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Tingkat Maturasi in vitro Oosit Kambing dalam Medium dengan Suplementasi Serum dan Albumin Sri Gustari; Ni Wayan Kurniani Karja; Yuke Rizky Amelia; Ian Kurniawan; Bayu Sulistyo
Jurnal Veteriner Vol 10 No 4 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (796.622 KB)

Abstract

The present study aimed to study the effect of ?maturation media? on maturation rate of goat oocytesafter in vitro maturation. Goat ovaries were collected from a slaughter house in Godean, Sleman. Immediatelyafter slaughter the ovaries were collected, rinsed with physiological NaCl three times then placed in aflask containing the NaCl solutions and hept at 36-370C before transportaion to the laboratory. Oocyteswere observed under stereo microscope and its quality was classified into A, B, and C. Oocytes in vitromaturaion (IVM) was performed in TCM-199 media suplemented with : a) 0.4 mg/ml bovine serum albumin(BSA); and b) 10% newborn calf serum (NCS) then incubated at 38.50C with 5% CO2 for 24-27 h. Followingthis, oocytes were observed under inverted microscope for first polar body extrusion, then stained withaceto-orcein in order to evaluate nuck or maturation. The nuclear maturation stages including : germinalvesicles (GV), germinal vesicles break down (GVBD), metaphase I, apaphase I, telophase I and metaphaseII, respectivelly. The overall results showed that 74-74%, 52-66.6% and 21.5-23.8% of oocytes quality A, B,and C reached maturation at metaphase II, respectivelly. There were no significant differences in oocytesmaturation using media supplemented with either BSA or NCS.
PENGARUH KERUSAKAN SPAREPART TERHADAP POWER PANCAR RADIO VHF A/G PORTABLE ADC DI PERUM LPPNPI AIRNAV CABANG PALEMBANG Wimatra, Ayub; Caesar Akbar, Muhammad; Sulistyo, Bayu
SIBATIK JOURNAL: Jurnal Ilmiah Bidang Sosial, Ekonomi, Budaya, Teknologi, Dan Pendidikan Vol. 3 No. 1 (2023): December
Publisher : Penerbit Lafadz Jaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54443/sibatik.v3i1.1784

Abstract

In the world of aviation, the tool used for communication between aircraft in the air with ATC (Air Traffic Controller) officers on the ground is VHF Air to Ground radio. This radio has a very vital role in the world of aviation therefore this tool must be able to function properly. Many aspects can affect damage to the VHF A/G radio and cause the transmitting power emitted by the radio to not allow pilots and air traffic controllers to communicate. Among others is the damage of spare parts on the VHF A/G radio that occurs at Perum LPPNPI Airnav Palembang Branch. This research is qualitative research. Data collection techniques using interviews, observation, and documentation. The results of the study prove that damage to spare parts on the VHF A/G radio can affect the transmitting power and cause communication between pilots and ATC officers at Sultan Mahmud Badaruddin II airport.