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All Journal Dentika Dental Journal PROSIDING SEMINAR NASIONAL CENDEKIAWAN Padjadjaran Journal of Dentistry Molecular and Cellular Biomedical Sciences (MCBS) Journal of Indonesian Dental Association Majalah Patologi Indonesia Jurnal Biomedika dan Kesehatan Jurnal Material Kedokteran Gigi Conservative Dentistry Journal Sinnun Maxillofacial Journal Jurnal Abdimas Kesehatan Terpadu Ebers Papyrus
Janti Sudiono
Department of Oral Pathology Faculty of Dentistry Trisakti University Jakarta 11440
Biochemistry, Genetics & Molecular Biology Dentistry Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health

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Regenerative medicine in dental and oral tissues: Dental pulp mesenchymal stem cell Sudiono, Janti; Oka, Ciptadhi Tri; Djamil, Melanie S; Sandra, Ferry
Padjadjaran Journal of Dentistry Vol 28, No 1 (2016): March 2016
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1160.809 KB) | DOI: 10.24198/pjd.vol28no1.13513

Abstract

Background. Regenerative medicine is a new therapeutic modality using cell, stem cell and tissue engineering technologies. Purpose. To describe the regenerative capacity of dental pulp mesenchymal stem cell. Review. In dentistry, stem cell and tissue engineering technologies develop incredibly and attract great interest, due to the capacity to facilitate innovation in dental material and regeneration of dental and oral tissues. Mesenchymal stem cells derived from dental pulp, periodontal ligament and dental follicle, can be isolated, cultured and differentiated into various cells, so that can be useful for regeneration of dental, nerves, periodontal and bone tissues. Tissue engineering is a technology in reconstructive biology, which utilizes mechanical, cellular, or biological mediators to facilitate regeneration or reconstruction of a particular tissue. The multipotency, high proliferation rates and accessibility, make dental pulp as an attractive source of mesenchymal stem cells for tissue regeneration. Revitalized dental pulp and continued root development is the focus of regenerative endodontic while biological techniques that can restore lost alveolar bone, periodontal ligament, and root cementum is the focus of regenerative periodontic. Conclucion. Dentin-derived morphogens such as BMP are known to be involved in the regulation of odontogenesis. The multipotency and angiogenic capacity of DPSCs as the regenerative capacity of human dentin / pulp complex indicated that dental pulp may contain progenitors that are responsible for dentin repair. The human periodontal ligament is a viable alternative source for possible primitive precursors to be used in stem cell therapy.
Cytotoxicity Effect of Borassus flabellifer L. Seed Coat on Fibroblast Tyatana, Risha Nadira; Sudiono, Janti
Journal of Indonesian Dental Association Vol 4 No 2 (2021): October
Publisher : Indonesian Dental Association

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Abstract

Introduction: Medicinal plants or herbal medicine are increasingly being developed and widely used by the community. Herbal medicine can be used as an alternative for the prevention and treatment of chronic, degenerative and cancer diseases. B. flabellifer L. is one of the palm tree which grow at tropical area such as Indonesia with popular name as ‘lontar’ or ‘siwalan’ B. flabellifer L. (lontar) seed coat was reported to have an antimycotic and antiproliferative activity against neoplastic cells. The use of alternative treatment is considered safe if its toxicity to normal cell is low. However there has been no research on cytotoxicity of B. flabellifer L. seed coat towards a normal cell. The normal cell that can be used for cytotoxicity tests were fibroblast. Objective: The aim of this study was to determine the cytotoxicity effect of B. flabellifer L. seed coat extract towards fibroblast. Method: The extract of B. flabellifer L. seed coat was done by maceration technique using 70% ethanol and then diluted to 50%, 25%, 12.5%, 6.25%, 3.125%, and 1.56% concentrations. The MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] method was performed to evaluate fibroblast viability. The fibroblast were tested using various concentrations of B. flabellifer L. seed coat extract and the control groups consisting positive and negative control that were observed for 24 and 48 hours. The absorbance value was measured by microplate reader at 570 nm. Results: At 24 hours observation, the concentration of 1.56% was classified as mild toxic, the concencrations of 3.125%, 6.25%, 12.5% and 25% were classified as moderate, and the concentrarion of 50% as severe toxic. At 48 hours, the concentrations of 1.56%, 3.125%, 6.25% and 12.5% were classified as moderate while the concentrations of 25% and 50% as severe toxic. Conclusion: B. flabellifer L. seed coat extract has cytotoxicity effect towards fibroblast started from concentration of 1.56%.
Vakuolisasi Sitoplasma Sel Epitel Mukosa Mulut sebagai Parameter Perubahan Seluler pada Perokok Luthfiyah, Syifa; Trisfilha, Pretty; Sudiono, Janti
Sinnun Maxillofacial Journal Vol. 7 No. 02 (2025): Oktober 2025
Publisher : Fakultas Kedokteran Gigi Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/smj.v7i02.191

Abstract

Pendahuluan: Merokok merupakan faktor risiko utama berbagai penyakit dan dapat menyebabkan kematian akibat kandungan zat karsinogenik yang dapat merusak DNA serta menimbulkan efek sitotoksik. Salah satu parameter yang dapat digunakan untuk mendeteksi kerusakan DNA adalah vakuolisasi sitoplasma. Tujuan Penelitian: Menganalisis perbedaan jumlah sel epitel mukosa mulut yang mengalami vakuolisasi sitoplasma antara perokok dan non-perokok, serta membandingkan pengaruh jenis rokok yang digunakan. Bahan dan Metode: Penelitian observasional analitik dengan desain cross-sectional ini melibatkan 60 responden, terdiri atas 30 perokok dan 30 non-perokok. Pemeriksaan sel dilakukan menggunakan sitologi eksfoliatif dengan pewarnaan Papanicolaou. Data dianalisis secara statistik dengan tingkat signifikansi 0,05. Hasil: Terdapat perbedaan bermakna jumlah sel dengan vakuolisasi sitoplasma antara kelompok perokok dan non-perokok (p < 0,05). Namun, tidak ditemukan perbedaan bermakna dalam jumlah sel dengan vakuolisasi sitoplasma antara perokok rokok putih, rokok kretek, rokok elektrik, maupun kombinasi rokok konvensional dan elektrik (p > 0,05). Kesimpulan: Merokok berhubungan dengan peningkatan vakuolisasi sitoplasma pada sel epitel mukosa mulut, yang menandakan adanya kerusakan sel akibat paparan asap rokok. Semua jenis rokok yang digunakan memiliki potensi serupa dalam menimbulkan efek sitotoksisitas pada mukosa mulut.
Efek Antiinflamasi Ekstrak Kulit Tamarillo: Pemeriksaan Kadar IL-6 Tikus Pasca Induksi Karagenin Salsabila, Sasya Anursyah; Sudiono, Janti
Jurnal Biomedika dan Kesehatan Vol 5 No 2 (2022)
Publisher : Fakultas Kedokteran Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/JBiomedKes.2022.v5.75-81

Abstract

BACKGROUND Inflammation is a protective response of the body to injury by releasing pro-inflammatory cytokines; one of them is interleukin-6 (IL-6). The peeled fruit of Tamarillo or terung belanda (Solanum betaceum Cav.) ethanol extract contains flavonoid compounds that have anti-inflammatory potential. This research was conducted to evaluate the anti-inflammatory effect of terung belanda (Solanum betaceum Cav.) peel fruit ethanol extract on carrageenan-induced Wistar strain rat by measuring the IL-6 level. METHOD Tamarillo (Solanum betaceum Cav.) fruit peel was extracted by maceration methods using 70% ethanol as solvent. The anti-inflammatory was evaluated using 30 rats which were divided into five research groups positive control (diclofenac sodium), negative control (NaCl), and Tamarillo (Solanum betaceum Cav.) fruit peel ethanol extract with a dose of 70mg/kg BW, 140mg/kg BW, and 280mg/kg BW. After the treatment, the rats were injected with carrageenan as an inflammatory inducer. Blood samples were taken at 24, 48 and 72 hours to measure the IL-6 level using the ELISA kit. Statistical analysis used one-way ANOVA with a significant difference as 0.05. RESULTSThe lowest IL-6 levels (13.853 pg/ml) were found in 24 hours at the group of 140 mg/kg BW while in 48 hours at the 280 mg/kg BW group and 72 hours at the 70 mg/kg BW group. In 24 hours, the 140 mg/kg BW group showed lower IL-6 levels than the positive control with a significant difference (p=0.001). CONCLUSION Tamarillo (Solanum betaceum Cav.) fruit peel ethanol extract is effective as an anti-inflammatory material, especially for 24 hours with a dose of 140 mg/kg BW. KEYWORDS: Tamarillo, Solanum betaceum Cav., Peel Fruit, Ethanol Extract, Carrageenin, IL-6
Eksprsi CD68 pada Makrofag sebagai Efek Antiinflamasi Ekstrak Etanol Kulit Buah Tamarillo (Solanum betaceum Cav.) (Studi pada Mukosa BukalTikus yang Diinduksi Karagenan) Bindaputri, Jelita Febrilia; Sudiono, Janti
Jurnal Biomedika dan Kesehatan Vol 7 No 1 (2024)
Publisher : Fakultas Kedokteran Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/JBiomedKes.2024.v7.6-16

Abstract

Background Inflammation is one problem in the oral cavity that patients often complain about. Anti-inflammatory drugs are generally used to treat inflammation but these drugs have side effects therefore currently many anti-inflammatory drugs are developed from natural ingredients, one of which is Tamarillo. The flavonoid of Tamarillo fruit peel can inhibit inflammation. Carrageenan is an irritant that is often used as an indicator of inflammation because it has many advantages. Macrophages are innate immune cells that are important in inflammation. Macrophage cluster of differentiation 68 or CD68 expression can be used to detect inflammation activity. The objective of this study is to detect CD68 expression on macrophages as the anti-inflammatory effect of Tamarillo fruit peel (Solanum betaceum Cav.) ethanol extract. Methods This study used biological specimens of in vivo experimental research by observing immunohistochemical preparations of 5 sample groups, diclofenac sodium as a positive control, NaCl as a negative control, and Tamarillo fruit peel extract groups at doses of 70, 140, and 280 mg/kg.BW. Statistical analysis was performed using the one-way ANOVA test. Results There were significant differences (p< 0.05) in CD68 expression between each treatment group on the 24, 48, and 72 hours with the lowest number of expressions on the 72 hours of 280 mg/kg.BW group. Conclusions Ethanol extract of Tamarillo (Solanum betaceum Cav.) fruit peel has an effect on CD68 expression of macrophage cells in the buccal mucosa of carrageenan-induced rats with the most optimal dose of 280 mg/kg.BW on 72 hours.
Characterization of a novel calcium phosphate cement–calcium sulfate hemihydrate–acemannan for vital pulp therapy Bryan Wangidjaja; Widyastuti, Wiena; Elline; Sudiono, Janti
Conservative Dentistry Journal Vol. 15 No. 2 (2025): July-December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v15i2.2025.74-79

Abstract

Background: Dental caries remains as one of the most prevalent oral diseases worldwide, especially in developing nations like Indonesia. Untreated caries may lead to pulp involvement, prompting vital pulp therapy (VPT) to protect pulp vitality. The success of VPT is dependent on the biological and physical properties of the capping materials. Traditional pulp capping materials, such calcium hydroxide Ca(OH)₂ and mineral trioxide aggregate (MTA), show bio-activity but are limited by issues of solubility, manipulation, and bio-compatibility. Consequently, calcium phosphate cement (CPC) and calcium sulfate hemihydrate (CSH) with acemannan (Ace) have been explored as potential alternatives. Purpose: This research aimed to evaluate the potential of a mixture containing CPC-CSH-Ace as a bio-active material for vital pulp therapy. Methods: CPC–CSH–Ace was formulated by combining 70 wt% CPC and 30 wt% CSH with acemannan concentrations of 3 wt%, 5 wt%, and 10 wt%. Calcium hydroxide (Dycal, Dentsply) and MTA (Bio MTA+, Cerkamed) were used as controls. Particle size was measured using a Particle Size Analyzer (Horiba SZ-100), surface morphology and calcium ratio were tested with SEM–EDS, and crystal structure was determined using XRD (Rigaku Miniflex). Data were examined utilizing one-way ANOVA and subsequent post-hoc testing (p < 0.05). Results: CPC–CSH–Ace exhibited smaller particle sizes, smoother surfaces, higher Ca/P ratios, and more defined hydroxyapatite peaks than Ca(OH)₂ and MTA. Conclusion The combination of CPC–CSH-Ace showed significant chemical and physical characteristics and has promise as a new bio-active material for vital pulp therapy.
The Effect of Myrmecodia pendans Ethanol Extract on Blood Vessels Within Pulpitis (Study on Sprague Dawley Rats) Janti Sudiono; Patricia Revana
Majalah Patologi Indonesia Vol. 29 No. 1 (2020): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55816/mpi.v29i1.408

Abstract

Sarang semut (Myrmecodia pendans) is one of traditional herbs used to treat various diseases. Myrmecodia pendanscontain chemical compound flavonoid which has anti inflammation effects. Pulpitis is a dental pulp inflammation thatcan be caused by bacteria invasion, physical trauma or iatrogenic factor which affecting blood vessels in dental pulp.This research is an in vivo laboratory experimental pre and post treatment with control. The purpose of this study is tofind out the effects of Myrmecodia pendans ethanol extract on blood vessels appearance of Sprague Dawley rats’dental pulps which had been inducted with 0,01ml Porphyromonas gingivalis for 48 hours. The subjects were dividedinto 3 groups with Group I representing the negative control, group II representing treated group with Myrmecodiapendans ethanol extract, and group III representing positive control with Ca(OH)2. Group II and III were divided intosubgroups based on the treatment periods of 48, 96, 168, 336 hours. The statistic results showed that there weresignificant differences (p<0.05) among the pulpitis, negative control, positive control Ca(OH)2 and the Myrmecodiapendans treatment subgroups of 48, 96, 168, 336 hours. Myrmecodia pendans ethanol extract has comparable antiinflammation effect with Ca(OH)2 which is used to pulpitis commercial medicament.
Anti-inflammatory Effect of Tamarillo (Solanum betaceum Cav.) Fruit Peel Ethanol Extract: Study of Carrageenan-Induced Buccal Mucosa on Rats Syarafina, Amiretta; Sudiono, Janti
Majalah Patologi Indonesia Vol. 32 No. 2, Mei 2023
Publisher : Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55816/mpi.v32i2.620

Abstract

Background Inflammation is a local protective response by tissue damage. Tamarillo fruit peel ethanol extract has flavonoid as anti-inflammatory, anti-oxidant, inhibit cyclooxygenase and lipoxygenase as well as leukocyte accumulation. This study aims to determine anti-inflammatory effect of Tamarillo fruit peel ethanol extract on carrageenan-induced buccal mucosa of rats. Methods Extraction was done by maceration with 70% ethanol. The 30 rats divided into five groups, namely positive control (diclofenac sodium), negative control (NaCl), and extract doses of 70, 140 and 280 mg/kgBW. Carrageenan was injected as inflammation inducer in the right buccal mucosa. Macroscopic and microscopic observations were performed at three times, 24, 48, and 72 hours after carrageenan injection. Results At 24 hours, inflammatory cells of 70 mg/kg extract were 37, at 140 and 280 mg/kgBW were 18; positive and negative control as 35 and 405. At 48 hours, inflammatory cells of positive control were 22; extract of 70, 140, and 280 mg/kgBW were 21, 17, and 16 while negative control was 26. At 72 hours, positive and negative controls were 18 and 27. Extract of 70, 140, and 280 mg/kgBW had 20, 12 and 9 cells. Extract groups had no significant differences with the positive control (p>0.05) except for the 48th hours of 70 mg/kgBW however the extract groups showed significance with negative control (p<0.05). The lowest number cells (6) was at 280 mg/kgBW on 72 hours. Conclusion Tamarillo fruit peel ethanol extract is effective anti-inflammatory agent especially dose of 280 mg/kgBW on 72 hours.
Efek Anti Toksik Ekstrak Etanol Kulit Buah Terung Belanda (Studi Pada Ginjal Tikus Wistar Paska Induksi Karagenin) Agita, Debi; Sudiono, Janti; Trisfilha, Trisfilha
Jurnal Material Kedokteran Gigi Vol 12 No 1 (2023): JMKG Vol 12 No 1 Maret 2023
Publisher : Ikatan Peminat Ilmu Material dan Alat Kedokteran Gigi (IPAMAGI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32793/jmkg.v12i1.776

Abstract

Dutch eggplant fruit is traditional medicine, their skin is often wasted because not many people know what it contains. Objective. To know the anti-toxic effect of dutch eggplant peel’s extract post-carrageenin induction on rat’s kidneys. In vivo experiments was divided into 5 groups, negative control group (saline), positive control group (sodium dilcofenac) 7mg/kgBW, and dutch eggplant’s peel extract at doses of 70mg/kgBW, 140mg/kgBW, and 280mg/kgBW. Mice’s buccal submucosa was injected with Caraagenin 1%. After injection, this study did macroscopic and microscopic observations for 24 hours, 48 hours and 72 hours. Results, Dutch eggplant peel’s phytochemical test contains phenolic, flavonoid, tannin, and alkaloid content. At all doses, anti-toxic effect of dutch eggplant peel’s ethanol extract has same healing effectiveness process with 48 hours. In positive control, the kidneys were normal at 48 hours. Meanwhile, the negative control’s healing effectiveness was seen at 72 hours. The anti-toxic effect from dutch eggplant peel’s ethanol extract has effective healing process at all doses in 48 hours post-carrageenin induction, and did not affect rats’ body weight, however with lower intensity than positive control which using dilcofenac, anti-inflammatory drug.
Co-Authors Agita, Debi Akrom, Akrom Angelina Angelina Arief Budiman, Johan Audry Ariefin, Sariany Pancasari Barnabas Bonardo Hana Christina Bindaputri, Jelita Febrilia Bryan Wangidjaja Budiman, Johan Arief Cindy Fransisca Cindy Fransisca, Cindy Ciptadhi Tri Oka, Ciptadhi Tri Elline Ferry Sandra Gabriella Nasseri Hanny Japarto Howuk, Barnabas Juliani Hardja B, Magdalena Keshia Kristin Luthfiyah, Syifa Meiny Faudah Amin Melanie S Djamil Melisa Gunawan Okkinardo Arief Olivia Nauli Komala, Olivia Patricia Revana Renata Maharani Salsabila, Sasya Anursyah Sindy Fidelia Stephanie Aipassa Syarafina, Amiretta Taufiq Ariwibowo Thalib, Sofia Trisfilha, Pretty Trisfilha, Trisfilha Tyatana, Risha Nadira Vica Kartikasari Widyastuti, Wiena