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All Journal Jurnal Teknologi Dan Industri Pangan Bionatura Dentika Dental Journal Journal of Dentistry Indonesia The Indonesian Journal of Dental Research ODONTO Dental Journal Dental Journal (Majalah Kedokteran Gigi) Interdental Jurnal Kedokteran Gigi (IJKG) Journal of Functional Food and Nutraceutical Narra J Odonto dental journal Narra X Malacca Pharmaceutics Jurnal of Syiah Kuala Dentistry Society SDJ

Boy Bachtiar

Department Of Oral Biology, Faculty Of Dentistry, Universitas Indonesia

Author-ID : 239486

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Nursing Physics Public Health

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NATURAL PRODUCTS PHARMACEUTICAL STANDARDIZATION TOWARDS PHYTOPHARMACA FOR INDONESIAN TRADITIONAL MEDICINE DEVELOPMENT: STANDARDISASI FARMASITIKAL BAHAN ALAM MENUJU FITOFARMAKA UNTUK PENGEMBANGAN OBAT TRADISIONAL INDONESIA Yuslianti, Euis Reni; Bachtiar, Boy M; Suniarti, Dewi Fatma; Sutjiatmo, Afifah B
Dentika: Dental Journal Vol. 19 No. 2 (2016): Dentika Dental Journal
Publisher : TALENTA

There is a tendency back to nature treatment in Indonesian community because modern/synthetic medicines is expensive and have a various alarming side effects. That is why natural products medicine becomes more popular, aside from mass media promotions. Researches on natural products such as herbal medicine increases along with the desire to get phytofarmaca that can compete with modern medicines. The aims of this literature review are to discuss and provides information on pharmaceutical standardization of natural products toward phytopharmaca for developing Indonesian traditional medicine. Pharmaceutical standardization will strengthen the basic of pharmaceutical science in producing safe, efficacy, and qualify traditional medicine products. The pharmaceutical standardization consists of standardizing simplicial quality, safety, efficacy, and stable formulation of medicines for use in health services.

QUANTIFICATION OF STREPTOCOCCUS SANGUINIS ISOLATED FROM DENTAL PLAQUE AND SALIVA OF SUBJECTS WITH AND WITHOUT CORONARY HEART DISEASE â€“ ANALYSIS USING REAL-TIME PCR: KUANTIFIKASI STREPTOCOCCUS SANGUINIS YANG DIISOLASI DARI PLAK DAN SALIVA GIGI SUBYEK DENGAN DAN TANPA PENYAKIT JANTUNG KORONER DENGAN MENGGUNAKAN PCR REAL-TIME Nasution, Aini Hariyani; Kemal, Yulianti; Lessang, Robert; Bachtiar, Boy
Dentika: Dental Journal Vol. 20 No. 1 (2017): Dentika Dental Journal
Publisher : TALENTA

Coronary Heart Disease (CHD) is the major cause of death in most countries in the world. Gram-positive and Gram-negative bacteria have been identified in bacteremia cases and known to have a role in various vascular diseases, including Streptococcus sanguinis which is most frequently isolated from endocarditis patients and often associated with CHD. The purpose of this study was to analyze the number of Streptococcus sanguinis isolated from dental plaque and saliva of subjects with and without CHD. Bacterial colonies isolated from the dental plaque and saliva of 16 subjects without CHD and 8 subjects with CHD were planted in Mitis salivarius agar, and then the DNA was extracted and quantified with a Real-Time PCR technique using 16S rRNA specific primers. The quantification of Real-Time PCR showed that there was a difference in the number of S. sanguinis between the two groups of subjects, but an unpaired T-test showed that the difference was not statistically significant. Furthermore, the number of S. sanguinis from dental plaque in CHD subjects tends to be higher than that of non-CHD subjects whereas the number of S. sanguinis from saliva in non-CHD subjects tends to be higher than that of CHD subjects.

ANALISIS MORFOLOGI KOLONI DAN KERAGAMAN GENOTIP STREPTOCOCCUS SANGUINIS YANG BERASAL DARI PLAK GIGI DAN SALIVA PENDERITA PENYAKIT JANTUNG KORONER: ANALYSIS OF COLONY MORPHOLOGY AND GENOTYPE DIVERSITY OF STREPTOCOCCUS SANGUINIS FROM THE DENTAL PLAQUE AND SALIVA OF CORONARY HEART DISEASE PATIENTS Yulianti Kemal; Robert Lesang; Natalina; Boy Muchlis Bachtiar; Lukman Hakim Makmun
Dentika: Dental Journal Vol. 17 No. 2 (2012): Dentika Dental Journal
Publisher : TALENTA

Penelitian sebelumnya menunjukkan akumulasi kalkulus supragingiva pada pasien dengan Penyakit Jantung Koroner(PJK) terbentuk lebih cepat dan lebih banyak dibandingkan pasien non PJK. Streptococcus sanguinis dikenal bukan sajadalam hubungannya dengan pembentukan karang gigi, tetapi juga dengan endokarditis bakterial yang dapat menimbulkankematian. Penelitian awal ini bertujuan untuk mengidentifikasi S.sanguinis yang berasal dari plak gigi dan saliva pasienPJK. Sampel berasal dari plak gigi dan saliva 17 pasien PJK yang disebar pada plat Agar Mitis Salivarius dan diinkubasidalam suasana mikroaerofilik. Total sampel adalah 50,35 berasal plak gigi, 15 dari saliva, yang diasumsikan sebagai S.sanguinis atas`dasar morfologi koloninya yang jelas, lekat, berbentuk bintang, diambil dan dikonfirmasi dengan caramenumbuhkan setiap koloni kedalam agar darah bernutrisi. Bacterial chromosomal DNA diekstraksi dan dikonformasisebagai S. sanguinis dengan menggunakan primer PCR amplifikasi spesifik regio intergenik (475-bp) dan strain standardS. sanguinis ATCC 10556. Diversiti genotip diobservasi menggunakan primer OPA2. Didapatkan 4 dari 50 isolat (8%), 1koloni dari saliva, dan 3 koloni dari plak gigi dikonformasi sebagai S. sanguinis. Sebagai kesimpulan, morfologi koloni S.sanguinis jelas, lekat, berbentuk bintang, didapatkan 4 keragaman genotip S. sanguinis di dalam plak gigi dan salivapasien PJK.

PENGARUH PEMBERIAN KOMBINASI SEL PUNCA PULPA GIGI DAN RECOMBINANT HUMAN BONE MORPHOGENETIC PROTEIN-2 TERHADAP KADAR FOSFATASE ALKALI PADA PULPA GIGI TIKUS TERINFLAMASI: COMBINATION OF RECOMBINANT HUMAN BONE MORPHOGENETIC PROTEIN- 2 AND DENTAL PULP STEM CELLS ENHANCED EXPESSION OF ALKALINE PHOSPHATASE ON INFLAMED RATâ€™S PULP Endang W Bachtiar; Mindya Yuniastuty; Aimee Monica; Boy M Bachtiar
Dentika: Dental Journal Vol. 16 No. 1 (2011): Dentika Dental Journal
Publisher : TALENTA

Pulp irritation will form reparative dentin as a defense mechanism. Currently, the materials used to help the defense pulp still have many shortcomings. Therefore, the effect of dental pulp stem cells (DPSC) and rhBMP- 2 combination on alkaline phosphatase (ALP) expresion in rat â€™s dental pulp was analyzed in this research. Lipopolysaccharide was used to irritate the pulp of 12 Sprague-Dawley rats. Materials were then applied to each group and ALP expression was performed on the first and second week. The result showed that in the first week, giving a combination of pulp stem cells with recombinant BMP-2 had not been visible to increase expression of alkaline phosphatase (ALP), a biomarker regeneration of pulp, but the increase in ALP expression occurred in the second week. In conclusion, a combination of pulp stem cells and rhBMP-2 can increase the expression of ALP on inflamed ratâ€™s pulp.

THE POTENCY OF CAJUPUTS CANDY IN MAINTAINING THE COMPETITIVE CAPACITY OF STREPTOCOCCUS SANGUINIS UPON STREPTOCOCCUS MUTANS Wijaya, Christofora Hanny; Sari, Bernadeta RE; Bachtiar, Boy M
Journal Akademik Universitas Swiss German Vol 1 No 2 (2020)
Publisher : Swiss German University & Perhimpunan Penggiat Pangan Fungsional dan Nutrasetikal Indonesia (P3FNI)

Streptococcus mutans were competing Streptococcus sanguinis in biofilm formation. As pioneer colonizer, S. sanguinis were able to control S. mutans growth. This study was aimed to explore the ability of sucrose and non-sucrose cajuputs candies (SCC and NSCC) in maintaining the antagonistic relationship between the indigenous oral flora when they grew as dual-species biofilms (S. sanguinis and S. mutans). The flavored candies (SCC and NSCC) contained cajuput and peppermint oils as the flavor which the volatile compounds had been identified. The unflavored candies were made similar to the flavored candy but excluding the flavor. The flavored candies, unflavored candies, and the control were exposed in vitro to the biofilms. The biofilms were examined for biofilm inhibition capacity, DNA amount, and the expression level of spxB mRNA. The biofilm inhibition by flavored candies were higher than the unflavored ones and were significantly different compared to the control. The flavored candies managed to decrease the total DNA amount in the biofilm, but unflavored samples did not. The qPCR assays showed that the exposure of candies did not alter the proportion of S. sanguinis DNA to S. mutans DNA in the biofilms. Meanwhile, spxB mRNA expression indicated the ability of S.sanguinis to control S. mutans growth.

Phenotype and Genotype of Enterococcus faecalis Isolated from Root Canal and Saliva of Primary Endodontic Patients Mubarak, Zaki; Asmara, Widya; Wibawa, Tri; Bachtiar, Boy
Journal of Dentistry Indonesia Vol. 23, No. 1
Publisher : UI Scholars Hub

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Objective: To investigate the phenotype and genotype of E. faecalis isolated from the root canal and saliva of primary endodontic patients with periapical lesions. Methods: Eighteen adult male and female individuals suffering from primary endodontic infection, either with or without periapical lesions, were involved in this study. Root canal scraping and saliva were collected from each subject and used for bacterial quantitation using a real-time polymerase chain reaction (RT-PCR). Enterococci were isolated using ChromAgar medium and then identified using both biochemical (Gram staining and catalase tests) and molecular biology (conventional PCR) methods. Gelatinase activity, polysaccharide capsul profile and mRNA ace expression level were determined using microbiological, biochemical and molecular biology approach, respectively. Genotype of E. faecalis was determined based on nucleotide sequence of ace and gelE genes analyzed using web-based 3730xl DNA Analyze software. Results: The results showed that except for its proportion, no significant difference was found in phenotypes (gelatinase activity and mRNA ace expression levels) and genotypes (polymorphism of Cps operon and variation of ace and gelE nucleotide sequences) of E. faecalis isolated from the root canal and saliva of primary endodontic patients had or had no periapical lesions. Conclusion: It can be concluded that E. faecalis proportion had a role in the occurrence of periapical lesions in the primary endodontic patients, but not gelatinase activity, mRNA ace expression level, Cps operon polymorphism or ace and gelE nucleotide sequence variations.

PENGHAMBATAN CAJUPUTS CANDY TERHADAP VIABILITAS KHAMIR Candida albicans SECARA IN VITRO [Inhibition of Cajuputs Candy Toward the Viability of Candida albicans by using In Vitro Assay] C. Hanny Wijaya; A. Fieki Rachmatillah; Boy M. Bachtiar
Jurnal Teknologi dan Industri Pangan Vol. 25 No. 2 (2014): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

The utilization of cajuput essential oil as a flavor in candy might give other physiological active value added. Some compounds of cajuput plant (Melaleuca cajuputi L) have been reported for their anti-microbe activities. Candida albicans is a normal commensal organism in human mouth. However, it might become virulent and responsible for oral diseases known as oral candidiasis. This study aimed to determine the effect of cajuput and peppermint oil in cajuputs candy in inhibiting the C. albicans biofilms formation by using biofilm assay and viability assay, in vitro test. Furthermore, the influence of concentration cajuput oil on its activities has also been analyzed. The result showed that the concentration of cajuput oil in cajuputs candy has been effective to inhibit the viability of C. albicans. The provision of flavor components of cajuput and peppermint oil could produce synergistic effects compared to single flavor component. The addition of cajuput oil at 0.6% has been able to inhibit the viability of C. albicans. The activities of the cajuput oil showed positive correlation with its concentration. The variable plus and minus 0.1% addition, however, did not give any significant different in its inhibition. Sensory test (hedonic test) to evaluate the flavor, aroma, and overall attributes showed there was no significant difference between 0.6 to 0.8% addition of cajuput oil upon the sensory acceptance.

POTENSI HAMBAT PERMEN LUNAK SIRIH DAN PINANG TERHADAP PEMBENTUKAN BIOFILM Streptococcus mutans Maryati Maryati; C. Hanny Wijaya; Dede R. Adawiyah; Boy M. Bachtiar
Jurnal Teknologi dan Industri Pangan Vol. 28 No. 2 (2017): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Betle leaf (Piper betle L.) essential oil and catechu nut (Areca catechu L.) extracts have been known to be able to inhibit biofilm formation of S. mutans. This research aimed to characterize the chemical compounds of betle leaf esssential oil, screen the phytochemicals in catechu nut ethanol extract, and assess the inhibitory potential of betle and catechu in chewy candy on biofilm formation by S. mutans. The experiment included preparation of extracts and chemical characterization of the raw materials, formulation of chewy candy, measurement of biofilm inhibition, and sensory evaluation of the candy. In vitro examination for inhibitory potency of betle and catechu chewy candy against biofilm formation S. mutans ATCC 31987 was performed in adhesion phase (4 hours) and active accumulation phase (18 hours). Antibacterial assay was performed in BHI broth media on microplate 96 wells. Crystal violet 0.5% was used to stain the biofilm and Optical Density (OD) was measured at λ 450 nm. The GC-MS analysis detected 32 compounds in the essential oil of betle leaf. The Betle leaf essential oil contained chavicol acetate, isoeugenol, chavibetol acetate, chavicol, and allylcatechol 3.4-diacetate, while catechu nut ethanol extract contained flavonoids and tannins. The components were possibly the inhibitory agents of S. mutans biofilm formation. Chewy candy containing 0.8% betle leaf essential oil and 2.3% catechu nut extract had effective inhibitory potential for S. mutans biofilm formation. Inhibition during adhesion phase was 74.5±0.7%, while that for accumulation phase was 60.8±1.8%. Sensory analysis suggests that the candy was slightly liked by the panelists (5±2).

RAMBUTAN HONEY PHARMACEUTICAL STANDARDIZATION FOR WOUND HEALING TREATMENT Euis Reni Yuslianti; Boy Muclish Bachtiar; Dewi Fatma Suniarti; Afifah Bambang Sutjiatmo
Bionatura Vol 17, No 1 (2018): Bionatura Maret 2018
Publisher : Direktorat Sumber Daya Akademik dan Perpustakaan

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Human wounds require proper management so they may heal faster and without complication. Honey is empirically effective to accelerate wound healing but scientifically has been proven yet as a pharmaceutical product is very limited. This study aimed to obtain rambutan honey pharmaceutical grade by setting specific and nonspecific parameters of simplicia. The research method was experiments laboratory by nonspecific parameters test (diastase enzyme activity, HMF, pH value, moisture content, ash content, glucose level, heavy metal contamination: Pb, Cd, Hg, and As, chloramphenicol, microbial contamination: coliform, and yeasts) as well as specific parameters (simplicia identity and organoleptic test). The results showed that rambutan honey proved had good quality of specific and nonspecific parameters. Rambutan honey water content has been pharmaceutical standardized according to drug standard that is to be 10.15%. Based on the results of this study it is necessary to test rambutan honey efficacy in vivo and in vitro and effects of rambutan honey topical products to humans towards phytopharmaca medicine.

Identification of Veillonella spp. on Tongue Plaque and Saliva Using Real-Time PCR Karina Dhaniarti; Fathia Agzarine Deandra; Ariadna A Djais; Boy M Bachtiar
The Indonesian Journal of Dental Research Vol 1, No 1 (2012)
Publisher : Fakultas Kedokteran Gigi, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/theindjdentres.65715

Veillonella spp., Gram-negative obligate anaerobic cocci bacteria, amounts to 3% in the oral cavity, relies on the fermentation of lactate as a carbon and energy source for growth. The bacteria are considered anti-cariogenic as they metabolize lactic acid into propionic acid which increases oral environment’s pH and reduces demineralization rate of tooth structure. Identification of Veillonella spp. using traditional methods is difficult due to the lack of conventional phenotypic and biochemical tests. Thus, the biomolecular methods are suitable for the specific detection and identification of Veillonella spp. One of the biomolecular methods that can be used is real-time Polymerase Chain Reaction (PCR), which the results can be qualitative and quantitative. This study aimed to identify Veillonella spp. in tongue plaque’s and saliva’s samples using Real -time PCR. The DNA of Veillonella spp. derived from 36 samples, 18 samples of tongue plaque and 18 samples of saliva, were extracted using a freeze-thaw method and then quantified by real-time PCR using forward primer 5’-CCG TGA TGG GAT GGA AAC TGC-3’ and reverse primer 5’-CCT TCG CCA CTG GTG TTC TTC-3’. Veillonella spp. in 18 samples of tongue plaque was 3,06 x 107 CFU/ml and in 18 saliva samples was 1,51 x 105 CFU/ml. It was concluded real-time PCR can detect Veillonella spp. from all tongue plaque’s and saliva’s samples.

Co-Authors A. Fieki Rachmatillah Abas, Abdul Hawil Afifah Bambang Sutjiatmo, Afifah Bambang Aimee Monica Aimee Monica Alphianti, Likky T. Anissha D Putri Annisa Qashdina Alghassani Ariadna A Djais Armelia Sari Widyarman Basri A. Gani Basri A.Gani C Hanny Wijaya Celik, Ismail Cherry Erlin Lindo Claudia Isabella Dede R. Adawiyah Dewi Fatma Suniarti Diana S. Ningsih Diana Setya Ningsih, Diana Setya Elisabeth A Putri Endang W Bachtiar Endang W. Bachtiar Erwin Gunawan Euis Reni Yuslianti Fathia Agzarine Deandra Ghazi M. Idroes Ghazi Mauer Idroes Hedijanti Joenoes Heriandi Sutadi I. Wayan T. Wibawan Idroes, Ghazi M. Ikrima, Abidah Ira Tanti Karina Dhaniarti Karina Natalie Natalie Karina Putri Juwita Kemala, Pati Khairan Khairan Khairan Khairan Lisa Prihastari, Lisa Lukman Hakim Makmun Lukman Hakim Makmun Maryati Maryati Maulydia, Nur B. Maulydia, Nur Balqis Megantoro, Aryo Mindia Yuniastuti Mindya Yuniastuty Mindya Yuniastuty Mochamad Fahlevi Rizal Nasution, Aini Hariyani Natalina Natalina , Ningsih, Diana S. Nur B. Maulydia Nurtami Soedarsono Pati Kemala Paulina N. Gunawan Ratna Ramadhani Refyan, Sarah Athiyyahmaulidya Ria Puspitawati Rinaldi Idroes Rintoko, Bimo Robert Lesang Robert Lesang Robert Lessang Rohmah, Devi Kartika Santi Chismirina Sari, Bernadeta RE Silvia Sulistiani Stella Pranoto Sulistiani, Silvia Sutjiatmo, Afifah B Theodorea, Citra Fragrantia Tri Wibawa Trina E. Tallei Trina E. Tallei, Trina E. Wahyu Sulistiadi Widya Asmara Widyarman, Armelia Sari Yulianti Kemal Yulianti Kemal Yundari, Yundari Yuslianti, Euis Reni Zaki Mubarak Zinatul Hayati Zuchra Helwani

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