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All Journal JURNAL PHOTON Collaborative Medical Journal (CMJ) Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan
Olvaria Misfa
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Agriculture, Biological Sciences & Forestry Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Physics Public Health

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Journal : Collaborative Medical Journal (CMJ)

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UJI AKTIVITAS ANTIFUNGI BAKTERI ASAM LAKTAT ASAL DADIH TERHADAP Fusarium oxysporum Yudiawati Yudiawati; Olvaria Misfa; Eliya Mursyida; Riski Dwi Utami
Collaborative Medical Journal Vol 5 No 2 (2022): Mei
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/cmj.v5i2.3253

Abstract

Intoksikasi mikotoksin pada manusia disebabkan oleh makanan yang dikonsumsi dan merupakan inhibitor poten terhadap sintesis protein, RNA, dan DNA. Fusarium oxysporum merupakan salah satu spesies penyebab intoksikasi mikotoksin yang ditularkan melalui makanan. Mikroba yang umumnya digunakan untuk eliminasi mikotoksin adalah bakteri asam laktat (BAL) yang bersumber dari makanan fermentasi seperti dadih yang berasal dari fermentasi susu kerbau. Tujuan penelitian ini untuk menguji aktivitas antifungi BAL terhadap pertumbuhan F. oxysporum dan menggunakan desain post-test only with control group. Aktivitas BAL terhadap F. oxysporum diuji menggunakan metode sumuran. Data yang diperoleh dianalisis menggunakan uji One Way ANOVA dan uji Post Hoc Bonferroni. Hasil isolasi BAL dari dadih adalah bakteri Gram positif berbentuk bacil dan coccobaci dengan uji katalase negatif. Diameter zona hambat BAL 1, BAL 2, dan BAL 3 yaitu 9,10 mm, 8,30 mm, 7,33 mm. Hasil uji One Way ANOVA menunjukkan terdapat perbedaan bermakna antara ketiga isolat BAL dalam menghambat pertumbuhan F. oxysporum. Hasil uji Post hoc Bobferroni menunjukkan kontrol positif terdapat perbedaan yang bermakna dengan BAL 1, BAL 2, dan BAL 3. Sedangkan, hasil BAL 1, BAL 2, dan BAL 3 tidak terdapat perbedaan bermakna pada kelompok perlakuan. Hasil menunjukkan bahwa tiga isolat BAL mampu menghambat pertumbuhan Fusarium oxysporum.
UJI AKTIVITAS ANTIFUNGI BAKTERI ASAM LAKTAT ASAL DADIH TERHADAP Aspergillus flavus Olvaria Misfa; Viola Anggraini Asrizal; Eliya Mursyida; Uly Astuti Siregar
Collaborative Medical Journal Vol 6 No 1 (2023): Januari
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/cmj.v6i1.3254

Abstract

Primary gastrointestinal aspergillosis (AGP) is an inflammation of the gastrointestinal tract from the duodenum to the rectum. Aspergillus flavus (A. flavus) is one of the fungi that causes aspergillosis. This infection occurs due to aflatoxin from A. flavus which can cause damage to the gastric mucosa. The use of antifungals for long-term treatment can cause toxicity effects and also resistance, so alternative treatments are needed. Dadih is a fermented product of buffalo milk which contains lactic acid bacteria (LAB). Lactic acid bacteria produce acetic acid, lactic acid, and bacteriocin which can inhibit the growth of the fungus A. flavus. LAB species capable of suppressing the growth of A. flavus were Lactococcus lactis, Lactobacillus casei subsp. pseudoplantarum and Lactobacillus plantarum, and Lactobacillus brevis. To determine and analyze the antifungal activity of lactic acid bacteria from curd against Aspergillus flavus. This study used a post test-only design with a control group. The sample used was LAB isolate from curd, while the test fungus used was A. flavus. This study begins with rejuvenation of LAB isolates, then classification of LAB and pathogens, as well as inhibition test using the well diffusion method. The data obtained were analyzed using One-Way ANOVA test and Bonferroni Post hoc test.: LAB isolates from curd were Gram-positive bacteria in the form of bacilli and cocobasil, with negative catalse test results. The average diameter of the inhibition zones of LAB 1, LAB 2, and LAB against A. flavus were 8.55mm, 10.23mm, and 6.20mm. The results of the ANOVA test showed that there was a significant difference between the three LABs. The results of the Bonferron Post hoc test showed that there was a significant difference between LAB 2 isolates and positive controls against LAB 3. Meanwhile, there was no significant difference between LAB 2 and BAL 3 isolates against LAB 1 isolates. LAB isolate from curd was able to inhibit the growth of the fungus A. flavus.
Co-Authors alfiza rahmanita Eliya Mursyida Eliya Mursyida Mahendra Raissa Almira Riski Dwi Utami Santi Widiasari Uly Astuti Siregar Viola Anggraini Asrizal Yudiawati Yudiawati