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Perbedaan kadar interleukin-6 dalam darah vena antara pasien dengan dan tanpa periodontitis apikalisThe difference of interleukin-6 level in venous blood between patients with and without existing apical periodontitis Taufiq Ariwibowo; Meiny Faudah Amin; Ratih Nur Baiti
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 33, No 3 (2021): Desember 2021
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jkg.v33i3.34020

Abstract

Pendahuluan: Periodontitis apikalis adalah peradangan dan kerusakan jaringan pada daerah apikal jaringan periodonsium. Terjadi akibat pulpa nekrosis mengalami perluasan infeksi dari bakteri saluran akar menuju apeks gigi. Penelitian terdahulu membuktikan bahwa periodontitis apikalis dapat dikaitkan dengan peningkatan kadar interleukin-6. Interleukin-6 adalah salah satu sitokin pro-inflamatori yang mempunyai peran penting dalam respon inflamasi. Tujuan penelitian menganalisis perbedaan kadar interleukin-6 dalam darah vena antara pasien dengan periodontitis apikalis dan pasien tanpa periodontitis apikalis. Metode: Jenis penelitian observasional analitik dengan dua puluh sampel stok darah vena yang terdiri atas 10 sampel darah vena pasien dengan periodontitis apikalis dan 10 sampel darah vena pasien tanpa periodontitis apikalis. Serum darah yang telah dipisahkan dengan metode sentrifugasi dipakai sebagai sampel uji enzyme-linked immunosorbent assay (ELISA) sandwich untuk mengukur kadar IL-6 dengan membaca nilai absorbansi dan kurva standar. Data yang diperoleh dianalisis menggunakan uji Shapiro-Wilk, dilanjutkan dengan uji T tidak berpasangan. Hasil: Kadar IL-6 teridentifikasi pada semua sampel. Sampel darah vena pasien tanpa periodontitis apikalis memiliki kadar IL-6 berkisar antara 4,7-18,74 mg/L, sedangkan kadar IL-6 pada pasien dengan periodontitis apikalis 4,0-90,75 mg/L. Uji T tidak berpasangan menunjukan bahwa terdapat perbedaan yang bermakna (p=0.02). Simpulan:  Kadar IL-6 pada darah vena dengan periodontitis apikalis lebih tinggi dibandingkan kadar IL-6 pada tanpa periodontitis apikalis.Kata kunci: periodontitis apikalis; interleukin-6; Enzim-Linked Immunosorbent Assay SandwichABSTRACTIntroduction: Apical periodontitis is inflammation and tissue damage in the apical area of the periodontium. Occurs due to pulp necrosis experiencing an expansion of infection from root canal bacteria to the apex of the tooth. Previous studies have shown that apical periodontitis can be associated with elevated levels of interleukin-6. Interleukin-6 is a pro-inflammatory cytokine that has a vital role in the inflammatory response. The study aimed to analyze differences in interleukin-6 levels in venous blood between patients with apical periodontitis and patients without apical periodontitis. Methods: This research was an analytic observational study with twenty venous blood samples consisting of 10 venous blood samples from patients with apical periodontitis and ten venous blood samples from patients without apical periodontitis. Blood serum that has been separated by centrifugation method was used as a sandwich enzyme-linked immunosorbent assay (ELISA) test sample to measure IL-6 levels by reading absorbance values and standard curves. The data obtained were analyzed using the Shapiro-Wilk test, followed by an unpaired T-test. Results: IL-6 levels were identified in all samples. Venous blood samples from patients without apical periodontitis had IL-6 levels ranging from 4.7-18.74 mg/L, while IL-6 levels in patients with apical periodontitis were 4.0-90.75 mg/L. The unpaired t-test showed that there was a significant difference (p=0.02). Conclusion: IL-6 levels in venous blood with apical periodontitis were higher than IL-6 levels in those without apical periodontitis. Keywords: apical periodontitis; interleukin-6; Enzyme-Linked Immunosorbent Assay Sandwich 
PEMELIHARAAN KESEHATAN GIGI DAN MULUT DALAM RANGKA PENCEGAHAN KELAINAN MALOKLUSI DI MASA PANDEMI COVID-19 Johan Arief Budiman; Janti Sudiono; Meiny Faudah Amin; Sariany Pancasari Audry Ariefin; Siti Haerunnisah; Syarifa Saraswati; Zafira Rasya; Arda Arda
Jurnal AKAL : Abdimas dan Kearifan Lokal Vol. 3 No. 1 (2022): Jurnal AKAL : Abdimas dan Kearifan Lokal
Publisher : Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2601.226 KB) | DOI: 10.25105/akal.v3i1.9754

Abstract

Prevalensi maloklusi di Indonesia masih sangat tinggi yaitu sekitar 80% dari jumlah penduduk dan merupakan salah satu masalah kesehatan gigi dan mulut yang cukup besar. Maloklusi sering ditemukan pada anak-anak masa periode gigi campur, yaitu saat gigi sulung dan gigi permanen secara besamaan berada di dalam rongga mulut yang di mulai dari sekitar usia 6 tahun hingga usia 12 tahun. Deteksi kelainan maloklusi dapat dimulai pada usia 7 tahun. Tujuan kegiatan ini adalah membiasakan anak-anak untuk menyikat gigi sedini mungkin dan memberikan pengetahuan tentang cara sikat gigi yang baik dan benar dalam rangka pencegahan maloklusi. Pelaksanaan program merawat gigi pada anak serta mengenali kelainan maloklusi meliputi tahapan proses ceramah, dan tanya jawab melalu media Webinar Zoom. Materi meliputi alasan pembatasan kunjungan ke dokter gigi di masa pandemi, penyebab terbanyak kunjungan ke dokter gigi (sakit karena gigi berlubang), proses terjadinya, cara pencegahannya, dan tips deteksi dini dalam rangka pencegahan maloklusi. Dengan 34 peserta, keberhasilan kegiatan ini dilihat dari peningkatan pengetahuan. Skor hasil dari pretest dan posttest selanjutnya akan dibandingkan dan diketahui apakah ada perubahan pengetahuan sebelum dan sesudah pelatihan. Hasil kegiatan yang dilakukan melalui media webinar menunjukkan hasil yang memuaskan. Terdapat peningkatan nilai posttest para peserta penyuluhan sebesar 10,5%.
Perbedaan Jumlah Porphyromonas endodontalis pada Diagnosis Pulpitis Ireversibel dan Nekrosis Pulpa (Penelitian) Taufiq Ariwibowo; Bryan Wangidjaja; Meiny Faudah Amin
Jurnal Kedokteran Gigi Terpadu Vol. 1 No. 2 (2019): Jurnal Kedokteran Gigi Terpadu
Publisher : Fakultas Kedokteran Gigi Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (412.393 KB) | DOI: 10.25105/jkgt.v1i2.6394

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Background: Pulp diseases can be affected by bacterial invasion such as Porphyromonas endodontalis. This bacterial invasion can lead to pulp diseases which include irreversible pulpitis and necrotic pulp. Purpose: To determine Porphyromonas endodontalis difference between teeth with irreversible pulpitis and necrotic pulp. Method: Samples were taken from root canals fluid of 18 teeth, 9 teeth with irreversible pulpitis, 9 teeth with necrotic pulp, using paper point. The paper point was placed in sterile Eppendorf tube, then DNA were extracted using Heat-Shock DNA Extraction method. Real-time PCR was used to collect the data. The conversion of microbiological data were used standard curve of Porphyromonas endodontalis. Result: The average of Real-time PCR on irreversible pulpitis was 6.9 x 10-12 ng/mL and on necrotic pulp was 1.3 x 10-12 ng/mL. However, t independent test showed there was no significant difference of Porphyromonas endodontalis between teeth with irreversible pulpitis and necrotic pulp (p>0,05) Conclusion: There was no difference in the total number of Porphyromonas endodontalis between teeth with irreversible pulpitis and necrotic pulp.
Efek Ekstrak Daun Pluchea indica terhadap Hambatan Pertumbuhan Porphyromonas gingivalis Taufiq Ariwibowo; Meiny Faudah Amin; Putri Nur Pratiwi
Jurnal Kedokteran Gigi Terpadu Vol. 3 No. 1 (2021): Jurnal Kedokteran Gigi Terpadu
Publisher : Fakultas Kedokteran Gigi Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (565.099 KB) | DOI: 10.25105/jkgt.v3i1.9516

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Background: Porphyromonas gingivalis is one of the bacteria causes primary root canal infections. Bacterial elimination using root canal irrigation agents is important in successful root canal treatment. NaOCl as the gold standard is known to have disadvantages such as irritating periapical tissue. Active compounds in Pluchea indicaleaves such as flavonoids, tannins and terpenoids posses antibacterial effects. Objectives: To determine the effect of Pluchea indica leaves extract on P. gingivalis growth inhibition. Method: This study is an in vitro experimental laboratory of  agar well difusion method used Pluchea indica leaves extract in several concentrations (6,25%, 12,5%, 25%, 50%, and 100%) and NaOCl 5,25% as a positive control. The sample in this study was P. gingivalis. Result: This study showed that the diameter of inhibitor zone against P. gingivalis was exists on concentration of 50% and 100%, their values are 10,216 mm and 13,023 mm. The positive control showed an average inhibition zone of 12.803mm. Conclusion: Pluchea indica leaves extract showed antibacterial effect against P.gingivalis at the concentration of 50% and 100%. The 100% concentration had the largest inhibition zone and was equivalent to positive control in inhibiting P.gingivalis.
Efek Antibakteri Tumbuhan Pepaya (Carica Papaya L.) Terhadap Porpyromonas Gingivalis (Laporan Penelitian) Meiny Faudah Amin; Taufiq Ariwibowo; Febria Febria
Jurnal Kedokteran Gigi Terpadu Vol. 3 No. 1 (2021): Jurnal Kedokteran Gigi Terpadu
Publisher : Fakultas Kedokteran Gigi Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (359.652 KB) | DOI: 10.25105/jkgt.v3i1.9922

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Background: Porphyromonas gingivalis is one of the bacteria that cause pulp necrosis, NaOCl is an irrigation solution that has become the gold standard in endodontic treatment, however NaOCl is toxic, so other alternative materials are needed. One usable material is papaya plant (Carica papaya L.) which has antibacterial properties. Objective: to know the differences in the activity of papaya leaves extract, papaya seed extract, and papaya fruit juice in inhibiting P.gingivalis. Methods: This study is an in vitro experimental laboratory of agar well diffusion method used papaya seeds extract, papaya leaves extract and papaya fruit juice in several consentrations (6.25%,12.5%,25%,50%, and 100%) and NaOCl 5,25% as positive control. Results: This study showed that the diameter of inhibitory zone against P.gingivalis was found on 2 concentrations of papaya seed extract, a concentration of 100% with an average 23,43 mm and concentration of 50% with an avarege 16,43 mm. The inhibition zone test of papaya leaves extract and papaya fruit juice against P.gingivalis was not found around the well. Conclusion: Papaya seed extract was able to inhibit P.gingivalis in certain concentration, while papaya leave extract and papaya fruit juice were not able to inhibit the growth of P.gingivalis.
CARA MENGURANGI BAKTERI JAHAT DI RONGGA MULUT DENGAN METODE "SIBENUR" PADA KOMUNITAS SMP ISLAM AL AZHAR 08 BEKASI. Ciptadhi Tri Oka Binartha; Meiny Faudah Amin; Sri Ratna Laksmiastuti; Yessy Ariesanti
JURNAL ABDIMAS KESEHATAN TERPADU Vol. 1 No. 2 (2022): Jurnal Abdimas Kesehatan Terpadu
Publisher : Fakultas Kedokteran Gigi, Universitas Trisakti bekerjasama dengan Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (784.912 KB) | DOI: 10.25105/jakt.v1i2.15473

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According to Riskesdas data in 2018, the prevalence of caries in Indonesia is 66% and data on toothbrush behavior in the population aged 3 years and over shows that the correct brushing behavior is still below 4%. The covid-19 pandemic situation that is still unfinished has delayed the maintenance of dental and oral health, especially routine examinations of dental and oral health The school age period is the most important time to start maintaining healthy teeth and mouth, this is because the good quality of health is a reflection of the quality of human resources. In an effort to help deal with this problem, counseling and education were carried out on how to maintain oral and dental health in the Al Azhar 8 Islamic junior high school (SMPI) community, Bekasi. The purpose of this activity is to increase knowledge and provide training on how to maintain and care for dental and oral health while at home. The method used in this counseling and training is carried out online using the zoom application. The main material given to the participants was to introduce how to maintain oral and dental health with the "SIBENUR" method (dental floss brush and gargle). This method can make it easier for counseling participants to remind them how to maintain healthy teeth and mouth at home. Evaluation of knowledge improvement in this counseling uses pretest and post test using the Google Form. The aims of this test is to measure the level of knowledge. The results obtained from 3 groups of respondents divided by class group showed data on an average increase in knowledge of 76%. It is hoped that this increase in knowledge can also improve the degree of dental and oral health in the community, especially in the target partner community
Perbedaan kadar tumor necrosis factor-alpha dalam darah vena pada pasien dengan periodontitis apikalis dan pulpa normalThe different of tumor necrosis factor-alpha level in venous blood in patient with apical periodontitis and normal pulp Meiny Faudah Amin; Taufiq Ariwibowo; Mutiara Risa Anggriani
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 34, No 3 (2022): Desember 2022
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jkg.v34i3.37885

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ABSTRAKPendahuluan: Periodontitis Apikalis (PA) adalah peradangan jaringan periapikal yang berawal dari lesi karies. Tubuli dentin yang terbuka akibat adanya karies menjadi jalan masuk bakteri dan produknya. Proses ini akan terus berlanjut hingga mengakibatkan nekrosis pulpa dan peradangan berat sampai ke periapikal gigi. Infeksi pada gigi ini akan memediasi peradangan dan menginduksi sel untuk mengeluarkan sitokin proinflamasi. Tumor Necrosis Factor-Alpha (TNF-α) merupakan salah satu sitokin pada respon inflamasi akut bakteri Gram negatif dan protein pada fase akut. Tujuan penelitian menganalisis perbedaan jumlah kadar TNF-α di dalam sirkulasi darah vena antara pasien dengan periodontitis apikalis dan pasien dengan pulpa normal. Metode: Penelitian ini merupakan observasional analitik dengan rancangan cross sectional study. Sampel diambil dari darah vena 10 orang pasien dengan periodontitis apikalis dan 10 pasien normal (tanpa adanya gigi yang terinfeksi PA). Sampel ditentukan berdasarkan hasil anamnesis, pemeriksaan klinis yang dilakukan seperti tes perkusi atau palpasi dan terdapatnya gambaran radiolusen di apikal gigi. Kadar TNF-α, diperiksa menggunakan metode Sandwich ELISA, kemudian data dianalisis menggunakan uji statistik T tidak berpasangan. Hasil: Kadar TNF-α pada sampel darah vena pasien dengan periodontitis apikalis berkisar antara 0,068-0,121 mg/L, sedangkan kadar TNF-α pada pasien dengan pulpa normal berkisar 0,061- 0,143 mg/L. Uji T-test independent menunjukan nilai p=0,389 (p<0,05). Simpulan: Kadar TNF-α pada pasien dengan PA dan pulpa normal tidak memiliki perbedaan yang signifikan secara statistik.Kata kunci: periodontitis apikalis; porphyromonas endodontalis; tumor necrosis factor alpha (TNF-α); pulpa normalABSTRACTIntroduction: Apical periodontitis (AP) is an inflammation of periapical tissue initiated by caries lesions. The exposed dentin tubules due to caries are an entrance for bacteria and its products. This process will continue to result in pulp necrosis and severe inflammation extending to the periapical area of the tooth. The infection of this tooth will mediate inflammation and induce cells to release proinflammatory cytokines. Tumor Necrosis Factor-Alpha (TNF-α) is one of the acute inflammatory response cytokines in Gram-negative bacteria and protein in the acute phase. The purpose of this study is to analyze different levels of TNF-α in venous blood circulation between patients with AP and normal pulp. Methods: The type of study was an observational analytic with cross sectional study design. The blood samples were collected from 10 patients with AP and 10 with normal pulp. Samples were determined from medical history, percussion and palpation clinical examination, and apical radiolucency. TNF-α levels were then examined using the Sandwich ELISA method, and data were analyzed using an unpaired T-test. Results: TNF-α levels in venous blood samples from patients with AP ranged from 0,068-0,121 mg/L, and TNF-α levels in patients with normal pulp were  0,061- 0,143 mg/L. Unpaired t-test showed no significant difference p=0,389 (p<0.02). Conclusion: TNF-α levels in patients with AP and normal pulp did not differ statistically.Keywords: apical periodontitis; porphyromonas endodontalis; tumor necrosis factor alpha (TNF-α); normal pulp
Penilaian Hasil Penyuluhan Kesehatan Gigi dan Mulut Pada Komunitas Alumni Abang None Jakarta Selatan Taufiq Ariwibowo; Aditya Pratama Sarwono; Sri Ratna Laksmiastuti; Didi Nugroho; Meiny Faudah Amin
JURNAL ABDIMAS KESEHATAN TERPADU Vol. 2 No. 1 (2023): Jurnal Abdimas Kesehatan Terpadu
Publisher : Fakultas Kedokteran Gigi, Universitas Trisakti bekerjasama dengan Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/jakt.v2i1.16786

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It is important for everyone, regardless of age, to take into account their dental and oral health to enhance their overall well-being. Dentists have the responsibility of educating the public on proper dental and oral care in order to attain the best possible dental and oral health. Nevertheless, there are still numerous individuals who do not prioritize their dental and oral health. With the aim of fulfilling the Tridharma mission in Community Service, the Faculty of Dentistry at Trisakti University has implemented Public Health Services tha provide counseling on oral and dental health for families, staring with the elderly, general dental health, childrens dental health, and safe medications for families. Through the use of a questionnaire, an increase in knowledge was observed among individuals of different age groups regarding oral health care.
Pengaruh ekstrak daun jambu biji (psidium guajava linn.) Terhadap biofilm enterococcus faecalis Alyssa Devina Amalia; Taufiq Ariwibowo; Meiny Faudah Amin
Jurnal Kedokteran Gigi Terpadu Vol. 5 No. 1 (2023): Jurnal Kedokteran Gigi Terpadu
Publisher : Fakultas Kedokteran Gigi Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/jkgt.v5i1.17172

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Background: Enterococcus faecalis biofilm is one of the causes of root canal treatment failure. The success of root canal treatment is achieved by mechanical and chemical functions. Irrigation is the only way to reach areas of the root canal wall that instrumentation cannot reach. Guava (Psidium guajava Linn.) leaves contain compounds such as flavonoids, saponins, tannins, triterpenoids and essential oils which have antibacterial properties. Objective: To determine the anti-biofilm effect of guava leaf extract (Psidium guajava Linn.) on Enterococcus faecalis Methods: Microdilution method laboratory experimental study using Enterococcus faecalis ATCC 29212 culture with P. guajava leaf extract assay concentrations of 100%, 80%, 60%, 40% and 20%. Results: In testing P. guajava leaf extract against E. faecalis biofilm, it was found that 60% concentration had the highest inhibition. Conclusion: Guava leaf extract (Psidium guajava Linn.) effectively inhibits Enterococcus faecalis biofilm growth.
Edukasi Pertolongan Pertama Kasus Gawat Darurat Gigi pada Remaja Wilayah Kayu Mas Jakarta Timur Faudah Amin, Meiny; Yusra, Yohana; Juliawati, Mita; Kusnoto, Joko; Ie Elline
JURNAL ABDIMAS KESEHATAN TERPADU Vol. 2 No. 2 (2023): Jurnal Abdimas Kesehatan Terpadu
Publisher : Fakultas Kedokteran Gigi, Universitas Trisakti bekerjasama dengan Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/jakt.v2i2.18737

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Emergency dental issues such as throbbing toothaches, dental trauma (fractures, dislodgment from the gums), teeth pierced by orthodontic wires, and wires from dental braces causing injuries by piercing the gums, are some of the health problems that can result in prolonged suffering for those experiencing them. Every case has a different level of emergency. Although for some people it is not considered a real emergency, it is still a condition that urgently requires proper action before going to the dentist. Therefore, there is a need for increased knowledge to provide first aid before visiting a dentist. Collaborative efforts between medical professionals, alumni, and professional students from the Faculty of Dentistry at Trisakti University conducted an activity involving education for the adolescent community at the Integrated Child-Friendly Public Space (RPTRA) in Kayu Mas, East Jakarta, on March 16, 2023. Before and after the distribution of education, pre and post-tests were conducted, showing significant improvements in knowledge regarding dental emergencies. The conclusion drawn from this activity underscores the importance of knowing the initial steps to handle dental emergencies in order to receive appropriate medical care.