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All Journal Jurnal MIPA CHEMISTRY PROGRESS Journal of Tropical Soils AGRIVITA, Journal of Agricultural Science Animal Production : Indonesian Journal of Animal Production
Johnly Alfreds Rorong
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Environmental Science Mathematics Physics Veterinary

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Methane Gas Reduction Using Virgin Coconut Oil Supplementation in Rumen Fermentation through in Vitro Sondakh, Erwin Hubert Barton; Rorong, Johnly Alfreds; Kalele, Jerry Audy Donny
ANIMAL PRODUCTION Vol 17, No 3 (2015): September
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (635.769 KB) | DOI: 10.20884/1.anprod.2015.17.3.511

Abstract

This study aims to determine the effect of virgin coconut oil (VCO)supplementation into ruminant feed on the methane reduction in vitro The study used rumen fluid with forage substrate and concentrates in the ratio of 60:40. The experiement consists of five kinds of VCO treatments, namely: R0 – feed substrates without VCO; R1 – feed substrates with 2% VCO; R2 – feed substrates with 4% VCO; R3 – feed substrates with 6% VCO; and R4 – feed substrates with 8% VCO. Each treatment was done three times. The fermentation used Hohenheim Gas Test (HGT) at a temperature of 390 C for 48 hours. At the end of the fermentation, measurements of methane and microbial activity of the rumen fluid were implemented. Data obtained were analyzed using a completely randomized design, and continued with Duncan test for examining the mean differences. The results showed that methane production descreased by 18.39% - 29.7% when the feed was given 2% - 8% VCO. Based in the findings, it can be concluded that the supplementation of 2% - 8% VCO can be used a substrate for ruminant feed because it can reduce methane and does not interfere the microbial activity of the fermentation of rumen fluid through in vitro.
ANALISIS ASAM BENZOAT DENGAN PERBEDAAN PREPARASI PADA KULIT DAN DAUN KAYU MANIS (Cinnamomun burmanni) Rorong, Johnly Alfreds
CHEMISTRY PROGRESS Vol 6, No 2 (2013)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/cp.6.2.2013.3499

Abstract

Telah dilakukan analisis senyawa benzoat dalam tanaman kayu manis (Cinnamomun burmanni). Penelitian ini dilakukan, meliputi : persiapan sampel, ekstraksi pelarut, analisis secara kualitatif dan kuantitatif. Analisis kualitatif dengan reagen FeCl3 dan secara kuantitatif yaitu penentuan konsentrasi asam benzoat, secara spektrofotometri UV-Vis, pada panjang gelombang 272 nm dengan pelarut dietil eter (DE). Senyawa benzoat merupakan gabungan asam benzoat dan eugenol. Parameter penelitian adalah asam benzoat dalam kulit (K) dan daun (D) tanaman kayu manis, dengan metode perbedaan pengeringan sampel. Pengeringan dengan sinar matahari dan tanpa sinar matahari. Hasil analisis kualitatif dinyatakan positif dengan terbentuknya endapan cincin berwarna merah kecoklatan, hal itu menunjukkan keberadaan asam benzoat dalam sampel sedangkan analisis kuantitatif diperoleh konsentrasi asam benzoat dalam keempat sampel (K1;K2;D1dan D2), secara berturut-turut: 36.852; 32.763; 45.809 dan 44.571 mg/L.Analyzes have been carried of the benzoat compounds in the Cinnamomun burmanni plants. The step researches: sampling, solvents extraction, qualitative and quantitative analysis. Qualitative analysis with using FeCl3 reagent and quantitative was determined benzoat consentration according ultra violet spectrophotometric methods at 272 nm wavelength and diethyl eter solvent. The benzoat compounds was benzoic acid and eugenol combine. The parameter of researches was benzoic acid in the leaves and skin of the Cinnamomun burmanni plants using compare drying methods. Drying methods with sunray and without sunray. The research indicated that: qualitative analysis of positive results in the samples with to form solid of rings red browning color and quantitative analysis of benzoic acid consentration in the leaves and skin of the Cinnamomun burmanni plants (S1;S2;L1 and L2) : 36.852; 32.763; 45.809 dan 44.571 mg/L.
Analisis Fenolik Jerami Padi (Oryza Sativa) pada Berbagai Pelarut Sebagai Biosensitizer untuk Fotoreduksi Besi Rorong, Johnly Alfreds
Jurnal MIPA Vol 4, No 2 (2015)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jm.4.2.2015.10430

Abstract

Telah dilakukan analisis fenolik dalam Jerami Padi (Oryza sativa). Penelitian ini dilakukan beberapa tahap, meliputi : persiapan sampel; ekstraksi maserasi selama 1 x 24 jam dengan berbagai pelarut: akuades,  metanol,  etanol dan petroleum eter;  dievaporasi selama 1 jam dan dianalisis senyawa fenolik secara kualitatif menggunakan reagen Folin Ciocalteu 50% menghasilkan  ekstrak akuades (EA); ekstrak metanol (EM); ekstrak etanol (EE) dan ekstrak petroleum eter (EPE), selanjutnya dianalisis secara kuantitatif menggunakan metode spektrofotometri ultra violet visible (UV-Vis) pada panjang gelombang 750 nm. Hasil analisis menunjukkan bahwa kandungan senyawa fenolik pada ekstrak jerami padi (JP), dengan berbagai pelarut:  akuades;  metanol 40; 60 dan 80%;  etanol 25; 50; 75 dan 100%; petroleum eter,  secara berturut-turut,  sebesar : 1,285; 2,39; 2,72; 2,59; 1,406; 1,470; 1,501 2,996 dan 1,177 mg as. galat/L ekstrak sampel. Konsentrasi fenolik pada reaksi fotoreduksi besi dengan sinar UV dalam kotak cahaya menghasilkan ekstrak JP tertinggi terdapat pada ekstrak EM-60%, sebesar 2,725  mg as. galat/L ekstrak sampel, sedangkan konsentrasi Fe+2 tertinggi yang terbentuk pada reaksi fotoreduksi besi ekstrak JP dengan pelarut metanol 60% sebesar 34,50 mg/L ekstrak sampel. Kandungan fenolik tertinggi terdapat pada ekstrak  EE-100%, diikuti EM-60% dan terendah terdapat pada ekstrak  EPE. Hal  ini disebabkan senyawa fenolik bersifat semi polar dapat larut sempurna pada pelarut etanol bersifat polar sedangkan pelarut petroleum eter bersifat tidak polar. Reaksi fotoreduksi besi dalam kotak cahaya UV diperoleh ekstrak JP tertinggi pada EM-60% dan konsentrasi Fe+2 tertinggi terdapat pada EM-60%. Hal ini disebabkan ekstrak EM-60% berpotensi sebagai biosensitizer terbaik bila dibandingkan dengan pelarut yang lain.An analysis of fenolics contained in straw (Oryza sativa) has been done. The research was performed in several steps, those were sample preparation employing aquadest, methanol, ethanol, and petroleum ether, evaporation of the extracts, qualitative analysis using Folin Ciocalteu 50%, and quantitative analysis using UV-vis spectrophotometry method at 750 nm. The results showed that aquadest, 40, 60, and 80% methanol, 25, 50, 75, and 100% ethanol, and petroleum ether generated fenolics concentrations of 1.285, 2.39, 2.72, 2.59, 1.406, 1.470, 1.501, 2.996, and 1.177 mg gallic acid/L, recpectively. Extract of 60% methanol gave the highest value of photoreduction and subsequently produced highest concentration of Fe2+ which was 34.50 mg/L.
Efek Ekstrak Limbah Cair Empulur Batang Sagu Baruk (Arenga microcarpha) Terhadap Fotoreduksi Besi(III) Ginting, Haider Ali; Rorong, Johnly Alfreds; Wuntu, Audy D.
Jurnal MIPA Vol 5, No 1 (2016)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jm.5.1.2016.12285

Abstract

Telah dilakukan penelitian untuk menganalisis kandungan fenolik, flavonoid, dan tanin dari limbah cair empulur batang sagu baruk dan menguji kemampuanya dalam fotoreduksi Fe3+. Empulur batang sagu baruk diekstrak dengan pelarut akuades secara maserasi selama 2 jam. Selanjutnya ekstrak hasil maserasi dianalisis kandungan fitokimia fenolik, flavonoid dan tanin dengan menggunakan spektrofotometer UV-Vis. Pengujian fotoreduksi dilakukan pada cahaya fluorescent dengan konsentrasi 1000 mg/L selama 5 jam dengan variasi pH 4, 5, 6, 7 dan 8 serta kapasitas daya 42, 62 dan 104 Watt. Analisis ekstrak limbah cair empulur batang sagu baruk menunjukkan konsentrasi fenolik 112,04 mg asam galat/L konsentrasi flavonoid 30,10 mg kuersetin/L dan konsentrasi tanin 22,02 mg katekin/L.Hasil penelitian menunjukkan bahwa ekstrak limbah cair empulur batang sagu baruk pada pH 6 dengan kapasitas daya 104 Watt mempunyai kemampuan yang paling baik untuk mereduksi Fe3+ dibandingkan dengan ekstrak lainya.Research had been carried out to analyze the photochemicals phenolic, flavonoids, and tanins in liquid waste of pith trunk sago baruk and to test its ability in photoreduction. Pith trunk extracted with aquadest in maceration for two hours. The extracts resulted were then analyzed for phenolic, flavonoids and tanins phytochemicals using spectrophotometer UV-Vis. The photoreduction tests performed on fluorescent light with concentration of 1000 mg/L for five hours with variation pH 4, 5, 6, 7 and 8 and on energy capacities of 42, 62 dan 104 Watt. Analysis of liquid waste of pith trunk sago baruk extract showed that phenolic concentration was 112,04 mg gallic acid/L, flavonoid concentration was 30,10 mg quersetin/L and tannin concentration was 22,02 mg chatechin/L. The results showed that extract liquid waste pith trunk sago baruk at pH 6 with energy capacities 104 Watt had best ability in photoreduction Fe3+.
Aktivitas Ekstrak Buah Tomat (Lycopersicum esculentum Mill) Sebagai Penstabil Oksigen Singlet Dalam Reaksi Fotooksidasi Asam Linoleat Maong, Reynal; Rorong, Johnly Alfreds; Fatimah, Feti
Jurnal MIPA Vol 5, No 1 (2016)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jm.5.1.2016.12288

Abstract

Telah dilakukan penelitian untuk menentukan aktivitas ekstrak buah tomat apel dan tomat biasa sebagai penstabil oksigen singlet terhadap fotooksidasi asam linoleat dengan menggunakan cahaya dan eritrosin. Sampel yang digunakan adalah buah segar dari tomat apel dan tomat biasa. Penelitian ini dilakukan dengan cara ekstraksi menggunakan pelarut air panas dan etanol selama 2x24 jam. Ekstrak tomat apel dengan pelarut etanol (ETA) dan air (ATA) memiliki peroksida berturut-turut sebesar 5.738 dan 6.228 mek/kg, sedangkan ekstrak tomat biasa dengan pelarut etanol (ETB) dan air (ATB) memiliki nilai peroksida sebesar 8.677 dan 7.024 mek/kg. Ekstrak tomat apel dengan pelarut etanol (ETA) memiliki potensi penstabil oksigen singlet yang paling besar dan potensi yang paling kecil adalah ekstrak tomat biasa dengan pelarut etanol (ETB). Semakin kecil angka peroksida dari ekstrak tomat apel dan tomat biasa, semakin besar potensi penstabil oksigen singlet yang dimiliki.
PHYTOCHEMICAL ANALYSIS OF ECENG GONDOK (Eichhornia crassipessolms) OF AGRICULTURAL WASTE AS BIOSENSITIZER FOR FERRI PHOTOREDUCTION Johnly Alfreds Rorong; Sudiarso Sudiarso; Budi Prasetya; Jeany Polii-Mandang; Edi Suryanto
AGRIVITA, Journal of Agricultural Science Vol 34, No 2 (2012)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v34i2.106

Abstract

It has been done analysis for the total  phenolic, flavonoid  and tannin compounds at eceng gondok leafs (Eichornia crassipessolms). The research was done in several stages: sample preparation, maceration extraction, evaporation. The total phenolic content analysis by Folin Ciocalteu 50%, flavonoid by chloride aluminium 2% in ethanol, and tanin by vanillin 4% solution in methanol, continued by using ultra violet visible (UV-Vis) spectrophotometry methods. The results showed that the total phenolic content of eceng gondok with methanol 60% of 2.72mg galic acid/kg sample is the high total phenolic content and the lowest phenolic total  at aquades of 1.07mg galic acid/kg and the flavonoid with methanol 80% solution of 3.29mg kuersetin/kg is the high flavonoid content, and the lowest at the aquades of 1.65mg kuersetin/kg and the high condensed tannin content with methanol 80% of 0.7mg katekin /kg. The Fe2+ content photoreduction process, with methanol 60% solution showed the Fe2+  production increase of 17.67ppm, aquades of 2.94ppm. Extract that does not contain eritosin as sensitizer only produce Fe2+ of 5.6ppm and that is not illuminated only 0.06ppm   Keywords: phytochemical, agricultural waste, eceng gondok, biosentizer, ferric photoreduction mitratani    
Ferro Content in Soil and Mustard Leave (Brassica Junjea) Treated by Agricultural Waste on the Biosensitizer-Iron Photoreduction Rorong, Johnly Alfreds; Sudiarso, .; Prasetya, Budi; Mandang, Jeany Polii; Suryanto, Edi Suryanto
JOURNAL OF TROPICAL SOILS Vol. 17 No. 3: September 2012
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2012.v17i3.211-218

Abstract

Atom absorption spectrophotometer (AAS) had been used to analyzed Ferro in the soil and in the green mustardleaf (Brassica junjea) treated with phenolics extract from the agricultural wastes of clove leaf (Eugenia aromatica),rice straw (Oryza sativa) and water hyacinth leaf (Eichhornia crassipessolms), in which the phenolics as the electrondonor on the biosensitizer – iron photoreduction. Phenolics extract was obtained from varions of aquadest and 40;60; 80% methanol. The solution without extract was used as sensitizer, while the extract without illumination wasused as control. Green mustard was packed into medium polybag within it added by 2,000 mg kg-1. Soil type assample was volcanic soil in various categories, such as: soil-extract, soil NPK fertilizer extract, and soil control.Results of Ferro analysis in the clove leafs treated with 80% methanol indicated the highest increasing Fe2+ of 22.94mg kg-1. Rice straw treated with 60% methanol showed the highest increasing Fe2+ of 34.5 mg kg-1. The waterhyacinth leafs treated with 60% methanol obtained the highest increasing Fe2+ of 17.67 mg kg-1. Fe2+ concentrationat soil-clove leafs had the highest increasing of Fe2+ production for 5.6 mg kg-1. Its concentration at soil NPKfertilizer extract water hyacinth leafs showed the highest increasing of Fe2+ production for 13.39 mg kg-1. Highestconcentration of Fe2+ in the green mustard at soil NPK fertilizer extract clove leafs was 176.37 mg kg-1. Variousconcentrations and various soil categories resulted in the highest increasing Fe2+ concentration in each agricultural waste extract.Keywords: Agricultural waste extract; ferro analysis; iron photoreduction; soil category[How to Cite: Rorong JA, Sudiarso, B Prasetya, J Polii-Mandang and E Suryanto. 2012. Ferro Content in Soil and Mustard Leave (Brassica junjea) Treated by Agricultural Waste on the Biosensitizer-Iron Photoreduction. J Trop Soils, 17 (3): 211-218. doi: 10.5400/jts.2012.17.3.211][Permalink/DOI: www.dx.doi.org/10.5400/jts.2012.17.3.211]
Co-Authors Audy Denny Wuntu Budi Prasetya Budi Prasetya Edi Suryanto Feti Fatimah Haider Ali Ginting, Haider Ali Jeany Polii-Mandang Jerry Audy Donny Kalele, Jerry Audy Donny Mandang, Jeany Polii Reynal Maong, Reynal Sudiarso Sudiarso Sudiarso, . Suryanto, Edi Suryanto