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All Journal Scientific Dental Journal Journal of Indonesian Dental Association Cakradonya Dental Journal
Theodorea, Citra Fragrantia
Department Of Oral Microbiology, School Of Dentistry, Health Sciences University Of Hokkaido, Japan Department Of Oral Biology, Faculty Of Dentistry, University Of Indonesia, Indonesia
Biochemistry, Genetics & Molecular Biology Dentistry Education Health Professions Immunology & microbiology Public Health

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Pomegranate Juice Inhibits Periodontal Pathogens Biofilm in vitro Armelia Sari Widyarman; Olivia Puspita Suhalim; Dhara Nandary; Citra Fragrantia Theodorea
Scientific Dental Journal Vol. 2 No. 3 (2018): September
Publisher : Faculty of Dentistry, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26912/sdj.v2i3.2572

Abstract

Background: Pomegranate (Punica granatum) fruits are commonly regarded as medicinal plant in Indonesia, and the polyphenols found in pomegranate juice (punicalagin and ellagic acid) have been shown to have antibacterial properties. Objectives: Using monospecies and multispecies biofilms, we sought to examine the effects of pomegranate juice on the viability of three periodontal pathogens: Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, and Treponema denticola. Methods: Biofilm assays were performed using crystal violet. Pomegranate juice was obtained from pomegranates using a juicer, and the juice was then diluted into different concentrations with phosphate saline buffer. The three pathogens were cultured in both monospecies and multispecies plates. Pomegranate juice was then added to each biofilm well. These were then incubated for 1h, 6h, or 24h, after which the optical density (OD) of the biofilm mass was measured using a microplate-reader (490 nm). Biofilm without treatment was used as a negative control and 0.2% chlorhexidine gluconate as a positive control. Data were analyzed with a one-way ANOVA; the level of significance was set at p<0.05. Results: Compared to the negative control, biofilm mass was significantly decreased after treatment with pomegranate juice across all concentrations and incubation times, for both monospecies and multispecies biofilm (p<0.05). The best results were achieved with P. gingivalis biofilm, with 100% concentration (OD 0.34 ± 0.03); A. actinomycetemcomitans, 50% concentration (OD 0.22 ± 0.01); and T. denticola, with 25% concentration (OD 0.87 ± 0.08), as well as with a multispecies biofilm with a 50% concentration in 1h incubation time (OD 0.09 ± 0.02). Conclusion: Pomegranate juice effectively inhibited the biofilm formation of P. gingivalis, A. actinomycetemcomitans, and T. denticola. Pomegranate juice may therefore be used as an alternative therapy in preventing periodontal disease. Additional research should explore this effect in an environment that mimics oral cavities.
Isolation and Identification of Indonesian Lactobacillus reuteri strain from Saliva of Young Adults Armelia Sari Widyarman; Stella Pranoto; Citra Fragrantia Theodorea; Endang Winiati Bachtiar; Boy Muchlis Bachtiar
Scientific Dental Journal Vol. 2 No. 2 (2018): May
Publisher : Faculty of Dentistry, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26912/sdj.v2i2.2840

Abstract

Background: Biofilms are involved in a wide variety of microbial infections, including dental caries and periodontitis. The use of probiotics has been a promising prevention and treatment modality with which to combat biofilm-related diseases in the oral cavity. The probiotic Lactobacillus reuteri has been proven to reduce gingivitis and plaque index inside the oral cavity. These bacteria can be found in the digestive system and also in the human oral cavity. Objective: The aim of this study was to identify L. reuteri in the saliva of Indonesian young adults. Methods: Forty saliva samples were collected from 18–24 year-old Indonesian subjects. DNA extraction was performed, and then, the identification of L. reuteri was accomplished using PCR. Six subjects showed positive results. The positive samples were cultured in Rogosa Agar for 24 hours at 37°C anaerobically. Several single colonies were further cultured separately in broth medium before DNA extraction and PCR identification were performed. The four thickest bands were selected for DNA sequencing. Results: An analysis performed using BLAST showed that two of the L. reuteri strains obtained from the Indonesian saliva isolates had 96% (isolate 3.11) and 95% (isolate 5.14) values. This confirmed the presence of new strains based on average nucleotide identity (ANI). The isolate strains of 3.11 and 5.14 have been registered at DDBJ/EMBL/GenBank under the accession number LC382415 and LC382416, respectively. Conclusion: L. reuteri novel strain can be isolated from the saliva of Indonesian young adults.  Further studies involving biochemical tests and phenotypic analysis are needed to better understand these new L. reuteri strains.
The Effect of Presto Cooker as an Alternative Sterilizer Device for Standard Dental Equipment Ariadna Adisattya Djais; Citra Fragrantia Theodorea
Journal of Indonesian Dental Association Vol 2 No 1 (2019): April
Publisher : Indonesian Dental Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (929.063 KB) | DOI: 10.32793/jida.v2i1.356

Abstract

Introduction: In the suburb area of Indonesia, autoclaves as a sterilizer could not been used optimally due to inadequate electrical capacity. An alternative sterilizer such as a pressure cooker (presto) have been choosen because it has same principle as an autoclave, but it doesn’t required the electrical supply. Nevertheless, the procedure of presto in dentistry remain unclear. Objective: To obtain a standard procedure by using presto for dental instrument. Methods: The effect of presto was observed on aerobic (S. aureus ATCC 25923T), facultative anaerobes (S. mutans ATCC 25175T), anaerobes (P. gingivalis ATCC 33277T) and yeasts (C. albicans ATCC 10231T) which are exposed to the dental mirror. Each dental mirror (triplo) was dipped for 3 minutes on media containing bacteria (106 bacteria/ mL). Furthermore, the dental mirrors were cooked at presto (MAXIM, 7 L, Indonesia) which contained 500 mL of water, for 15, 30 and 45 minutes. Bacterial growth analysis were observed visually and microscopically after Gram staining. Results: In the S. aureus ATCC 25923T and C. albicans ATCC 10231T groups, up to 30 minutes the color of the media showed cloudy but remained clear when sterilized for 45 minutes. Likewise, these groups showed appereance of bacterial growth for 15-30 minutes but didn’t appear to grow in 45 minutes. While in the S. mutans ATCC 25175T and P. gingivalis ATCC 33277T groups, up to 15 minutes the color of the media showed cloudy but remained clear after being sterilized for 30 minutes. In addition, these groups showed appereance of bacterial growth for 15 minutes but absence in 30-45 minutes. Conclusion: Presto can be used as one of alternative equipment to sterilize dental instrument, effectively. The optimal killing time of bacteria and yeast was 45 minutes.
The Role of Veillonella in The Pathogenesis of Periodontitis: Systematic Review Rohmah, Devi Kartika; Soekanto, Sri Angky; Djais, Ariadna Adisattya; Theodorea, Citra Fragrantia; Sastradipura, Dewi Fatma Suniarti
Cakradonya Dental Journal Vol 17, No 2 (2025): Agustus 2025
Publisher : FKG Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/cdj.v17i2.43973

Abstract

Veillonella is a common species found in the human oral cavity that plays a significant role in biofilm ecology. Veillonella is known to play a supporting role in colonizing other, more pathogenic species. Its abundant presence in supra- and sub-gingival biofilms is thought to have an associated relationship with red-complex bacterial species. These bacteria are known to be the causative of periodontal disease. Their ability to establish this mutualistic relationship is an important factor that we need to know to expand our understanding of their relationship to the pathogenesis of periodontal disease and to develop more effective treatments.
Co-Authors Ariadna Adisattya Djais Boy M Bachtiar Dhara Nandary Djais, Ariadna Adisattya Endang Winiati Bachtiar Olivia Puspita Suhalim Rohmah, Devi Kartika Sastradipura, Dewi Fatma Suniarti Sri Angky Soekanto Stella Pranoto Widyarman, Armelia Sari