Article Per Year (5 Year)
p-Index From 2021 - 2026
0
P-Index
This Author published in this journals
All Journal Hemera Zoa Jurnal Sain Veteriner Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati
Rini Widayanti
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Veterinary

Published : 6 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 6 Documents
Search

 1 
PCS-13 The Profile and Morphology of Proboscis Monkey’s Blood Cells in South Kalimantan Irma Padeta; Rini Widayanti; Heri Budi Santoso; Amalia Rezeki; Teguh Budipitojo
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (663.074 KB)

Abstract

Twenty percent of primate species is found in Indonesia1. Proboscis monkey (Nasalis larvtaus) or bekantan is one of endemic species in Kalimantan2,3. This primate is belonging to Colobinae subfamily and distributed in Kalimantan (Indonesia), Sabah and Serawak (Malaysia), and Brunei. Proboscis monkey is belonging to endangered species based on International Union for the Conservation of Nature and Natural Resources (IUCN) assessment4. Indonesian Ministry of environment and forestry established Proboscis monkey is belonging to one of twenty five species which has been incrased in population.Blood cells have important role in body regulation such as maintaining body temperature, carriying oxygen, nutrition, hormone and metabolism product to the whole body5. Blood examination is important for body health evaluation6,7, especially in non-human primate where lived in zoo, animal captive or rehabilitiation center6. The aim of this study was to investigate the profile and morphology of female-juvenile proboscis monkey blood cells at rehabilitation center (Sahabat Bekantan Indonesia Foundation) in Banjarmasin, South Kalimantan.
Kajian Penanda Genetik Gen Cytochrome B Pada Tarsius sp. =Study of Genetic Marker on Cytochrome B Gene of Tarsius sp. Rini Widayanti; Dedy Duryadi Solihin; Dondin Sajuthi; RR. Dyah Perwitasari
Jurnal Sain Veteriner Vol 24, No 1 (2006): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1938.625 KB) | DOI: 10.22146/jsv.349

Abstract

Tarsius merupakan salah satu satwa endemik Indonesia yang keberadaannya mulai memprihatinkan. Konservasi sebagai salah satu cara untuk pelestarian satwa ini akan lebih terarah dan berhasil guna apabila karakteristik dan keragaman sumber genetiknya diketahui dengan pasti. Tujuan dari penelitian ini adalah mengkaji penanda genetik spesifik gen cyt b pada Tarsius sp. Pengurutan hasil PCR menggunakan primer H 15149 pada gen cyt b didapatkan urutan basa sebesar 276 pb (menyandi 92 asam amino. Fragmen cyt b hash! pengurutan disejajarkan berganda dengan primata lain dari data Genbank dengan bantuan perangkat lunak Genetyx-Win versi 3.0 dan Clustal W, kemudian dianalisis dengan menggunakan program MEGA versi 3.1. Dari hasil analisis diperoleh 14 situs asam amino yang berbeda. Tarsius dianae memiliki 12 situs asam amino (asam amino ke 2, 6, 9, 22, 23, 29, 39, 41, 42, 45, 55 dan 85), T. spectrum memiliki 7 situs asam amino (asam amino ke 2, 6, 9, 41, 45, 55 dan 85) dan T bancanus memiliki 2 situs asam amino ( ke 23 dan 45) yang dapat digunakan sebagai penanda genetik. Lima asam amino unik ditemukan pada T dianae, yaitu pada situs asam amino ke 6 (valina), ke 22 (alanina), ke 29 (alanina), ke 39 (serina) dan ke 42 (valina). Jarak genetik berdasar nukleotida cyt b yang dihitung menggunakan model 2 parameter Kimura ditemukan nilai paling kecil sebesar 0,7%, nilai paling besar 22,3% dan rata-rata 13,1%. Filogram menggunakan metode neighbor joining berdasar hasil urutan nukleotida dan asam amino cyt b tersebut dapat dijadikan pembeda masing-masing spesies Tarsius.
KARAKTERISASI ANTIBODI MONOKLONAL TERHADAP PROTEIN MEMBRAN Toxoplasma gondii ISOLAT LOKAL Rini Widayanti; Widya Asmara; Wayan Tunas Artama
Jurnal Sain Veteriner Vol 22, No 1 (2004): Juli
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1255.568 KB) | DOI: 10.22146/jsv.443

Abstract

.
Kajian Molekuler Daerah D-Loop Parsial Deoxyribonucleic acid (DNA) Mitokondria Kuda (Equus caballus) Asli Priangan Yuriadi .; Rini Widayanti; Aris Haryanto; Wayan Tunas Artama
Jurnal Sain Veteriner Vol 27, No 2 (2009): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1948.518 KB) | DOI: 10.22146/jsv.463

Abstract

.
Kajian Molekular Tarsius sp. Pada Gen Penyandi Cytochrome Oxidase Sub-unit 2 Mitokondria Rini Widayanti; Niken Satuti Handayani; I. Made Budiarsa
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 1 (2010): February 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i1.2653

Abstract

Tarsius is an endemic species in Indonesia that is endangered. In-situ and ex-situ conservation of this species would yield better results if this genetic make up and diversity is determined. The objective of this ressearch was to study the specific genetic marker on COX2 gene of Tarsius sp. Sequencing of PCR product using primer COX2F and COX2R resulted in base sequence of 513 nts. Results of COX2 fragments sequencing were put on multiple alignment with other primates from Genbank with aid of software Clustal W, and were analyzed using MEGA program version 4.1. Eight different amino acid sites were found (amino acid no. 5, 6, 13, 14, 30, 35, 44 and 168). The genetic distance based on nucleotide COX2 calculated using Kimura 2-parameter model indicated that in the smallest genetic distance 0%, biggest 6.8% and average 2.3%. The phylogenetic tree using neighbor joining method based on the sequence of nucleotide and amino acid COX2 reveded differentiation among Tarsius from Lampung and Tarsius from Sulawesi, but could not be used to differentiate among T. dianae (from Central Sulawesi) and T. spectrum (from North Sulawesi).
Kajian Penanda Genetik Tarsius bancanus dan Tarsius spectrum dengan Sekuen D-Loop Parsial DNA Mitokondria Rini Widayanti; Dedi Duryadi Solihin
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 3 (2007): October 2007
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v12i3.2804

Abstract

The objective of this research was to study the specific genetic marker on D-loop region of Tarsius bancanus and Tarsius spectrum. The sequencing of PCR product using primer DLTARPROF on D-loop resulted in base sequence of 270 nts. Result of D-loop fragments sequencing was put on multiple alignment with other primates from Genbank with the aid of software Genetyc-Win Version 3.0 and Clustal W, and was analyzed using MEGA program version 3.1. The genetic distance was based on nucleotide D-loop, the smallest genetic distance was 0% and the biggest was 11.8% and the average was 2.3%. The phylogenetic tree using neighbor Joining Method based on some nucleotide sequence on D-loop region could not be used to differentiate between Tarsius bancanus and Tarsius spectrum.
Co-Authors Agus Priyono Amalia Rezeki Ana Sahara Aris Haryanto Aris Purwantoro Ayuda Dyah Nurekawati Charles Rangga Tabbu Dedi Duryadi Solihin Dondin Sajuthi DYAH PERWITASARI -FARAJALLAH Heri Budi Santoso Irma Padeta Joko Prastowo Kurniasih . Laksono Trisnantoro Niken Satuti Handayani Niken Satuti Nur Handayani TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Teguh Budipitojo Wayan Tunas Artama Widya Asmara Wisnu Nurcahyo Yuriadi - Yuriadi .