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All Journal Saintika Medika: Jurnal Ilmu Kesehatan dan Kedokteran Keluarga. Farmasains : Jurnal Farmasi dan Ilmu Kesehatan Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Tumbuhan Obat Indonesia Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice) Pharmaciana: Jurnal Kefarmasian Jurnal Kesehatan Masyarakat (JKM) CENDEKIA UTAMA Indonesian Journal of Biotechnology Majalah Obat Tradisional Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) Caraka Tani: Journal of Sustainable Agriculture Jurnal Biologi Tropis JMM (Jurnal Masyarakat Mandiri) PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) Jurnal Kreativitas PKM Medical Sains : Jurnal Ilmiah Kefarmasian Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice)
Siti Rofida
Universitas Muhammadiyah Malang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Other

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THE APOPTOSIS INDUCTION OF Zingiber oficinale ETHANOLIC EXTRACT-Treated HeLa (HUMAN CERVICAL CANCER) CELLS AND ACTIVE COMPOUND PROFILING USING GAS CHROMATOGRAPHY/MASS SPECTROMETRY Laela Hayu Nurani; Siti Rofida; Dwi Utami; Citra Ariani Edityaningrum; Any Guntarti
Medical Sains : Jurnal Ilmiah Kefarmasian Vol 9 No 1 (2024)
Publisher : Sekolah Tinggi Farmasi Muhammadiyah Cirebon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37874/ms.v9i1.1059

Abstract

Cervical cancer accounts for the highest percentage of cancer-related deaths in Indonesia, accounting for nearly 60% of all cancer cases.  Therefore, research into the anticancer mechanisms needs to be conducted.  The ethanolic extract of Zingiber officinale (EEZO) contains zingiberene, a chemical known for its anticancer activity. Understanding the mechanism underlying the apoptosis-inducing effects is crucial. This study aimed to elucidate the apoptotic pathway and analyze the gas chromatography/mass spectrometry (GC/MS) profile of EEZO cells. The research commenced with the maceration of Zingiber officinale rhizomes using 75% ethanol to obtain EEZO. Apoptosis assays were conducted on both a negative control group and an EEZO-treated group of HeLa cells (cervical cancer cells). The apoptotic mechanism was evaluated using forward scattered light-side scattered light (FSC-SSC), fluorescein isothiocyanate (FTIC), and phycoerythrin (PE) flow cytometry. Apoptotic results were analyzed by comparing the control and EEZO samples, which revealed the number of viable cells, apoptotic cells, and cells in the sub-G1 phase. The major constituent of EEZO, which is expected to be a potent apoptosis inducer, was detected using GC/MS. The FSC-SSC results indicated a lower number of viable cells in the EEZO-exposed group than in the control group. FTIC results demonstrated that EEZO significantly increased apoptotic cell death, increasing from 68 to 1537 cells. PE flow cytometry revealed an elevated sub-G1 cell population, indicating the induction of apoptosis by EEZO. GC/MS analysis revealed five dominant components in EEZO, which had the potential to induce apoptosis: L-borneol, zingiberene, farnesol, beta-sesquiphellandrene, and alpha-curcumene ...  
Analisis Jejaring Farmakologi Metabolit Sekunder Tanaman Alang-Alang (Imperata cylindrica) Terhadap Penyakit Stroke Iskemik Syabana, Nada Laela; Rofida, Siti; Jamil, Ahmad Shobrun
Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) Vol 11 No 1 (2025): Agustus 2025
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/ejbst.v11i1.601

Abstract

Stroke iskemik disebabkan oleh adanya plak aterosklerosis sehingga aliran darah menuju otak tersumbat. Hal ini terjadi karena adanya faktor risiko, yaitu hipertensi, diabetes melitus, dan kolesterol. Untuk mencegahnya, dilakukan penelitian menggunakan tanaman Imperata cylindrica yang sudah terbukti memiliki efek antihipertensi, antikolesterol, dan antidiabetes pada penelitian sebelumnya. Metode penelitian yang digunakan yaitu jejaring farmakologi menggunakan cytoscape dan analisis komputasi menggunakan molecular docking. Dilakukan analisis penunjang seperti, evaluasi farmakokinetik obat, kemiripan obat, prediksi dan validasi target. Didapatkan sebanyak 59 protein gen memiliki keterkaitan dengan tanaman Imperata cylindrica dan penyakit stroke iskemik dengan PTGS2 menjadi protein paling berpengaruh karena terhubung dengan hampir seluruh jalur persinyalan, seperti jalur metabolisme, resistensi insulin, AGE-RAGE pada komplikasi diabetes, biosintesis hormon steroid, dan kardiomiopati diabetes.
Cytotoxic Potential of Combination of Hibiscus sabdariffa L. Extract and Cisplatin against HeLa Cervical Cancer Cell Line: A Study of Antiproliferative Activity and Apoptosis Induction Nurani, Laela Hayu; Azzahra, Fara; Utami, Dwi; Edityaningrum, Citra Ariani; Guntarti, Any; Irham, Lalu Muhammad; Daud, Intan; Khairurrizki, Amanda; Putra, Ichsan Luqmana Indra; Ismiyati, Nur; Rofida, Siti; Sugiyanto, Sugiyanto
Majalah Obat Tradisional Vol 30, No 2 (2025)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.92440

Abstract

Cervical cancer is one of the most prevalent cancer types, making the development of effective anticancer agents critical. Cisplatin (CIS) is a drug that has been used to treat cancer, but it also affects normal cells. Hibiscus sabdariffa L. extract (HSE), which has the potential as an anticancer agent, can be developed as a co-chemotherapy with CIS. This study aimed to determine the potential of HSE as a co-chemotherapy with CIS against HeLa cervical cancer cells and determine specific and non-specific parameters of the studied extract. Hibiscus sabdariffa L. (HS) simplicia powder was macerated with 96% ethanol. An in vitro evaluation was carried out on antiproliferative activity by calculating the doubling time. Additionally, a selectivity test was conducted to calculate combination index (CI) values based on the microtetrazolium (MTT) method. Apoptosis mechanisms were explored based on immunocytochemical methods using p53, caspase, and Bax antibodies, followed by an observation of apoptotic induction using a flow cytometer. The HSE and CIS selectivity index values obtained for Vero cells and HeLa cells were 209 and 278, respectively. The antiproliferation test results showed that the combination of HSE and CIS could better extend the doubling time of cells compared to the negative control. This combination also demonstrated a strong synergistic effect, with a CI value of 0.001. The extract as a co-chemotherapy with CIS was capable of increasing the expression of p53, caspase-3, and Bax. The flow cytometry analysis results indicated that HSE could induce apoptosis. Based on the results on the IC50 and CI value of HSE, as well as on the doubling time and apoptosis induction of HSE-influenced HeLa cells, it is concluded that HSE has the potential as a co-chemotherapy against cervical cancer.
Aktivitas Antihiperlipidemia Ekstrak Etanol Daun Annona squamosa L. Rofida, Siti; Firdiansyah, Ahmad; Fitriyastuti, Endah
Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice) Vol. 2 No. 1 (2015)
Publisher : Faculty of Pharmacy, Widya Mandala Surabaya Catholic University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jfst.v2i1.693

Abstract

Cause of cardiovascular disease risk factors, one of which is a high cholesterol levels in serum. Mortality caused by cardiovascular disease as 17.3 million people. Annona squamosa L. or commonly referred custard apple is a plant from family Annonaceae and have been used for insecticide, antitumor, antidiabetic, antioxidant, anti-inflammatory, and antihyperlipidemia. The leaves of Annona squamosa L. contains acetogenin, diterpene, flavonoids, lignin, hydroxyl ketones, and benzoquinazolin. The aims of this study is to determine the optimum dose to reduce levels of LDL on hyperlipidemic rats. In this study, hyperlipidemic rats were divided into 5 groups, negative control group (normal feed), positive control group (normal feed and simvastatin at a dose of 0.9 mg/KgBW), treatment group 1 (normal feed and ethanol extract leaves of Annona squamosa L. at a dose of 0.25 mg/gBB, treatment groups 2 (normal feed and ethanol extract leaf of Annona squamosa L. at a dose of 0.75 mg/gBB, treatment groups 3 (normal feed and ethanol extract leaf of Annona squamosa L. at a dose of 1 mg/gBB). The results of identification by thin-layer chromatography on ethanol extract leaf of Annona squamosa L indicates the presence of flavonoids. The results showed that the ethanol extract leaves of Annona squamosa Linn. On hyperlipidemia rats, can lower LDL cholesterol levels at doses of 0.25 mg/gram BB.
Co-Authors Ahmad Shobrun Jamil Amaliyah Dina Anggraeni Anak Agung Istri Sri Wiadnyani Any Guntarti Any Guntarti Aufa Fadnurrahim Azzahra, Fara Citra Ariani Edityaningrum, Citra Daud, Intan Dian Yuliartha Lestari Dinda Farida Dwi Hartanti Dwi Retno Nur Syahida Erfan Dani Septia Firdiansyah, Ahmad Firzannida Firzannida Fitriyastuti, Endah Ichsan Luqmana Indra Putra, Ichsan Luqmana Indra Ilham Niawan Saputra Khairurrizki, Amanda Laela Hayu Nurani Lalu Muhammad Irham Lely Mauliana Lilik Yusetyani Lilik Yusetyani Maftuchah Maftuchah Mohammad Saifur Rahman Moonike Era Wahyuningati Muhammad Yusron Maulana El-Yunusi Nur Ismiyati Nurul Amalia Azhari Nurwahdaniati Nurwahdaniati Nurwahdaniati Nurwahdaniati, Nurwahdaniati Rahman, Mohammad Saifur Refie Putri Madiyanti Sakti Bagaskara Saputra, Ilham Niawan Savana Sonia Savira Setyawati Soeharto Setyawati Soeharto, Setyawati Sofyan Arief Solly Aryza Sugiyanto - Syabana, Nada Laela Trimianti Hidahyatun Nazah Ulfiatul Jannah Winnugroho Wiratman, Manfaluthy Hakim, Tiara Aninditha, Aru W. Sudoyo, Joedo Prihartono Yustika Faradhiba Yustika Savira