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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Mikologi Indonesia Jurnal Galam Bioscientiae, Jurnal ilmu ilmu Biologi
Witiyasti Imaningsih
PS Biologi ULM
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Environmental Science Immunology & microbiology Veterinary

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Lichen as bioindicator of air quality at buffer zone of Banjarbaru town Rida Yuliani; Witiyasti Imaningsih; Tri Wira Yuwati
Jurnal GALAM Vol 2, No 1 (2021): Jurnal Galam Vol. 2 No.1 2021
Publisher : Jurnal GALAM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/glm.2021.2.1.54-65

Abstract

ABSTRACTAir pollution is one of the main issues faced by urban areas. Therefore, morphological characteristic and colony coverage of lichen growing at different scale air-polluted area could become as a bioindicator of their air quality. This research aims to determine the condition of lichen in spots located at near and far from the main road (width ± 29 meter) in urban area at Banjarbaru town. This research used descriptive method with purposive sampling technique. Sample were obtained from 2 plots those were far and near the main road, each plot represented by samples from 5 different trees (densed canopy with minimum DBH at 25 cm). Lichens were taken at 50-150 cm above ground level using 20x20 cm quadrant plastic frame. Observation variables include the number and shape of colonies, color and type of thallus, and percentage of thallus cover. We determined 13 colonies from spots far from the main road (500-600 meter from main road). Lichen dominated by green to bluish color, thallus consist of crustose and foliose, and the average percentage of thallus cover was 28.01%. While from that near the main road (14-250 meter from main road), we determined 14 colonies predominantly consisted of white crustose lichens, and the average percentage of thallus cover was 10.01%. Traffic intensity showed to have significant effect on lichens community. The main difference can be seen from morphology and colony coverage. Based on this result, lichen can be used as bioindicator of air quality, especially air pollution caused by motorized vehicles.
KARAKTERISASI MORFOLOGI JAMUR ENTOMOPATOGEN DI HUTAN MANDIANGIN BANJARBARU, KALIMANTAN SELATAN N Halimah; Witiyasti Imaningsih; Mariana Mariana
Jurnal Mikologi Indonesia Vol 2, No 1 (2018): Juni 2018
Publisher : Perhimpunan Mikologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46638/jmi.v2i1.39

Abstract

Jamur entomopatogen merupakan jamur yang hidup sebagai patogen pada serangga. Jamur ini menginfeksi serangga dengan cara masuk kedalam tubuh serangga inang melalui kutikula, saluran pencernaan dan spirakel. Selain itu mampu menginfeksi hampir semua ordo serangga. Keragaman jamur ini di Indonesia belum banyak diketahui, khususnya di kawasan hutan Mandiangin, Banjarbaru, Kalimantan Selatan. Penelitian ini bertujuan mengeksplorasi dan mengkarakterisasi genus jamur entomopaten di hutan Mandiangin. Sampel diambil sebanyak enam kali pada bulan Mei-Juli 2017. Sampel yang diambil berupa jamur yang berasosiasi dengan serangga serta menunjukkan struktur reproduksi jamur dengan ciri morfologi makroskopis memiliki bentuk stroma maupun sinema. Sampel yang berhasil diperoleh kemudian diidentifikasi secara morfologi. Sebanyak empat genera jamur entomopatogen diperoleh dari hutan Mandiangin Banjarbaru,yaitu Ophiocordyceps, Hypocrella dan Cordyceps yang ditemukan pada fase seksual serta Aschersonia ditemukan pada fase aseksual.
Uji Antagonisme Kapang Endofit Tanaman Galam (Melaleuca cajuputi) terhadap Colletotrichum truncatum Nurul Huda; Witiyasti Imaningsih; Safinah Surya Hakim
Jurnal Mikologi Indonesia Vol 3, No 2 (2019): Desember 2019
Publisher : Perhimpunan Mikologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2001.42 KB) | DOI: 10.46638/jmi.v3i2.62

Abstract

Galam (Melaleuca cajuputi) adalah spesies asli dari lahan gambut yang secara alami hidup berasosiasi dengan kapang endofit. Kapang endofit dikenal karena kemampuannya sebagai biofungisida. Penelitian ini bertujuan untuk mengetahui kemampuan kapang endofit yang diisolasi dari pohon Galam untuk menghambat pertumbuhan kapang pathogen Colletotrichum truncatum (Schwein.) Andrus & W.D. Moore dan menganalisis mekanisme penghambatannya. Tahapan penelitian ini meliputi (a) isolasi kapang endofit; (b) seleksi kapang endofit; (c) uji patogenisitas dan antagonis kapang endofit; (d) uji kemampuan metabolit volatil dan non-volatil dan (e) uji mikoparasitisme. Hasil penelitian menunjukkan persentase penghambatan tertinggi pada uji antagonisme secara in-vitro dalam menghambat C. truncatum ditunjukkan oleh isolat Neurospora sp. DG 27 dengan rata-rata persentase hambatan sebesar 71,98%, diikuti oleh isolat Neurospora sp. DG 17, 53,74% dan isolat Syncephalastrum sp. AG 15, 48,28%. Uji kemampuan metabolit volatil dan non-volatil isolat Neurospora sp. DG 27 menunjukkan persentase penghambatan tertinggi terhadap kapang C. truncatum. Mekanisme penghambatan isolat Neurospora sp. DG 27 terjadi secara antibiosis sehingga terbentuk zona bening, sedangkan isolat DG 17 terjadi secara kompetisi dan isolat Syncephalastrum sp. AG 15 secara mikoparasit.
Uji Potensi Antifungi Koloid Perak Nanopartikel (AgNPs) terhadap Aspergillus niger Witiyasti Imaningsih; Ahmad Budi Junaidi; Imam Kurniawan
Bioscientiae Vol 17, No 1 (2020): BIOSCIENTIAE Volume 17 No. 1
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (394.967 KB) | DOI: 10.20527/b.v17i1.3131

Abstract

Silver nanoparticles (AgNPs) antimicrobial activity has known to be more effective than any antibiotics. Diseases which caused by pathogenic fungi has increased because of the ability of pathogenic fungi to survive against antibiotic. Silver nanoparticles have the ability to inhibit the transport material from the environment around microorganisms. The aims of this study are to measure the value of Minimal Inhibitory Concentrations (MIC) and Minimal Fungicidal Concentrations (MFC) of colloidal AgNPs to Aspergillus niger. MIC and MBC are determined based on the correlation between concentration of colloidal AgNPs and biomass of Aspergillus niger. Measurement of Aspergillus niger biomass based on treatments of Aspergillus niger spore which was applied by various concentrations of the colloidal AgNPs. Aspergillus niger spores were incubated for 5 days at room temperature above orbital shacker. MIC of colloidal AgNPs was Aspergillus niger was 1,18 µg·L-1 . MFC of colloidal AgNPs to Aspergillus niger is 60,57 µg·L-1.
Studi Banding Sifat Ketahanan Struktural Terhadap Kekeringan Antara Varietas Padi Sawah Dan Padi Gogo Berdasarkan Struktur Anatomi Daun Witiyasti Imaningsih
Bioscientiae Vol 3, No 1 (2006): Bioscientiae Volume 3 No 1
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (97.311 KB) | DOI: 10.20527/b.v3i1.151

Abstract

Experimental study has been conducted (1) to find out the structural droughtresistance traits of “Sawah” rice and “Gogo” rice , and (2) to determine the difference in leaf structure among  some Sawah rice and Gogo rice varieties. Randomly chosen samples were taken, and slides were prepared using paraffin method. Structural traits of the roots and leaves were observed: anatomical structure leaves, leaf thickness, leaf cuticle thickness, number of stomata per leaf square unit, number of trichome per leaf square unit, and number of motor cell per row.The results showed that Sawah rice and Gogo rice differed in their structural droughtresistance.
POTENSI LIMBAH KULIT UDANG SEBAGAI SUMBER NUTRISI BAGI PERTUMBUHAN Metarhizium anisopliae YANG DIUJIKAN PADA NYAMUK Aedes aegypti Nova Anisa’a; Witiyasti Imaningsih; Muhamat Muhamat
Bioscientiae Vol 17, No 1 (2020): BIOSCIENTIAE Volume 17 No. 1
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (444.155 KB) | DOI: 10.20527/b.v17i1.3920

Abstract

Shrimp shell waste is a waste from the shrimp freezing production process. Shrimp shell waste has the potential to be a source of nutrients for the growth and virulence of Metarhizium anisopliae. This study aims to calculate the amount of conidiospores and find out the virulence levels of M. anisopliae grown in PDA media with the addition of nutrient sources (yeast extract, KNO3, and media without additional nutrients). In this study the test insect used was the Aedes aegypti mosquito, which was observed every 24 hours for 96 hours. The results showed that the highest amount of conidiospores and virulences were produced by PDA media with the addition of a 0.25% source of nutrients derived from shrimp shell waste.
Isolasi dan Karakterisasi Fungi Endofit Umbi Porang (Amorphophallus muelleri Blume) sebagai Antibakteri Muhammad Fazrul Rahman; Witiyasti Imaningsih; Sasi Gendro Sari
Bioscientiae Vol 14, No 1 (2017): Bioscientiae Volume 14 No 1
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (309.323 KB) | DOI: 10.20527/b.v14i1.4016

Abstract

Medicinal plants such as porang (Amorphophallus muelleri Blume) can produce bioactive compounds from plants-associated endophytes. Therefore, medicinal plants were a sources of isolation of endophytic fungi and endophytic fungi were a sources of secondary metabolites that have anticancer, antimalarial, antimicrobial, and so on. A Porang tuber has been used as a medicine for boils, medicine for sliced wounds and medicine for wounds due to venomous animal bites. The research was aimed to carry out isolation and characterization of endophytic fungi from porang tuber, and to test the ability of endophytic fungi from porang tuber as antibacterial against gram positive (Staphylococcus aureus) and gram negative (Escherichia coli) bacteria. Isolation of endophytic fungi from porang tubers succeeded in getting five different fungi isolates. Based on macroscopic and microscopic characteristics, endophytic fungi isolated from porang tuber were members of the genus Curvularia, Penicillium, 2 isolates of Aspergillus, and 1 isolate that had not been identified. Porang endophytic fungi had antibacterial activity against S. aureus, but did not have antibacterial activity against E. coli bacteria. Endophytic fungi that had antibacterial activity were Curvularia sp., Penicillium sp., Aspergillus sp.
Karakterisasi Cendawan pada Tanah Serpentin Daerah Mandiangin Kabupaten Banjar Witiyasti Imaningsih
Bioscientiae Vol 8, No 1 (2011): Bioscientiae Volume 8 No 1
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (254.583 KB) | DOI: 10.20527/b.v8i1.185

Abstract

Serpentine soil is the land that is characterized by many heavy metal content. Three (3) genera of fungi were isolated from serpentine soil Mandiangin. Aspergillus genus with 4 species, 3 species of Penicillium and Trichoderma (2 species). Mandiangin serpentine soil areas containing nickel and chromium by an average of 3630 ug / g dry weight of soil and the average chromium content reached 828.73 ug / g dry weight soil.
PERTUMBUHAN TANAMAN JAGUNG ( ZEA MAYS ) YANG DIBERI KOMPOS TANAH GAMBUT DENGAN STIMULATOR EM4 (EFFECTIVE MICROORGANISM 4) Witiyasti Imaningsih; Hidayaturrahmah Hidayaturrahmah; Gunawan Gunawan
Bioscientiae Vol 8, No 2 (2011): Bioscientiae Volume 8 No 2
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (501.299 KB) | DOI: 10.20527/b.v8i2.192

Abstract

Pemanfaatan tanah lahan gambut sebagai kompos belum banyak dilakukan. Penelitian pendahuluan mengenai hal tersebut dilakukan dengan mengkomposkan tanah lahan gambut dengan bantuan A. niger dan stimulator EM4. Kemudian diujikan kepada tanaman jagung. Hasil penelitian menunjukkan bahwa kompos tanah gambut terbaik diperoleh dari perlakuan penambahan spora A. niger sebanyak 15 ml dengan lama pengomposan 3 minggu. Kompos tersebut memiliki kadar rasio C/N terendah dibandingkan perlakuan lain yaitu 0.46% dan kadar P total sebesar 195 ppm. Rata-rata berat kering akar, batang, daun serta pipilan jagung memiliki pola yang relatif sama, mengalami kenaikan pada pemberian kompos tanah gambut dengan penambahan spora A. niger 7.5 ml dengan lama pengomposan 1 minggu, yaitu berturut-turut 1.2gr, 0.7gr, 3.5gr dan 0.11gr. Kompos tanah gambut hanya memberikan pengaruh yang nyata pada berat kering daun. Berat kering daun tertinggi (3.5 gr) diperoleh dari pemberian kompos tanah gambut dengan penambahan spora A. niger 7.5 ml, pengomposan 1 minggu.
An Overview of Stress-Tolerant Promoting Endophytic Fungal Isolates from Hiyung Chilli Grown in South Kalimantan: Endophytic Fungi of Hiyung Chilli Peppers Witiyasti Imaningsih; Nuraeni Ekowati; Salamiah; Nuniek Ina Ratnaningtyas; Loekas Soesanto
Journal of Tropical Life Science Vol. 13 No. 1 (2023)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.13.01.18

Abstract

Endophytic fungi are a unique class of microorganisms that lives in plant tissues. These fungi could spread from roots to shoots and interact with nearly all plants in a symbiotic, mutualistic, or commensal manner. Endophytic fungi are believed to exert several roles related to plant growth, including the ability to tolerate stress, especially in Hiyung chilli plants. This plant is widely cultivated in Indonesia and is a well-known host for endophytic fungi. The Hiyung chilli is a popular variety grown in the South Kalimantan Province, which receives national recognition and is geographically certified by the Indonesian government. This wetland-cultivated chilli plant has a uniquely high capsaicin content and long fruit shelf life compared to other varieties. Although endophytic fungal isolates are prevalently found on chilli plants, their potential to influence the growth of their host remains to be proven beyond a doubt. This review emphasizes the sparse reports on the potential of these antagonistic fungi in protecting chilli plants against other pathogens by producing auxin/Indole-3-Acetic Acid (IAA) and capsaicin. The study hypothesised that the antagonistic abilities of these endophytic fungi against other fungal pathogens are related to their IAA- and capsaicin production that synergistically increase the chili plants' threshold against biotic and abiotic stress.
Co-Authors Ahmad Budi Junaidi Gunawan Gunawan Hidayaturrahmah Imam Kurniawan Loekas Soesanto Muhamat Muhamat Muhammad Fazrul Rahman N Halimah Nova Anisa’a Nuniek Ina Ratnaningtyas Nuraeni Ekowati Nurul Huda Rida Yuliani Safinah Surya Hakim Salamiah Salamiah Sasi Gendro Sari Tri Wira Yuwati