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All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Riset Akuakultur
Ketut Sugama
Research Centre for Aquaculture
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Veterinary

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POLA PERTUMBUHAN DAN VARIASI GENETIK BERBASIS DNA MIKROSATELIT DARI TIGA POPULASI IKAN BARAMUNDI Lates calcarifer Khotimah, Fitriyah Husnul; Alimuddin, Alimuddin; Soelistyowati, Dinar Tri; Nuryati, Sri; Arfah, Harton; Sugama, Ketut; Permana, Gusti Ngurah; Sembiring, Sari Budi Moria; Haryanti, Haryanti
Jurnal Riset Akuakultur Vol 18, No 1 (2023): (Maret 2023)
Publisher : Politeknik Kelautan dan Perikanan Jembrana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jra.18.1.2023.49-59

Abstract

Benih ikan baramundi Lates calcarifer diperoleh dari pemijahan alami dengan jumlah induk terbatas sehingga variabilitas pertumbuhan dan kelangsungan hidup antar-batch menjadi tinggi. Penelitian dilakukan untuk menganalisis pertumbuhan dan mengevaluasi variasi genetik ikan kakap putih populasi Australia, Situbondo dan Lampung hasil domestikasi dan dibudidaya di hatcheri skala rumah tangga (HSRT). Sebanyak 10 ekor ikan barramundi dari setiap populasi digunakan untuk analisis variabilitas genetik dengan dua lokus mikrosatelit, yaitu Lca21 dan Lca32. Selanjutnya, data mikrosatelit diolah menggunakan software genetic analysis in excel (GenAlEx 6.51b2). Hasil yang diperoleh menunjukkan bahwa pertumbuhan panjang dan bobot tubuh serta laju pertumbuhan spesifik ikan baramundi dari Australia lebih tinggi dibandingkan dari Situbondo dan Lampung (P<0,05); sedangkan ikan baramundi dari Situbondo dengan Lampung adalah sama (P>0,05). Jumlah alel setiap lokus ikan baramundi berkisar 2-8 alel dan heterozigositas tertinggi dimiliki oleh ikan barramundi asal Situbondo (0,85), diikuti Lampung (0,65) dan paling rendah dari Australia (0,54). Dari hasil riset tersebut dapat disimpulkan bahwa ketiga populasi ikan baramundi hasil domestikasi dan dipeliharan dalam sistem HSRT memenuhi kelayakan untuk digunakan untuk kegiatan hibridisasi atau membentuk populasi awal (sintetis). Barramundi seeds, Lates calcarifer are usually sourced from natural spawning using a limited number of broodstock. Therefore, the growth and survival rate of these seeds vary greatly between batches. The research was performed to determine the growth pattern and genetic variations of barramundi seed populations produced from domesticated broodstock sourced from Australia, Situbondo, and Lampung and reared in small-scale hatcheries. Ten individuals of barramundi from each population were used for microsatellite analysis using two microsatellite loci, namely: Lca 21 and Lca 32. The resulted microsatellite data was processed using the genetic analysis available in Excel software (GenAlEx 6.51b2). The results showed that the growth in length and body weight as well as the specific growth rate of barramundi seeds produced from Australia broodstock were higher than that of Situbondo and Lampung (P<0.05) while the later two were similar (P>0.05). The number of microsatellite alleles ranged from 2-8 and the highest heterozygosity was obtained by barramundi seeds produced by Situbondo (0.85), followed by Lampung (0.65), dan Australia (0.54) broodstock. From the results of the research, it can be concluded that the three populations of barramundi fish, which were domesticated and reared in the HSRT system, meet the criteria for use in hybridization program or for forming a synthetic population.
Effects of Humic Acid Addition to Feeds With Heavy Metal Cadmium Contamination From Green Mussels on the Growth Performance of Asian Seabass Rasidi, Rasidi; Jusadi, Dedi; Setiawati, Mia; Yuhana, Munti; Jr., Muhammad Zairin; Sugama, Ketut
BIOTROPIA Vol. 26 No. 3 (2019): BIOTROPIA Vol. 26 No. 3 December 2019
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (236.409 KB) | DOI: 10.11598/btb.2019.26.3.1114

Abstract

Although it contains heavy metals, the highly nutritious green mussel Perna viridis is used as a fish meal replacement in fish diets. Fortunately, humic acid (HA) has the ability to chelate heavy metals in animal feeds. Its addition to fish feed formulation is, therefore, needed to prevent the accumulation of heavy metals in the fish's body. Hence, an experiment using a Completely Randomized Design (CRD) with 5 treatments and 3 replications was conducted to evaluate the performance and feed efficiency of Asian seabass Lates calcarifer juveniles with the addition of HA to their green mussel–based diet containing the heavy metal cadmium (Cd). Five experimental diets were formulated with different dosages of HA addition at 0, 400, 800, 1200, and 1600 mg kg⁻¹ of feed as treatments A, B, C, D, and E, respectively. The experimental diets were given to triplicate groups of 15 Asian seabass juveniles with an initial body weight of 4.30 ± 0.60 g over a 70-day cultivation period, feeding until satiation. The results showed that feed consumption, feed digestibility, protein retention, growth performance, and feed efficiency were significantly affected by HA addition in the fish diet. Among all treatments, HA addition of 1600 mg kg⁻¹ produced the best biological response from the Asian seabass. To conclude, the HA addition of 1600 mg kg⁻¹ into the diet was the best dosage, resulting in optimal growth performance and feed efficiency in Asian seabass. The highest dose of HA in Cd-contaminated feeds could reduce Cd content, but had not yet been able to eliminate Cd in fish meat. Therefore, green mussel meal, with HA addition as an alternative protein source, has potential as a feed additive for Cd-contaminated diets of Asian seabass juveniles.
Co-Authors DEDI JUSADI Dinar Tri Soelistyowati Gusti Ngurah Permana Harton Arfah Haryanti Haryanti Jr., Muhammad Zairin Khotimah, Fitriyah Husnul Mia Setiawati MUNTI YUHANA Rasidi, Rasidi Sari Budi Moria Sembiring Sri Nuryati