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Yusianti Silviani
SEKOLAH TINGGI ILMU KESEHATAN NASIONAL
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences

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POTENSI EKSTRAK AQUADES BUNGA KECOMBRANG (Etlingera elatior) SEBAGAI ANTIBAKTERI TERHADAP Escherichia coli DAN Pseudomonas aeruginosa PADA PASIEN INFEKSI SALURAN KEMIH Yusianti Silviani; Dwi Sri Yuwanndari
Klinikal Sains : Jurnal Analis Kesehatan Vol. 13 No. 2 (2025): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v13i2.6842

Abstract

Urinary Tract Infection (UTI) is a common infectious disease, primarily caused by Escherichia coli and Pseudomonas aeruginosa, which are the main UTI-causing bacteria in Indonesia. This study aimed to determine the inhibitory effectiveness of aqueous extract of torch ginger flower (Etlingera elatior) against these bacteria. The extract was obtained through maceration using distilled water as a solvent and tested using the Kirby-Bauer disk diffusion method on bacterial isolates from UTI patient urine samples. Sampling was conducted non-randomly using a quota sampling design with a total of 2 kg of flower sample. The extract concentrations tested were 60%, 70%, 80%, 90%, and 100%, and compared with positive control (ciprofloxacin) and negative control. The results showed that the aqueous extract exhibited inhibitory activity against E. coli and P. aeruginosa, with average inhibition zone diameters ranging from 6–8 mm. Statistical analysis using the Shapiro-Wilk test indicated non-normal data distribution (sig. ≤ 0.001), hence the Kruskal-Wallis test was applied, yielding significance values of 0.003 for E. coli and 0.002 for P. aeruginosa. Further Mann-Whitney test results showed significance values of ≤ 0.05, indicating significant differences between extract concentrations. In conclusion, torch ginger flower extract has potential as a natural antibacterial agent, although its effectiveness is still lower than that of ciprofloxacin.
IDENTIFIKASI Pseudomonas aeruginosa PADA PENGGUNA KAWAT GIGI Viona Syafaattullah; Yusianti Silviani
Klinikal Sains : Jurnal Analis Kesehatan Vol. 14 No. 1 (2026): Juni
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v14i1.7753

Abstract

Dental and oral health problems in Indonesia have a prevalence of 57.6%. This condition is related to the activity of microorganisms in the oral cavity, which can develop due to poor oral hygiene. The use of orthodontic appliances such as braces can increase the risk of plaque buildup and changes in the oral microbiota because the appliance components are difficult to clean. One of the bacteria potentially found in these conditions is Pseudomonas aeruginosa, an opportunistic gram-negative bacterium capable of forming biofilms. The purpose of this study was to identify the presence of Pseudomonas aeruginosa in individuals using fixed orthodontic appliances. This study used a descriptive observational design with a cross-sectional approach. Sampling was carried out by swabbing the orthodontic users' braces using a purposive sampling technique. Inclusion criteria were wearing braces for at least 4 weeks, brushing their teeth at least twice a day, and having no cavities. The samples were then cultured on BHI media, followed by cultivation on MacConkey media, Gram staining, and identification using biochemical tests. The results of a study of 12 samples showed the presence of Pseudomonas aeruginosa bacteria in 25% of the samples. Klebsiella pneumoniae was also found in 50% of the samples, while the remaining samples contained bacteria from the Enterobacteriaceae group. These findings indicate that various types of Gram-negative bacteria can colonize the surface of orthodontic appliances in braces users. Based on the results of the study, it can be concluded that Pseudomonas aeruginosa bacteria were found in 25% of braces users.
IDENTIFIKASI Streptococcus mutans PADA PENGGUNA KAWAT GIGI Rizka Meisya Putri; Yusianti Silviani
Klinikal Sains : Jurnal Analis Kesehatan Vol. 14 No. 1 (2026): Juni
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v14i1.7757

Abstract

The use of fixed orthodontic appliances (braces) contributes to increased plaque and debris accumulation on the tooth surface, which may disrupt the oral microbial balance and promote the proliferation of cariogenic bacteria, including Streptococcus mutans. This study was conducted to identify Streptococcus mutans in individuals wearing braces. The research employed a descriptive observational design with a cross-sectional approach by means of purposive sampling. Samples consisted of swabs from the braces of 14 participants, including 12 users, 1 positive control, and 1 negative control. Bacterial identification was carried out through cultivation in Kaldu Peptone Darah (KPD), isolation on Blood Agar Plates (BAP), gram staining, and biochemical testing. Data were analyzed descriptively. The results showed growth in all samples on KPD, with Streptococcus species dominating at 66.7%. Colony morphology, gram-positive cocci staining, negative catalase test, and carbohydrate fermentation profiles indicated that the isolates belonged to the Streptococcus viridans group. Therefore, it can be concluded that individuals wearing braces harbor Streptococcus species classified within the viridans group.
DISTRIBUSI HASIL TCM TERDUGA TUBERKULOSIS DI UPT PUSKESMAS KRAGILAN BERDASARKAN DURASI MEROKOK DAN NILAI CARDIOTHORATIC RATIO Larasati Puspa Amalia; Yusianti Silviani
Klinikal Sains : Jurnal Analis Kesehatan Vol. 14 No. 1 (2026): Juni
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v14i1.7848

Abstract

Tuberculosis (TB) remains a global health problem, particularly in developing countries such as Indonesia. TB diagnosis can be performed using Rapid Molecular Tests supported by radiological examinations such as the Cardiothoracic Ratio (CTR). Additionally, smoking is a risk factor that can increase susceptibility to TB infection. This study aims to determine the distribution of TCM results in patients suspected of having tuberculosis based on smoking duration and CTR values. This study used a descriptive observational design with a retrospective approach. The study sample consisted of 72 patients suspected of having tuberculosis at the UPT Puskesmas Kragilan who had undergone TCM and X-ray examinations. Data were analyzed descriptively in the form of frequency distributions and percentages. The results showed that 23.6% of patients had positive TCM results and 76.4% had negative results. Based on smoking duration, the passive smokers group had the highest positive percentage (9.7%), followed by >10 years (8.3%) and <10 years (5.6%). Based on CTR values, all positive TCM results were found in the CTR <0.5 group.The conclusion of this study indicates that there is variation in the distribution of TCM results based on smoking duration but does not show a consistent pattern of increase. CTR values do not show a direct association with TCM results.
Efektivitas Penyuluhan Kesehatan dalam Meningkatkan Pengetahuan Masyarakat tentang Risiko dan Pencegahan Leptospirosis Ardy Prian Nirwana; Yusianti Silviani; Didik Wahyudi
Abdimas Mandalika Vol 5, No 4 (2026): Juni
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/am.v5i4.39517

Abstract

Abstract:  Leptospirosis is a zoonotic disease that remains a significant public health problem, particularly in areas with poor environmental sanitation and high-risk community activities. This community service program aimed to improve public knowledge and awareness regarding the risks and prevention of leptospirosis in Sobayan Village, Pedan District, Klaten Regency. The methods employed included interactive health education sessions, basic health screenings (blood glucose and blood pressure), and training on the preparation of natural antiseptic soap as part of promotive and preventive efforts. Program evaluation was conducted using a pre-test and post-test design to assess changes in participants’ knowledge levels. The results demonstrated a significant increase in community knowledge, as indicated by the rise in the average score from 71.21 in the pre-test to 91.89 in the post-test. In addition, the program contributed to enhancing community awareness in adopting clean and healthy living behaviors (PHBS). In conclusion, this community service activity was effective in improving knowledge and promoting behavioral change as part of leptospirosis prevention efforts.Abstrak: Leptospirosis merupakan penyakit zoonosis yang masih menjadi masalah kesehatan masyarakat, terutama pada wilayah dengan sanitasi lingkungan yang kurang memadai dan aktivitas masyarakat yang berisiko tinggi. Kegiatan pengabdian kepada masyarakat ini bertujuan untuk meningkatkan pengetahuan dan kesadaran masyarakat mengenai bahaya serta upaya pencegahan leptospirosis di Desa Sobayan, Kecamatan Pedan, Kabupaten Klaten. Metode pelaksanaan meliputi penyuluhan kesehatan interaktif, pemeriksaan kesehatan dasar berupa skrining kadar gula darah dan tekanan darah, serta edukasi pembuatan sabun antiseptik alami sebagai upaya promotif dan preventif. Evaluasi dilakukan menggunakan desain pre-test dan post-test untuk mengukur perubahan tingkat pengetahuan peserta. Hasil kegiatan menunjukkan adanya peningkatan pengetahuan yang signifikan, ditunjukkan oleh kenaikan nilai rata-rata dari 71,21 pada pre-test menjadi 91,89 pada post-test. Selain itu, kegiatan ini juga berkontribusi dalam meningkatkan kesadaran masyarakat terhadap penerapan perilaku hidup bersih dan sehat (PHBS). Dengan demikian, kegiatan pengabdian ini efektif dalam meningkatkan pengetahuan dan mendorong perubahan perilaku masyarakat dalam upaya pencegahan leptospirosis
Distribution and Selected Beta-Lactam Susceptibility of Escherichia coli and Staphylococcus aureus in Suspected Sepsis Patients at RSUD Ir. Soekarno Yusianti Silviani; Mustofa Tri Wijayanto
Jurnal Biologi Tropis Vol. 26 No. 3 (2026): Juli - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i3.12055

Abstract

Sepsis is a life-threatening organ dysfunction caused by a dysregulated host response to infection, and antimicrobial resistance may compromise empirical therapy. This retrospective descriptive study described the distribution of Escherichia coli and Staphylococcus aureus and selected beta-lactam susceptibility patterns among patients with suspected sepsis at RSUD Ir. Soekarno Sukoharjo during 2024–2025. Secondary data from blood, urine, sputum, and pus cultures, including antimicrobial susceptibility testing using the VITEK 2 Compact system, were analyzed descriptively as counts and proportions. Escherichia coli accounted for five isolates in 2024 and four in 2025, whereas Staphylococcus aureus was represented by one isolate in 2024 and was not identified in 2025. Resistance to ceftazidime was observed in 3/5 (60.0%) Escherichia coli isolates in 2024 and 3/4 (75.0%) in 2025, while ceftriaxone resistance occurred in 4/5 (80.0%) and 3/4 (75.0%), respectively. The single Staphylococcus aureus isolate was resistant to oxacillin and phenotypically consistent with MRSA. These findings indicate frequent resistance to third-generation cephalosporins among a small number of Escherichia coli isolates. Interpretation should be cautious because of the limited number of isolates and the absence of confirmatory ESBL or MRSA testing data.
Co-Authors Abdullah Burhan Yuniarta Afita Indah Puspitasari Alifa Purwahari Putri Ardy Prian Nirwana Didik Wahyudi Dwi Handayani Dwi Sri Yuwanndari Dyah Ayu Novita Sari Endro Dwi Iswanto Farmasiriana Deli Tantias Halwatiah Halwatiah Hari Saktiningsih Hartono Gumilar Julita Niassinta Karisma PuriMahaliya Larasati Puspa Amalia Marcelina Wandan Wisdyafanny Mustofa Tri Wijayanto Nur Mutianingsih Rizka Meisya Putri Rizky Fadzillah Sungkar Rochmad Hidayah Romadhon Sevy Astriana Sindi Noviana Viona Syafaattullah Yordha Maharani Wahono Putri