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All Journal Indonesian Journal of Biotechnology Pharmascience The Indonesian Biomedical Journal Sinnun Maxillofacial Journal Jurnal AKAL: Abdimas dan Kearifan Lokal Jurnal Abdimas Kesehatan Terpadu
Trisfilha, Pretty
Department Of Oral Biology Sub‐division Of Oral Pathology, Faculty Of Dentistry, Universitas Trisakti, Jakarta 11440, Indonesia
Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Dentistry Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Transportation

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Cytotoxicity of Alpinia galanga Rhizome Crude Extract on NIH-3T3 Cells Ferry Sandra; Janti Sudiono; Pretty Trisfilha; Deviyanti Pratiwi
The Indonesian Biomedical Journal Vol 9, No 1 (2017)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v9i1.212

Abstract

BACKGROUND: Alpinia galanga (A. galanga) was reported as a potential medicinal source due to its wide effect. A. galanga rhizome crude extract (ARCE) was reported to have high cytotoxic effect in cancer cells, but low in normal cells. However half maximal inhibitory concentration (IC50) of ARCE is not clearly known yet. Hence, current study was conducted to investigate the IC50 of ARCE in normal standard fibroblast cell line, NIH-3T3 cells.METHODS: Rhizomes of A. galanga were collected, peeled, dried, milled and weighed. Extraction was performed using maceration method, then filtered and evaporated. ARCE with various concentrations were applied in NIH-3T3 cells for 24 or 48 hours. Cells were documented and counted with 3-(4,5-dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium bromide (MTT) assay.RESULTS: Five hundreds grams of simplicia were macerated with ethanol and evaporated, 1 mg/mL crude extract with total volume of 114 mL was obtained. By addition of ARCE in NIH-3T3 cell culture, number of NIH-3T3 cells were shown less when treated with higher concentration of ARCE. Cell numbers of 0, 3.125, 6.25, 12.5, 25 and 50% ARCE treatment for 24 hours are 11,531, 11,352, 10,920, 10,365, 9,471, 8,360, respectively, meanwhile for 48 hours are 13,219, 12,686, 12,278, 11,390, 10,279, 8,390, respectively.CONCLUSION: IC50 of ARCE in 24 hours treatment was 620.5 mg/mL, while in 48 hours treatment was 666.6 mg/mL. Hence, ARCE is suggested to have low cytotoxic effect in NIH-3T3 cells.KEYWORDS: Alpinia galanga, ginger, extract, cytotoxic, MTT, NIH-3T3 
Penurunan Produksi Reactive Oxygen Species (ROS) Fibroblas dengan Nano Kitosan Kumbang Tanduk (Xylotrupes gideon) Komariah Komariah; Cynthia Priscilla; Rahman Wahyudi; Pretty Trisfilha; Didi Nugroho
Jurnal Pharmascience Vol 10, No 1 (2023): Jurnal Pharmascience
Publisher : Program Studi Farmasi FMIPA Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jps.v10i1.15006

Abstract

Fibroblas berperan dalam proses penyembuhan luka dengan pembentukan pembuluh darah, penggerakan serta proliferasi, deposit matriks ekstraseluler dan remodeling jaringan. Pada penyembuhan luka, netrofil dan makrofag akan mengalami peningkatan penggunaan oksigen sehingga memproduksi ROS, peningkatan produk ini akan menyebabkan stres oksidatif pada fibroblas yang akan mempengaruhi proses migrasi dan proliferasi selama proses penyembuhan luka. Kandungan gugus amino (-NH2) dan gugus hidroksil (-OH) pada nano kitosan mampu mengurangi stres oksidatif  fibroblas. Penelitian bertujuan untuk mengetahui kemampuan nano kitosan X. gideon dalam menurunkan produksi ROS fibroblas. Penelitian terbagi menjadi enam kelompok terdiri dari kontrol negatif (H2O2), kontrol pembanding, kontrol asam askorbat, nano kitosan konsentrasi 200, 400, dan 600 µg/mL. Produksi ROS dilihat menggunakan probe 2′,7′-Dichlorodihydrofluorescein diacetate (H2DCF-DA). Produksi fibroblas diperlihatkan dengan intensitas fibroblas terflouresen hijau dihitung menggunakan software image J. Rerata produksi ROS pada kelompok nano kitosan 200, 400, dan 600 µg/mL berbeda signifikan dengan kontrol negatif (p<0,05), sedangkan yang diberikan asam askorbat tidak  signifikan (p>0,05) dengan perlakuan 200 dan 600 µg/mL, namun signifikan dengan nano kitosan 400 µg/mL. Nano kitosan X. gideon mampu menurunkan produksi ROS fibroblas pada konsentrasi 400 µg/mL. Kata Kunci: Nano Kitosan, X. gideon, ROS, Fibroblas, Fluoresen  Fibroblasts play a role in wound healing by forming blood vessels, mobilizing and proliferation, depositing extracellular matrix and tissue remodelling. In wound healing, neutrophils and macrophages will experience an increase in oxygen use, resulting in ROS production. An increase in these products will cause oxidative stress in fibroblasts, affecting the migration and proliferation processes during the wound healing process. The content of amino groups (-NH2) and hydroxyl groups (-OH) in nano chitosan can reduce fibroblast oxidative stress. This study aimed to determine the ability of X. gideon nano chitosan to reduce fibroblast ROS production. The study was divided into six groups: negative control (H2O2), control control, ascorbic acid control, and nano chitosan concentrations of 200, 400 and 600 µg/mL. ROS production observes using the 2′,7′-Dichlorodihydrofluorescein diacetate (H2DCF-DA) probe. The intensity of greenfluorescent fibroblasts calculated using Image J software showed the production of fibroblasts. The mean ROS production in the 200, 400, and 600 µg/mL nano-chitosan groups was significantly different from the negative control (p<0.05), whereas those given ascorbic acid were insignificant. (p>0.05) with 200 and 600 µg/mL treatments, but significant with 400 µg/mL nano chitosan. X. gideon nano chitosan reduced fibroblast ROS production at 400 µg/mL concentration.
Chitosan Xylotrupes gideon encapsulated lemongrass leaf ethanol extract reduce H2O2‐induced oxidative stress in human dermal fibroblast Komariah Komariah; Pretty Trisfilha; Rahman Wahyudi; Nada Erica; Didi Nugroho; Yessy Ariesanti; Sarat Kumar Swain
Indonesian Journal of Biotechnology Vol 28, No 4 (2023)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.81544

Abstract

During phagocytosis, phagocyte cells discharge reactive oxygen species referred to as respiratory bursts, inducing a rise in pro‐oxidants and subjecting the cell to oxidative stress. Such stress is a biological mechanism related to an imbalance in pro‐oxidant/antioxidant homeostasis, which generates toxic reactive oxygen. Encapsulation is a coating process to improve the stability of bioactive compounds from lemongrass extract. Therefore, this study aims to determine the encapsulation activity of lemongrass leaf extract with chitosan X. gideon (LEChXg) to reduce the oxidative stress of fibroblasts. The research used the human dermal fibroblast (HDF) cell line, comprising negative and positive controls and use of LEChXg 100, 200, 300, 400, and 500 µg/mL. HDF cell migration was evaluated by employing the scratch wound healing method and the wound closure was oberseved at 0, 2, 4, 6, and 24 h intervals. The cell proliferation was observed at 24, 48, and 72 h using CCK‐8 at a 450 nm wavelength. The results showed that the observations at 0, 2, and 4 h did not demonstrate any significant difference on the cell migration (p > 0.05) among the groups. However, the wound closure at 4 and 6 h showed a significant difference (p < 0.05) with LEChXg 300 µg/mL. Despite the lack of any significant variation observed up to 24 h, fibroblast subjected to the stressor did not achieve complete closure. The groups treated with LEChXg were more stable in maintaining fibroblast proliferation up to the end of the observation than those with stressors at 24, 48, and 72 h. Fibroblast induced with a stressor was also more stable in maintaining migration and proliferation in groups receiving LEChXg 300 µg/mL.
Pemberdayaan Anak dan Pengurus Panti Yatim Mizan Amanah Bintaro dalam Menjaga Kesehatan Rongga Mulut Dwisaptarini, Ade Prijanti; Trisfilha, Pretty; Juliani, Magdalena; Ariwibowo, Taufiq; Hanin, Isya
JURNAL ABDIMAS KESEHATAN TERPADU Vol. 3 No. 2 (2024): Jurnal Abdimas Kesehatan Terpadu
Publisher : Fakultas Kedokteran Gigi, Universitas Trisakti bekerjasama dengan Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/jakt.v3i2.21009

Abstract

The Covid-19 pandemic has caused more and more children to lose their parents, thus requiring attention from various interested parties to deal with this problem. This oral health counseling effort needs to be carried out so that the orphanage administrators and the children under them can increase knowledge, awareness, and then are expected to form a behavior that will have a positive impact on the oral health of these children. PkM activities that will be carried out include training, counseling and education on how to maintain oral health in orphanages, including the causes of caries, how to detect early dental caries, how to prevent caries and actions taken if dental caries has occurred. Toothbrush and tongue brush training will also be conducted. It is hoped that this PkM can be a provision for improving oral health in the extended family of the orphanage. Outputs in the form of educational videos, posters and teaching aids will be submitted for Intellectual Property Rights (HKI) and are expected to be used by the orphanage.  
PENYULUHAN DAN PELATIHAN MENJAGA KESEHATAN GIGI DAN MULUT DI SDN WIJAYA KUSUMA 02 PAGI JAKARTA BARAT Trisfilha, Pretty; Trioka Binartha, Ciptadhi; Tjandrawinata, Rosalina; Priandini, Dewi; Handojo, James
Jurnal AKAL: Abdimas dan Kearifan Lokal Vol. 6 No. 1 (2025): Jurnal AKAL : Abdimas dan Kearifan Lokal
Publisher : Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/akal.v6i1.20985

Abstract

Gigi berlubang atau yang dikenal dengan karies masih menjadi salah satu masalah utama dalam bidang kesehatan gigi dan mulut pada anak usia sekolah. Hal ini juga dijumpai pada siswa SDN Wijaya Kusuma 02 Pagi Jakarta Barat. Masalah ini dapat  disebabkan oleh kurangnya edukasi dan informasi mengenai cara merawat kesehatan gigi dan mulut dengan baik dan benar. Oleh karena itu perlu diadakan kegiatan edukasi kesehatan gigi-mulut kepada guru dan siswa SDN Wijaya Kusuma 02 Pagi, yang bertujuan untuk meningkatkan  pengetahuan  guru dan siswa SDN Wijaya Kusuma 02 Pagi mengenai cara merawat Kesehatan gigi dan mulut dengan baik dan benar. Metode yang digunakan dalam penyuluhan ini adalah melalui pemberian cerita dengan menggunakan media audiovisual, simulasi dan praktek menyikat gigi yang benar, serta pembagian perlengkapan menyikat gigi dan media KIE menyikat gigi. Melalui metode tersebut diharapkan dapat menjadi faktor predisposisi bagi siswa/i SDN Wijaya Kusuma 02 Pagi untuk mempraktekan perilaku menyikat gigi dengan benar dan teratur. Berdasarkan hasil pre dan post test diketahui terdapat peningkatan pengetahuan siswa SDN Wijaya Kusuma 02 Pagi mengenai cara merawat kesehatan gigi dan mulut pasca penyuluhan. Sehingga dapat disimpulkan bahwa penyuluhan kesehatan gigi dan mulut dengan metode cerita dengan menggunakan media audiovisual dan simulasi mampu meningkatkan pengetahuan siswa mengenai Kesehatan gigi dan mulut.
Vakuolisasi Sitoplasma Sel Epitel Mukosa Mulut sebagai Parameter Perubahan Seluler pada Perokok Luthfiyah, Syifa; Trisfilha, Pretty; Sudiono, Janti
Sinnun Maxillofacial Journal Vol. 7 No. 02 (2025): Oktober 2025
Publisher : Fakultas Kedokteran Gigi Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/smj.v7i02.191

Abstract

Pendahuluan: Merokok merupakan faktor risiko utama berbagai penyakit dan dapat menyebabkan kematian akibat kandungan zat karsinogenik yang dapat merusak DNA serta menimbulkan efek sitotoksik. Salah satu parameter yang dapat digunakan untuk mendeteksi kerusakan DNA adalah vakuolisasi sitoplasma. Tujuan Penelitian: Menganalisis perbedaan jumlah sel epitel mukosa mulut yang mengalami vakuolisasi sitoplasma antara perokok dan non-perokok, serta membandingkan pengaruh jenis rokok yang digunakan. Bahan dan Metode: Penelitian observasional analitik dengan desain cross-sectional ini melibatkan 60 responden, terdiri atas 30 perokok dan 30 non-perokok. Pemeriksaan sel dilakukan menggunakan sitologi eksfoliatif dengan pewarnaan Papanicolaou. Data dianalisis secara statistik dengan tingkat signifikansi 0,05. Hasil: Terdapat perbedaan bermakna jumlah sel dengan vakuolisasi sitoplasma antara kelompok perokok dan non-perokok (p < 0,05). Namun, tidak ditemukan perbedaan bermakna dalam jumlah sel dengan vakuolisasi sitoplasma antara perokok rokok putih, rokok kretek, rokok elektrik, maupun kombinasi rokok konvensional dan elektrik (p > 0,05). Kesimpulan: Merokok berhubungan dengan peningkatan vakuolisasi sitoplasma pada sel epitel mukosa mulut, yang menandakan adanya kerusakan sel akibat paparan asap rokok. Semua jenis rokok yang digunakan memiliki potensi serupa dalam menimbulkan efek sitotoksisitas pada mukosa mulut.
Co-Authors Ariesanti, Yessy Cynthia Priscilla Deviyanti Pratiwi Dewi Priandini, Dewi Didi Nugroho Didi Nugroho Dwisaptarini, Ade Prijanti Ferry Sandra Isya Hanin James Handojo Janti Sudiono Janti Sudiono Komariah Komariah Komariah Komariah Luthfiyah, Syifa Magdalena Juliani Nada Erica Rahman wahyudi Rahman Wahyudi Rosalina Tjandrawinata Sarat Kumar Swain Taufiq Ariwibowo Trioka Binartha, Ciptadhi