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Kualitas Buah Tomat pada Pertanaman dengan Mulsa Plastik Berbeda Setyorini, D; Indradewa, D; Sulistyaningsih, E
Jurnal Hortikultura Vol 19, No 4 (2009): Desember 2009
Publisher : Indonesian Center for Horticultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar

Kondisi lingkungan merupakan salah satu faktor yang memengaruhi pertumbuhan tanaman. Memodifikasikondisi lingkungan selain akan memengaruhi pertumbuhan tanaman juga dapat memengaruhi kualitas buah yangdihasilkan. Penelitian dilaksanakan di Kebun Percobaan dan Laboratorium Hortikultura, Fakultas Pertanian, sertaLaboratorium Pangan dan Gizi, Fakultas Teknologi Hasil Pertanian, Universitas Gadja Mada, Yogyakarta, pada bulanFebruari sampai Juni 2004. Rancangan yang digunakan adalah acak kelompok, 5 perlakuan warna mulsa plastikdengan 4 kali ulangan. Variabel pengamatan meliputi jumlah buah per tanaman, buah rusak per plot, berat per buah,diameter dan panjang buah, kekerasan buah, nilai kematangan buah, total padatan terlarut dalam buah, kandunganvitamin C buah, dan kandungan asam dominan buah. Hasil penelitian menunjukkan bahwa warna mulsa plastikberpengaruh terhadap parameter kualitas spesifik buah tomat. Penggunaan mulsa plastik merah dapat memperkecilpersentase buah rusak, namun menurunkan nilai koefisien kematangan buah, sedangkan penggunaan mulsa plastikbiru dapat meningkatkan kekerasan buah. Sementara itu, mulsa plastik hitam perak dapat meningkatkan kadar asamdominan yang cocok untuk budidaya tomat olahanABSTRACT. Setyorini, D., D. Indradewa, and E. Sulistyaningsih. 2009. Fruit Quality of Tomato Planted inDifferent Plastic Mulch. Environment conditions is one of important factor that may affect plant growth. Modificationof environment conditions will not only affect plant growth, but also fruit quality. This study was carried out in TheExperimental Garden and Horticultural Laboratory of College of Agriculture, Gadjah Mada University. The experimentwas set up in a randomize block design with 5 levels of treatments (plastic mulch color) and 4 replications. Parameterobserved were fruit number per plant, damaged fruit per plot, fruit weight, diameter and length of fruit, fruit firmness,coefficient of fruit ripening, total soluble solid, vitamin C, and dominant acid. The results showed that the color ofplastic mulch had a specific effect on tomatoes fruit quality parameter. The use of red plastic mulch reduce thepercentage of damage fruits but decreased the coefficient of fruit ripening. The blue plastic mulch had a significanteffect on increase fruit firmnes. Meanwhile, the silvery black plastic mulch could increase the dominant acid contentthat was suitable for growing processing tomatoes.

Cloning and Clone Analysis of GRA1 Gene from Local Isolate Toxoplasma gondii Tachyzoite Subekti, Didik T; Artama, W.T; Sulistyaningsih, E; Poerwanto, S.H; Sari, Y; Bagaskoro, F
Indonesian Journal of Animal and Veterinary Sciences Vol 13, No 1 (2008)
Publisher : Indonesian Animal Sciences Society

The GRA1 gene of Toxoplasma gondii encoding protein called GRA1 protein. GRA1 protein known to be immunogenic and essentialy involved in modification of parasitophorus vacoule which has role in immune evasion and virulency of organism. The local isolate of T. gondii is successfuly isolated and known as highly pathogenic isolate similarly as its RH strain. Unfortunately, the homology sequence of GRA1 gene between those isolate still unknown. The purpose of the research are to clone the GRA1 gene and to analyze the homology from pathogenic T. gondii isolate and RH strain. Tachyzoite of T. gondii was grown in mice peritoneum by intraperitoneal injection. Then, total mRNA was isolated and purified. cDNA was synthesized from mRNA and then amplified using F1 dan R1 primers to get clone of GRA1 from local isolate. Homology analysis was perform using several bioinformatic softwares. The result showed that cDNA of GRA1 from local isolate has 84% homologs with RH strain of T.gondii. However, when subsequently editing performed to parts of suspected non coding sequence of cDNA GRA1 to get CDS of GRA1, the homology was increase to 100% compare to CDS of GRA1 of RH strain. Key words: GRA1, Toxoplasma gondii, Clonning, Expression

ANALISIS IMUNOGENISITAS PROTEIN GRA1 DARI HASIL KLONING GEN GRA1 TAKIZOIT Toxoplasma gondii [Immunogenicity Analysis of GRA1 Protein Derived from Clone Bearing GRA1 Genes Collected from Toxoplasma gondii Tachyzoite] Subekti, Didik T; Artama, WT; Poerwanto, SH; Sulistyaningsih, E; Sari, Yulia
BERITA BIOLOGI Vol 11, No 1 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The study was aimed to analyze the immunogenicity of GRA1 protein derived from clone bearing GRA1 genes from local isolate of Toxoplasma gondii. Analysis of GRA1 protein translated from cDNA of GRA1 is very essential in prior to expressed the gene. Analysis of GRA1 protein derived from clone bearing GRA1 genes was performed using several bioinformatics software which are available as standalone or online software such as CLC Bio Workbench series, BioEdit, BESTORF, GENSCAN, FGENES, BepiPred 1.0, CTL Epitope Finder and SignalP. Translation coding sequences of GRA1 gene into GRA1 peptide sequences revealed 190 amino acids with molecular mass of GRA1 approximately 20.159 kD and isoelectric point at 4.43. GRA1 protein also identified several antigenic domains with six domains were known as epitopes for CD8+/cytotoxic lymphocyte and seven domain as epitopes for B lymphocyte. However, GRA1 protein was considered as good antigen but less immunogenic.

Kualitas Buah Tomat pada Pertanaman dengan Mulsa Plastik Berbeda D Setyorini; D Indradewa; E Sulistyaningsih
Jurnal Hortikultura Vol 19, No 4 (2009): Desember 2009
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v19n4.2009.p%p

Kondisi lingkungan merupakan salah satu faktor yang memengaruhi pertumbuhan tanaman. Memodifikasikondisi lingkungan selain akan memengaruhi pertumbuhan tanaman juga dapat memengaruhi kualitas buah yangdihasilkan. Penelitian dilaksanakan di Kebun Percobaan dan Laboratorium Hortikultura, Fakultas Pertanian, sertaLaboratorium Pangan dan Gizi, Fakultas Teknologi Hasil Pertanian, Universitas Gadja Mada, Yogyakarta, pada bulanFebruari sampai Juni 2004. Rancangan yang digunakan adalah acak kelompok, 5 perlakuan warna mulsa plastikdengan 4 kali ulangan. Variabel pengamatan meliputi jumlah buah per tanaman, buah rusak per plot, berat per buah,diameter dan panjang buah, kekerasan buah, nilai kematangan buah, total padatan terlarut dalam buah, kandunganvitamin C buah, dan kandungan asam dominan buah. Hasil penelitian menunjukkan bahwa warna mulsa plastikberpengaruh terhadap parameter kualitas spesifik buah tomat. Penggunaan mulsa plastik merah dapat memperkecilpersentase buah rusak, namun menurunkan nilai koefisien kematangan buah, sedangkan penggunaan mulsa plastikbiru dapat meningkatkan kekerasan buah. Sementara itu, mulsa plastik hitam perak dapat meningkatkan kadar asamdominan yang cocok untuk budidaya tomat olahanABSTRACT. Setyorini, D., D. Indradewa, and E. Sulistyaningsih. 2009. Fruit Quality of Tomato Planted inDifferent Plastic Mulch. Environment conditions is one of important factor that may affect plant growth. Modificationof environment conditions will not only affect plant growth, but also fruit quality. This study was carried out in TheExperimental Garden and Horticultural Laboratory of College of Agriculture, Gadjah Mada University. The experimentwas set up in a randomize block design with 5 levels of treatments (plastic mulch color) and 4 replications. Parameterobserved were fruit number per plant, damaged fruit per plot, fruit weight, diameter and length of fruit, fruit firmness,coefficient of fruit ripening, total soluble solid, vitamin C, and dominant acid. The results showed that the color ofplastic mulch had a specific effect on tomatoes fruit quality parameter. The use of red plastic mulch reduce thepercentage of damage fruits but decreased the coefficient of fruit ripening. The blue plastic mulch had a significanteffect on increase fruit firmnes. Meanwhile, the silvery black plastic mulch could increase the dominant acid contentthat was suitable for growing processing tomatoes.

Cloning and Clone Analysis of GRA1 Gene from Local Isolate Toxoplasma gondii Tachyzoite Didik T Subekti; W.T Artama; E Sulistyaningsih; S.H Poerwanto; Y Sari; F Bagaskoro
Jurnal Ilmu Ternak dan Veteriner Vol 13, No 1 (2008): MARCH 2008
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The GRA1 gene of Toxoplasma gondii encoding protein called GRA1 protein. GRA1 protein known to be immunogenic and essentialy involved in modification of parasitophorus vacoule which has role in immune evasion and virulency of organism. The local isolate of T. gondii is successfuly isolated and known as highly pathogenic isolate similarly as its RH strain. Unfortunately, the homology sequence of GRA1 gene between those isolate still unknown. The purpose of the research are to clone the GRA1 gene and to analyze the homology from pathogenic T. gondii isolate and RH strain. Tachyzoite of T. gondii was grown in mice peritoneum by intraperitoneal injection. Then, total mRNA was isolated and purified. cDNA was synthesized from mRNA and then amplified using F1 dan R1 primers to get clone of GRA1 from local isolate. Homology analysis was perform using several bioinformatic softwares. The result showed that cDNA of GRA1 from local isolate has 84% homologs with RH strain of T.gondii. However, when subsequently editing performed to parts of suspected non coding sequence of cDNA GRA1 to get CDS of GRA1, the homology was increase to 100% compare to CDS of GRA1 of RH strain. Key words: GRA1, Toxoplasma gondii, Clonning, Expression

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