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Yusianti Silviani
SEKOLAH TINGGI ILMU KESEHATAN NASIONAL
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Dentistry Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences

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POTENSI EKSTRAK AQUADES BUNGA KECOMBRANG (Etlingera elatior) SEBAGAI ANTIBAKTERI TERHADAP Escherichia coli DAN Pseudomonas aeruginosa PADA PASIEN INFEKSI SALURAN KEMIH Yusianti Silviani; Dwi Sri Yuwanndari
Klinikal Sains : Jurnal Analis Kesehatan Vol. 13 No. 2 (2025): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v13i2.6842

Abstract

Urinary Tract Infection (UTI) is a common infectious disease, primarily caused by Escherichia coli and Pseudomonas aeruginosa, which are the main UTI-causing bacteria in Indonesia. This study aimed to determine the inhibitory effectiveness of aqueous extract of torch ginger flower (Etlingera elatior) against these bacteria. The extract was obtained through maceration using distilled water as a solvent and tested using the Kirby-Bauer disk diffusion method on bacterial isolates from UTI patient urine samples. Sampling was conducted non-randomly using a quota sampling design with a total of 2 kg of flower sample. The extract concentrations tested were 60%, 70%, 80%, 90%, and 100%, and compared with positive control (ciprofloxacin) and negative control. The results showed that the aqueous extract exhibited inhibitory activity against E. coli and P. aeruginosa, with average inhibition zone diameters ranging from 6–8 mm. Statistical analysis using the Shapiro-Wilk test indicated non-normal data distribution (sig. ≤ 0.001), hence the Kruskal-Wallis test was applied, yielding significance values of 0.003 for E. coli and 0.002 for P. aeruginosa. Further Mann-Whitney test results showed significance values of ≤ 0.05, indicating significant differences between extract concentrations. In conclusion, torch ginger flower extract has potential as a natural antibacterial agent, although its effectiveness is still lower than that of ciprofloxacin.
IDENTIFIKASI Pseudomonas aeruginosa PADA PENGGUNA KAWAT GIGI Viona Syafaattullah; Yusianti Silviani
Klinikal Sains : Jurnal Analis Kesehatan Vol. 14 No. 1 (2026): Juni
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v14i1.7753

Abstract

Dental and oral health problems in Indonesia have a prevalence of 57.6%. This condition is related to the activity of microorganisms in the oral cavity, which can develop due to poor oral hygiene. The use of orthodontic appliances such as braces can increase the risk of plaque buildup and changes in the oral microbiota because the appliance components are difficult to clean. One of the bacteria potentially found in these conditions is Pseudomonas aeruginosa, an opportunistic gram-negative bacterium capable of forming biofilms. The purpose of this study was to identify the presence of Pseudomonas aeruginosa in individuals using fixed orthodontic appliances. This study used a descriptive observational design with a cross-sectional approach. Sampling was carried out by swabbing the orthodontic users' braces using a purposive sampling technique. Inclusion criteria were wearing braces for at least 4 weeks, brushing their teeth at least twice a day, and having no cavities. The samples were then cultured on BHI media, followed by cultivation on MacConkey media, Gram staining, and identification using biochemical tests. The results of a study of 12 samples showed the presence of Pseudomonas aeruginosa bacteria in 25% of the samples. Klebsiella pneumoniae was also found in 50% of the samples, while the remaining samples contained bacteria from the Enterobacteriaceae group. These findings indicate that various types of Gram-negative bacteria can colonize the surface of orthodontic appliances in braces users. Based on the results of the study, it can be concluded that Pseudomonas aeruginosa bacteria were found in 25% of braces users.
IDENTIFIKASI Streptococcus mutans PADA PENGGUNA KAWAT GIGI Rizka Meisya Putri; Yusianti Silviani
Klinikal Sains : Jurnal Analis Kesehatan Vol. 14 No. 1 (2026): Juni
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v14i1.7757

Abstract

The use of fixed orthodontic appliances (braces) contributes to increased plaque and debris accumulation on the tooth surface, which may disrupt the oral microbial balance and promote the proliferation of cariogenic bacteria, including Streptococcus mutans. This study was conducted to identify Streptococcus mutans in individuals wearing braces. The research employed a descriptive observational design with a cross-sectional approach by means of purposive sampling. Samples consisted of swabs from the braces of 14 participants, including 12 users, 1 positive control, and 1 negative control. Bacterial identification was carried out through cultivation in Kaldu Peptone Darah (KPD), isolation on Blood Agar Plates (BAP), gram staining, and biochemical testing. Data were analyzed descriptively. The results showed growth in all samples on KPD, with Streptococcus species dominating at 66.7%. Colony morphology, gram-positive cocci staining, negative catalase test, and carbohydrate fermentation profiles indicated that the isolates belonged to the Streptococcus viridans group. Therefore, it can be concluded that individuals wearing braces harbor Streptococcus species classified within the viridans group.
DISTRIBUSI HASIL TCM TERDUGA TUBERKULOSIS DI UPT PUSKESMAS KRAGILAN BERDASARKAN DURASI MEROKOK DAN NILAI CARDIOTHORATIC RATIO Larasati Puspa Amalia; Yusianti Silviani
Klinikal Sains : Jurnal Analis Kesehatan Vol. 14 No. 1 (2026): Juni
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v14i1.7848

Abstract

Tuberculosis (TB) remains a global health problem, particularly in developing countries such as Indonesia. TB diagnosis can be performed using Rapid Molecular Tests supported by radiological examinations such as the Cardiothoracic Ratio (CTR). Additionally, smoking is a risk factor that can increase susceptibility to TB infection. This study aims to determine the distribution of TCM results in patients suspected of having tuberculosis based on smoking duration and CTR values. This study used a descriptive observational design with a retrospective approach. The study sample consisted of 72 patients suspected of having tuberculosis at the UPT Puskesmas Kragilan who had undergone TCM and X-ray examinations. Data were analyzed descriptively in the form of frequency distributions and percentages. The results showed that 23.6% of patients had positive TCM results and 76.4% had negative results. Based on smoking duration, the passive smokers group had the highest positive percentage (9.7%), followed by >10 years (8.3%) and <10 years (5.6%). Based on CTR values, all positive TCM results were found in the CTR <0.5 group.The conclusion of this study indicates that there is variation in the distribution of TCM results based on smoking duration but does not show a consistent pattern of increase. CTR values do not show a direct association with TCM results.
Co-Authors Abdullah Burhan Yuniarta Afita Indah Puspitasari Alifa Purwahari Putri Ardy Prian Nirwana Didik Wahyudi Dwi Handayani Dwi Sri Yuwanndari Dyah Ayu Novita Sari Endro Dwi Iswanto Farmasiriana Deli Tantias Halwatiah Halwatiah Hari Saktiningsih Hartono Gumilar Julita Niassinta Karisma PuriMahaliya Larasati Puspa Amalia Marcelina Wandan Wisdyafanny Nur Mutianingsih Rizka Meisya Putri Rizky Fadzillah Sungkar Rochmad Hidayah Romadhon Sevy Astriana Sindi Noviana Viona Syafaattullah Yordha Maharani Wahono Putri