the digestibility of dry matter and organic matter. The experiment method used to evaluate digestibility was modified in vitro method. Results of variance analysis showed that utilization of different legume as main protein source were significant (p<0,01). The highest digestibility value of dry matter and organic matter reached on R1 treatment (calliandra). It concluded that the best legume to substitute soy bean meal in ruminant feed is Calliandra.Keywords: in vitro digestibility, legume, ruminant feed
Rumen Fermentation Profiles of Protein-Energy Synchronization Index-Based Ration: an In Vitro Study Afduha Nurus Syamsi; Merryafinola Ifani
Jurnal Ilmu Ternak dan Veteriner Vol 28, No 1 (2023): March 2023
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v28i1.3091

Abstract

The study examined the effect of protein-energy synchronization (PES) index-based rations on the rumen fermentation profile. The material used was the Jawa Randu goat's rumen fluid, collected soon after the goat was slaughtered. The treatment ration consisted of elephant grass, lamtoro (Leucaena leucocephala), coconut meal, tofu waste, bran, cassava waste, and mineral mix arranged based on the PES index of each feed ingredient. The research was conducted in vitro using a completely randomized design (CRD). The treatment consisted of 4 PES indexes, namely 0.55 (R1); 0.6 (R2); 0.65 (R3); 0.7 (R4), and each had 5 replications. Data were analyzed by ANOVA and orthogonal polynomials (OP). The results of the ANOVA showed that the PES index did not affect the production of acetate (C2) and butyrate (C4) but had a highly significant effect on dry matter digestibility (DMD), organic matter digestibility (OMD), pH, a total of volatile fatty acids (VFA), propionate (C3), C2:C3 ratio, methane (CH4), ammonia (NH3), and microbial protein synthesis (MPS). The analysis of OP showed a quadratic effect on all variables with the equation Y= - 81.601X2 + 375.04X – 310.78  (DMD), Y= -433.69X2 + 522.69X – 128.75 (OMD), Y=-44X2 + 54.04X – 9.9 (pH), Y= -2160X2 + 2576.8X – 604.2 (VFA Total), Y= -481.8X2 + 585.01X – 143.45 (C3), Y = 50.93X2 – 60.177X + 21.067 (C2:C3), Y = 202.45X2 – 223.18X + 103.41 (CH4), Y= 436X2 – 552.28X + 181.08 (NH3), dan Y= -1012X2 + 1260X – 311.64 (MPS). Protein-energy synchronization (PES) index of 0.6 achieved the best rumen fermentability. The formulation of the PES index-based ration of 0.6 had the most effective compared to other indexes based on the high of propionate, the lowest methane, the lowest NH3, and the best microbial protein synthesis. The ration with an index of 0.6 is composed of 30% elephant grass, 30% Leucaena leucocephala, 10% coconut meal, 10% tofu waste, 10% rice brand, 9% cassava waste, and 1% mineral mix.
Subtitusi Konsentrat dengan Daun Gamal (Gliricidia sepium) Pada Ransum Sapi Potong Secara In Vitro terhadap Kecernaan Bahan Kering dan Organik Merryafinola Ifani; Dwi Wijayanti; Efka Aris Rimbawanto; Bambang Hartoyo
Peternakan Lokal Vol 5 No 1 (2023): Jurnal Peternakan Lokal
Publisher : Program Studi Peternakan Universitas Muslim Maros

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46918/peternakan.v5i1.1721

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh subtitusi konsentrat dengan daun Gamal (Gliricidia sepium) terhadap kecernaan bahan kering dan kecernaan bahan organic secara in vitro dengan pakan basal jerami padi dengan rasio konsentrat dan jerami padi 60:40%. Materi yang digunakan dalam percobaan in vitro adalah cairan rumen berasal dari tiga sapi potong di Rumah Potong Hewan Bantarwuni yang pakan basalnya jerami padi, Kecamatan Kembaran, Kabupaten Banyumas. Daun Gamal berumur satu tahun dan dipanen umur 60 hari berasal dari Kebumen, jerami padi varietas Umbul-umbul dan konsentrat. Perlakuan terdiri dari T0: hijauan 40%+konsentrat 60%, T1: hijauan 40%+konsentrat 40%+ daun Gamal 20%, T2: hijauan 40%+konsentrat 20%+ daun Gamal 40%, T3: hijauan 40%+ daun Gamal 60%. Pengujian dilakukan secara in vitro mengunakan rancangan acak lengkap. Variabel terikat yang diukur adalah kecernaan bahan kering dan kecernaan bahan organik. Hasil penelitian menunjukan bahwa meningkatnya daun Gamal menggantikan konsentrat pada ransum sapi potong dapat meningkatkan (P< 0,05) kecernaan bahan kering dan kecernaan bahan organik. Rata-rata kecernaan bahan kering T0 (72,26%) T1 (79,03%) T2 (85,88%) T3 (86,43%) dan kecernaan bahan organik T0 (80,49%) T1 (85,30%) T2 (87,21%) T3 (94,51%). Hasil uji lanjut Dunnet menunjukan perlakuan T3 merupakan hasil terbaik, maka dapat disimpulkan bahwa daun Gamal dapat menggantikan penggunaan konsentrat sebesar 60%.
KAJIAN METODE TITRASI FORMOL DALAM PENGUKURAN PROTEIN SUSU SEGAR MELALUI VALIDASI METODE ANALISIS (VMA) Hermawan Setyo Widodo; Jodi Susanto; Yusuf Subagyo; Afduha Nurus Syamsi; Merryafinola Ifani
ANGON: Journal of Animal Science and Technology Vol 4 No 3 (2022): JURNAL ANGON
Publisher : Fakultas Peternakan Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (807.28 KB) | DOI: 10.20884/1.angon.2022.4.3.p303-309

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Background. This research was conducted to determine the feasibility of the formol titration method in measuring protein content in fresh milk. Material and method. The material used in this study was a sample of fresh cow's milk. Standard milk was prepared by adding hydrolyzed casein to obtain protein content of 10%, 8%, 6%, 4% and 2%. Each standard was triplicate tested to obtain LOD, LOQ, accuracy and precision calculations. The data obtained is then presented descriptively. Results. The results obtained show that the formol titration method achieves an LOD value of 2.12%, LOQ of 6.41%, an accuracy rate of 145.56% and a precision of 2;4;6;8;10% are 3.58;4.40;3.12;3.58;2.58%. The resulting linear curve has a regression equation Y=2.0228X – 2.785 with an R2 value of 0.957. Conclusion. It can be concluded that the measurement of milk protein using the formol titration method is quite good, but less accurate because it tends to be overquantify.
ANALISIS PERAMALAN PRODUKSI SUSU BERBAGAI TERNAK PERAH DI INDONESIA MENGGUNAKAN METODE AUTOREGRESSIVE INTEGRATED MOVING AVERAGE (ARIMA) Danang Nur Cahyo; Krismiwati Muatip; Afduha Nurus Syamsi; Hermawan Setyo Widodo; Merryafinola Ifani
ANGON: Journal of Animal Science and Technology Vol 4 No 3 (2022): JURNAL ANGON
Publisher : Fakultas Peternakan Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1009.709 KB) | DOI: 10.20884/1.angon.2022.4.3.p310-321

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metabolized by the human body. There are various dairy livestock spread around Indonesia specifically dairy cattle, dairy goats, buffalo, and sheep. This research aimed to forecast the milk production of various dairy livestock in Indonesia until 2028. The research method of this study was the secondary data method utilizing secondary data from the Food and Agriculture Organization (FAO) of the United Nation. Data used in this study was milk production of Indonesia from various dairy livestock in tonnes from 2012-2021. The Autoregressive integrated moving average (ARIMA) method was used to forecast milk production in Indonesia. The (p,d,q) value for the milk production forecast of dairy cattle, dairy goat, buffalo, and sheep were (1,1,1), (2,1,1), (0,0,1), and (1,1,1) respectively. Milk production for cows, goats and sheep is predicted to continue to increase until 2028, while buffalo milk production is predicted to decrease. Milk production from various dairy livestock besides dairy cattle has great potential to develop so as to create diversity in fulfilling the needs of dairy products in Indonesia.
KADAR TOTAL VFA DAN N-NH3 PADA PAKAN RUMINANSIA DENGAN PENGGUNAAN LEGUMINOSA POHON YANG BERBEDA BEBAGAI SUMBER PROTEIN SECARA INVITRO Merryafinola Ifani; Andran Amar Gunawan; Agung Prasetyo Nugroho
ANGON: Journal of Animal Science and Technology Vol 4 No 3 (2022): JURNAL ANGON
Publisher : Fakultas Peternakan Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (798.839 KB) | DOI: 10.20884/1.angon.2022.4.3.p322-327

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Background. The study entitled Total Concentrations of VFA and N-NH3 in Ruminant Feed Using Different Tree Legumes with Various Protein Sources In Vitro, aims to determine the optimal mulberry leaf tannins to be given to livestock in vitro and to determine the effect on total VFA and N-NH3 in vitro. Materials and methods. The material used in the in vitro experiment was rumen fluid from three beef cattle at the Bantarwuni Slaughterhouse, Kembaran District, Banyumas Regency. The feed ingredients used were 40% field grass, 30% cassava, 20% pollar, and 10% from several protein sources (soybean meal (R1), calliandra leaves (R2), lamtoro leaves (R3), and indigofera leaves (R4). The study was conducted in vitro using an experimental method with 5 different treatments, each treatment was repeated 4 times so that there were 20 treatment units.The measured variables were total VFA production and ammonia nitrogen (N-NH3). Results. The results showed that the use of Indigofera leaves in the ration produced the highest total VFA and N-NH3, namely 111.00 ± 20.68 mM and 9.90 ± 0.66 mm. Conclusion. It was concluded that the use of protein source feed in the form of tree legumes can be used as a substitute for soybean meal as feed for ruminants, Indigofera Zollingeriana is a tree legiminosa which is the best substitute for soybean meal.
Pertumbuhan Mikroba dan Derajat Keasaman Susu Sapi Yang Mengalami Pemalsuan Dengan Air Afduha Nurus Syamsi; Merryafinola Ifani; Hermawan Setyo Widodo; Yusuf Subagyo
JAS Vol 8 No 1 (2023): Journal of Animal Science (JAS) - Januari 2023
Publisher : Program Studi Peternakan, Fakultas Pertanian Universitas Timor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (579.245 KB) | DOI: 10.32938/ja.v8i1.3700

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Tujuan penelitian adalah untuk memberikan gambaran potensi percepatan kerusakan susu yang dipalsukan dengan air berdasarkan angka mikroba dan total keasaman. Penelitian dilakukan secara eksperimental dengan materi utama yaitu susu sapi yang diambil segera setelah pemerahan. Rancangan acak lengkap (RAL) faktorial diterapkan dengan faktor A adalah 3 jenis susu (susu tidak dipalsukan (TD), susu dipalsukan dengan air sekitar kandang (DAK), dan susu dipalsukan dengan air rumah (DAR)). Faktor B adalah 6 selang waktu pengamatan (0, 4, 8, 12, 16, dan 20 jam). Penelitian dilakukan dengan 18 kombinasi perlakuan dan masing-masing perlakuan diulang sebanyak 3 kali sehingga terdapat 54 unit percobaan. Peubah Total Plate Count (TPC), pH, serta Soxhlet Henkel (SH) dianalisis variansi dan diuji lanjut dengan orthogonal polynomial, sedangkan indentifikasi kapang dibahas secara deskriptif. Hasil penelitian menunjukkan bahwa interaksi antara jenis pemalsuan susu dan waktu pengamatan berpengaruh sangat nyata (P<0,01) terhadap TPC, pH, dan SH. Analisis regresi menunjukkan bahwa susu TD pada TPC memperoleh persamaan y= 70.246x+137.952 (R2=0.77); DAK yaitu y= 44.925x+203.500 (R2=0.94); dan DAR yaitu y= 67.007x+70.095 (R2=0.72). Susu TD pada pH memperoleh persamaan y=-0.0685x+6.7067 (R2=0.94); DAK yaitu y= -0.097x+6.7767 (R2=0.98); DAR yaitu y= -0.0929x+6.741 (R2=0.96). Susu TD pada 0SH menunjukkan persamaan y= 0.9029x+4.2381 (R2= 0.85); DAK yaitu y= 0.9464x+3.5857 (R2=0.87); DAR yaitu y= 0.9357x+3.4095 (R2=0.88). Identifikasi kapang menunjukkan angka positif pada pengamatan selama 8 dan 12 jam; dan tertinggi ditemukan pada susu DAK. Disimpulkan bahwa susu yang dipalsukan dengan DAK mengalami percepatan pertumbuhan mikroba dan akumulasi asam paling cepat dibandingkan dengan susu yang dipalsukan dengan DAR atau susu TD.