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Rancang Bangun Mesin Pencacah Limbah Kotoran Sapi Arifin, Zainal; Prasetyo, Singgih Dwi; Triyono, Teguh; Harsito, Catur; Yuniastuti, Endang
Jurnal Rekayasa Mesin Vol 11, No 2 (2020)
Publisher : Jurusan Teknik Mesin, Fakultas Teknik, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jrm.2020.011.02.6

Cattle farms produce waste which consisted of cow excrement in the form of solid, liquid, gas or cattle feed residual. Utilization of cow manure waste into organic compost requires proper processing technology which includes the technology of solid manure pulverizing and the technology of mixing raw materials. This research focuses on designing a cattle dung waste pulverizer. The methods used are numerical calculations, simulations using the Fusion 360 software, and fabrication based on Design for Manufacture and Assembly (DFMA). The main parts of the design include pulverizer, engine frame, cover (input and output hoppers), diesel motor, transmission belt and pulley. The safety factor obtained for the frame section was 4,645, for the cover section was 11.3, and for the counter section was 2,009 with assumptions of uniform load distribution of 20000 N. The power required for the design was 6.6 HP. The cattle dung waste pulverizer was built and tested using a diesel motor with a maximum power of 13 HP. The cattle dung waste pulverizer's capacity was 1500 kg/hour.

Keragaman Kacang Gude Putih (Cajanus cajan [L.] Millsp.) di Kabupaten Wonogiri, Jawa Tengah Endang Yuniastuti; Sukaya Sukaya; Rhizia Syifa Fauziyah; Marshelina Noor Indah Delfianti
Buletin Plasma Nutfah Vol 26, No 1 (2020): JUNE
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v26n1.2020.p51-62

Kacang gude (Cajanus cajan) termasuk dalam tanaman kacang-kacangan yang memiliki potensi untuk digunakan sebagai alternatif untuk ketahanan pangan karena kaya akan protein, karbohidrat, dan memiliki toleransi tinggi terhadap stres lingkungan. Informasi tentang kacang gude di Indonesia masih minim sehingga perlu dilakukan eksplorasi dan karakterisasi. Tujuan penelitian ini adalah mempelajari keragaman karakter kacang Gude Putih (Cajanus cajan [L.] Millsp.) di Wonogiri, Jawa Tengah. Penelitian ini dilakukan di Desa Sidokarto, Doho, dan Gambiran, Kecamatan Girimarto, Kabupaten Wonogiri, Provinsi Jawa Tengah. Variabel yang diamati meliputi morfologi batang, daun, bunga, polong, dan biji. Data morfologis dianalisis menggunakan uji chi kuadrat dan program NTSYS. Hasil penelitian menunjukkan bahwa karakteristik morfologi kacang Gude Putih di tiga lokasi di Wonogiri memiliki variasi genetik. Karakter tinggi tanaman, jumlah cabang, dan ketebalan batang di ketiga lokasi memiliki hasil yang beragam sedangkan karakter lainnya memiliki hasil yang seragam. Hasil analisis hubungan antara lokasi dan karakter kacang Gude Putih bersifat signifikan pada beberapa karakter termasuk tinggi tanaman, jumlah cabang, dan ketebalan batang. Koefisien kesamaan tanaman kacang Gude Putih di tiga lokasi di Wonogiri berkisar antara 83–87% hingga 100%. Nilai koefisien keragaman pada kisaran 13–17%, menunjukkan bahwa keragaman kacang Gude Putih di tiga lokasi tersebut memiliki keanekaragaman yang sempit.

Analisis genotip normal dan abnormal pada klon kelapa sawit (Elaeis guineensis Jacq.) dengan Amplified Fragment Length Polymorphism (AFLP) Analysis normal and abnormal genotypes of oil palm clones (Elaeis guineensis Jacq.) by Amplified Fragment Length Polymorphism (AFLP) Nurita TORUAN-MATHIUS; . ENDANG-YUNIASTUTI; Ridwan SETIAMIHARJA; Murdaningsih H. KARMANA
E-Journal Menara Perkebunan Vol 73, No 1: Juni 2005
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

SummaryTissue culture-derived plants of oil palmmay develop abnormal flowers in whichprimordial stamens are converted into carpel-liketissue or mantled fruits, and sterile male flowers.This abnormality can be heritable, individualpalm may show variation in mantling andreversion to the normal phenotype over time hasbeen observed. The aim of these experiments wasto analyze the differences between normal andabnormal genotypes by DNA-AFLP. DNA wasisolated from young fruits of three clones,MK152, MK209, and MK 212 each of themconsisted of normal fruits, abnormal fruits andsterile male flowers. The research consisted of (i)selection of AFLP primer which can producepolymorphic bands, (ii) genetic similaritiesanalysis, UPGMA, principal component analysisand specific DNA bands between normal orabnormal genotypes. For primers selection, 20AFLP primers with DNA from MK 152 normaland abnormal genotypes were used. The selectedprimers were then used to amplify DNA of ninegenotypes. The results show that 10 primer com-binations EcoRI/MseI produced polymorphicbands. Each primer from 10 primer producedonly one or two DNA bands indicates that thedifferences between normal and abnormalgenotypes in the same clone. However, nopolymorphism was consistently found betweennormal and abnormal clones in all the sets.Genetic similarity analysis shows that betweengenotype had high genetic similarities, around92-99%. The results of UPGMA found thedifferent clustering between normal fruit,abnormal male and abnormal fruits. The resultsshow same as clustering based on first, secondand third component. This suggest that, whilstAFLP method is an effective way of detectingvariation in tissue culture-derived plants,different approaches are required to identify thecasual basis of the mantled fruit abnormality.RingkasanTanaman kelapa sawit yang dihasilkan darikultur jaringan, umumnya dalam perkembangan-nya akan memiliki organ reproduktif yangabnormal. Abnormalitas berupa primordialstamen berkembang menjadi bentuk jaringanseperti karpel, buah mantel, atau bunga jantanmandul. Penelitian ini bertujuan untukmendapatkan pembeda DNA-AFLP antaragenotip normal dan abnormal pada klon-klonkelapa sawit. DNA diisolasi dari buah muda klonMK 152, MK 209, dan MK 212 yang masing-masing terdiri atas genotip normal, berbuahabnormal, dan berbunga jantan steril. Percobaanmencakup (i) seleksi primer AFLP yang mampumenghasilkan pita yang polimorfis, (ii) analisiskemiripan genetik, UPGMA, komponen utamadan pita pembeda antar genotip normal danabnormal. Seleksi primer dilakukan terhadap 20primer AFLP menggunakan DNA dari genotipMK 152 yang normal dan abnormal. Selanjutnyaprimer terpilih digunakan untuk mengamplifikasiDNA dari kesembilan genotip yang diuji. Hasilyang diperoleh menunjukkan bahwa 10 kombi-nasi primer EcoRI/MseI mampu menghasilkanpita yang polimorfis. Dari 10 primer yang diuji,masing-masing hanya menghasilkan satu ataudua pita DNA yang mampu membedakan genotipnormal dan abnormal dalam klon yang sama.Namun, tidak ada pita DNA spesifik yangmampu membedakan genotip normal denganabnormal untuk seluruh klon yang diuji. Analisiskemiripan genetik menunjukkan bahwa antargenotip memiliki kemiripan genetik yang sangattinggi, yaitu 92-99%. Dari hasil UPGMAdiperoleh pengelompokan yang terpisah antargenotip normal, abnormal jantan dan buahabnormal. Hasil tersebut didukung olehpengelompokan berdasarkan komponen utamasatu, dua dan tiga. Dapat disimpulkan bahwa,teknik AFLP tidak efektif untuk mendeteksipembeda antar genotip tanaman yang diperolehdari kultur jaringan, pendekatan lainnyadiperlukan untuk mengidentifikasi abnormalitas.

Persilangan Backcross 2 (BC 2) Galur Harapan Padi Hitam/Jeliteng//Jeliteng Arifah - Husna; Nandariyah - Nandariyah; Endang - Yuniastuti; Amalia Tetrani Sakya
Vegetalika Vol 11, No 3 (2022)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/veg.73833

Beras hitam saat ini mulai populer sebagai pangan fungsional seiring dengan kesadaran akan pentingnya kesehatan. Namun, beberapa kendala budidaya mempengaruhi minat petani dalam memproduksi beras hitam, seperti umur tanaman yang panjang, kerentanan terhadap WBC, dan produksi yang rendah. Persilangan dapat dilakukan untuk membentuk tanaman baru dengan sifat yang diinginkan. Metode silang balik digunakan untuk memperbaiki varietas yang sudah mempunyai karakter yang baik, tetapi kurang unggul pada beberapa karakter. Penelitian ini dilakukan untuk memperoleh hasil persilangan backcross 1 dengan tetua (Padi Hitam Jeliteng) sehingga dapat dihasilkan benih generasi backcross 2 untuk dilanjutkan pada backcross generasi berikutnya. Metode persilangan yang digunakan adalah persilangan balik antara 6 nomor (BC1GH8; BC1GH13; BC1GH44; BC1GH46; BC1GH51; BC1GH52) hasil persilangan BC1 antara galur-galur potensial galur padi hitam/Jeliteng//Jeliteng yang disilangkan lagi dengan salah satu tetua, yaitu varietas Jeliteng. Seluruh data hasil pengamatan dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa terdapat perbedaan hasil pada variabel umur berbunga, masa pengisian bulir gabah, dan persentase keberhasilan persilangan. Persilangan backcross 2 menghasilkan keturunan F1 Backcross 2 yang bersifat fertil dan dapat digunakan sebagai tetua untuk persilangan backcross generasi berikutnya. Rata-rata persentase keberhasilan dari keseluruhan hasil set persilangan backcross 2 adalah 44,18%. Penelitian ini diharapkan memperoleh hasil persilangan backcross 1 dengan tetua (Padi Hitam Jeliteng) sehingga dapat dihasilkan benih generasi backcross 2 untuk dilanjutkan pada backcross generasi berikutnya.

Hubungan Kekerabatan Genetik Rambutan (Nephelium lappaceum L.) Berdasarkan Lima Marka RAPD (Random Amplified Polimorphic DNA) Fitriyanti, Rafika Indah; Yuniastuti, Endang; Nandariyah, Nandariyah
Agrikultura Vol 34, No 2 (2023): Agustus, 2023
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/agrikultura.v34i2.41258

Rambutan (Nephelium lappaceum L.) merupakan salah satu tanaman tropis Indonesia yang mempunyai banyak potensi ekonomi sebagai upaya diversitas buah-buahan. Pelestarian plasma nutfah rambutan perlu dilakukan lebih lanjut baik secara kualitas maupun kuantitas. Metode RAPD digunakan untuk memperoleh informasi mengenai keragaman genetik antar jenis tanaman rambutan. Penelitian dilakukan menggunakan metode RAPD (Random Amplified Polymorphic DNA) dengan 20 primer. Hasil penelitian menunjukkan bahwa seleksi 20 primer menghasilkan 5 primer terbaik yaitu (OPD 2, OPD 5, OPD 6, OPD 10, OPD 12), pada ukuran 50-2000 bp dengan total 48 pita (42 pita polimorfik dan 6 pita monomorfik). Tingkat polimorfisme keragaman antar jenis rambutan sebesar 86,24%. Hasil uji dendogram UPGMA menunjukkan adanya keragaman genetik yang tergolong tinggi pada rambutan. Terbentuk dua klaster utama dengan tingkat kesamaan antara 56-77%. Rambutan jenis Rapiah berkerabat dekat dengan Sibatuk Ganal sebesar 77%.

Analisis genotip normal dan abnormal pada klon kelapa sawit (Elaeis guineensis Jacq.) dengan Amplified Fragment Length Polymorphism (AFLP) Analysis normal and abnormal genotypes of oil palm clones (Elaeis guineensis Jacq.) by Amplified Fragment Length Polymorphism (AFLP) Nurita TORUAN-MATHIUS; . ENDANG-YUNIASTUTI; Ridwan SETIAMIHARJA; Murdaningsih H. KARMANA
Menara Perkebunan Vol. 73 No. 1: 73 (1), 2005
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v73i1.159

SummaryTissue culture-derived plants of oil palmmay develop abnormal flowers in whichprimordial stamens are converted into carpel-liketissue or mantled fruits, and sterile male flowers.This abnormality can be heritable, individualpalm may show variation in mantling andreversion to the normal phenotype over time hasbeen observed. The aim of these experiments wasto analyze the differences between normal andabnormal genotypes by DNA-AFLP. DNA wasisolated from young fruits of three clones,MK152, MK209, and MK 212 each of themconsisted of normal fruits, abnormal fruits andsterile male flowers. The research consisted of (i)selection of AFLP primer which can producepolymorphic bands, (ii) genetic similaritiesanalysis, UPGMA, principal component analysisand specific DNA bands between normal orabnormal genotypes. For primers selection, 20AFLP primers with DNA from MK 152 normaland abnormal genotypes were used. The selectedprimers were then used to amplify DNA of ninegenotypes. The results show that 10 primer com-binations EcoRI/MseI produced polymorphicbands. Each primer from 10 primer producedonly one or two DNA bands indicates that thedifferences between normal and abnormalgenotypes in the same clone. However, nopolymorphism was consistently found betweennormal and abnormal clones in all the sets.Genetic similarity analysis shows that betweengenotype had high genetic similarities, around92-99%. The results of UPGMA found thedifferent clustering between normal fruit,abnormal male and abnormal fruits. The resultsshow same as clustering based on first, secondand third component. This suggest that, whilstAFLP method is an effective way of detectingvariation in tissue culture-derived plants,different approaches are required to identify thecasual basis of the mantled fruit abnormality.RingkasanTanaman kelapa sawit yang dihasilkan darikultur jaringan, umumnya dalam perkembangan-nya akan memiliki organ reproduktif yangabnormal. Abnormalitas berupa primordialstamen berkembang menjadi bentuk jaringanseperti karpel, buah mantel, atau bunga jantanmandul. Penelitian ini bertujuan untukmendapatkan pembeda DNA-AFLP antaragenotip normal dan abnormal pada klon-klonkelapa sawit. DNA diisolasi dari buah muda klonMK 152, MK 209, dan MK 212 yang masing-masing terdiri atas genotip normal, berbuahabnormal, dan berbunga jantan steril. Percobaanmencakup (i) seleksi primer AFLP yang mampumenghasilkan pita yang polimorfis, (ii) analisiskemiripan genetik, UPGMA, komponen utamadan pita pembeda antar genotip normal danabnormal. Seleksi primer dilakukan terhadap 20primer AFLP menggunakan DNA dari genotipMK 152 yang normal dan abnormal. Selanjutnyaprimer terpilih digunakan untuk mengamplifikasiDNA dari kesembilan genotip yang diuji. Hasilyang diperoleh menunjukkan bahwa 10 kombi-nasi primer EcoRI/MseI mampu menghasilkanpita yang polimorfis. Dari 10 primer yang diuji,masing-masing hanya menghasilkan satu ataudua pita DNA yang mampu membedakan genotipnormal dan abnormal dalam klon yang sama.Namun, tidak ada pita DNA spesifik yangmampu membedakan genotip normal denganabnormal untuk seluruh klon yang diuji. Analisiskemiripan genetik menunjukkan bahwa antargenotip memiliki kemiripan genetik yang sangattinggi, yaitu 92-99%. Dari hasil UPGMAdiperoleh pengelompokan yang terpisah antargenotip normal, abnormal jantan dan buahabnormal. Hasil tersebut didukung olehpengelompokan berdasarkan komponen utamasatu, dua dan tiga. Dapat disimpulkan bahwa,teknik AFLP tidak efektif untuk mendeteksipembeda antar genotip tanaman yang diperolehdari kultur jaringan, pendekatan lainnyadiperlukan untuk mengidentifikasi abnormalitas.

The Influence of Altitude Toward Vegetative and Generative Growth of Sugar Palm (Arenga pinnata) Akbar, Difa Adiba; Yuniastuti, Endang; Supriyono, Supriyono
Agro Bali : Agricultural Journal Vol 8, No 1 (2025)
Publisher : Universitas Panji Sakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37637/ab.v8i1.1981

Sugar palm is a member of the palm family that offers many benefits. Palm sugar is produced from the male flower, while the fruit is produced from the female flower. The potential of this resource has not been fully realized because most of it is sourced from the wild. This research aims to determine the differences in the growth of palms based on altitude. The research was conducted from October 2023 to March 2024 in Sleman, Kulon Progo, and Purworejo regions. Treatment of altitude is divided into three, namely lowlands (0-400 masl), medium (401-700 masl), and highlands (>700 masl). The parameters used in this research were plant height, stem, number of leaves, leaf width and length, number of productive and unproductive leaves, number of bunches, number of female and male flowers, and number and weight of fruits. Data on vegetative growth were analyzed using ANOVA, and then LSD for further and data on generative growth used the Kruskal Wallis test with a significance level of 95%. The result showed a significant difference between vegetative growth and altitude except for the number of productive leaves and generative growth; there was only a significant difference in the number of male flowers.

Developing Interactive Digital Modules on Discovery Learning to Improve Students Learning Interest: Developing Discovery Learning-based Interactive Digital Modules to Increase Students’ Learning Interest Hanatan, Rianhe Binthariningrum; Yuniastuti, Endang; Prayitno, Baskoro Adi
Jurnal TEKNODIK Jurnal Teknodik Vol. 27 No. 1, Juni 2023
Publisher : Pusat Data dan Teknologi Informasi Kementerian Pendidikan Kebudayaan, Riset dan Teknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32550/teknodik.vi.862

Pembelajaran sistem daring pada masa pandemi Covid-19 menyebabkan menurunnya minat belajar siswa yang dipantau dari kegiatan observasi pada kelas eksperimen. Penurunan minat belajar siswa didukung oleh data studi literatur dan hasil data observasi pada kelas eksperimen. Penambahan suplemen Modul Digital Interakif sebagai media pembelajaran merupakan sarana pelengkap untuk menambah maksimalnya proses daring dengan model Discovery Learning. Penelitian ini bertujuan untuk: 1) mengetahui kelayakan Modul Digital Interaktif berbasis model Discovery Learning; dan 2) meningkatkan minat belajar siswa. Penelitian ini merupakan penelitian pengembangan dengan model 4D yang diringkas dan dioptimalkan menjadi 3D. Sampel penelitian dipilih secara purposive sampling yaitu pada kelas XI MIPA 2 berjumlah 35 siswa SMA Negeri I Karanganyar tahun pelajaran 2020/2021. Instrumen penelitian meliputi perangkat pembelajaran, lembar observasi, panduan wawancara, dan angket. Angket terdiri dari validasi penilaian ahli angket, validasi penilaian ahli materi, validasi penilaian ahli media, validasi penilaian ahli pembelajaran, validasi penilaian ahli praktisi, angket minat belajar, angket analisis kebutuhan siswa dan angket respons siswa terhadap media. Teknik analisis data terdiri dari deskriptif kualitatif dan deskriptif kuantitatif menggunakan analisis Rasch di aplikasi Winstep 3.73. Hasil dari penelitian adalah: 1) Modul Digital Interaktif berkategori sangat layak digunakan dalam pembelajaran berdasarkan hasil penilaian ahli praktisi, ahli pembelajaran, ahli media, dan ahli materi; dan 2) ada peningkatan minat belajar siswa berdasarkan hasil analisis data pre-angket dan post-angket minat belajar yang dianalisis menggunakan teknik analisis Rasch dengan metode stacking. Online learning system during the covid-19 pandemic era has impacted students’ learning interest to decrease as of the observation on experiment class. The decrease of students’ learning interest is confirmed by the data of literary review and the observation result on the experiment class. Adding the supplement of Interactive Digital Module as learning media is a complement to maximize the online learning process with Discovery Learning model. The objectives of this research are 1) to know the feasibility of Discovery Learning-based Interactive Digital Modules; and 2) to increase students’ learning interest. This research is a development research with 4D model which is summed up into 3D. The sample of this research is done with purposive sampling method, i.e. 35 students of MIPA XI Class in SMA Negeri 1 Karanganyar the academic year of 2020/2021. The instruments applied in this research are learning equipment, observation form, interview guidance, and questionnaire. The questionnaires consist of validation by questionnaire experts, validation by material experts, validation by media experts, validation by educational experts, validation by practitioners, learning interest questionnaire, students’ need questionnaire, and students’ to-media-response questionnaire. Data analysis technique are qualitative descriptive and quantitative descriptive by using Rasch analysis technique in the application of Winstep 3.73. The result of this research are that: 1) Interactive Digital Modules are very feasible to be used in learning process based on the evaluation by practitioners, educational experts, media experts, and material experts; and 2) there is an increase on students’ learning interest based on the analysis results on the data of pre-questionnaire and post-questionnaire by Rasch analysis technique with stacking method.

Colchicine-Mediated Polyploidization For Improvement Of French Marigold Traits Aziz Nur Arifin; Parjanto; Endang Yuniastuti
International Journal of Interdisciplinary Research Vol. 2 No. 2 (2026): Vol 2 no 2 July 2026
Publisher : Ponpes As-Salafiyyah Asy-Syafi'iyyah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.71305/ijir.v2i2.1629

This study evaluated the effects of colchicine concentration and soaking duration on vegetative morphology of French marigold (Tagetes patula L.) cv. Janie Spry, a naturally allotetraploid species (2n = 4x = 48). A 4×3 factorial completely randomized design was employed with four colchicine concentrations (0%, 0.1%, 0.2%, 0.3%) and three soaking durations (4, 8, 12 hours), totaling 12 treatments with three replications (n = 36). Germinated seeds at the radicle stage were soaked in colchicine solutions and grown in a greenhouse. Survival rate, plant height, stem diameter, and number of shoots were recorded during the vegetative phase. Two-way ANOVA revealed highly significant interaction effects (p<0.001) of concentration and duration on survival rate and shoot number, while plant height and stem diameter were not significantly affected. Survival declined sharply at higher concentrations with prolonged soaking, reaching only 30% at 0.3% colchicine for 12 hours. The 0.2% colchicine at 4-hour soaking treatment was identified as optimal, maintaining 90% survival while increasing shoot number by 37.7% relative to the control (30.7 vs. 22.3 shoots plant⁻¹). The highest shoot count (34.3 shoots plant⁻¹) occurred at 0.3% for 12 hours but was accompanied by unacceptable seedling mortality. These results indicate that moderate colchicine concentrations with short exposure offer an effective balance between morphological modification and seedling viability in T. patula. Cytological confirmation and floral trait evaluation will be reported in a subsequent publication.

Karakterisasi Morfologi Tetua dan Hybrid Anggrek Dendrobium bigibbum dan Dendrobium lineale Hartati, Sri; Nandariyah, Nandariyah; Muliawati, Endang Setia; Sukaya, Sukaya; Yuniastuti, Endang; Parjanto, Parjanto; Manurung, Ida Rumia
Agrosains : Jurnal Penelitian Agronomi Vol 24, No 2 (2022): Agrosains: Jurnal Penelitian Agronomi
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/agsjpa.v24i2.65255

Dendrobium are one of the most cultivated ornamental orchids plants. However, orchids as genetic resources have not been optimally utilized as parents in crossing to produce offspring according to the characteristics desired by consumers. This study aims to study the similarities between the Hybrid results of crosses compared to their parent orchids and obtain a new variety of Hybrid from crosses of Dendrobium bigibbum and Dendrobium lineale. This research was conducted at the Bogor Botanical Gardens and Plosorejo Village, Matesih District, Karanganyar Regency, Central Java. The materials used were 14 samples of orchid plants, including three examples of D. bigibbum orchids, three samples of D. lineale orchids, four samples of Hybrid plants resulting from crosses of ♀ D. bigibbum X ♂ D. lineale, and four samples of Hybrid plants from crossing ♀ D. lineale X ♂ D. bigibbum. Observation variables include quantitative variables. The quantitative morphological variables use the guideline developed by BALITHI. To analyze the data using the program of NTSYSpc 2.02i version. The results showed that based on quantitative observations, Dendrobium lineale as a female parent can change the size of the dorsal sepal width, flower arrangement length, lateral sepal length, and flower length. And Dendrobium bigibbum as the female parent can change the size of the petal length, lateral sepal width, flower width, pseudobulb thickness, pseudobulb length, leaf length, and leaf width.

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