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All Journal Farmasains : Jurnal Farmasi dan Ilmu Kesehatan Bioma : Berkala Ilmiah Biologi Jurnal Kedokteran Brawijaya Pharmascience Jurnal Teknologi Laboratorium JRST (Jurnal Riset Sains dan Teknologi) JSFK (Jurnal Sains Farmasi & Klinis) FITOFARMAKA: Jurnal Ilmiah Farmasi Medica Hospitalia Jurnal Karinov Lumbung Farmasi : Jurnal Ilmu Kefarmasian Journal of Pharmaceutical Care Anwar Medika Jurnal Ilmiah Medicamento Prosiding Seminar Nasional Pengabdian Masyarakat KOPEMAS
Martina Kurnia Rohmah
Universitas Anwar Medika
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Economics, Econometrics & Finance Education Engineering Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Journal : Jurnal Kedokteran Brawijaya

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Nanosecond Pulsed Electric Fields (nsPEFs) Induce Socs1 and Socs3 but not Socs2 Gene Expressions in Hela S3 Cells Rohmah, Martina Kurnia; Lyrawati, Diana; Yano, Kenichi
Jurnal Kedokteran Brawijaya Vol 28, No 3 (2015)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2015.028.03.1

Abstract

Nanosecond Pulsed Electric Fields (nsPEFs) is one of bioelectric technologies applied widely in a number of sciences. nsPEFs cause some biological responses and known to play a role as a novel cancer therapy. However, the nsPEFs molecular mechanisms have not been fully elucidated. This study determines the effects of nsPEFs in socs (Suppressor of Cytokine Signaling) genes which are target genes of JAK/STAT signaling pathway. Through a negative feedback mechanism, SOCS proteins can suppress both cytokine signal transduction and overgrowth factor, so the cell growth is controlled. In cervix cancer, the presence of E6 and E7 HPV's oncoprotein is associated with methylation and inactivation of socs1 and socs3 genes. This mechanism is related to the increase of STAT expression and cancer prognostic. In this research, nsPEFs as much as 20 kV/cm for 80 ns was exposed over HeLa S3 cells in 4 mm cuvette. Socs1, socs2 and socs3 gene expressions were analyzed using real time PCR SYBR green and reverse transcription PCR (RT-PCR). This study shows that at 20 and 30 shots, nsPEFs significantly increase socs1 and socs3 but not socs2 gene expression. Effect of nsPEFs on socs1 and socs3 gene expression pattern is influenced by duration of post exposure incubation and each cell activity on internal cell condition. This research provides a new cancer therapy target for nsPEFs.Keywords: Bioelectric, gene expression, nsPEFs, shot, socs gene
Co-Authors Achmadita Prameswari Ambari, Yani Andre Giovano Antoni Setiawan Anwari, Farida Arista Wahyu Ningsih Azis, Rosidi Bagus Kurniawan Romadhon Damasari, Karina Putri Diana Lyrawati Djelang Zainuddin Fickri Djelang Zainuddin Fickri Djelang Zainuddin Fickria Etik Wijayanti Fickri, Djelang Zainuddin Fodeni Yolinda Herni Setyawati Herni Setyawatia Hisbiyah, A'yunil Kasifa, Wahyu Kenichi Yano Liya Fitroh Nurlaila Mila Ayu Puji Lestari Novita E. Winarni Nur Novia Dayanti Nurdianto, Arif Rahman Nurul Maulidatus Sholichah Octivani, Amellya Pahri Samsiyeh, Nur Arzy Siti Lutfiyah Muf’idah Rizki Valentino Krismonico Caesarius Wahyuni, Khurin In Yani Ambari Yani Ambari Yulianti, Ella Rahman