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All Journal Jurnal Fitopatologi Indonesia Jurnal Agroteknos JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Susamto Somowiyarjo
Fakultas Pertanian Universitas Gadjah Mada, Yogyakarta, Indonesia
Agriculture, Biological Sciences & Forestry Medicine & Pharmacology

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DETECTION AND IDENTIFICATION OF YELLOW MOSAIC STUNT DISEASE ON Petunia sp. USING NESTED PCR METHOD Astuti, Suryani Titi; Sulandari, Sri; Hartono, Sedyo; Somowiyarjo, Susamto
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol. 21 No. 1 (2021): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.12156-62

Abstract

Detection and identification of yellow mosaic stunt disease on Petunia sp. using nested PCR method. Yellow mosaic stuntdisease was found at some nurseries of Petunia in Sleman, Yogyakarta, also in Muntilan and Magelang Central Java. Thedisease was very important due to its ability reducing the quality and quantity of Petunia seedlings. The causal agent of thedisease may be carried over to imported seeds and necessary to identify as a basic information for developing controlstrategies. This research was done by mechanical transmission on indicator plants. The observation of the causal agents wasconducted using electron microscope with quick dipping method and the molecular detection was done using nested PCRwith TobRT up1-TobRT do2 as the external primers and TobN up3-TobN do4 as the internal primers. Mechanical inoculationshowed chlorosis symptoms that developed into local spot on Chenopodium amaranticolor as well as mosaic and veinbanding on Nicotiana benthamiana. The observation using electron microscope showed rod-shaped virus particles sizedapproximately 300 nm and by PCR method produced around 568 bp and 400 bp DNA band. Based on the sequence analysis,the disease was caused by Rehmania mosaic virus. This type of Tobamovirus has 96% similarity with ReMV-Japan. ReMV, aplant pathogen which was a member of Tobamovirus that has never been reported in Indonesia. This research was the firstreport of ReMV in Indonesia infecting Petunia as ornamental plant.
POLA AGIHAN DAN INTENSITAS PENYAKIT BUSUK PANGKAL BATANG LADA DI PROVINSI SULAWESI TENGGARA BANDE, LA ODE SANTIAJI; HADISUTRISNO, BAMBANG; SOMOWIYARJO, SUSAMTO
Jurnal Agroteknos Vol 4, No 1 (2014)
Publisher : Jurnal Agroteknos

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (192.864 KB)

Abstract

The success of foot rot disease control is largely dependent on information data of pepper cultivation conditions, distribution pattern of the disease, and the magnitude of the intensity of the disease. This study aimed to determine the condition of pepper cultivation, distribution pattern of pepper foot rot disease, the development of disease symptoms, and intensity of pepper foot rot disease in Southeast Sulawesi. Data cultivation conditions, distribution of the disease, progression of symptoms, and the intensity of the disease were obtained by means of surveys in pepper plantations and interviews with pepper farmers. The results showed that the pepper plantations in Southeast Sulawesi were cultivated on flat to hilly topography, and the cultivation method was very conventional but herbicide use was very intensive. The development of wilt symptoms on pepper plants was very quick in dry weather but slow in the wet. Pepper foot rot disease has patch distribution. The highest intensity of the pepper foot rot disease was in less weedy plantations with intensive use of herbicides. The intensity of the pepper foot rot disease in Southeast Sulawesi was 61,2% with the spread in each district namely South Konawe at 53,8%, Konawe at 63,7% and Kolaka by 61,2%.
DETEKSI DAN PENGHITUNGAN KERAPATAN INOKULUM Phytophthora capsici DALAM TANAH DENGAN MENGGUNAKAN UMPAN DAUN LADA BANDE, LA ODE SANTIAJI; HADISUTRISNO, BAMBANG; SOMOWIYARJO, SUSAMTO; SUNARMINTO, BAMBANG HENDRO
Jurnal Agroteknos Vol 4, No 3 (2014)
Publisher : Jurnal Agroteknos

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (175.371 KB)

Abstract

Phytophthora capsiciis a causal agent for footrot disease in pepper and classified as a soil-borne pathogen. The inoculums of P. capsici in the soilis difficultto detect. The dynamics of P. capsici population in the soil is frequently and rapidly fluctuates and hard to detect, causing the pathogen to produce disease rapidly. The aimsof this research were todetect the pathogen P.capsici using black pepper leaf baiting and to quantify the inoculum of the pathogen P.capsici in the soil belonging to several disease intensities of the black pepper foot rot in the field. The first experiment: detecting the pathogen P. capsici using black pepper leaf baiting in the soil artificially infested using several sporangia, anda second experiment: quantification of propagul of the P.capsici in various categories of intensity on the black pepper foot rot disease in the field. The research results showed that the black pepper leaf baiting could be used to detect the existence of the propagul of P.capsiciin the soil artificially infested in various densities of sporangia.  The increase in disease intensity occurred in parallel with the greater density of P. capsici inocula in soil. The density of P. capsici inocula in the soil tended to decline when the disease intensity reached the highest level.
Incidence of dwarf disease and yield on Inpari 32 over two sequential seasons in Klaten, Central Java Febrianto, Sudi; Sulandari, Sri; Somowiyarjo, Susamto
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 23 No. 1 (2023): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PE
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.12367-76

Abstract

Rice dwarf disease caused by Rice grassy stunt virus (RGSV) and Rice ragged stunt virus (RRSV) is frequently found in Indonesia. This disease is transmitted by brown planthopper/BPH (Nilaparvata lugens Stål). The infected plants become dwarfs and lead to reduced yields. The purpose of this study is to determine the number of BPH populations due to the occurrences of dwarf disease and yield on Inpari 32 rice over two sequential planting seasons in Klaten, Central Java. The observed parameters were dwarf disease symptoms, incidence and intensity of the disease, BPH population and its natural enemy, the vegetative growth, and yields. The analysis used in this study were descriptive analysis and linear regression. The results showed that, plant growth during the dry season was better than the rainy season based on plant height and tiller number. The average population of BPH in the dry season were 0.2–4 individual/hill which is lower than in the rainy season (0.43–4.4 individual/hill). The incidence and intensity of disease in the dry season were lower than rainy season. The higher incidence and intensity of rice dwarf disease in the rainy season caused a decrease in dry harvest yields and milled dry yields were 10.95% and 10.81%, respectively
Deteksi Odontoglossum ringspot virus pada Anggrek Asli Koleksi Kebun Raya di Indonesia Mahfut, Mahfut; Daryono, Budi Setiadi; Somowiyarjo, Susamto
Jurnal Fitopatologi Indonesia Vol 13 No 1 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (316.108 KB) | DOI: 10.14692/jfi.13.1.1

Abstract

Native orchid is one of Indonesian natural resources which play important role as parental materials in breeding program. Virus infection is one of the limiting factors in the cultivation of orchid. The purpose of this study was to detect Odontoglossum ringspot virus (ORSV) from native orchid. Symptomatic orchids were collected from 5 botanical gardens, i.e. Bogor, Cibodas, Purwodadi, Balikpapan, and Enrekang Botanical Gardens. Detection and identification was conducted by serological method using ORSV specific antisera, followed by RT-PCR and DNA sequencing. The serological test showed that 5 samples gave positive reaction against ORSV antiserum, i.e. Phalaenopsis amboinensis (KRB2) and Phalaenopsis amabilis (KRB12) from Bogor Botanical Garden, Phalaenopsis amabilis (KRP18) and Dendrobium salacence (KRP20) from Purwodadi Botanical Garden, dan Phalaenopsis modesta J. J. Sm. (KRBp5) from Balikpapan Botanical Garden. RT-PCR of the 5 samples using specific primer of ORSV coat protein gene was successfully amplified fragment DNA with size ± 474 bp. Homology analysis of those 5 ORSV isolates showed the highest index similiarity of 99.8% with corresponding sequences from 14 other ORSV isolates. Phylogenetic analysis indicated that ORSV KRB2 and KRP18 isolates was clustered in a separate group far from ORSV isolates in other countries. This is the first report of ORSV infection on native orchids collection from 5 botanical gardens in Indonesia.
Co-Authors Astuti, Suryani Titi Bambang HADISUTRISNO Bambang Hendro Sunarminto BUDI SETIADI DARYONO Febrianto, Sudi La Ode Santiaji Bande Mahfut SEDYO HARTONO SRI SULANDARI