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All Journal Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Indonesian Journal of Urology Mutiara Medika: Jurnal Kedokteran dan Kesehatan Cermin Dunia Kedokteran
Marsetyawan HNE Soesatyo
Bagian Histologi dan Biologi Sel, Fakultas Kedokteran, Universitas Gadjah Mada, Yogyakarta, Indonesia
Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health

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Perbandingan Efek Antiinflamasi antara Ekstrak Pare (M. Charantia) dengan Kortikosteroid terhadap Dermatitis Eksperimental pada Mencit secara Topikal Jenie, Ikhlas Muhammad; Soesatyo, Marsetyawan HNE; -, Praseno
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 3, No 2 (2003)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/mmjkk.v3i2.1700

Abstract

SARS (severe acute respiratory syndrome) represents how confusing the picture of inflammation is. In spite of its ability to protect our body, inflamma¬tion could have some disadvantages. Corticosteroid is one of symtomatically therapy for SARS. Corticosteroids itself has been used for a long time as antiinflammatory drug. But, its adverse reaction such immunocomprimised effect has limited its widely use. It was reported that a and b momorcharin from Pare has antiinflammatory activity. Momorcharin has an ability to prevent antigen-induced limfosit pro¬liferation and reduce the ability of macrofag to fagosit antigen. The aim of this research is to know how good the antiinflammatory effect of Pare comparing with hidrocortison’s is. The subject of this observation are mice as many as 22, which randomly divided into 4 groups. Each group had been injected with S. aureus subcutan. As soon as signs og infection appeared, we treated one group with Pare oint¬ment, one other group with hidrocortison, and 2 groups the rest as positive and negative control. The inflammation reaction in each group had been recorded, clinically and histologically. The datas were assessed with descriptively and qualitatifly analitic. The result was Pare itself had antiinflammatory effect. Topically, it could make inflammation reaction reduced, udema diminished, necrose not widened, healing time shorted and fibrosis prevented. But, still, its effect was not as strong as corticosteroid had. Neverthanless, all mice that had been treated with corticosteroid had died because of sepsis or immunocomprimised state, that Pare wouldn’t had.Fenomena SARS (severe acute respiratory syndrome) mewakili gambaran hebatnya reaksi inflamasi, yang merupakan respon imun bermata dua. Pada satu sisi merupakan alat pertahanan tubuh, namun pada sisi yang lain dapat merugikan. Terapi simtomatis SARS salah satunya adalah pemberian metilprednisolon, yang merupakan golongan kortikosteroid. Sebagai antiinflamasi, kortikosteroid sudah digunakan setengah abad lamanya. Penggunaan kortikosteroid sebagai antiinflamasi dibatasi oleh efek samping yang ditimbulkannya, terutama penurunan status imun/ imunokompromised. Dilaporkan bahwa biji pare (M charantia) -mengandung zat a dan b momorcharin- mempunyai aktivitas antiinflamasi. In vitro momorcharin mampu menghambat proliferasi limfosit terinduksi antigen dan menurunkan fungsi fagosit dari makrofag. In vivo pada tikus mampu menghambat migrasi makrofag dan reaksi hipersensitifitas tipe lambat. Penelitian ini bertujuan untuk membandingkan efek antiinflamasi antara ekstrak Pare 10% dengan hidrokortison 1% secara topikal terhadap dermatitis eksperimental pada mencit. Subyek penelitian adalah mencit berjumlah 22 ekor, dibagi random menjadi empat kelompok: K (6 ekor), C (7 ekor) dan P (7 ekor,) masing-masing mendapat injeksi subkutan S. aureus 9 x 10,0ul. Terjadi infeksi lokal dengan angka kegagalan 5%. Mencit kelompok C mendapat terapi ointment Hidrokortison 1%, kelompok P oint¬ment ekstrak biji pare 10%, kelompok K tidak mendapat terap i/kontrol positif dan kelompok S (2 ekor) kontrol negatif. Pengukuran meliputi derajat peradangan -secara klinis dan histopatologi- serta kesembuhan yang dicapai. Analitisa data bersifat kualitatif deskriptif. Didapatkan hasil ekstrak biji pare mempunyai efek antiinflamasi. Pemberiannya secara topikal mampu memperlambat reaksi peradangan, mencegah akumulasi sel radang, meminimalisasi udema, mengurangi nekrosis, waktu pemulihan lebih cepat dan menghambat terbentuknya jaringan parut. Tetapi, efek antiinflamasi ekstrak biji pare masih lebih rendah dibandingkan dengan hidrokortison. Meskipun demikian seluruh mencit kelompok C/terapi hidrokortison mati dalam kondisi imunokompromised/sepsis.
Correlation between micro-RNA-21 expression and inflammation cytokine in rabbits implanted with bare metal stent with the incidence of neo intimal hyperplasia Hariawan, Hariadi; Soesatyo, Marsetyawan HNE; Munawar, Muhammad; Ghozali, Ahmad
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 51, No 1 (2019)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (377.065 KB) | DOI: 10.19106/JMedScie/005101201901

Abstract

In-stent restenosis after stenting in vascular occurs secondary to the accumulation of smooth muscle cell and extracellular matrix. This condition is a major complication caused by the occurrence of neointimal hyperplasia (NIH). The study aimed to prove the role of miRNA-21 as a risk predictor of the NIH event in aorta of rabbits animal model who underwent bare metal (BM) type stent implantation and to know the miRNA-21 role in IL-6 and IL-8 expressions. This study was quasi experimental, coducted in Catheterization Laboratory Dr. Sardjito General Hospital Yogyakarta. Stent implantation was observed intravascular ultrasonography. Blood vessel which was already deployed by stent taken as sample for pathologic examination. Rabbit blood samples were collected on day 0, 7, and 28. Quantification miRNA-21 expression has been done with qPCR and ELISA approach. These 10 rabbits divided into two groups in which one group as control (without stent deployment, 4 models) and another group as intervention (with BM type stent deployment, 6 models). The increase in expression of miRNA-21 on the day 7 and day 28 in the intervention group compared to the control group. Neointimal hyperplasia increased in intervention group on day 7, and 28 were from mild grade to moderate, and severe respectively. In addition, levels of IL-6 and IL-8 increased on day 28 compared with on day 7. This study showed increase of miRNA-21 expression on day 7 and NIH rise from the mild to moderate. Furthermore, on day 28, that increases of miRNA-21 expression and NIH rose from to moderate to severe. The increase of miRNA-21 was also shown on day 7 and 28 followed by the increase of IL-6 and IL-8 levels.
Moderate-intensity interval exercise but not high-intensity interval exercise improves the spatial memory of ovariectomized rats Kaidah, Siti; Rauf, Saidah; Soesatyo, Marsetyawan HNE; Agustiningsih, Denny; Partadiredja, Ginus
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 50, No 3 (2018)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (518.401 KB) | DOI: 10.19106/JMedScie/005003201804

Abstract

Physical exercise exerts beneficial effects on the spatial learning and memory. High-intensity interval exercise (HIIE) has been proposed as a time-efficient physical exercise regimen. On the other hand, there were evidences that HIIE increased oxidative stress biomarkers and reduced antioxidant capacity, which resulted in oxidative damage. The present study aimed to investigate the effects of high-intensity interval exercise and moderate-intensity interval exercise on oxidative stress biomarkers and oxidative enzymes activity in the hippocampus and the spatial memory of ovariectomized rats. A total of 16 female Sprague Dawley rats aged 12 weeks were randomly assigned into 4 groups, i.e. the sham-operated (SO), ovariectomized without exercise (O), ovariectomized with high-intensity interval exercise (HIIE), and ovariectomized with moderate-intensity interval exercise (MIIE) groups. Rats of the exercise groups (HIIE & MIIE groups) performed 6 sessions of interval exercise per week for 6 weeks. The spatial memory of rats was measured using the Morris water maze procedure. The malondialdehyde (MDA) levels and activity of catalase (Cat) as well as glutathione peroxidase (GPx) in hippocampus were determined using spectrophotometry method. The spatial learning and memory retention of the moderate-intensity interval exercise group was significantly better than that of the high-intensity interval exercise group. The GPx activity of MIIE group was higher than any other groups. The SO group had the lowest hippocampal MDA level and highest Cat activity among groups. Moderate-intensity interval exercise reduces the ovariectomy induced-oxidative stress in the hippocampus and improves spatial learning and memory retention of ovariectomized rats.
THE IGF-1 LEVEL OF ESRD PATIENTS AND ITS RISK FACTORS Hidayati, Titiek; Yuningtyaswari, Yuningtyaswari; Sadewa, Ahmad Hamim; Soesatyo, Marsetyawan HNE
Indonesian Journal of Urology Vol 21 No 1 (2014)
Publisher : Indonesian Urological Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32421/juri.v21i1.24

Abstract

Objective: To identify the Insulin-like Growth Factor–1 (IGF-1) level of End Stage Renal Disease (ESRD) and non ESRD populations, and correlation between IGF-1level and ESRD incidences. Material & Method: This case study was carried out in Yogyakarta with 72 volunteers. The cases involved Chronic Kidney Disease (CKD) patients. The controls were non-CKD patients. CKD parameters were established with PERNEFRI diagnostic criteria. Comparison of IGF-1 levels between case and control groups was performed through ANOVA, with confidence level of 95%. Bivariate analysis to identify the correlation between IGF-1 plasma level, smoking status, illness history and body mass index (BMI) by determining odds ratio (OR) of individual risk factor of p < 0.05. Results: We enrolled 72 volunteers, 45 male and 27 female subjects. Of the 45 male patients, 15 CKD and 30 non CKD patients served as cases and controls, respectively. The difference in plasma IGF-1 level was detected in the case and control groups (42.01 ± 10.66 vs. 56.05 ± 24.91) (p < 0.05). The result of bivariate analysis showed passive smoking status, IGF-1 plasma level, DM history and hypertensive illness history had correlation with ESRD incidence with odds ratios of 7.88 (p < 0.005; CI: 1.6-37.5) for passive smokers, 4.3 (p < 0.05, CI: 1.36 to 13.33) for IGF-1 level, 21.5 (p < 0.05; CI) for DM history and 12.4 (p < 0.05; CI: 3.7 to 41) for hypertensive history. Conclusion: There was difference in IGF-1 plasma level between ESRD and non-ESRD patients. The IGF-1 plasma level, passive smoking status, diabetes history, and hypertensive history have correlation with ESRD incidence.Keywords: Insulin-like Growth Factor–1 level, End Stage Renal Disease, case control, odds ratio.
Kinetika Demam Berdarah Dengue dalam Spektrum Imunopatogenesis dan Klinis Lardo, Soroy; Soesatyo, Marsetyawan HNE; -, Juffrie; Umniyati, Sitti Rahmah
Cermin Dunia Kedokteran Vol 43, No 12 (2016): Kardiovaskular
Publisher : PT. Kalbe Farma Tbk.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (151.299 KB) | DOI: 10.55175/cdk.v43i12.896

Abstract

Virus dengue adalah virus RNA termasuk genus Flavivirus dan familia Flaviviridae. Sampai saat ini dikenal empat serotipe yaitu DEN-1, DEN-2, DEN-3 dan DEN-4. Infeksi salah satu serotipe akan menimbulkan antibodi protektif seumur hidup, tetapi tidak untuk serotipe lain. Struktur virus memiliki 10.700 basa di dalam genomnya. Di dalam genom terdapat sebuah single open reading frame (SORF) yang mengkode dua macam protein yaitu protein struktural dan nonstruktural. Protein NS1 merupakan protein non struktural glikoprotein dan bukan bagian dari struktur virion, tetapi diekspresikan pada permukaan sel. Protein NS1 merupakan penanda awal infeksi dengue yang akan berperan baik untuk proses imunopatogenesis dan spektrum klinis.Dengue virus is a RNA virus, genus Flavivirus and family Flaviviridae. There are currently four known serotypes, namely DEN-1, DEN-2, DEN-3 and DEN-4. Infection of one serotype will produce a lifetime protective antibodies, but not for the other serotypes. The structure of the virus has a base of 10,700 in its genome. There is a single open reading frame (SORF) that encodes two different kinds protein that is stuctural and non structural protein. NS1 protein is a non structural protein glycoprotein, not part of the virion structure, but expressed on the cell surface. The kinetic profile of dengue virus NS1 protein is an early marker of dengue infection which would contribute both to the immunopathogenesis and clinical spectrum.NS1 protein has as important antigenecity as an early marker.
Co-Authors -, Juffrie -, Praseno Ahmad Hamim Sadewa Denny Agustiningsih Ghozali, Ahmad Ginus Partadiredja Hariadi Hariawan Ikhlas Muhammad Jenie Juffrie, Juffrie Muhammad Munawar Saidah Rauf Siti Kaidah Sitti Rahmah Umniyati Soroy Lardo Titiek Suhardi Haripurnomo Kushadiwijaya Hidayati Yuningtyaswari Yuningtyaswari