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All Journal HAYATI Journal of Biosciences Agrikultura Jurnal Ilmu Dasar Planta Tropika Jurnal Ilmiah Rekayasa Pertanian dan Biosistem Biosaintifika: Journal of Biology & Biology Education Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Jurnal Pengabdian Magister Pendidikan IPA Reswara: Jurnal Pengabdian Kepada Masyarakat Jurnal Pengabdian Kepada Masyarakat (JPKM) Tabikpun Golden Ratio of Mapping Idea and Literature Format Journal of Applied Agricultural Science and Technology Karimah Tauhid Jurnal Cakrawala Akademika
Ratnasari, Tri
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Characteristics of DNA Barcodes from Three Thrixspermum Orchids Based on ITS2 Regions Rohimah, Siti; Ratnasari, Tri; Su’udi, Mukhamad
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.23737

Abstract

Thrixspermum (T.) is one of the genus in Orchidaceae that has small flowers. Among species in this genus has a high homology and also has many synonyms. Identification using morphological characters can be constrained since Thrixspermum flowering time occurs in a very short period. This study aimed to conduct molecular-based identification of Thrixspermum orchids using DNA barcoding. This method applied molecular-based species identification technique using DNA sequences from genomic fragments that are considered fast, accurate, and consistent. The molecular markers used were Internal Transcribed Spacer 2 (ITS2), while the samples used were T. centipeda, T. lucidum, and T. angustifolium. BLAST results show that T. centipeda has a close relationship with T. centipeda from Malaysia (KX679332) with 99.79% percent identity, T. lucidum has a high homology with T. linusii (KX679333; 97.30%), while T. angustifolium has a high homology with T. triangulare (KX679348; 99.38%). There is a unique sequence that only T. lucidum and T. angustifolium have that distinguishes the two from other species. ITS2 can be recommended as a molecular marker for determining the Thrixspermum orchid barcode. The benefit obtained from this research is the DNA barcode sequences (ITS2) of Thrixspermum orchids would be very useful to enrich the plant barcodes database for further molecular taxonomy and biodiversity of orchid. 
Pengaruh Cekaman Kekeringan Terhadap Pertumbuhan dan Produksi Tanaman Padi Hitam Varietas Jeliteng Mudhor, Mohammad Ali; Dewanti, Parawita; Handoyo, Tri; Ratnasari, Tri
Agrikultura Vol 33, No 3 (2022): Desember, 2022
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/agrikultura.v33i3.40361

Abstract

Kekeringan merupakan salah satu faktor cekaman abiotik yang dapat menghambat pertumbuhan serta menurunkan hasil tanaman padi. Penanaman varietas tahan kekeringan dengan beberapa perlakuan ketersediaan air diamati pada penelitian ini. Tujuan dari penelitian ini adalah mengkaji karakter morfologi dan fisiologi tanaman padi varietas jeliteng pada berbagai persentase ketersediaan air. Perlakuan yang digunakan dalam penelitian ini yaitu dengan pemberian air optimum padi (kontrol), 100%, 80%, 60%, dan 40% dari ketersediaan air. Hasil penelitian menunjukan bahwa pada kapasitas lapang 80% tanaman padi varietas jeliteng mengalami penurunan hasil yaitu sebesar 36,67% dibandingkan kontrol. Berdasarkan respon fisiologi, pada ketersediaan air 80% terdapat penurunan jumlah klorofil sebesar 10,54% dan peningkatkan jumlah akumulasi kandungan prolin sebesar 21,21%. Berdasarkan respon morfologi, pada 80% ketersediaan air terdapat penurunan kerapatan stomata sebesar 30,85%. Tanaman padi varietas Jeliteng merupakan varietas yang toleran terhadap cekaman kekeringan, hal ini ditunjukkan dengan tidak adanya perbedaan nilai yang signifikan pada berbagai parameter komponen hasil padi yang diamati.
Collaborative Governance dalam Kebijakan Publik melalui Aplikasi Akses Layanan Instan Kota Bogor (ASINAN BOGOR) Studi Kasus: Kecamatan Bogor Timur Kota Bogor Zakiah, Ratu Ulfah; Fadhillah, Dewi Nur; Gustina, Reza; Ratnasari, Tri; Hermawan, Denny; Ramdani, Faisal Tri
Karimah Tauhid Vol. 3 No. 3 (2024): Karimah Tauhid
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/karimahtauhid.v3i3.12423

Abstract

Penelitian ini bertujuan untuk memperoleh pengetahuan tentang penerapan e-Government dalam peningkatan layanan kepada masyarakat, khususnya layanan informasi dan menciptakan pemerintahan yang baik. Pemerintah harus mengikuti kemajuan teknologi agar tidak tertinggal dalam menyediakan pelayanan publik yang baik. Collaborative governance melibatkan pemangku kepentingan diluar pemerintah atau negara secara langsung. Dari sinilah kemudian, Pemerintah Kota Bogor melakukan kemitraan dengan PT. Cartenz menciptakan aplikasi ASINAN Kota Bogor sebagai bentuk pendukung penerapan e-Government khususnya di Kota Bogor. Penelitian ini dilakukan untuk meninjau peran dan kontribusi berbagai pihak dalam proses collaborative governance melalui aplikasi ASINAN Kota Bogor. Studi penelitian ini menggunakan pendekatan kualitatif deskriptif dengan langkah-langkah sistematis studi keperpustakaan atau library research. Tujuan dari pendekatan ini adalah untuk mendeskripsikan dan menganalisis data research. Analisis penelitian collaborative governance dalam kebijakan publik melalui ASINAN Kota Bogor peneliti menggunakan metode yang didasarkan pada konsep Triple Helix. Setiap aktor memiliki peran dalam konsep Triple Helix, yang terdiri dari peran pemerintah, swasta, dan masyarakat. Hasil analisis menunjukan bahwa peran pemerintah, sektor swasta (PT. Cartenz Group), dan masyarakat memiliki peran yang penting dalam kolaborasi ini. Pemerintah bertanggung jawab sebagai regulator dan pemegang kebijakan, sementara PT. Cartenz Group mengembangkan dan menyediakan solusi teknologi, dan masyarakat memberikan masukan dan berperan sebagai pengguna aplikasi. Penelitian ini diharapkan dapat meningkatkan kualitas pelayanan publik, mempercepat penyelesaian permasalahan, dan untuk memaksimalkan pemanfaatan sumber daya secara efisien dan efektif.
Analisis Kualitas Pelayanan Pengujian Kendaraan Angkutan Barang dengan Sistem Bukti Lulus Uji Elektronik Ratnasari, Tri; Salbiah, Euis; Hermawan, Denny
Karimah Tauhid Vol. 3 No. 9 (2024): Karimah Tauhid
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/karimahtauhid.v3i9.15231

Abstract

Dinas Perhubungan (DISHUB) merupakan instansi penyelenggara pelayanan jasa yang bergerak pada bidang transportasi dan perhubungan. Dishub membawahi beberapa unit pelaksana teknis. Salah satu unit pelaksana teknis adalah yang memilik tugas di bidang uji kendaraan, yaitu UPTD PKB. Penggunaan smart card merupakan bentuk produk layanan pengganti buku uji KIR dalam pelayanan uji Kir dengan  sistem Bukti Lulus Uji Elektronik (BLU-E). Penelitian ini bertujuan untuk menganalisis Kualitas Pelayanan Pengujian Kendaraan Angkutan Barang Dengan Sistem BLU-E  Pada Kantor Dishub Kota Depok.
The Effect of Benzyl Amino Purin and NaphtalenaAcetic Acid Applications on Direct Shoot Organogenesis in Porang (Amorphophallus muelleri B) Restanto, Didik Pudji; Aji, Seto Purnomo; Handayani, Etty; Ratnasari, Tri; Jadmiko, Mochammad Wildan; Prayoga, Mohammad Candra; Khozin, Mohammad Nur; Kriswanto, Budi
PLANTA TROPIKA Vol 12, No 1 (2024)
Publisher : Department of Agrotechnology, Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.v12i1.18063

Abstract

Porang (Amorphophallus muelleri B.) is a tuberous plant with the potential to be the main source of carbohydrates and is rich in benefits. Porang proliferation is limited by a 6-month dormancy period per year, and generative propagation is unlikely due to the seeds being apomictic triploid. The aimed of my research to analyze the application of BAP and NAA in culture media for direct propagation of porang shoots. The explant used in this research was young leaves. The research was arranged in a completely randomized design with a combination of BAP and NAA hormones added to the MS medium. There were three BAP treatments, namely 1.0 mg/L, 2.0 mg/L, and 3.0 mg/L, while NAA treatments consisted of 2 levels, namely 2.0 mg/L and 4.0 mg/L. The addition of 1.0 mg/L BAP combined with 4.0 mg/L NAA was the best treatment that produced seven shoots with an average shoot length of 2.14 cm and root length of 3.6 cm, with the earliest bud emergence (9.7 weeks after planting).
Kluwek Seed (Pangium edule Reinw) Germination Response to Soaking Time and Concentration of Gibberellin Acid (GA3) Ratnasari, Tri; Ana, Daniar Alfi; Sulistiyowati, Hari; Setyati, Dwi
Jurnal ILMU DASAR Vol 22 No 2 (2021)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v22i2.9437

Abstract

Kluwek seeds (Pangium edule Reinw.) have a low germination percentage caused by dormancy due to hard seed coat. Kluwek seed germination takes about 1 month. The purpose of this study was to obtain a combination of treatments that were effective in increasing the percentage of kluwek seed germination and reducing the intensity of kluwek seed dormancy. This study used a Completely Randomized Design (CRD) factorial design. The first factor was immersion time (H) consisting of five levels, namely H0: Control (0 hour), H1: 6 hours, H2: 12 hours, H3: 18 hours, and H4: 24 hours and the second factor was giberellin concentration (G) with five levels namely G0: 0 ppm, G1: 25 ppm, G2: 50 ppm, G3: 75 ppm and G4: 100 ppm. Data analysis used Analysis of Variance (Anava) and further tested with Duncans' Multiple Range test (DMRT) at a significant level of 5%. The results obtained showed that the percentage of seed germination without immersion is 6.67%. Soaking using aquades produces an average germination percentage of 28.33%. The most effective treatment was GA3 75 ppm for 24 hours with seed germination of 60%, while the value of dormant intensity is 40%.Keywords: dormancy, germination, gibberellin, kluwek.
Sustaining Biodiversity and Ecological Roles in a Heritage Landscape: The Role of Coffee Agroforestry in Kluncing, Indonesia Kurnianto, Agung Sih; Dewi, Nilasari; Sulistyowati, Hari; Siddiq, Arif Mohammad; Ratnasari, Tri; Khowatini, Husnul; Yulianto, Roni; Firdaus, Anggun Sausan
HAYATI Journal of Biosciences Vol. 32 No. 2 (2025): March 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.2.459-471

Abstract

This study investigates the role of coffee agroforestry in sustaining biodiversity and ecosystem functions in Kluncing, Ijen, Indonesia. By quantifying bird, butterfly, and coffee insect diversity using the Shannon-Wiener index, it evaluates ecological dynamics and their implications for sustainable land management. Bird communities demonstrated the highest diversity (2.911), indicating ecosystem stability, while butterflies (2.481) and coffee insects (1.841) exhibited lower diversity, reflecting habitat and resource limitations. Trophic network modeling using NetworkX identified keystone species like Collocalia linchi (21.9% relative abundance) among birds and the Formicidae family (29.8% relative abundance) among coffee insects, emphasizing their critical roles in ecosystem balance. Canonical Correspondence Analysis (CCA) highlighted the influence of environmental factors on species distribution. Species such as Ariadne ariadne and Delias belisama were sensitive to humidity and temperature, while Collocalia linchi favored areas with higher light intensity. The findings underscore the importance of tailored management practices to address species-specific responses to microclimatic variations. Sustainable agroforestry management is vital for preserving biodiversity, maintaining ecosystem stability, and ensuring agricultural productivity. This study also highlights challenges posed by habitat degradation and climate change, emphasizing the need for adaptive strategies to safeguard this unique agroforestry landscape.
Pemberdayaan Masyarakat Melalui Pembuatan Sabun Berbahan Dasar Minyak Jelantah Sebagai Upaya Mendukung Desa Wonoasri Kecamatan Tempurejo Kabupaten Jember Dalam Menuju Program Kampung Iklim (Proklim) ratnasari, tri; Tri Handoyo; Didik Pudji Restanto; Parawita Dewanti
Jurnal Pengabdian Magister Pendidikan IPA Vol 6 No 4 (2023): Oktober-Desember 2023
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpmpi.v6i4.6302

Abstract

Climate change is a change in the pattern, intensity or shift in the main climate parameters that will have an impact on ecosystems and humans. The impacts of climate change have been felt by humans. To reduce the impacts felt, the government established a climate village program (ProKlim) which combines mitigation and adaptation efforts. Wonoasri Village is one of the buffer villages that borders directly on Meru Betiri National Park. Wonoasri Village has made many efforts to mitigate environmental changes by developing alley forests and yard forests. Apart from that, adaptation efforts have also been carried out by forming joint business groups (KUBE) for community empowerment. Adaptation activities can be carried out by reducing the impact of change by reducing, reusing and recycling waste produced in an area. The joint business group in Wonoasri village includes a creative economy business group that operates in several business objects, one of which is the snack business group. This business group was founded in 2017 and is still actively producing until now. However, there is concern among the village community about this business group because the snack business group produces a lot of used cooking oil waste, almost 20 liters per week. So far, used cooking oil waste is simply thrown away in the yard. Not only from KUBE snacks, used cooking waste that is thrown away without processing also comes from the kitchen activities of village communities. The lack of adaptation efforts for used cooking oil waste produced by KUBE snacks and the Wonoasri village community will certainly pollute the environment. Therefore, the aim of this activity is to develop and encourage environmental adaptation through training in making soap made from used cooking oil as an effort for Wonoasri Village to become one of the ProKlim Villages in Jember Regency. This program is carried out through program outreach activities, workshops on making soap from used cooking oil as well as monitoring and evaluation. Socialization and training activities will be carried out simultaneously on Monday 10 July 2023 from 08.00 to 15.00. The result of the outreach and training is that the people of Wonoasri village know the impact of used cooking waste and can process used cooking waste into soap products. Monitoring will be carried out in August to see the sustainability of the program. KEYWORDS: used cooking soap, proklim, Wonoasri
Pelatihan Aplikasi Zat Pengatur Tumbuh Paclobutrazol pada Lima Varietas Tanaman Padi di Kecamatan Ledokombo, Kabupaten Jember Tanzil, Ahmad Ilham; Fanata, Wahyu Indra Duwi; Sholikhah, Ummi; Ratnasari, Tri; Sugiharto, Bambang; Nikmah, Nasilatun; Raharja, Ajung Gilang Putra; Asshidiqie, Wahyu Pratama; Ramadhani, Rifki Fahrisal; Muhammad, Adibillah; Ariyono, Alief Rizky; Neliana, Intan Ria; Hari Purnomo; Yusuf Rachmandika; Syafina Pusparani; Wahyu Nurkholis Hadi Syahputra
Jurnal Pengabdian Magister Pendidikan IPA Vol 8 No 1 (2025): Januari-Maret 2025
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpmpi.v7i3.8316

Abstract

Tanaman padi yang menghasilkan beras merupakan komoditas utama di Kecamatan Ledokombo. Kendala yang dihadapi petani dalam budidaya termasuk cekaman abiotik berupa angin kencang pada musim penghujan. Hal ini mengakibatkan tanaman padi lokal dengan postur tinggi rentan mengalami rebah. Untuk mengatasi masalah ini, diperlukan pelatihan terkait penggunaan zat pengatur tumbuh guna mengendalikan pertumbuhan tanaman yang tinggi. Paclobutrazol (PBZ), sebagai salah satu jenis zat pengatur tumbuh, berperan penting dalam mengurangi tinggi tanaman. Pelatihan dilakukan langsung di lahan petani dengan menggunakan lima varietas padi yang berbeda. Hasil aplikasi menunjukkan bahwa padi yang ditanam oleh petani menjadi lebih pendek dibandingkan yang tidak diberi perlakuan serupa.
The Effect of Cultivation Media on Matriconditioning Technique and the Concentration of Onion Peel Waste PGR on the Viability and Yield Rice (Oryza sativa) Through the Metabolic Activity of the Seed Amany, Alfiyyah Nur; Setiyono, Setiyono; Sholikhah, Ummi; Ratnasari, Tri; Meliala, Susan Barbara Patricia Sembiring; Arum, Ayu Puspita; Savitri, Dyah Ayu
Journal of Applied Agricultural Science and Technology Vol. 9 No. 2 (2025): Journal of Applied Agricultural Science and Technology
Publisher : Green Engineering Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55043/jaast.v9i2.184

Abstract

A major limiting factor for rice production in the tropics is the decline in seed quality due to storage duration and environmental conditions. Rice seeds are often stored for extended periods, making seed expiration unavoidable. Expired seeds frequently undergo quality deterioration. Therefore, an effective approach is needed to mitigate quality decline and sustain rice production. This study aims to evaluate the effectiveness of different matriconditioning techniques and various concentrations of onion peel waste-derived plant growth regulators (PGRs) in maintaining seed viability and rice yield. The research was conducted using a Completely Randomized Design (CDR) with a two-factor experimental setup and three replicates. The first factor was the matriconditioning medium, consisting of three levels: M1 (soil), M2 (soil + husk charcoal), and M3 (soil + husk ash). The second factor was the concentration of onion peel waste-derived PGR, consisting of four levels: K1 (0% – water), K2 (25% – 250 mL onion peel waste PGR per 1000 mL), K3 (50% – 500 mL onion peel waste PGR per 1000 mL), and K4 (75% – 750 ml onion peel waste PGR per 1000 mL). The results showed that matriconditioning with soil and husk charcoal, along with 25% onion peel waste-derived PGR, enhanced rice seed viability. Additionally, a 75% concentration of onion peel waste PGR significantly influenced the weight of 1000 grains and the total harvested grain weight.
Co-Authors Agung Sih Kurnianto Ahmad Ilham Tanzil Aji, Seto Purnomo Amany, Alfiyyah Nur Ana, Daniar Alfi Arif Mohammad Siddiq Ariyono, Alief Rizky Arum, Ayu Puspita Asshidiqie, Wahyu Pratama Bambang Sugiharto Budi Kriswanto, Budi Cahyono, Haris Denny Hermawan, Denny Dewi, Nilasari Dwi Setyati Dyah Ayu Savitri Euis Salbiah Fadhillah, Dewi Nur Fanata, Wahyu Indra Duwi Firdaus, Anggun Sausan Gustina, Reza Handayani, Etty Hari Purnomo Hari Sulistyowati Hasanah, Ela Apriliyanti Ika Purnamasari Khamidah, Khusna Khowatini, Husnul Khozin, Mohammad Nur Meliala, Susan Barbara Patricia Sembiring Mochammad Wildan Jadmiko Mohammad Ali Mudhor Muhammad, Adibillah Muhtadi, Much. Misbah Neliana, Intan Ria Nikmah, Nasilatun Nisa, Aminatun Novitasari , Adin Nur Widodo Parawita Dewanti Parawita Dewanti Prasojo, Sri Irawan Laras Prayoga, Mohammad Candra Putri, Romadhona Destria Raharja, Ajung Gilang Putra Ramadhani, Rifki Fahrisal Ramdani, Faisal Tri Restanto, Didik Pudji Ristiyana, Suci Setiyono Setiyono Siti Rohimah Soviana, EIida Sri Andriani Su’udi, Mukhamad Sulistiyowati, Hari Syafina Pusparani TRI HANDOYO Tri Handoyo Tri Wahyu Saputra Ummi Sholikhah Utami, Sri Rejeki Wahyu Nurkholis Hadi Syahputra Yulianto, Roni Yusuf Rachmandika Zakiah, Ratu Ulfah