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Korelasi dan Analisis Lintas Beberapa Karakter Tanaman Cabai (Capsicum annuum L.) pada Kondisi Normal dan Tercekam Kekeringan (Correlations and Path Analysis of Some Characters in Chili Pepper (Capsicum annuum L.) Under Normal and Drought Stress) nFN - Rosmaina; nFN Sobir; nFN Parjanto; Ahmad Yunus
Jurnal Hortikultura Vol 29, No 2 (2019): Desember 2019
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v29n2.2019.p147-158

Cekaman air merupakan salah satu faktor pembatas pertumbuhan dan produksi tanaman termasuk cabai (Capsicum annuum L.). Produktivitas tanaman merupakan karakter yang kompleks sehingga hubungan antarkarakter perlu diketahui untuk mendapatkan kriteria seleksi yang tepat untuk perbaikan tanaman. Penelitian ini bertujuan mengetahui kriteria seleksi terhadap produksi tanaman cabai toleran kekeringan pada fase pembungaan. Penelitian disusun menggunakan Rancangan Acak Lengkap dengan tiga taraf cekaman air, yaitu 100% kapasitas lapang (kontrol), 50% kapasitas lapang (medium stress), dan 25% kapasitas lapang (extreme stress). Hasil analisis korelasi menunjukkan bahwa berbagai karakter yang diamati memperlihatkan perbedaan asosiasi dengan level cekaman kekeringan. Karakter panjang akar tidak dapat digunakan sebagai kriteria seleksi tanaman cabai yang toleran terhadap kekeringan karena panjang akar tidak berkorelasi secara signifikan dengan bobot buah per tanaman pada 50% dan 25% kapasitas lapang. Berdasarkan korelasi dan analisis lintas pada kondisi normal (100% kapasitas lapang) dan kondisi tercekam (50% kapasitas lapang), karakter jumlah buah dan persentase fruit set berkorelasi positif dan berpengaruh langsung terhadap bobot buah per tanaman sehingga dapat digunakan sebagai kriteria seleksi untuk perbaikan tanaman cabai yang toleran kekeringan.KeywordsCekaman air; Cekaman kekeringan; Kriteria seleksi; Cabai; Capsicum annuum LAbstractWater stress is one of limiting factors for plant growth and production, including chili (Capsicum annuum L.). Plant productivity is a complex character so the relationship between characters needs to be known to get the right selection criteria for crop improvement. This study aimed to determine the selection criteria for the production of drought-tolerant chili pepper at the flowering phase. The research arranged under completely randomized design with three levels of water stress, namely 100% water field capacity (control), 50% water field capacity (medium stress) and 25% water field capacity (extreme stress). The results of the correlation analysis showed that the various characters observed exhibited differences associations with drought stress levels. Root length cannot be used for selection criterium of drought tolerant on chili because it was no positive significantly correlated to fruit weight per plant at 50% and 25% water field capacity. Based on correlation and path analysis in normal and drought conditions, the number of fruits and percentage of fruit set can be used as selection criteria for genetic improvement of drought-tolerant on chili plants because these characters were significantly positively correlated and had a direct effect on fruit weight of the plant.

Korelasi Sifat-Sifat Agronomi dengan Hasil dan Kandungan Antosianin Padi Beras Merah Hafidh Prabowo; Djati Waluyo Djoar; Parjanto Parjanto
Agrosains : Jurnal Penelitian Agronomi Vol 16, No 2 (2014): Agrosains: Jurnal Penelitian Agronomi
Publisher : Universitas Sebelas Maret

Brown rice is a type of rice can be grown on land that is less water (upland). Brown rice has anthocyanin as an antioxidant. The purpose of this study was to identify brown rice agronomics characters that can be used as selection criteria to obtain brown rice which high anthocyanin. The experiment was conducted at the greenhouse of Agriculture Faculty, UNS using completed randomized block design consisting of 5 local varieties namely Mandel Wonogiri, Segreng Gunung Kidul, Segreng Boyolali, Segreng Wonogiri 1, and Segreng Wonogiri 2. Analysis of the data using the model Singh and Chaudhary (1979) are displayed in the form of a matrix correlation. Anthocyanin assays using the differential pH test method. The results showed that there was no difference between the anthocyanin content of varieties, but there is a tendency Segreng Boyolali varieties containing anthocyanins and Mandel Wonogiri higher. In general, varieties tested showed different potential yield, except Mandel Wonogiri varieties have low yield. The character of the total number of tillers, panicle length, and number of panicles per hill showed a strong correlation coefficient with the results. Plant height, number of panicles per hill, and the weight of 1000 seeds showed a high correlation coefficient with anthocyanin content.

Identifikasi Morfologi Aksesi Srikaya (Annona squamosa) di Gedangsari Gunungkidul Danang Setiono; Parjanto Parjanto; Djati Waluyo Djoar
Agrosains : Jurnal Penelitian Agronomi Vol 15, No 2 (2013): Agrosains: Jurnal Penelitian Agronomi
Publisher : Universitas Sebelas Maret

The purpose of this study to determine the morphological characteristics and variability of planting sugar apple Gedangsari, Gunungkidul, Yogyakarta. Identify the morphological characteristics conducted on 36 plant samples (36 accessions) based method on stratified sampling and purposive sampling. The data were analyzed descriptively and clustering analysis. The results of the research showed that sugar-apple had pyramida canopy shape, plant height of 264±525 cm, oblongus leaves type, integer leaves margins, acuminatus leaves tip form, base acutus leaves, petiole cylindrical shape, dark green petiole, dark green color of the upper surface leaves, faded green color of the lower surface, leaves length 16.06±8.23 cm, leaves width 3,73±6,9 cm, leaves area 24,28±85,71 cm2, round shaped of rod, patens branching pattern, stem circumference 17,5±38 cm, axillary flos of flower layout, having 3 petals, whitish green of flower color, is cordate and round shape of fruit, fruit weight of 83,03±180,3 g, fruit circumference 22,4±19,36 cm, flesh thick 1,73±3,66 mm, whitish green and green of fruit color, the total amount of fruit scales 70±104,66, and the value of PTT between 18-29 obrix, blunt shape of fruit scales, ellipsoid shape of seeds, number seeds 7±70,66 and total seed weight 2,3±19,86g. There were variability include leaves length, leaves width, leaves area, stem girth, fruit weight, fruit girth, fruit flesh thickness, number of fruit scales, PTT values and fruit shape and the fruit skin’s color. The result of clustering analysis showed that similarity coefficient between the accessions was 66%.

PEMBENTUKAN VARIETAS SIRSAK UNGGUL MELALUI PEMULIAAN POLIPLOIDI: INDUKSI POLIPLOIDI TANAMAN SIRSAK DENGAN KOLKISIN Parjanto Parjanto
Caraka Tani: Journal of Sustainable Agriculture Vol 27, No 2 (2012): October
Publisher : Universitas Sebelas Maret

Penelitian ini bertujuan membentuk sirsak unggul melalui serangkaian penelitian pemuliaan poliploidi secara bertahap; tahap pertama bertujuan mempelajari pengaruh perlakukan kolkisin terhadap fenotipe dan jumlah kromosom bibit sirsak sekaligus membentuk tanaman sirsak poliploid. Penelitian dilakukan melalui percobaan berulangan untuk menguji pengaruh perlakukan kolkisin (0,10%, 0,15%, dan kontrol/tanpa kolkisin) terhadap fenotipe bibit dan jumlah kromosom sirsak. Variabel-variabel yang diamati adalah tinggi tanaman, jumlah daun, dan kromosom. Hasil penelitian menunjukkan bahwa: (1) perlakukan kolkisin 0,1 % dan 0,15 % tidak berpengaruh terhadap tinggi tanaman dan jumlah daun pada pertumbuhan awal (vegetatif), belum terindentifikasi tanaman poliploid berdasar pengamatan fenotipe; (2) tanaman srikaya diploid mempunyai susunan kromosom (rumus kariotipe) 2n = 14 = 12 m + 2 sm atau n = 7 = 6 m + 1 m (terdiri atas 6 pasang kromosom metasentris, 1 pasang kromosom submetasentris).

Keanekaragaman Pertumbuhan dan Hasil Tanaman Oyong (Luffa Acutangula L.) pada Berbagai Konsentrasi Kolkhisin Adib Fauzan Rahman; Nandariyah Nandariyah; Parjanto Parjanto
Agrotechnology Research Journal Vol 1, No 1 (2017): Agrotechnology Research Journal
Publisher : Perkumpulan Agroteknologi/Agroekoteknologi Indonesia

Oyong production is still low due to the limited availability of quality seeds. The production can be increased by one of the plant breeding techniques, which is mutation technique. The technique of mutation by chemical mutagens is able to increase the genetic diversity ofplants allowing the breeding to select genotype of plants in accordance with the intended breeding objective. Therefore, research is required to be conducted by using colchicin as a chemical mutagen which causes polyploid, in which organism has three sets or more chromosomes inside its cells. Meanwhile, the general nature of this polyploid plant is being sturdier; parts of the plantare bigger; so that later, its poor nature will be better as well as changing the potency of the result. Research using complete randomized block design (RBD) with one factor concentration of 0,1% (P1), 0,2% (P2), 0,3% (P3), 0,4% (P4), 0,5% ( P5), and 0,6% (P6). Colchicines treatment can cause changes in growth and yield variables. Colchicines treatment with a concentration of 0,3% showed positive changes to theweight of fruit, fruit diameter and length of the fruit. An increase in diversity on growth and yield components especially on colchicines treatment with 0,3%concentration.

Karakterisasi Morfologi Tetua dan Hybrid Anggrek Dendrobium bigibbum dan Dendrobium lineale Sri Hartati; Nandariyah Nandariyah; Endang Setia Muliawati; Sukaya Sukaya; Endang Yuniastuti; Parjanto Parjanto; Ida Rumia Manurung
Agrosains : Jurnal Penelitian Agronomi Vol 24, No 2 (2022): Agrosains: Jurnal Penelitian Agronomi
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/agsjpa.v24i2.65255

Dendrobium are one of the most cultivated ornamental orchids plants. However, orchids as genetic resources have not been optimally utilized as parents in crossing to produce offspring according to the characteristics desired by consumers. This study aims to study the similarities between the Hybrid results of crosses compared to their parent orchids and obtain a new variety of Hybrid from crosses of Dendrobium bigibbum and Dendrobium lineale. This research was conducted at the Bogor Botanical Gardens and Plosorejo Village, Matesih District, Karanganyar Regency, Central Java. The materials used were 14 samples of orchid plants, including three examples of D. bigibbum orchids, three samples of D. lineale orchids, four samples of Hybrid plants resulting from crosses of ♀ D. bigibbum X ♂ D. lineale, and four samples of Hybrid plants from crossing ♀ D. lineale X ♂ D. bigibbum. Observation variables include quantitative variables. The quantitative morphological variables use the guideline developed by BALITHI. To analyze the data using the program of NTSYSpc 2.02i version. The results showed that based on quantitative observations, Dendrobium lineale as a female parent can change the size of the dorsal sepal width, flower arrangement length, lateral sepal length, and flower length. And Dendrobium bigibbum as the female parent can change the size of the petal length, lateral sepal width, flower width, pseudobulb thickness, pseudobulb length, leaf length, and leaf width.

Yield of the Mutant (M6) Short Stem of Mentik Wangi Rice Varieties Resulting from Gamma Ray Irradiation 300 Gray Ahmad Yunus; Rizky Mulyawati; Parjanto Parjanto
Journal of Biodiversity and Biotechnology Vol 1, No 2 (2021)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v1i2.56437

Mentik Wangi is one of Indonesia's most popular rice germplasm sources due to its delicate, fluffy texture and arom aroma. Farmers switch to better varieties with high yields due to field challenges in cultivating fragrant rice. The plants are quite tall, harvest time is long, and yield is low; therefore farmers switch to superior varieties with high yields. The purpose of this research was to obtain information on how much yield of 14 mutant rice lines produce and to find mutant lines with short stems and high yields that could be developed to new varieties. The research was conducted at the Tegalgondo Rice Seed Garden Agricultural Land between June to October 2020. This study used a Completely Randomized Block Design (CRBD) with a single factor. Each treatment was repeated three times. The data obtained analyzed with analysis of variance and followed with Duncan Multiple Range Test at 5% level if any significant influences were obtained. The results of this research showed that 14 Mentik Wangi rice lines yields were ranging from 6.70 to 8.21 tons ha-1.Stems from 9 lines with high yields were M6-MW3-G10-14-2, M6-MW3- G12-1-17, M6-MW3-G5-21-3, M6-MW3-G5-21-14, M6-MW3-G6-17-14-4, M5-MW3-G6-20-9-16, M6-MW3-G1-1-2 M5-MW3-G1-5-47, M6-MW3-G6-10-9-26. These lines were potential candidates for new high-yielding varieties, which would then considered to be evaluated for higher yields or multilocation tests before becoming an official new varieties.Keywords: mentik wangi; mutations; short stem; yield potential;

Genetic of Salak Pondoh, Gading Varieties and Its Hybrids Based on RAPD Markers Nandariyah Nandariyah; Parjanto Parjanto; Pinaka Pinasti Ratu
Journal of Biodiversity and Biotechnology Vol 1, No 1 (2021)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v1i1.50396

A molecular marker of parent and offspring is used to find fast and accurate markers influenced by DNA isolation and amplification. This research aims to find the most suitable DNA isolation and DNA amplification methods. This study used four DNA isolation methods; namely IM01, IM02, IM03, and IM04. DNA amplification used ten protocols (AP01, AP02, AP03, AP04, AP05, AP06, AP07, AP08, AP09, and AP010). The results of the research showed that the most suitable DNA isolation method for salak was IM0, and the most suitable DNA amplification for salak was AP04 that produces the highest value of DNA bands. Keywords: DNA isolation; DNA amplification; hybrids.

DIVERSITY OF SAPODILLA (ACHRAS ZAPOTA L.) GROWTH FROM WONOGIRI AND BOJONEGORO IN DIFFERENT AGE OF TRANSPLANTATION SEED Endang Yuniastuti; Muhammad Agung; Parjanto Parjanto
Journal of Biodiversity and Biotechnology Vol 2, No 1 (2022)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v2i1.61170

Most farmers determine the cutting of air layer seed in a corp through the amount of the growing roots from air layer itself. The roots can be an indicator of air layer cutting method in a corp. The roots, which are too old or too young, grow less optimal in the air layer seed after being transferred into another cultivated medium. It is necessary to have knowledge about the precise age of air layer cutting so that the vegetative propagation by using air layer can be performed accurately and efficiently. The treatment that is executed is the age of air layer and the corps accession. The result of this research indicates that the effect of the difference air layer age on plant growth is at the age of emerging buds, aged 180 Day is treated with the age of the fastest emerging shoots. The growth of Bojonegoro air layer plants results that does not differ with Wonogiri air layer plants. There is no interaction between air layer age with sapodilla accession on plant growth.

Keragaman Genetik Sirih Cina (Peperomia pellucida L.) Berdasarkan Penanda RAPD (Random Amplified Polymorphic DNA) Nandariyah Nandariyah; Hardian Ningsih; Annisa Nur Fadhillah; Parjanto Parjanto; Edi Paryanto
Agrosains : Jurnal Penelitian Agronomi Vol 26, No 1 (2024): Agrosains: Jurnal Penelitian Agronomi
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/agsjpa.v26i1.92694

Chinese betel is a plant widely found in Indonesia, especially in the ex-Karasidenan area of Surakarta. The existence of growing Chinese betel can be found at various altitudes with humid growing conditions with low sun intensity. People in Surakarta believe that Chinese betel can be used as a traditional medicine. Chinese betel nut is suspected to contain a minerals and bioactive substances that are beneficial to health. Currently, there are few references to Chinese betel, especially related to genetic diversity. This study aims to study the diversity of Chinese betel band patterns based on RAPD markers using OPA-3, OPA-4, OPA-11, OPC-05, and OPD-4 primers. This research was carried out at the Plant Breeding Laboratory, Faculty of Agriculture, Gadjah Mada University, Yogyakarta in July - November 2023 using Chinese betel leaf DNA taken from various places in the ex-karasidenan area of Surakarta. The results of the data analyzed by NTSYSpc version 2.02 with the SimQual (Similarity for Qualitative Data) function using the UPGMA (Unweighted Pair Group with Arithmetic Averaging) method based on the Jaccard similarity index value. The amplification results showed 30 bands with 13 polymorphic bands and 17 monomorphic bands. The level of polymorphism among Chinese betel samples in the five RAPD primers used presented 44.36% of the total 13 polymorphic bands. Jaccard's genetic similarity matrix data ranged from 0.667 – 0.964. The results of the dendogram show that the sample is divided into two clusters with a diversity index of 0.28.

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