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AKTIVITAS PENGHAMBATAN BAKTERI PEMBENTUK HISTAMIN DAN ANTIOKSIDAN KAPANG ENDOFIT KUNYIT YANG BERPOTENSI SEBAGAI PENGAWET ALAMI Septiana, Eris; Simanjuntak, Partomuan
Biopropal Industri Vol 7, No 1 (2016)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (576.513 KB)

Abstract

Microorganisms activity and oxidation process can contribute to food spoilage occurrence. Natural source that have antimicrobial activity and antioxidant are potential as a natural preservative. The aims of this study are to investigate the endophytic fungi from turmeric plant which have antibacterial toward histamine-producing bacteria and antioxidant activity. Antibacterial activity of histamine-producing bacteria was done by paper disc diffusion, whereas the antioxidant activity was performed by free radical scavenging method. In the paper disc diffusion method were obtained 3 isolates which have the highest diameter of inhibition at concentration of 20.000 ppm: from root (15 mm), flower (14 mm) and  rhizome (13 mm). Also at the antioxidant assay, the lowest IC50 values were isolates from root (39,52 ppm), flower (157,93 ppm) and rhizome (96 ppm). Endophytic fungi from root, flower and rhizome had antimicrobial and antioxidant activity thus they could potentially used as a natural food preservative.Keywords: antibacterial, antioxidant, endophyte, preservative,  turmericABSTRAKAktivitas mikroorganisme dan proses oksidasi dapat menyebabkan kerusakan pangan. Bahan alam yang mempunyai aktivitas sebagai antimikroba dan antioksidan berpotensi sebagai bahan pengawet alami. Penelitian ini bertujuan untuk mengetahui kapang endofit asal tanaman kunyit yang memiliki aktivitas antibakteri penghasil histamin dan antioksidan. Aktivitas antibakteri penghasil histamin dilakukan dengan menggunakan metode difusi cakram kertas, sedangkan aktivitas antioksidan dilakukan dengan metode peredaman radikal bebas. Pada metode difusi cakram kertas didapatkan 3 isolat yang memiliki diameter daya hambat tertinggi sampai konsentrasi 20.000 ppm yaitu berasal dari akar (15 mm), bunga (14 mm) dan rimpang (13 mm). Pada uji antioksidan didapatkan nilai IC50 terendah yaitu isolat yang berasal dari akar (39,52 ppm), bunga (157,93 ppm) dan rimpang (96 ppm). Isolat kapang endofit yang berasal dari akar, bunga dan rimpang memiliki aktivitas antimikroba dan antioksidan sehingga berpotensi digunakan sebagai bahan pengawet makanan alami.Kata kunci: antibakteri, antioksidan, endofit, kunyit, pengawet
Toksisitas dan Aktivitas Antimalaria Melalui Penghambatan Polimerisasi Hem Secara In Vitro Ekstrak Daun Sambiloto (Andrographis paniculata) Septiana, Eris; Gianny, Demitra; Simanjuntak, Partomuan
Media Penelitian dan Pengembangan Kesehatan Vol 27, No 4 (2017)
Publisher : Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/mpk.v27i4.6499.255-262

Abstract

Sambiloto (Andrographis paniculata) has been used to treat malaria. In vitro research using parasite or through heme polymerization inhibition using andrografolide and in vivo using infected animal test have been done widely, however, heme polymerization inhibition from extracts with different polarity levels has not been studied yet. The aims of this study were to investigate the heme polymerization inhibition activity and toxicity of sambiloto leaf extracts. Sambiloto leaf extracted with gradually maceration using n-hexane, ethyl acetate, and 70% ethanol respectively. Heme polymerization inhibition activity was used as in vitro antimalarial test. Brine shrimp lethality test (BSLT) was used to determine toxicity of the extracts. Phytochemically screening was done for all extracts qualitatively. The results of this study were n-hexane, ethyl acetate, and 70% ethanol extracts had heme polymerization inhibition activities with IC50 values at 2,196.57; 1,235.54; and 1,157.24 μg/mL respectively. N-hexane, ethyl acetate, and 70% ethanol have LC50 values at 1,155.79; 1,133.89; and 5,229.15 μg/mL respectively. 70% ethanol extract contains alkaloid, flavonoid, steroid/triterpenoid, saponin, and tannin. N-hexane and ethyl acetate extracts has only contains alkaloid and flavonoid. 70% ethanol, ethyl acetate, and n-hexane extracts of sambiloto leaf have ability to inhibit heme polymerization and also non toxic to Artemia salina larvae.   Abstrak Sambiloto (Andrographis paniculata) telah lama digunakan untuk mengobati malaria. Penelitian in vitro dengan parasit maupun melalui penghambatan polimerisasi hem dengan senyawa andrografolida dan in vivo dengan hewan uji terinfeksi telah dilakukan, namun demikian belum ada penelitian tentang penghambatan polimerisasi hem ekstrak dengan tingkat kepolaran yang berbeda. Penelitian ini bertujuan untuk mengetahui aktivitas penghambatan polimerisasi hem dan toksisitas ekstrak daun sambiloto. Daun sambiloto diekstraksi secara maserasi bertingkat berturut-turut menggunakan pelarut n-heksan, etil asetat, dan etanol 70%. Uji antimalaria secara in vitro menggunakan metode penghambatan polimerisasi hem. Uji toksisitas menggunakan metode uji kematian larva Artemia salina (BSLT). Skrining fitokimia dilakukan secara kualitatif untuk seluruh ekstrak. Hasil penelitian menunjukkan bahwa ekstrak n-heksan, etil asetat, dan etanol 70% memiliki aktivitas penghambatan polimerisasi hem dengan nilai IC50 masing-masing sebesar 2.196,57; 1.235,54; dan 1.157,24 μg/mL. Ekstrak n-heksan, etil asetat, dan etanol 70% masing-masing memiliki nilai LC50 sebesar 1.155,79; 1.133,89; dan 5.229,15 μg/mL. Ekstrak etanol 70% mengandung alkaloid, flavonoid, steroid/triterpenoid, saponin, dan tannin. Ekstrak n-heksan dan etil asetat hanya mengandung alkaloid dan steroid/triterpenoid. Ekstrak etanol 70%, etil asetat, dan n-heksan daun sambiloto memiliki kemampuan dalam menghambat polimerisasi hem dan tidak toksik terhadap larva Artemia salina.
ISOLASI DAN IDENTIFIKASI KAPANG ENDOFIT ASAL AKAR TANAMAN KUNYIT (Curcuma longa) SEBAGAI ANTIMALARIA Septiana, Eris; Rachman, Fauzy; Lekatompessy, Sylvia J.R.; Sukiman, Harmastini I.; Simanjuntak, Partomuan
BERITA BIOLOGI Vol 17, No 3 (2018)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5157.146 KB) | DOI: 10.14203/beritabiologi.v17i3.3408

Abstract

Malaria is still the leading cause of death worldwide with nearly half the worlds population at risk. Parasitic resistance to existing antimalarial drugs in the market makes the search for a source of new drugs from nature is very important. Therefore, the aims of this study are to determine in vitro antimalarial activity of endophytic fungi extract from turmeric root and to identify the selected isolate molecularly. Heme polymerization inhibition method was used as in vitro antimalarial assay. The selected isolate was thrn identified using ITS1, ITS2, and 5.8S sequences of rDNA. The result of this study obtained 16 isolates of endophytic fungi from root of turmeric plant with isolate code were of K.Cl.Sb.A1 - K.Cl.Sb.A16. All of the ethyl acetate extracts of isolated endophytic fungi have heme polymerization inhibition activity. K.Cl.Sb.A11 was the most active isolate on heme polymerization inhibition test with 94,31% at concentration of test material at 8 mg/mL and IC50 value at 1.84 mg/mL. Molecular analysis showed that K.Cl.Sb.A11 isolate was Penicillium sp. and potentially developed as an antimalarial drug.
AKTIVITAS ANTIMIKROBA DAN ANTIOKSIDAN EKSTRAK BEBERAPA BAGIAN TANAMAN KUNYIT (Curcuma longa) Septiana, Eris; Simanjuntak, Partomuan
FITOFARMAKA | Jurnal Ilmiah Farmasi Vol 5, No 1 (2015): FITOFARMAKA
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (223.447 KB)

Abstract

Kunyit (Curcuma longa) merupakan tanaman obat tradisional yang biasa digunakan sebagai bumbu masakan dan sebagai bahan obat meliputi antimikroba, antioksidan,antitumor, dan anti inflamasi. Tujuan dari penelitian ini ialah untuk mengetahui aktivitas antimikroba dan antioksidan dari beberapa organ tanaman kunyit meliputi akar, rimpang, batang, dan daun. Semua bagian diekstraksi dengan etanol dan etil asetat. Seluruh ekstrak etanol dan etil asetat diuji aktivitas antimikrobanya menggunakan metode difusi cakram kertas terhadap Escherichia coli,Staphylococcus aureus, dan Candida albicans. Kloramfenikol dan nistatin masing-masing digunakan sebagai kontrol positif untuk uji antibakteri dan antijamur, sedangkan masing-masing pelarut untuk ekstraksi juga digunakan sebagai kontrol negatif. Aktivitas antioksidan dilakukan menggunakan metode 1,1-difenil-2pikril hidrazil (DPPH) dan asam askorbat digunakan sebagai standar. Hasil aktivitas antimikroba menunjukkan bahwa ekstrak etil asetat dari daun dan batang memiliki aktivitas penghambatan tertinggi terhadap S. aureus, ekstrak etil asetat dari akar dan batang memiliki aktivitas penghambatan tertinggi terhadap E. coli, dan ekstrak etil asetat dari daun memilikiaktivitas penghambatan tertinggi terhadap C. albicans. Ekstrak etil asetat dari rimpangmemiliki aktivitas antioksidan tertinggi diantara ekstrak lainnya.
PENGHAMBATAN AKTIVITAS ENZIM α-GLUKOSIDASE DARI EKSTRAK AIR TANAMAN RARU (Vatica pauciflora) SECARA IN VITRO septiana, eris; winarti, wiwi; simanjuntak, partomuan
Farmasains : Jurnal Ilmiah Ilmu Kefarmasian Vol 4 No 1 (2017)
Publisher : Universitas Muhammadiyah Prof. DR. HAMKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (558.706 KB) | DOI: 10.22236/farmasains.v4i1.96

Abstract

Diabetes mellitus is a metabolic disorder that leading the blood sugar levels become higher than normal. In Indonesia, one of the plants that traditionally used to cure hyperglycemic is raru plant (Vatica pauciflora). The objectives of this research was to investigate α-glucosidase inhibitory activity from stem bark, root, and leaf of raru plant. Extraction of stem bark, root, and leaf was conducted with reflux at 70 °C by using water as a solvent. In vitro anti-diabetic activity was tested using the method of α-glucosidase inhibition. The results show that water extracts of raru’s stem bark, root, and leaf have α-glucosidase inhibitory activity. The stem bark water extract has the highest activity than leaf and root with IC50 values were 13.53, 16.96, and 41.91 ppm respectively. All extracts is categorized as active in inhibiting the activity of α-glucosidase enzyme by having IC50≤100 ppm. The results obtain in this research clearly indicate a promising potential as anti-diabetic properties of raru plant.
Antioxidant Activity of Endophytic Fungi Isolated from Turmeric Flowers Septiana, Eris; Yadi, Yadi; Simanjuntak, Partomuan
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 2 (2020): August 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i2.24396

Abstract

Antioxidants are compounds needed by the body to fight free radicals that can cause adverse effects on health. All parts of turmeric plant have antioxidant activity, so does the flower. It is thought that the endophytic fungi from turmeric flowers also have antioxidant activity and no studies have reported it yet. The purpose of this study was to study the antioxidant activity of single and mixed cultures of endophytic fungi isolated from turmeric flowers. In vitro antioxidant activity assay was conducted using the DDPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging method by measuring its absorption using a UV-Vis spectrophotometer at a wavelength of 517 nm. The extract of single and mixed cultures of endophytic fungi isolated from turmeric flowers showed antioxidant activity. The combination of fungal cultures can increase the antioxidant activity of a single culture. This study is the first to report that the combination of endophytic fungi from turmeric flowers can increase its antioxidant activity. Therefore, the presence of the endophytic fungi in turmeric flowers that have antioxidant activity can provide additional information so that the utilization of turmeric flowers as a source of natural antioxidants will increase.
Potensi Ekstrak Kapang Endofit Asal Rimpang Kunyit sebagai Antimalaria dan Antioksidan Septiana, Eris; bustanussalam, Bustanussalam; Rachman, Fauzy; Simanjuntak, Partomuan
Jurnal Kefarmasian Indonesia VOLUME 7, NOMOR 1, FEBRUARI 2017
Publisher : Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jki.v7i1.3482

Abstract

Malaria infection is still a public health problem, especially in developing countries, including Indonesia. Malaria infection is usually accompanied by increased free radicals in the body of the patient. This situation will cause decreasing the immune system of the patient. Explorating of new drugs that have antimalarial and also antioxidant activity is very important. Therefore, this study aims to determine in vitro antimalarial and antioxidant activity of endophytic fungi extract origin turmeric from Sukabumi. This study begins with fermentation process of endophytic fungi on the broth medium and ethyl acetate was used to extract the filtrat to gain the test extracts. Heme polymerization inhibition and free radical DPPH scavenging method were used to antimalarial and antioxidant in vitro assay respectively. The results showed that 18 isolates of endophytic fungi have antimalarial activity and one isolate inactive and also all isolates have antioxidant activity. Smi.Cl.6F isolate was the most active isolate on the antimalarial and antioxidant assay with IC50 value of each assay were 1.93 mg/mL and 32.28 mg/L respectively. Therefore, Smi.Cl.6F isolates potentially be used as new antimalarial drugs
Pengaruh Variasi Media Pertumbuhan terhadap Aktivitas Peredaman Radikal Bebas DPPH Ekstrak Kapang Endofit Isolat Cb.D1 Purnamasari, Ayu; Andriyaningsih, Fitri; Pamungkas, Riska Andriani; Septiana, Eris
Jurnal Kefarmasian Indonesia VOLUME 12, NOMOR 2, AGUSTUS 2022
Publisher : Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jki.v0i0.6029

Abstract

Endophytic fungi are a source of antioxidant compounds in nature. The low yield of extracts and active compounds produced is one of the limiting factors for using endophytic fungi as a source of natural antioxidant compounds. Modification of growth media is an alternative solution to overcome this problem. This study aimed to determine the effect of media conditions, belonging to carbon and nitrogen sources and different initial pH of fermentation, on the antioxidant through DPPH radical scavenging activity of extract of endophytic fungi Cb.D1 isolated from cinnamon plant leaves. The culture was propagated using Czapek Dox Broth basal liquid medium with agitation speed 120 rpm at room temperature for 14 days. The carbon sources used were glucose, sucrose, and soluble starch. The nitrogen sources were natrium nitrate, ammonium nitrate, and yeast extract. The initial pH conditions used were 5, 7, and 9, and ethyl acetate as the extraction solvent. The results obtained that the variation of nitrogen and carbon sources and also initial pH conditions can increase the yield of extract of endophytic fungi Cb.D1. Glucose, yeast extract, and initial pH at 9 were the best growth media to gain it. The substitution of nitrogen sources and initial pH can increase the DPPH free radical scavenging activity of endophytic fungal extract compared to substitution for carbon sources. The highest activity from the Cb.D1 endophytic fungal extract was obtained from media that contain sucrose and natrium nitrate as a carbon and nitrogen sources and an initial pH of 5. The Variation of growth media of endophytic fungi Cb.D1 affected their extract in DPPH free radical scavenging activity.
Aktivitas Antioksidan Kapang Endofit asal Rumput Laut Caulerpa lentilifera Septiana, Eris; Hardhiyuna, Mutia; Untari, Febriana; Bustanussalam, Bustanussalam; Rahmawati, Siti Irma
Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 20, No 1 (2025): Juni 2025
Publisher : Politeknik - Ahli Usaha Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jpbkp.v20i1.1040

Abstract

Aktivitas antioksidan dari ekstrak rumput laut telah banyak dilaporkan. Namun, belum banyak peneliti yang melaporkan aktivitas antioksidan dari kapang endofit asal rumput laut. Penelitian ini bertujuan untuk mengisolasi kapang endofit dari rumput laut C. lentilifera, mengevaluasi aktivitas antioksidan, serta profil senyawa kimia ekstrak terpilih. Isolasi kapang endofit dilakukan dengan metode sterilisasi permukaan dan ditanam pada media Potato Dextrose Agar (PDA). Uji antioksidan menggunakan metode 2,2-Diphenyl-1-picrylhydrazyl (DPPH), 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS), dan ferric reducing antioxidant power (FRAP). Penentuan kadar fenolik dan flavonoid total masing-masing berdasarkan pada reaksi reagen Follin-Ciocalteu dan aluminium klorida. Ekstrak dengan aktivitas antioksidan terbaik dilakukan profiling senyawa kimianya menggunakan Kromatografi Gas-Spektroskopi Massa (KG-SM). Hasil isolasi didapatkan lima isolat kapang endofit yaitu Cl.Tka.S1, Cl.Tka.S2, Cl.Tka.S3, Cl.Tka.R1, dan Cl.Tka.R2. Ekstrak kapang endofit Cl.Tka.S3 memiliki aktivitas tertinggi dengan nilai IC50 sebesar 17,65 ± 1,88 dan 37,07 ± 2,16 mg/L pada metode ABTS dan DPPH,serta uji FRAP dengan kadar setara Fe(II) sebesar 1654,15 ± 32,95 µmol/mg ekstrak. Kadar fenolik total tertinggi didapatkan dari ekstrak kapang endofit Cl.Tka.S1 sebesar 75,05 ± 8,46 mg setara asam galat/g ekstrak. Kadar flavonoid total tertinggi didapatkan dari ekstrak kapang endofit Cl.Tka.S3 sebesar 38,50 ± 1,55 mg setara kuersetin/g ekstrak. Profiling senyawa kimia menunjukkan ekstrak isolat Cl.Tka.3 sebagai isolat terbaik mengandung senyawa antioksidan berupa senyawa golongan fenolik, asam lemak, alkana, dan alkohol. KATA KUNCI: ABSTRACTThe antioxidant activity of seaweed extracts has been widely reported. However, reports on the antioxidant activity of endophytic fungi derived from seaweed are still limited. This study aims to isolate endophytic fungi from C. lentilifera, determine antioxidant activity and chemical profiles of selected extracts. Endophytic fungi were isolated by the surface sterilization method and planted on Potato Dextrose Agar (PDA) media. Antioxidant test using 2,2-Diphenyl-1-picrylhydrazyl (DPPH), 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS), dan ferric reducing antioxidant power (FRAP) methods. Total phenolic and flavonoid content was based on the reaction of Follin-Ciocalteu reagent and aluminium chloride, respectively. Extracts with the best antioxidant activity were profiled for their chemical compounds using Gas Chromatography-Mass Spectroscopy (GC-MS). The isolation results obtained five isolates of endophytic fungi, namely Cl.Tka.S1, Cl.Tka.S2, Cl.Tka.S3, Cl.Tka.R1, and Cl.Tka.R2. Cl.Tka.S3 extract had the highest activities with an IC50 value of 17.65 ± 1.88 and 37.07 ± 2.16 mg/L on the ABTS and DPPH methods, as well as FRAP test values with Fe(II) equivalent levels of 1654.15 ± 32.95 µmol/mg extract. Cl.Tka.S1 extract has the highest total phenolic content of 75.05 ± 8.46 mg gallic acid equivalent/g extract. Cl.Tka.S3 extract has the highest total flavonoid content of 38.50 ± 1.55 mg quercetin equivalent/g extract. Profiling of chemical compounds showed that the Cl.Tka.S3, as the best extract, contained antioxidant compounds such as phenolic compounds, fatty acids, alkane, and alcohol.
AKTIVITAS ANTIOKSIDAN KOMBINASI EKSTRAK ETANOL DAUN JARONG (Stachytarpheta indica) DAN BATANG CENTE (Lantana camara) Septiana, Eris; Mawadah, Nursifa; Simanjuntak, Partomuan
Media Farmasi: Jurnal Ilmu Farmasi Vol. 17 No. 2: September 2020
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/mf.v17i2.17299

Abstract

Penggunaan tanaman obat sebagai sumber senyawa antioksidan semakin meningkat. Beberapa tanaman yang telah dilaporkan sebagai sumber senyawa antioksidan adalah daun jarong (Stachytarpheta indica) dan batang cente (Lantana camara). Namun, belum ada penelitian yang melaporkan kombinasi kedua tanaman tersebut sebagai sumber antioksidan. Oleh karena itu, penelitian ini bertujuan untuk mengetahui pengaruh kombinasi ekstrak etanol daun jarong dan batang cente terhadap aktivitas antioksidannya. Aktivitas antioksidan in vitro berdasarkan pada metode peredaman radikal bebas DPPH (2,2-diphenyl-1-picrylhydrazyl). Penapisan fitokimia dan analisis indeks kombinasi juga dilakukan untuk menentukan efek kombinasinya. Semua ekstrak etanol tunggal dan kombinasi menunjukkan aktivitas antioksidan. Kombinasi ekstrak etanol daun jarong dan batang cente dalam perbandingan 1:2 memberikan aktivitas antioksidan tertinggi dengan nilai IC50 sebesar 18,86 µg/mL. Penapisan fitokimia menunjukkan bahwa senyawa steroid, tanin, flavonoid, dan fenolik ditemukan pada kedua ekstrak. Alkaloid, kuinon, dan saponin hanya ditemukan dalam ekstrak daun jarong. Sementara itu, kumarin hanya ditemukan dalam ekstrak batang cente. Kombinasi ekstrak etanol menunjukkan efek sinergis ringan-sedang sampai mendekati aditif dengan nilai CI antara 0,7723-0,9607. Oleh karena itu, kombinasi ekstrak dapat meningkatkan aktivitas antioksidannya.