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All Journal Agrosains: Jurnal Penelitian Agronomi Caraka Tani: Journal of Sustainable Agriculture
Nandariyah Nandariyah
Department of Agrotechnology, Faculty of Agriculture, Universitas Sebelas Maret, Surakarta
Agriculture, Biological Sciences & Forestry

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Selection for Growth Traits on M1V1 Generation of Raja Bulu Banana (Musa paradisiaca Linn.) Obtained by Gamma Rays Irradiation Nandariyah Nandariyah; Endang Yuniastuti; Sukaya Sukaya; Sonia Ika Yudhita
Caraka Tani: Journal of Sustainable Agriculture Vol 36, No 1 (2021): April
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/carakatani.v36i1.34492

Abstract

Raja Bulu is one of the banana varieties favored by the community because of its thick fruit flesh and sweet taste. However, its parthenocarpic characteristic and vegetative propagation make this banana variety has limited genetic variation. Attempt to improve the genetic variation was conducted through induced mutation breeding using gamma-ray mutagens. This research aimed to select M1V1 generation of Raja Bulu banana (Musa paradisiaca Linn.) obtained by gamma rays’ irradiation for their growth traits which are expected to produce banana varieties that have an early maturity and high yield. This study used a randomized complete block design without replication by observing the generative growth of each individual of Raja Bulu banana irradiated by gamma rays and without radiation as a control. The results showed that gamma-ray irradiation treatment caused Raja Bulu banana to be harvested earlier and produced higher fruit weight than controls. The gamma-ray irradiation had a random influence on Raja Bulu bananas. The 10 Gy gamma-ray irradiation dosage influenced the morphological diversity in the generative phase of Raja Bulu banana. The treatment of gamma irradiation resulted in 5 individual plants that flowered and matured earlier as compared to controls.
Study of Black Rice Parents Performance and the Crossing Ability Nandariyah Nandariyah; Sukaya Sukaya; Djoko Purnomo; Sutarno Sutarno; Endang Yuniastuti; Catalina Dara Ayu Az-Zahra
Caraka Tani: Journal of Sustainable Agriculture Vol 38, No 1 (2023): April
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/carakatani.v38i1.60245

Abstract

Black rice generally has problems in cultivation such as relatively long plant life, high habitus and low productivity. Plant breeders use several methods in hybridization activities, such as backcross method that can lead to the development towards emphasizing the superiority of each parent. This research aimed to study and determined the success rate of the black rice lines F1 backcross and the parents’ performance. This research was conducted using F1 of promising lines, parental lines and the Jeliteng variety with 9 sets of crosses. Each line and variety were repeated 3 times, in total there were 57 experimental units. The observation parameters of this research were plant height, crossing success, weight of seeds, length and width of grain, flowering age, harvesting age also number of grains and unfilled spikelet. The results of this study showed that parents plant height was positively correlated with the number of productive tillers. The taller the plant, the more productive tillers and the more flowers can be crossed. The success of the cross can increase with the number of flowers crossed. The success of crosses between F1 black rice promising lines and their parents has a success rate range of 10.82% to 33.75%. The findings imply that F1 crossbreeding of black rice promising lines with their parents can be carried out to produce backcross offspring.
Keragaman Genetik Sirih Cina (Peperomia pellucida L.) Berdasarkan Penanda RAPD (Random Amplified Polymorphic DNA) Nandariyah Nandariyah; Hardian Ningsih; Annisa Nur Fadhillah; Parjanto Parjanto; Edi Paryanto
Agrosains : Jurnal Penelitian Agronomi Vol 26, No 1 (2024): Agrosains: Jurnal Penelitian Agronomi
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/agsjpa.v26i1.92694

Abstract

Chinese betel is a plant widely found in Indonesia, especially in the ex-Karasidenan area of Surakarta. The existence of growing Chinese betel can be found at various altitudes with humid growing conditions with low sun intensity. People in Surakarta believe that Chinese betel can be used as a traditional medicine. Chinese betel nut is suspected to contain a minerals and bioactive substances that are beneficial to health. Currently, there are few references to Chinese betel, especially related to genetic diversity. This study aims to study the diversity of Chinese betel band patterns based on RAPD markers using OPA-3, OPA-4, OPA-11, OPC-05, and OPD-4 primers. This research was carried out at the Plant Breeding Laboratory, Faculty of Agriculture, Gadjah Mada University, Yogyakarta in July - November 2023 using Chinese betel leaf DNA taken from various places in the ex-karasidenan area of Surakarta. The results of the data analyzed by NTSYSpc version 2.02 with the SimQual (Similarity for Qualitative Data) function using the UPGMA (Unweighted Pair Group with Arithmetic Averaging) method based on the Jaccard similarity index value. The amplification results showed 30 bands with 13 polymorphic bands and 17 monomorphic bands. The level of polymorphism among Chinese betel samples in the five RAPD primers used presented 44.36% of the total 13 polymorphic bands. Jaccard's genetic similarity matrix data ranged from 0.667 – 0.964. The results of the dendogram show that the sample is divided into two clusters with a diversity index of 0.28.
Co-Authors Annisa Nur Fadhillah Catalina Dara Ayu Az-Zahra Djoko Purnomo Edi Paryanto Endang Yuniastuti Hardian Ningsih Parjanto Parjanto Sonia Ika Yudhita Sukaya Sukaya Sutarno Sutarno