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Carcass and physical meat characteristics of thin tail sheep (TTS) based on calpastatin gene (CAST) (Locus intron 5 â exon 6) genotypes variation Dagong, Muhammad Ihsan Andi; Herman, R; Sumantri, C; Noor, R.R; Yamin, M
Indonesian Journal of Animal and Veterinary Sciences Vol 17, No 1 (2012)
Publisher : Indonesian Animal Sciences Society

The quality of sheep carcass is mostly determined by the total lean meat production, meat distribution on the carcass and the quality of meat. Calpastatin gene (CAST) is known to have an association with carcass and meat quality traits. The objective of this research was to identify the association between CAST polymorphisms and carcass characteristics in Thin Tail Sheep (TTS). Thirty three heads of sheep representing three genotypes of CAST (CAST-11, CAST-12 and CAST-22) were identified for carcass and meat characterisation. There was no significant difference between CAST polymorphisms with meat tenderness, pH, water holding capacity and cooking loss, neither with carcass weight and dressing percentage among genotypes. Shoulder proportion of CAST-11 genotype was larger than that of CAST-12 or CAST-22, but the lean meat proportion of CAST-22 genotype in shoulder, rack and loin were higher than those of CAST-11 but not different from CAST-12. The fat percentage of CAST-11 was the highest among the genotypes. CAST-22 genotype has higher lean meat percentage than the CAST-11. Variation in CAST gene could be used as marker assisted selection in sheep for higher lean meat proportion. Key Words: Calpastatin, Polymorphisms, Carcass Characteristics, Meat Quality, Thin Tail Sheep

Diversity Gen Growth Hormone (Gh) of Kacang Goat In Kota Gorontalo and Regency Of Bone Bolango (Province Of Gorontalo) Ilham, Fahrul; Safriyanto Dako, Safriyanto; Rachman, Agus Bahar; Dagong, Muhammad Ihsan Andi; Rahim, Lellah
Proceeding INTERNATIONAL SEMINAR IMPROVING TROPICAL ANIMAL PRODUCTION FOR FOOD SECURITY PROCEEDING INTERNATIONAL SEMINAR
Publisher : Proceeding INTERNATIONAL SEMINAR IMPROVING TROPICAL ANIMAL PRODUCTION FOR FOOD SECURITY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (331.594 KB)

ABSTRACTÂ Growth Hormone (GH) is a hormone produced by cells in the anterior lobe of the pituitarysomatrotop and formation process under the control of GH gene. One important function of thishormone is to help the process of tissue formation and metabolism of fat to meat forming. Thepurpose of this study was to determine the genetic diversity of genes GH Kacang goat in subpopulationsÂ of Kota Gorontalo and Regency of Bone Bolango. Blood samples were used for DNAextraction process in Centre of Biotechnology Laboratory University of Hasanuddin is 41 samples ofKacang goats with 21 samples from Kota Gorontalo city and 20 samples from Regency of BoneBolango. Genomic DNA was extracted using a kit DNA extraction Genjet Genomic DNA Extraction(Thermo Scientific) following standard protocol phenol-chloroform, amplified by the technique ofPolymerase Chain Reaction (PCR), and genotyping was done by Polymerase Chain ReactionRestrictionFragment Length Polymorphism (PCR-RFLP) using the restriction enzyme Hae III. Datawere analyzed descriptively by calculating the frequency of genotype, allele frequency, and degreeof heterozygosity. The results showed GH genotype frequencies for the genotypes AA and Â AB were2.45 and 97.5% respectively and the frequency of alleles A and B were Â 51.2 and 48.7% per centrespectively and the observed heterozygosity (Ho) and expected heterozygosity (He) were 0.97 and0.50 respectively. Based on the sub-population genotype frequencies obtained GH gene of Kacanggoat from Kota Gorontalo is 95.25 % for Â AA and 4.76% for AB, the frequency of allele A and B was52.3% 47.6%, observated heterozygosity (Ho) 0.95 and expected heterozygosity (He ) 0.51. GH genegenotype frequencies in Kacang goat from Regency of Bone Bolango is AB 100%, the frequency ofallele A and allele B 0.5 0.5, observation heterozygosity (Ho) of 1.00 and expectation heterozygosity(He) 0.51. Based on the results concluded GH gene Kacang goat from Kota Gorontalo and Regency ofBone Bolango is polymorphic so that it can be used as the basis for the implementation of theselection.Key Words: Genetic Diversity, Growth Hormone, Kacang Goat

The physiochemical properties of kefir using honey concentrations Andi Padauleng Meliani Anwar; Fatma Maruddin; Farida Nur Yuliati; Jamyang Tashi Wangdi; Muhammad Ihsan Andi Dagong
jurnal1 VOLUME 4 ISSUE 1, JUNE 2021
Publisher : Hasanuddin University Food Science and Technology Study Program

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/canrea.v4i1.376

Kefir has a sour taste and distinctive aroma. This condition affects the level of consumer acceptance. The level of consumer acceptance of kefir can be improved by adding a sweetener, namely honey. This study aims to determine the characteristics of kefir made from commercial liquid milk to total lactic acid, pH value, viscosity, organoleptic (taste and preference) panelists to kefir with the addition of honey. This study used a completely randomized design (CRD). The treatment of this research was 4 honey concentrations (5%, 7%, 9% and without the addition of honey (0%) as a control) and was repeated 3 times. Kefir addition of honey is made in the following way: the liquid milk is sterilized at 105oC for 5 minutes and then the sterile milk is cooled down to a temperature of about 40oC. After chilling, sterile milk was inoculated with 3% (v/v) pre-propagated kefir starter and incubated at 37oC for 24 hours. Furthermore, kefir was added with honey treatment with a concentration of 5%, 7% and 9% (v/v) respectively and homogenized. Kefir honey is carried out in a series of tests including total lactic acid, acidity (pH), viscosity, organoleptic (taste and preference). The results showed that the different use of honey kefir did not change the lactic acid content. Increasing use of honey concentration causes pH value, viscosity, sweetness, and preference to increase. The best use of honey concentration in making kefir is 9%.

Carcass and physical meat characteristics of thin tail sheep (TTS) based on calpastatin gene (CAST) (Locus intron 5 – exon 6) genotypes variation Muhammad Ihsan Andi Dagong; R Herman; C Sumantri; R.R Noor; M Yamin
Jurnal Ilmu Ternak dan Veteriner Vol 17, No 1 (2012): MARCH 2012
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The quality of sheep carcass is mostly determined by the total lean meat production, meat distribution on the carcass and the quality of meat. Calpastatin gene (CAST) is known to have an association with carcass and meat quality traits. The objective of this research was to identify the association between CAST polymorphisms and carcass characteristics in Thin Tail Sheep (TTS). Thirty three heads of sheep representing three genotypes of CAST (CAST-11, CAST-12 and CAST-22) were identified for carcass and meat characterisation. There was no significant difference between CAST polymorphisms with meat tenderness, pH, water holding capacity and cooking loss, neither with carcass weight and dressing percentage among genotypes. Shoulder proportion of CAST-11 genotype was larger than that of CAST-12 or CAST-22, but the lean meat proportion of CAST-22 genotype in shoulder, rack and loin were higher than those of CAST-11 but not different from CAST-12. The fat percentage of CAST-11 was the highest among the genotypes. CAST-22 genotype has higher lean meat percentage than the CAST-11. Variation in CAST gene could be used as marker assisted selection in sheep for higher lean meat proportion. Key Words: Calpastatin, Polymorphisms, Carcass Characteristics, Meat Quality, Thin Tail Sheep

Performance of Male Bali Catte at Different Age By Feed Concentrate Containing Cocoa Pulp Isdam Supriadi; Lellah Rahim; Ambo Ako; Renny Fatmyah Utamy; Muhammad Ihsan Andi Dagong
Hasanuddin Journal of Animal Science (HAJAS) Vol. 2, No. 1 (2020)
Publisher : Faculty of Animal Science, Hasanuddin University, Makassar, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/hajas.viNo 1.11751

The cocoa pulp is a slimy layer that surrounds the pieces of seeds, consisting of part of water and a layer of nutritional components which is quite high, including sucrose, glucose, and a little starch. The cocoa pulp, the by-product of cocoa production, has been used to avoid environmental pollution as an organic fertilizer. However, for livestock feed, the information is so limited. Therefore, the objective of this study was to determine the performance of male Bali cattle at different ages by feed concentrate containing cocoa pulp. The experiment was carried out in a completely randomized design consisting of 3 treatments and 3 replications so that it needed 9 male Bali cattle. The treatment consisted of A1 = male Bali cattle 2 years; A2 = male Bali cattle age 3 years; and A3 = Bali cattle male age 4 years, respectively. The parameter was feed consumption, performance, daily body weight gain (DWG), and feed consumption rate (FCR). The results showed that the average feed consumption, DWG, and FCR, showed significantly different (P<0.05) on feed concentrate containing cocoa pulp. The results indicated that 4 years of male Bali cattle was good growth performance compared to 2 years and 3 years by feed concentrate containing cocoa pulp.

Pemberdayaan Masyarakat Pengolah Gula Aren Menuju Kemadirian Ekonomi dan Berdaya Saing di Desa Lamondape Kecamatan Polinggona Kabupaten Kolaka Purbaningsih, Yuli; Rahman, Irsan; Neks Triani; Baba, Syahdar; Dagong, Muhammad Ihsan Andi; Hasrin; Zulkhar Naim; Aulia Uswa Noor Khasanah; Arizal Hatam; Muhammad Rizal; Gustia; Rahmat Ari. F; Ikra, Ikra; Sagista, Aminah; La Sisi
Jurnal Pengabdian Multidisiplin Vol. 3 No. 2 (2023): Jurnal Pengabdian Multidisiplin
Publisher : Kuras Institute & Scidac Plus

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51214/japamul.v3i2.676

Kegiatan perekonomian masyarakat tergantung sarana prasarana sebagai pendukung dan ketrampilan yang dimiliki. Kegiatan perekonomian masyarakat yang tidak stabil menimbulkan pendapatan masyarakat dibawah garis kemiskinan. Potensi sumberdaya alam dan potensi sosial ekonomi yang dapat dimanfaatkan untuk meningkatkan pendapatan masyarakat. Salah satu potensi sumber daya alam yaitu tanaman aren yang diolah menjadi gula aren. Mitra sasaran yang dilibatkan adalah Kelompok Pengolah Gula Aren Desa Lamondape (produktif secara ekonomi) dan Kelompok Karang Taruna Malitutue (non produktif secara ekonomi). Permasalahan kelompok pengolah gula aren yaitu pada bidang produksi, pemasaran dan manajemen usaha. Permasalahan kelompok karang taruna malitutue yaitu pada bidang pendidikan dan ekonomi. Kegiatan pemberdayaan Kosabangsa melakukan pelatihan dan pendampingan terkait perancangan model bisnis marketplace melalui pemanfataan teknologi digital, Model Kelembagaan Quadruple Helix, dan model pengembangan usaha melalui diversifikasi produk dan rekayasa cetakan serta kemasan. Hal ini dapat memberikan dampak peningkatan jumlah penjualan gula aren, peningkatan pendapatan pelaku usaha gula aren dan memperluas wilayah penjualan produk gula aren dan sebagai pengembangan Produk Unggulan Desa (PUD) khususnya untuk komoditi aren dan produk olahan gula aren. Upaya ini bertujuan untuk produktifitas pengolah gula aren dan kelompok karang taruna dalam rangka meningkatkan pendapatan menuju ekonomi mandiri serta memiliki daya saing.

PEMBERDAYAAN KELOMPOK TANI BUNGA LANTORO MELALUI PENERAPAN TEKNOLOGI PAKAN TERNAK DALAM PEMBUATAN SILASE DAN KONSENTRAT Ningtiyas, Weny Dwi; Pratiwi, Nita Adilla; Sanjaya, Muhammad Fahyu; Dagong, Muhammad Ihsan Andi; Iqbal, Iqbal
Jurnal Abdi Insani Vol 12 No 4 (2025): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v12i4.2245

Tantangan kelompok tani bunga lantoro di desa Ulidang, Kecamatan Tammerodo Sendana dalam memenuhi kebutuhan pakan ternak secara berkelanjutan kerap menjadi hambatan bagi para peternak, dalam mengoptimalkan produksi ternaknya. Masyarakat di Desa Ulidan masih bertani dan beternak secara konvensional serta belum memanfaatkan limbah-limbah pertanian dan peternakan secara optimal. Keterbatasan ketersediaan pakan berkualitas terutama pada musim kemarau serta kurangnya pengetahuan peternak mengenai teknik pengolahan pakan menyebabkan produktivitas ternak di wilayah ini belum optimal. Kegiatan ini berfokus pada teknologi pengolahan pakan untuk meningkatkan produksi ternak melalui pelatihan pemanfaatan limbah pertanian. Kegiatan ini melibatkan 19 anggota kelompok tani bunga lantoro di Desa Ulidang. Kegiatan pemberdayaan ini dimulai dengan sosialisasi, pelatihan, pendampingan dan evaluasi. Kegiatan pengabdian berlangsung dengan baik, dimana para peserta mengikuti pelatihan dengan antusias dan mampu mempraktikkan pembuatan silase dan konsentrat. Program pengabdian di Desa Ulidan mencakup penyuluhan, pelatihan, dan pendampingan pengolahan limbah menjadi pakan silase dan konsentrat, desain kemasan, serta strategi pemasaran. Peserta dilatih langsung dan didampingi untuk memahami proses produksi. Dengan dukungan pemerintah desa, program ini meningkatkan pemahaman dan keterampilan masyarakat, dibuktikan melalui hasil evaluasi pre-test dan post-test serta antusiasme tinggi peserta. Pelaksaanan kegiatan pengabdian ini mendapat respon yang positif dari masyarakat, transfer teknologi dan inovasi dari pelaksana sangat baik. Diharapkan semua kegiatan yang dilakukan pada kegiatan pengabdian memberikan manfaat yang lebih besar kepada masyrakat desa Ulidan.

Polymorphisms of IGF-1 Gene in Indonesian Local Goat Reared Under Smallholder Farmers in Sulawesi Region Rahim, Lellah; Dagong, Muhammad Ihsan Andi; Bugiwati, Sri Rachma Aprilita; Purnomo, Nurul
Jurnal Agripet Vol 24, No 1 (2024): Volume 24, No. 1, April 2024
Publisher : Faculty of Agriculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v24i1.28830

ABSTRACT. The objective of this study was to determine the allele frequency of IGF-1 in Kacang and Peranakan Ettawa goats in Indonesia. The DNA samples were extracted from the blood of 105 heads of goats and collected from the South Sulawesi and West Sulawesi provinces. The IGF-1 target was amplified using the PCR-RFLP method. Two allele variants (A and B) and three genotypes of the IGF-1 gene (AA, AB, and BB) have been identified in the local goat population. Allele frequencies of IGF-1 were A (0.81) and B (0.19) in the total population of local goats. Allele A is the most common allele in both the Kacang and Peranakan Ettawa goat populations, with the highest frequency found in the Kacang population (0.87). The most common genotype is genotype AA, with the highest frequency in the Kacang population (0.75). The observed and expected heterozygosity were 0.276 and 0.303, respectively. The population of local goats in these regions was in Hardy-Weinberg equilibrium. The conclusion of this research is that the IGF-1 gene in local goats in the Sulawesi region is polymorphic and this diversity information can be used for association studies with growth traits, litter size, and twinning rate.(Polimorpisme gen IGF-1 pada populasi kambing lokal Indonesia yang dipelihara oleh peternak kecil di daerah Sulawesi)ABSTRAK. Penelitian ini bertujuan untuk mengetahui frekuensi alel IGF-1 pada kambing Kacang dan Peranakan Ettawa di Indonesia. Sampel DNA diambil dari darah 105 ekor kambing dan dikumpulkan dari Provinsi Sulawesi Selatan dan Sulawesi Barat. Target IGF-1 diamplifikasi menggunakan metode PCR-RFLP. Dua varian alel (A dan B) dan tiga genotipe gen IGF-1 (AA, AB, dan BB) telah diidentifikasi pada populasi kambing lokal. Frekuensi alel IGF-1 adalah A (0,81) dan B (0,19) pada total populasi kambing lokal. Alel A merupakan alel yang paling banyak ditemukan baik pada populasi kambing Kacang maupun kambing Peranakan Ettawa, dengan frekuensi tertinggi terdapat pada populasi Kacang (0,87). Genotipe yang paling umum adalah genotipe AA, dengan frekuensi tertinggi pada populasi Kacang (0,75). Heterozigositas yang diamati dan diharapkan adalah 0,276 dan 0,303, masing-masing. Populasi kambing lokal di wilayah ini berada dalam keseimbangan Hardy-Weinberg. Kesimpulan yang dihasilkan adalah bahwa gen IGF-1 pada kambing lokal yang dipelihara oleh peternak di wilayah Sulawesi bersifat polimorfik dan informasi keragaman ini dapat digunakan untuk studi asosiasi dengan sifat pertumbuhan, litter size dan kelahiran kembar.

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