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All Journal Jurnal Ilmu Ternak Veteriner Hermeneutik : Jurnal Ilmu Al-Qur'an dan Tafsir Mestro
Yudi Setiadi
Pusat Teknologi Nuklir Bahan dan Radiometri - BATAN
Religion Humanities Automotive Engineering Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Electrical & Electronics Engineering Engineering Industrial & Manufacturing Engineering Veterinary

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Development of fowl cholera vaccine: I. Protection of Pasteurella multocida local isolate vaccine against challenge of homologous and heterologous strains. ., Supar; Setiadi, Yudi; ., Djaenuri; Kurniasih, Nina; Poerwadhikarta, Bhakti; ., Sjafei
Indonesian Journal of Animal and Veterinary Sciences Vol 6, No 1 (2001)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (153.899 KB) | DOI: 10.14334/jitv.v6i1.220

Abstract

Pasteurella multocida locally isolated from chicken and ducks (BCC 299, BCC 2331, DY1, DY2, 12TG, 15TG) andimported strains (BCC 1359, 1362; HEDDLESTON group 1 and 6 respectively) had been tested for its pathogenicity in theprevious study. The aims of this experiment were to study the preparation of local isolate pasteurellosis vaccines and to determine the protective effect of that vaccines in chicken against the highly pathogenic local isolates of P. multocida. Killed monovalent, bivalent and polyvalent pasteurellosis vaccines were prepared and each was adjunvanted with aluminum hydroxide gel at a final concentration of 1.5% and the cell concentration was equal to the No 10 of MacFarland tube standard. Each of the vaccine prepared was used to vaccinated on a group of six week old of layer chicken (8 per group). Each chicken was subcutaneously injected with 0.2 ml of vaccine, four weeks later each was boostered with similar vaccine with the same dose. Two weeks after giving the boostered vaccine each group of chicken were challenged, half with life bacterium of P. Multocida BCC 2331 and other with DY2. Any chick which survive after challenge was designated as protected by vaccination. Before vaccination 1 ml of blood was drawn from each of chicken and then two weeks apart up to challenge. Serum from each sample was separated and kept in deep freeze until tested by enzyme-linked immunosorbent assay (ELISA). Chicken vaccinated with killed whole cell P. multocida vaccines of monovalent (BCC 2331 or DY2) and bivalent (BCC 2331 + DY2) were protected against challenge with live bacterium of either BCC 2331 or DY2 at rate 67-100%. There was no protection in chicken vaccinated with either BCC 299, DY1, 12TG, 15TG, BCC 1359, or 1362 killed vaccine. Similarly no protection of chicken vaccinated with either DY1 + BCC299, 12TG + 15TG or BCC 1359 + BCC 1362 bivalent vaccines. The protection rate of the polyvalent local isolate vaccine was at average 50-75%. All vaccinated chicken showed the presence of antibody responses againsted the extract cell and whole cell antigens of either P. multocida BCC 2331 or DY2 local isolate as detected by ELISA. The antibody responses from vaccinated chicken against extra cellular antigens prepared from broth cultures of BCC 2331 and DY2 were detected only from vaccinated chicken with vaccine containing killed antigen of BCC 2331 and/or DY2 isolate. It is likely, the local isolate of P. multocida BCC 2331 and DY2 would be benefit for producing inactive fowl cholera vaccine use in Indonesia, but the protective antigen that enhances immune protection should be determined by means of immunoblotting techniques.   Key words: Pasteurella multocida, fowl cholera, vaccine, protection
The development of fowl cholera vaccine: II. Pathogenicity and vaccine protection of Pasteurella multocida local isolates in experimental ducks ., Supar; Setiadi, Yudi; ., Djaenuri; Kurniasih, Nina; Poerwadhikarta, B; ., Sjafei
Indonesian Journal of Animal and Veterinary Sciences Vol 6, No 2 (2001)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (141.931 KB) | DOI: 10.14334/jitv.v6i2.228

Abstract

Pasteurellosis or fowl cholera in ducks occurs sporadically along the year in many high duck population areas of Java and other parts of Indonesia. Some isolates of Pasteurella multocida from ducks and chicken are kept at the BALITVET culture collection. The aims of this research were to evaluate the pathogenicity of local isolates and imported strains of P. multocida and to study the pasteurellosis local isolate vaccine and protection assay in ducks. Two imported strains of P. multocida (BCC 1359, BCC 1362) and 6 local isolates (BCC 299, BCC 2331, DY1, DY2, 12TG, 15TG) were used in this study. In the pathogenicity assays the imported strains and local isolates were activated in mice and in duct and then in brain hearth infusion broth containing 5% normal sheep serum. Each of broth culture was diluted, each dilution (102 and 104) of strains or isolates was injected intraperitoneally into a group of normal ducks. Antigen for vaccine, each was produced in sheep blood (5%) agar. Cells were harvested and killed with 0.1% formalin. Monovalent, bivalent, and polyvalent vaccines were prepared, at concentration equal to the Macfarland standard tube No 10, and each was adjuvanted with alhydrogel at final concentration of 1.5%. Each vaccine type was injected into a group of 10 week old ducks (8 animals per group), with 0.2 ml/injection. Four weeks later each animal in group were boostered with the same vaccine, dose, route as the previous injection. Before vaccination each animal was bleed through wing vena, then every two weeks, serum was collected and stored at -20oC. Two weeks after boostered, three days after the last blood sample collection, half animal of each group were challenged intraperitonelly with the BCC 2331 and half with DY2 live broth culture. The pathogenicity assays showed the only BCC 2331 and DY2 killed the experimental ducks, the other did not. The animals vaccinated with either BCC 2331,  DY2 or bivalent (BCC 2331+DY2) vaccines were protected with either life bacterial challenged of either BCC 2331 or DY2 local isolates. It is likely, P. multocida BCC 2331 and DY2 isolates can be used for pasteurellosis candidate vaccine used in Indonesia, but it still needs more studies in the immunological of protective antigens.   Key words: Pasteurella multocida, fowl cholera, ducks, vaccine, protection
The pathogenesis of Pasteurella multocida local isolates in mice and chicken ., Supar; Setiadi, Yudi; ., Djaenuri; Kurniasih, Nina; Poerwadhikarta, Bhakti
Indonesian Journal of Animal and Veterinary Sciences Vol 5, No 1 (2000)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (153.361 KB) | DOI: 10.14334/jitv.v5i1.180

Abstract

Avian cholera or fowl cholera is a bacterial disease caused by Pasteurella multocida strain of serogroup A, has been recognized as important disease in domestic poultry such as ducks and chicken. P. multocida strains derived from overseas and local isolates are stored as freeze dried and kept at the Research hlstitute for Veterinary Science (BALITVET) culture collection (BCC). Some of those bacteria are still alive and can be used as vaccine candidates. Each strain or isolate was activated in brain heart infusion broth containing foetal calf serum and incubated at 37°C then it was identitied by biochemical reactions. Field surveys Were conducted in Central Java and South Kalimantan to observe fowl cholera problems and sample collections for isolation of pathogens. Of the 14 of Pasteurella multocida strains or isolates from BCC, II strains (9 imported 2 local isolates) were still alive. In addition to this 2 isolates trom chicken and duck were viable. Seven out of 9 imported strains killed mice within 3 x 24 hours, similarly for the local isolates (BCC 299, 2331, DYI, DY2, 12TG, 15TG). However, the only BCC 2331 and DY2 isolates were able to kill two week old chicken witIlin 6 x 24 hours post inoculation. From this experiment indicated that the P. multocida local isolates (BCC 2,331 and DY2) are more pathogenic than that of imported strains. Two strains of imported P. multocida BCC 2331, 1362 and 6 local isolates (BCC 299, 2331, DYI, DY2, 12TG and 15TG would be selected for mono- and polyvalent vaccine candidates in the following experiments and the highly patogenic BCC 2331 and DY2 isolates would be used to challenge the vaccinated animals.   Key words: Pasteurella multocida local isolate, pathogenicity, vaccine against fowl cholera candidate
Kaligrafi Al-Quran Sebagai Ornamen Masjid (Studi Living Quran di Masjid Nurul Imam) Setiadi, Yudi
HERMENEUTIK Vol 13, No 2 (2019): Hermeneutik: Jurnal Ilmu al-Qur'an dan Tafsir
Publisher : Program Studi Ilmu Al-Qur`an dan Tafsir, Fakultas Ushuluddin, IAIN Kudus

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21043/hermeneutik.v13i2.6404

Abstract

This study discusses the practice of using the verses of the QurÂ’an as ornamental mosques. This research is known as Living QurÂ’an. This study focuses more on the practical role of the Qur'an. In this case, the Qur'an not only serves as a guide. Al-Qur'an is used as decoration of the mosque in the form of calligraphy. This study uses a qualitative research method with the phenomenological approach of Alfred Schutz, in-order-to motive and because motive to uncover the reasons and objectives of making al-Qur'an calligraphy. The data of this study came from the results of observations, interviews, and documentation. This research found several findings. First, al-Qur'an is not only a guide, but transformed into decorating the mosque in the form of calligraphy. Second, the use of al-Qur'an verses as mosque ornaments indicates the mosque administrators' understanding and thought of the Qur'an. Third, because the motive for making calligraphy is the educational background of the founder of the pesantren mosque and the condition of the mosque congregation. Fourth, in-order-to motive calligraphy making is a trigger for the congregation to learn to read the Qur'an, the message of preaching, and for the congregation to read the Qur'an when visiting the mosque.
Performance Testing of Cross Wind Turbine Model with Capacity of 10 W Achdi, Endang; Sonawan, Hery; Setiadi, Yudi
Mestro: Jurnal Teknik Mesin dan Elektro Vol 6 No 01 (2024): Edisi Juni
Publisher : Fakultas Teknik Universitas 17 Agustus 1945 Cirebon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47685/mestro.v6i01.527

Abstract

Wind is a significant potential renewable energy source for electricity generation. Currently, there are two commonly used types of wind turbines: horizontal-axis and vertical-axis wind turbines. Both types are designed to harness the energy from horizontally moving wind. However, in reality, wind often moves erratically, especially in urban areas with tall buildings. To address this, a cross-axis wind turbine model has been developed to capture energy from randomly moving wind. This turbine model has 10 horizontal blades and 5 vertical blades. Previously, performance testing of this wind turbine model used a 60 W capacity dynamometer generator, but the wind turbine's output power didn't reach the required high torque and rotational speed. In this research, a 40 W capacity generator was used to resolve the previous issues. The parameters measured in this testing include wind speed, turbine rotational speed, voltage, and current. The testing was conducted at wind speeds of 5.3 m/s, 5.8 m/s, 6.3 m/s, and 6.8 m/s, with variations in the blade pitch angles set at 20°, 25°, and 30°. The results of the testing and analysis showed that the maximum performance coefficient of this wind turbine was 7.54%, achieved at a wind speed of 6.3 m/s with a blade pitch angle of 25° and a tip speed ratio of 1,08. The higher the wind speed, the shorter the time required to reach a constant rotational speed. At a wind speed of 6.3 m/s with a blade pitch angle of 25°, it took 30 seconds to reach a constant rotational speed.
Co-Authors Achdi, Endang B Poerwadhikarta Bhakti Poerwadhikarta Djaenuri . Hery Sonawan Nina Kurniasih Sjafei . Supar .