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All Journal Jurnal Teknologi Dan Industri Pangan Bionatura Dentika Dental Journal The Indonesian Journal of Dental Research Dental Journal (Majalah Kedokteran Gigi) Scientific Dental Journal Journal of Functional Food and Nutraceutical Jurnal of Syiah Kuala Dentistry Society

Boy M Bachtiar

Department Of Oral Biology, Faculty Of Dentistry, Universitas Indonesia

Author-ID : 239486

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Education Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Nursing

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NATURAL PRODUCTS PHARMACEUTICAL STANDARDIZATION TOWARDS PHYTOPHARMACA FOR INDONESIAN TRADITIONAL MEDICINE DEVELOPMENT: STANDARDISASI FARMASITIKAL BAHAN ALAM MENUJU FITOFARMAKA UNTUK PENGEMBANGAN OBAT TRADISIONAL INDONESIA Yuslianti, Euis Reni; Bachtiar, Boy M; Suniarti, Dewi Fatma; Sutjiatmo, Afifah B
Dentika: Dental Journal Vol. 19 No. 2 (2016): Dentika Dental Journal
Publisher : TALENTA

There is a tendency back to nature treatment in Indonesian community because modern/synthetic medicines is expensive and have a various alarming side effects. That is why natural products medicine becomes more popular, aside from mass media promotions. Researches on natural products such as herbal medicine increases along with the desire to get phytofarmaca that can compete with modern medicines. The aims of this literature review are to discuss and provides information on pharmaceutical standardization of natural products toward phytopharmaca for developing Indonesian traditional medicine. Pharmaceutical standardization will strengthen the basic of pharmaceutical science in producing safe, efficacy, and qualify traditional medicine products. The pharmaceutical standardization consists of standardizing simplicial quality, safety, efficacy, and stable formulation of medicines for use in health services.

ANALISIS MORFOLOGI KOLONI DAN KERAGAMAN GENOTIP STREPTOCOCCUS SANGUINIS YANG BERASAL DARI PLAK GIGI DAN SALIVA PENDERITA PENYAKIT JANTUNG KORONER: ANALYSIS OF COLONY MORPHOLOGY AND GENOTYPE DIVERSITY OF STREPTOCOCCUS SANGUINIS FROM THE DENTAL PLAQUE AND SALIVA OF CORONARY HEART DISEASE PATIENTS Yulianti Kemal; Robert Lesang; Natalina; Boy Muchlis Bachtiar; Lukman Hakim Makmun
Dentika: Dental Journal Vol. 17 No. 2 (2012): Dentika Dental Journal
Publisher : TALENTA

Penelitian sebelumnya menunjukkan akumulasi kalkulus supragingiva pada pasien dengan Penyakit Jantung Koroner(PJK) terbentuk lebih cepat dan lebih banyak dibandingkan pasien non PJK. Streptococcus sanguinis dikenal bukan sajadalam hubungannya dengan pembentukan karang gigi, tetapi juga dengan endokarditis bakterial yang dapat menimbulkankematian. Penelitian awal ini bertujuan untuk mengidentifikasi S.sanguinis yang berasal dari plak gigi dan saliva pasienPJK. Sampel berasal dari plak gigi dan saliva 17 pasien PJK yang disebar pada plat Agar Mitis Salivarius dan diinkubasidalam suasana mikroaerofilik. Total sampel adalah 50,35 berasal plak gigi, 15 dari saliva, yang diasumsikan sebagai S.sanguinis atas`dasar morfologi koloninya yang jelas, lekat, berbentuk bintang, diambil dan dikonfirmasi dengan caramenumbuhkan setiap koloni kedalam agar darah bernutrisi. Bacterial chromosomal DNA diekstraksi dan dikonformasisebagai S. sanguinis dengan menggunakan primer PCR amplifikasi spesifik regio intergenik (475-bp) dan strain standardS. sanguinis ATCC 10556. Diversiti genotip diobservasi menggunakan primer OPA2. Didapatkan 4 dari 50 isolat (8%), 1koloni dari saliva, dan 3 koloni dari plak gigi dikonformasi sebagai S. sanguinis. Sebagai kesimpulan, morfologi koloni S.sanguinis jelas, lekat, berbentuk bintang, didapatkan 4 keragaman genotip S. sanguinis di dalam plak gigi dan salivapasien PJK.

PENGARUH PEMBERIAN KOMBINASI SEL PUNCA PULPA GIGI DAN RECOMBINANT HUMAN BONE MORPHOGENETIC PROTEIN-2 TERHADAP KADAR FOSFATASE ALKALI PADA PULPA GIGI TIKUS TERINFLAMASI: COMBINATION OF RECOMBINANT HUMAN BONE MORPHOGENETIC PROTEIN- 2 AND DENTAL PULP STEM CELLS ENHANCED EXPESSION OF ALKALINE PHOSPHATASE ON INFLAMED RATâ€™S PULP Endang W Bachtiar; Mindya Yuniastuty; Aimee Monica; Boy M Bachtiar
Dentika: Dental Journal Vol. 16 No. 1 (2011): Dentika Dental Journal
Publisher : TALENTA

Pulp irritation will form reparative dentin as a defense mechanism. Currently, the materials used to help the defense pulp still have many shortcomings. Therefore, the effect of dental pulp stem cells (DPSC) and rhBMP- 2 combination on alkaline phosphatase (ALP) expresion in rat â€™s dental pulp was analyzed in this research. Lipopolysaccharide was used to irritate the pulp of 12 Sprague-Dawley rats. Materials were then applied to each group and ALP expression was performed on the first and second week. The result showed that in the first week, giving a combination of pulp stem cells with recombinant BMP-2 had not been visible to increase expression of alkaline phosphatase (ALP), a biomarker regeneration of pulp, but the increase in ALP expression occurred in the second week. In conclusion, a combination of pulp stem cells and rhBMP-2 can increase the expression of ALP on inflamed ratâ€™s pulp.

THE POTENCY OF CAJUPUTS CANDY IN MAINTAINING THE COMPETITIVE CAPACITY OF STREPTOCOCCUS SANGUINIS UPON STREPTOCOCCUS MUTANS Wijaya, Christofora Hanny; Sari, Bernadeta RE; Bachtiar, Boy M
Journal Akademik Universitas Swiss German Vol 1 No 2 (2020)
Publisher : Swiss German University & Perhimpunan Penggiat Pangan Fungsional dan Nutrasetikal Indonesia (P3FNI)

Streptococcus mutans were competing Streptococcus sanguinis in biofilm formation. As pioneer colonizer, S. sanguinis were able to control S. mutans growth. This study was aimed to explore the ability of sucrose and non-sucrose cajuputs candies (SCC and NSCC) in maintaining the antagonistic relationship between the indigenous oral flora when they grew as dual-species biofilms (S. sanguinis and S. mutans). The flavored candies (SCC and NSCC) contained cajuput and peppermint oils as the flavor which the volatile compounds had been identified. The unflavored candies were made similar to the flavored candy but excluding the flavor. The flavored candies, unflavored candies, and the control were exposed in vitro to the biofilms. The biofilms were examined for biofilm inhibition capacity, DNA amount, and the expression level of spxB mRNA. The biofilm inhibition by flavored candies were higher than the unflavored ones and were significantly different compared to the control. The flavored candies managed to decrease the total DNA amount in the biofilm, but unflavored samples did not. The qPCR assays showed that the exposure of candies did not alter the proportion of S. sanguinis DNA to S. mutans DNA in the biofilms. Meanwhile, spxB mRNA expression indicated the ability of S.sanguinis to control S. mutans growth.

PENGHAMBATAN CAJUPUTS CANDY TERHADAP VIABILITAS KHAMIR Candida albicans SECARA IN VITRO [Inhibition of Cajuputs Candy Toward the Viability of Candida albicans by using In Vitro Assay] C. Hanny Wijaya; A. Fieki Rachmatillah; Boy M. Bachtiar
Jurnal Teknologi dan Industri Pangan Vol. 25 No. 2 (2014): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

The utilization of cajuput essential oil as a flavor in candy might give other physiological active value added. Some compounds of cajuput plant (Melaleuca cajuputi L) have been reported for their anti-microbe activities. Candida albicans is a normal commensal organism in human mouth. However, it might become virulent and responsible for oral diseases known as oral candidiasis. This study aimed to determine the effect of cajuput and peppermint oil in cajuputs candy in inhibiting the C. albicans biofilms formation by using biofilm assay and viability assay, in vitro test. Furthermore, the influence of concentration cajuput oil on its activities has also been analyzed. The result showed that the concentration of cajuput oil in cajuputs candy has been effective to inhibit the viability of C. albicans. The provision of flavor components of cajuput and peppermint oil could produce synergistic effects compared to single flavor component. The addition of cajuput oil at 0.6% has been able to inhibit the viability of C. albicans. The activities of the cajuput oil showed positive correlation with its concentration. The variable plus and minus 0.1% addition, however, did not give any significant different in its inhibition. Sensory test (hedonic test) to evaluate the flavor, aroma, and overall attributes showed there was no significant difference between 0.6 to 0.8% addition of cajuput oil upon the sensory acceptance.

POTENSI HAMBAT PERMEN LUNAK SIRIH DAN PINANG TERHADAP PEMBENTUKAN BIOFILM Streptococcus mutans Maryati Maryati; C. Hanny Wijaya; Dede R. Adawiyah; Boy M. Bachtiar
Jurnal Teknologi dan Industri Pangan Vol. 28 No. 2 (2017): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Betle leaf (Piper betle L.) essential oil and catechu nut (Areca catechu L.) extracts have been known to be able to inhibit biofilm formation of S. mutans. This research aimed to characterize the chemical compounds of betle leaf esssential oil, screen the phytochemicals in catechu nut ethanol extract, and assess the inhibitory potential of betle and catechu in chewy candy on biofilm formation by S. mutans. The experiment included preparation of extracts and chemical characterization of the raw materials, formulation of chewy candy, measurement of biofilm inhibition, and sensory evaluation of the candy. In vitro examination for inhibitory potency of betle and catechu chewy candy against biofilm formation S. mutans ATCC 31987 was performed in adhesion phase (4 hours) and active accumulation phase (18 hours). Antibacterial assay was performed in BHI broth media on microplate 96 wells. Crystal violet 0.5% was used to stain the biofilm and Optical Density (OD) was measured at λ 450 nm. The GC-MS analysis detected 32 compounds in the essential oil of betle leaf. The Betle leaf essential oil contained chavicol acetate, isoeugenol, chavibetol acetate, chavicol, and allylcatechol 3.4-diacetate, while catechu nut ethanol extract contained flavonoids and tannins. The components were possibly the inhibitory agents of S. mutans biofilm formation. Chewy candy containing 0.8% betle leaf essential oil and 2.3% catechu nut extract had effective inhibitory potential for S. mutans biofilm formation. Inhibition during adhesion phase was 74.5±0.7%, while that for accumulation phase was 60.8±1.8%. Sensory analysis suggests that the candy was slightly liked by the panelists (5±2).

RAMBUTAN HONEY PHARMACEUTICAL STANDARDIZATION FOR WOUND HEALING TREATMENT Euis Reni Yuslianti; Boy Muclish Bachtiar; Dewi Fatma Suniarti; Afifah Bambang Sutjiatmo
Bionatura Vol 17, No 1 (2018): Bionatura Maret 2018
Publisher : Direktorat Sumber Daya Akademik dan Perpustakaan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Human wounds require proper management so they may heal faster and without complication. Honey is empirically effective to accelerate wound healing but scientifically has been proven yet as a pharmaceutical product is very limited. This study aimed to obtain rambutan honey pharmaceutical grade by setting specific and nonspecific parameters of simplicia. The research method was experiments laboratory by nonspecific parameters test (diastase enzyme activity, HMF, pH value, moisture content, ash content, glucose level, heavy metal contamination: Pb, Cd, Hg, and As, chloramphenicol, microbial contamination: coliform, and yeasts) as well as specific parameters (simplicia identity and organoleptic test). The results showed that rambutan honey proved had good quality of specific and nonspecific parameters. Rambutan honey water content has been pharmaceutical standardized according to drug standard that is to be 10.15%. Based on the results of this study it is necessary to test rambutan honey efficacy in vivo and in vitro and effects of rambutan honey topical products to humans towards phytopharmaca medicine.

Identification of Veillonella spp. on Tongue Plaque and Saliva Using Real-Time PCR Karina Dhaniarti; Fathia Agzarine Deandra; Ariadna A Djais; Boy M Bachtiar
The Indonesian Journal of Dental Research Vol 1, No 1 (2012)
Publisher : Fakultas Kedokteran Gigi, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/theindjdentres.65715

Veillonella spp., Gram-negative obligate anaerobic cocci bacteria, amounts to 3% in the oral cavity, relies on the fermentation of lactate as a carbon and energy source for growth. The bacteria are considered anti-cariogenic as they metabolize lactic acid into propionic acid which increases oral environment’s pH and reduces demineralization rate of tooth structure. Identification of Veillonella spp. using traditional methods is difficult due to the lack of conventional phenotypic and biochemical tests. Thus, the biomolecular methods are suitable for the specific detection and identification of Veillonella spp. One of the biomolecular methods that can be used is real-time Polymerase Chain Reaction (PCR), which the results can be qualitative and quantitative. This study aimed to identify Veillonella spp. in tongue plaque’s and saliva’s samples using Real -time PCR. The DNA of Veillonella spp. derived from 36 samples, 18 samples of tongue plaque and 18 samples of saliva, were extracted using a freeze-thaw method and then quantified by real-time PCR using forward primer 5’-CCG TGA TGG GAT GGA AAC TGC-3’ and reverse primer 5’-CCT TCG CCA CTG GTG TTC TTC-3’. Veillonella spp. in 18 samples of tongue plaque was 3,06 x 107 CFU/ml and in 18 saliva samples was 1,51 x 105 CFU/ml. It was concluded real-time PCR can detect Veillonella spp. from all tongue plaque’s and saliva’s samples.

Elderly nutritional status effection salivary anticandidal capacity against Candida albicans Ria Puspitawati; Nurtami Soedarsono; Elisabeth A Putri; Anissha D Putri; Boy M Bachtiar
Dental Journal (Majalah Kedokteran Gigi) Vol. 44 No. 2 (2011): June 2011
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Elderly often suffer malnutrition and oral candidiasis. Candida albicans (C. albicans) which is the most prominent cause of oral candidiasis, is one of commensal oral micro-flora. Nutritional status affect the characteristic of saliva. Saliva is the regulator in the development of C. albicans from comensal into pathogen. Purpose: The purpose of this study was to determining the correlation between elderly nutritional status with salivary total protein and its activity in inhibiting C. albicans growth and biofilm formation. Methods: Using mini nutritional assessment 30 elderly were classified into normal and malnutrition groups. Total protein of unstimulated saliva was measured using Bradford protein assay. The colony forming unit (CFU) of C. albicans was counted on 72 hours on SDA cultures without (control) or with 2 hour saliva exposure. Biofilm formation was analyzed from the optical density of 10–5 C. albicans suspension without saliva exposure (control) or with exposure of 10.000 μg/ml saliva and incubated in 37° C for 2 days. The suspension was put into 96 well plates, stained with crystal-violet dye, and analyzed using microplate reader. Differences between groups were analyzed using independent t-test or Kruskall-Wallis. Correlation between variables was analyzed using Spearman test. Results: Salivary total protein of normal elderly (1.113.5 ± 1.1143.3) was higher than those of malnutrition (613.6 ± 253.6) but not statistically significant (p > 0.05). The CFU of C. albicans exposed to saliva of normal samples (2.060 cfu/ml) was significantly lower than control (24.100 cfu/ml) and those exposed to malnutrition saliva (5.513.3 cfu/ml). C. albicans biofilm formation is highest in controls (0.177), lower in those exposed to malnourished saliva (0.151) and lowest in those exposed to saliva of good nourished elderly (0.133). Conclusion: Although does not cause significant decrease of salivary total protein, malnutrition in elderly results in lower capacity of saliva in inhibiting the growth and declining the virulence of C. albicans.Latar belakang: Lansia sering menderita malnutrisi dan kandidiasis oral. Candida albicans yang merupakan penyebab utama terjadinya candidiasis, adalah salah satu mikroflora rongga mulut yang bersifat konvensional. Malnutrisi memengaruhi karakteristik saliva. Saliva merupakan regulator utama perkembangan Candida albicans (C. albicans) dari sifat konvensional menjadi bersifat patogen.Tujuan: Tujuan dari penelitian ini adalah untuk menentukan korelasi antara status gizi lansia dengan total protein dan aktivitas saliva dalam menghambat pertumbuhan dan pembentukan biofilm C. albicans. Metode: Menggunakan mini nutritional assessment, 30 lansia diklasifikasikan menjadi kelompok gizi baik dan gizi buruk. Total protein unstimulated saliva diukur dengan metode Bradford protein assay. Colony forming unit (CFU) dihitung pada kultur C. albicans pada saburaud dextrose agar (SDA) berusia 72 jam yang sebelumnya telah dipaparkan saliva selama 2 jam. Kontrol adalah kultur C. albicans tanpa paparan saliva. Pembentukan biofilm adalah pengukuran optical density suspensi 10–5 C. albicans tanpa paparan saliva (kontrol) atau dengan paparan saliva 10.000 μg/ml dan diinkubasi pada suhu 37° C selama 2 hari. Suspensi tersebut kemudian dimasukkan ke dalam 96 well plates, diberi pewarna crystal violet, dan diukur menggunakan microplate reader. Analisis data menggunakan uji beda t Independen atau Kruskall-Wallis, dan uji korelasi Spearman. Hasil: Total protein saliva lansia gizi baik (1.113,5 ± 1.1143,3) lebih tinggi dari lansia gizi buruk (613,6 ± 253,6) tetapi tidak bermakna secara statistik (p > 0,05). Pembentukan koloni C. albicans yang terpapar saliva lansia gizi baik (2.060 cfu/ml) secara signifikan lebih rendah dari kontrol (24.100 cfu/ml) dan daripada yang terpapar saliva lansia gizi buruk (5.513,3 cfu/ml). Pembentukan biofilm C. albicans tetinggi pada kontrol (0,177), lebih rendah pada yang terpapar saliva gizi buruk (0,151) dan terrendah pada yang terpapar saliva gizi baik (0,133). Kesimpulan: Meskipun malnutrisi tidak menyebabkan penurunan total protein saliva lansia, tetapi menurunkan kapasitasnya dalam menghambat pertumbuhan dan mengurangi virulensi C. albicans.

The frequency of bottle feeding as the main factor of baby bottle tooth decay syndrome Mochamad Fahlevi Rizal; Heriandi Sutadi; Boy M Bachtiar; Endang W Bachtiar
Dental Journal (Majalah Kedokteran Gigi) Vol. 43 No. 1 (2010): March 2010
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Dental caries remains as main problem in Indonesia and its prevalence is high (90.05%). However, there is no appropriate data that can be used to analyze dental caries in toddlers, especially baby bottle tooth decay syndrome (BBTD), though the number of BBTD cases is high in some pediatric dental clinics (90% of patients visiting the clinics). Even though some factors have already been considered to be the risk factor of BBTD, the main risk factor of BBTD is still unknown, especially BBTD in Indonesia. Purpose: This research was aimed to obtain data relating with bottle-feeding habit in 3-5 year old children in Indonesia and its caries risk. Method: The study was an observational research conducted with clinical examination through caries status (deft) of each child deserved by pediatric dentists and through questionnaire distributed to parents to examine the risk factor of BBTD. Observation was conducted on 62 children in the range of age 3 to 5 years old with bottle-feeding habit. Result: The results revealed that status of caries was various. The data showed that the frequency of bottle feeding more than twice could trigger BBTD 2.27 times higher than other factors such as the use of bottle feeding as a pacifier prior sleeping, the period of bottle-feeding, and the breast-feeding experience. Conclusion: though milk as subtract can possibly become a factor triggering caries, the frequency of bottle-feeding is highly considered as main factor. Since it could modulated the bacterial colonization on dental surface, which affects its virulence.Latar belakang: Karies masih menjadi masalah utama di Indonesia. Dalam praktek sehari-hari prevalensi karies masih sangat tinggi (90.05%). Belum ada data yang memadai dalam penelaahan karies yang spesifik pada anak balita selama ini khususnya kasus sindroma karies botol (SKB) sementara itu kasus SKB ditemukan sangat tinggi di beberapa klinik gigi anak (90% dari jumlah pasien yang datang ke klinik). Beberapa faktor menjadi resiko kejadian SKB dan belum diketahui faktor resiko utama kejadian karies khususnya di Indonesia. Tujuan: Penelitian ini dilakukan guna mendapatkan data yang berhubungan dengan kebiasaan minum susu botol pada anak usia 3-5 tahun di Indonesia serta resiko kejadian karies yang ditimbulkannya. Metode: Penelitian ini merupakan penelitian observasional yang dilakukan dengan metode pemeriksaan klinis melalui pencatatan status karies (deft) setiap anak oleh dokter gigi anak serta pengisian kuesioner yang dilakukan oleh orang tua untuk menentukan faktor resiko kejadian SKB. Pengamatan dilakukan pada 62 orang anak usia 3-5 tahun yang mempunyai kebiasaan minum susu botol sesuai dengan kriteria inklusi. Hasil: Hasil pemeriksaan klinis dan kuesioner memberikan gambaran status karies yang bervariasi. Data yang didapat dari penelitian ini menjelaskan, bahwa frekuensi minum susu botol lebih dari dua kali menyebabkan SKB 2.27 kali lebih besar dibandingkan dengan beberapa faktor lain, seperti menjadikannya pengantar tidur, lamanya mengonsumsi, dan riwayat minum ASI. Kesimpulan: Susu sebagai subtrat mungkin dapat dijadikan alasan kejadian karies akan tetapi yang menjadi resiko utama kejadian adalah frekuensi konsumsi susu botol itu sendiri. Kondisi ini dapat dihubungkan dengan modulasi substrat terhadap perkembangan kolonisasi bakteri di permukaan gigi, sehingga secara tidak langsung juga mempengaruhi virulensinya.

Co-Authors A. Fieki Rachmatillah Afifah Bambang Sutjiatmo, Afifah Bambang Aimee Monica Aimee Monica Anissha D Putri Annisa Qashdina Alghassani Ariadna A Djais Basri A. Gani Basri A.Gani C Hanny Wijaya Dede R. Adawiyah Dewi Fatma Suniarti Elisabeth A Putri Endang W Bachtiar Endang W Bachtiar Endang W Bachtiar Endang Winiati B Endang Winiati Bachtiar Euis Reni Yuslianti Fathia Agzarine Deandra Heriandi Sutadi I. Wayan T. Wibawan Karina Dhaniarti Lukman Hakim Makmun Maryati Maryati Mindya Yuniastuty Mindya Yuniastuty Mochamad Fahlevi Rizal Natalina Nurtami Soedarsono Ria Puspitawati Robert Lesang Santi Chismirina Sari, Bernadeta RE Stella Pranoto Sutjiatmo, Afifah B Theodorea, Citra Fragrantia Widyarman, Armelia Sari Yulianti Kemal Yuslianti, Euis Reni Zaki Mubarak Zinatul Hayati

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