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All Journal Jurnal Pengabdian Magister Pendidikan IPA Jurnal Ilmiah Inovasi Abdimas Universal Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Parawita Dewanti
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Environmental Science Health Professions Law, Crime, Criminology & Criminal Justice Social Sciences Other

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Journal : Jurnal Bioteknologi

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ANALYSIS OF BBM, LEC, AND SERK EXPRESSIONS IN CALLUS OF SUGARCANE (Saccharum officinarum L.) AT SOMATIC EMBRYOGENESIS DEVELOPMENT STAGES Parawita Dewanti
Jurnal Bioteknologi dan Biosains Indonesia Vol. 9 No. 1 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

Callus formed in somatic embryogenesis is divided into two types, embryogenic and non-embryogenic callus. Embryogenic callus can be distinguished by the expression of genes as markers related to somatic embryogenesis which are expected to be used as markers to detect callus that has embryogenic capabilities in sugarcane. The aim of this study was to determine the expressions of somatic embryogenesis-related genes in sugarcane. The genes analysis was carried out using somatic embryogenesis callus and using a kit for RNA analysis. Results showed that there were expressions of Baby Boom (BBM), Leafy Cotyledon (LEC), dan Somatic Embryogenesis Receptor Like-Kinase (SERK) gene with specific primer of those three genes, which were collected from embryogenic callus in mass, globular, scutelar, and coleoptilar pre-embryo stages, meanwhile there were no expressions of those genes collected from non-embryogenic callus. Amplification result from PCR product of cDNA using three gene primers detected were in 500 bp for BBM, 400 bp for LEC, and 700 bp for SERK gene.
Co-Authors Dwi Merry Christmarini Robin Fitri Lailatul Qomariyah Luh Putu Ratna Sundari Ratnasari, Tri Restanto, Didik Pudji Siti Komariyah Tri Handoyo Yani Corvianindya Rahayu