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Isolasi Flavonoid dari Daun Durian (Durio Zibethinus Murr., Bombacaceae) Muhamad Insanu; Komar Ruslan; Irda Fidrianny; Sienny Wijaya
Acta Pharmaceutica Indonesia Vol. 36 No. 1 & 2 (2011)
Publisher : School of Pharmacy Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Daun durian (Durio zibethinus Murr., Bombacaceae) secara tradisional banyak digunakan untuk menurunkan demam. Penelitian dan publikasi mengenai kandungan kimia daun durian masih sangat terbatas. Penelitian ini dilakukan untuk menelaah kandungan kimia daun durian. Simplisia daun durian diekstraksi secara maserasi menggunakan pelarut berturut-turut n-heksana, etil asetat dan etanol. Ekstrak etanol difraksinasi menggunakan metode ekstraksi cair-cair dengan menggunakan pelarut eter, etil asetat dan butanol. Fraksi butanol dimurnikan secara kromatografi kertas preparatif. Sedangkan fraksi eter dimurnikan secara kromatografi lapis tipis preparatif. Isolat dikarakterisasi dengan penampak bercak spesifik, spektrofotometri ultraviolet - sinar tampak dan pereaksi geser. Dari ekstrak etanol diperoleh isolat S yang menunjukkan dua puncak pada 257 tim, 357 nm dan isolat W yang menunjukkan puncak pada 268 nm dan 313 nm. Isolat S merupakan golongan senyawa ilavonol 3-OH tersubstitusi dengan gugus OH pada atom C-5, C-7, C-3' dan C-4'. Isolat W merupakan senyawa golongan flavon dengan gugus OH pada atom C-5, C-77 dan C-4'.
Antioxidant Activity, Sun Protection Factor and Phytochemical Properties of Different Parts Sawo Walanda (Pouteria Campechiana (Kunth.) B.) Extract Fitriansyah, Sani Nurlaela; Fadhilah, Syifa; Ruslan, Komar; Hartati, Rika; Fidrianny, Irda
Indonesian Journal of Pharmaceutical Science and Technology Suppl. 5, No. 2 (2023) Special Issue for The 3rd Bandung International Teleconference on Pharmacy (B
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/ijpst.v0i0.50584

Abstract

AbstractUV radiation can lead production of Reactive Oxygen Species (ROS) on the skin and can cause negative effects1. Sun protector and antioxidant ingredients are needed to protect the skin2. Phenolic compounds and flavonoids have been used as sun protectors and as antioxidants3. Pouteria campechiana is known for its high abundance of phenolic compounds4. This study reported the phytochemical group, total phenol and total flavonoid content, antioxidant activity, and sun protector factor of ethanol extracts of pulp, seed, leaves, and twigs of Pouteria campechiana. Determination of antioxidant activity, sun protection factor, and total phenol and total flavonoid content were carried out using UV-visible spectrophotometry. Crude drugs and extract of pulp, seed, twig, and leaves P.campechiana containing phenolic group, tannin, and flavonoid. The leaf extract had the highest total phenolic content, while the highest total flavonoid content was in the seed extract. All of the extracts tested had very strong antioxidant activity indicated by the Antioxidant Activity Index (AAI) to DPPH value, >2. At a concentration of 1000 µg/mL, the leaf extract showed the highest SPF value, 16.01 ± 0.38.The conclusion, the leaf extract had the potential to further as a natural antioxidant, and sun protector. AbstrakPaparan radiasi UV dapat meningkatkan produksi Reactive Oxygen Species (ROS) sebagai radikal bebas dan dapat menimbulkan efek negatif pada kulit. Dibutuhkan senayawa aktif sebagai fotoprotektor dan senyawa penangkal radikal bebas yang disebut antioksidan untuk melindungi kulit. Golongan senyawa fenol dan flavonoid dapat menyerap radiasi UV dan menangkal radikal bebas. Pouteria campechiana dilaporkan mengandung senyawa fenol. Penelitian ini melaporkan golongan senyawa kimia, kadar fenol dan flavonoid total, aktivitas antioksidan terhadap DPPH dan aktivitas fotoprotektor yang menggunakan parameter nilai Sun Protector Factor (SPF) dari ekstrak etanol daging buah, biji, ranting dan daun Poteria campechiana. Ativitas antioksidan, nilai SPF, kadar fenol total, dan flavonoid total diukur secara spektrofotometri UV-Sinar tampak. Hasil penelitin menunjukkan simplisia dan ekstrak etanol daging buah, biji, ranting dan daun P.campechiana mengandung golongan senyawa fenoli, tanin, dan flavonoid. Ekstrak etanol daun memiliki kadar fenol total tertinggi, sedangkan kadar flavonoid total tertinggi ada pada ekstrak etanol biji. Keempat ekstrak tergolong pada antioksidan sangat kuat, dengan parameter nilai AAI >2. Pada konsentrasi 1000 µg/mL, ekstrak daun menunjukkan nilai SPF tertinggi yaitu sebesar 16,01 ± 0,38. Kesimpulan, ekstrak etanol daun P.campechiana lebih berpotensi untuk dikembangkan sebagai bahan baku aktif antioksidan alami dan fotoprotektor.
PHYTOCHEMICAL STUDY OF CELL CULTURE JATROPHA CURCAS ELFAHMI, ELFAHMI; ARTRI; RUSLAN, KOMAR
BIOTROPIA Vol. 18 No. 1 (2011): BIOTROPIA Vol. 18 No. 1 June 2011
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2011.18.1.137

Abstract

Jatropha curcas belongs to the Euphorbiaceae family which has potential economically. This plant has been reported to contain toxic compounds such as curcin and phorbol ester and its derivatives. These compounds may become a problem if J. curcas will be explored as a source of biofuel. In order to provide safety plants, the research on the study of phytochemical and initiation of cell and organ culture have been carried out. J curcas which has been collected from different regions in Indonesia showed to contain relatively the same profile of chemical contents. Dominant compounds that were detected by GCMS are hidrocarbon such as  2-heptenal, decadienal, hexsadecane, pentadecane, cyclooctane etc, fatty acid such as oktadecanoate acid, etthyl linoleate, ethyl stearate, heksadecanoate acid and steroid such as stigmasterol, fucosterol, sitosterol. No phorbol ester and its derivatives have been detected yet by the GCMS method. Callus and suspension cultures of J. curcas have been established to be used for further investigation.   Keywords: Jatropha curcas, phytochemical, biofuel, cell culture, steroid, hydrocarbon, fatty acid