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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Global Medical and Health Communication Journal of Tropical Biodiversity and Biotechnology Biosaintifika: Journal of Biology & Biology Education Jurnal Kedokteran Hewan
SONY HERU SUMARSONO
School of Life Sciences and Technology, Bandung Institute of Technology, Jalan Ganeca 10, Bandung 40132
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Veterinary

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Hubungan Antara Reactive Oxygen Species (ROS), Superoxide Dismutase (SOD) dengan Protein α-Sinuklein-larut Air pada Batang Otak Tikus yang Diinduksi Rotenon Yulianti, Arief Budi; Sumarsono, Sony Heru; Ridwan, Ahmad; Yusuf, Ayda T
Global Medical & Health Communication (GMHC) Vol 3, No 2 (2015)
Publisher : Fakultas Kedokteran Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Abstrak Parkinson adalah salah satu penyakit neurodegeneratif dengan ganggunan gerak bila kematian neuron dopaminergik lebih dari 70 %. Paparan neurotoksin diduga menjadi penyebab terjadinya Parkinson sporadik. Tujuan penelitian ini adalah menentukan kondisi stres oksidatif pada batang otak tikus Wistar yang diinduksi rotenon. Metode: Penelitian eksperimental dengan menggunakan tikus Wistar jantan. Variabel bebas: kelompok tikus, lama perlakuan, waktu pengamatan, dan lokasi di batang otak. Variabel terikat: konsentari SOD, konsentrasi protein α-sinuklein-larut air, dan densitas ROS. Densitas ROS berbeda secara signifikan antara kelompok perlakuan (sig: 0,029), waktu pengamatan (sig: 0,0001), dan lokasi di batang otak (sig: 0,001). Konsentrasi SOD tidak berbeda secara signifikan antar kelompok perlakuan (sig:0,566), waktu pengamatan (sig:0,441) dan lokasi di batang otak (sig: 0,091). Konsentrasi protein α-sinuklein-larut air berbeda secara signifikan antar kelompok perlakuan (sig: 0,001), waktu pengamatan (sig: 0,001) tetapi tidak berbeda secara signifikan pada lokasi di batang otak (0,625). Densitas ROS relatif tertinggi pada hari ke-10 dan 40. Sementara itu konsentrasi SOD pada hari ke-10 dan 40 relatif rendah sedangkan konsentrasi protein α-sinuklein-larut air pada hari ke-10 relatif tinggi dibandingkan dengan hari ke-40. Dengan demikian dapat disimpulkan bahwa stres oksidatif pada batang otak tikus Wistar yang diinduksi rotenon berpengaruh pada struktur protein α-sinuklein.   Kata kunci: Batang otak, protein α-sinuklein, ROS, rotenon, SOD, stres oksidatif   Abstract Parkinson is the neurodegenerative disease with movement disordered, if the dopaminergic neurons dead more than 70%. Neurotoxins exposure is predicted cause sporadic Parkinson. The research aim is to determine oxidative stress stage in the brainstems Wistar rat’s treated-rotenone. Methods: An experimental study using male Wistar rats. The independent variable: groups of rats, long treatment, observation time, and location in the brainstem. The dependent variable: SOD concentration, concentration of protein α-synuclein-water soluble, and ROS density. ROS density significantly different among treatment groups (sig: 0,029), observation time (sig: 0.0001), and the location in the brainstem (sig: 0,001). SOD concentrations not significantly different among treatment groups (sig: 0.566), observation time (sig: 0.441) and the location in the brainstem (sig: 0.091). The concentration of protein α-synuclein-water soluble significantly different among treatment groups (sig: 0,001), observation time (sig: 0,001), but not significantly different at the location in the brainstem (0,625). ROS density relatively high at day 10 and 40. Meanwhile SOD concentration on day 10 and 40 are relatively low. And the concentration of α-synuclein protein-water soluble on day 10 is relatively higher than on day 40. The conclution is oxidative stress in the brainstem Wistar rat’s treated-rotenone effected on the protein α-synuclein structure Key word: α-synuclein protein, brainstem, oxidative stress, ROS, rotenone, SOD
Ekstrak Etanol Biji Pinang Muda (Areca catechu L) Meningkatkan Apoptosis pada Sel Testikular Mencit (Mus musculus L) Sumarsono, Sony Heru; Ayda T Yusuf, Eka Pasana Pujowati Indra Wibowo
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 3, No 1 (2018): February 2018
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (687.382 KB) | DOI: 10.24002/biota.v3i1.1890

Abstract

Biji pinang memiliki potensi sebagai bahan antifertilitas dan diduga dapat menginduksi apoptosis sel-sel spermatogenik. Penelitian ini membahas tentang perubahan jaringan testikular mencit akibat pemberian ekstrak etanol biji pinang muda. Ekstrak etanol biji pinang muda disiapkan dengan maserasi, penjemuran, perendaman dan evaporasi hingga diperoleh serbuk ekstrak etanol yang kemudian dilarutkan dalam larutan Gum Arab 1% sesuai dosis. Mencit jantan galur Swiss Webster (umur8 minggu, berat 30-35 gram) dibagi dalam 5 kelompok perlakuan: (1) K = Kontrol, (2) KP = Kontrol pelarut (Gum Arab 1%), (3) P1 = Perlakukan 1 (dosis 300 mg/kg berat badan (bb)), (4) P2= Perlakuan 2 (dosis 500 mg/kg bb), dan (5) P3=Perlakuan 3 (dosis 700 mg/kg bb), diberikan secara oral (gavage) selama 17 hari. Mencit ditimbang sebelum perlakuan dan setelah perlakuan. Mencit dibunuh pada hari ke 18 dengan dislokasi leher, testis diisolasi, ditimbang dan difoto untuk mengetahui penambahan berat dan ukuran. Preparat histologi sayatan testis disiapkan dengan pewarnaan HE dan TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) assay untuk mengamati apoptosis. Hasil pengamatan menunjukkan terjadi (1) penurunan berat relatif testis kelompok P3, (2) ukuran testis relatif lebih kecil pada kelompok perlakuan, (3) perbedaan struktur jaringan testis pada kelompok P2 dan P3, al: (a) jumlah tubulus seminiferus lebih sedikit, (b) diameter tubulus seminiferus lebih kecil, (c) jarak antar tubulus seminiferus lebih renggang, (d) penurunan jumlah sel testikular, dan (e) peningkatan jumlah sel yang mengalami apoptosis. Ekstrak etanol biji pinang menyebabkan peningkatan apoptosis dan memiliki potensi sebagai agen alami antifertilitas pria. 
Utilization of Oocytes Collected from Preserved Ovarian for In Vitro Production of Cat Embryos Eriani, Kartini; Boediono, Arief; Sumarsono, Sony Heru; Azhar, Al
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (560.139 KB) | DOI: 10.15294/biosaintifika.v10i1.13958

Abstract

Preservation of ovarian tissue from severely injured or dead valuable animals has the potential to preserve female germ cells of animals. The ability to mature and fertilize of oocytes from preserved ovary of endangered species will allow us to sustain genetic and global biodiversities. The aims of this study were to investigate the viability of oocytes collected from the preserved ovary and its potential utilization for the production of cat embryos followed by in vitro maturation and fertilization. Ovary was preserved immediately in phosphate buffer saline (PBS) at 4 °C for 24 or 48 hours. The quality and viability of oocytes after the maturation process were identified microscopically using aceto-orcein staining. Biological function of the oocytes was evaluated by using in vitro culture technique for the maturation and fertilization rate in CR1aa medium culture. The results showed that the percentage of oocytes collected from preserved ovary for 24 and 48 hours that remained at the stage of metaphase-II were 29.4% and 21.9% respectively. Fertilization rates produced in the IVF using oocytes collected from ovary preserved for 24 or 48 hours were significantly lower (30%) than that of unpreserved control (36.7%). In conclusion, female germ cells of cat ovary preserved at 4 °C in PBS for 2 days were still viable for in vitro fertilization and thus can be utilized for in vitro production of cat embryos. Information obtained can be used as a basis of knowledge of using a combination of physiological reagent and cold-based preservation technique in modern reproductive technology for animals.
Microbial Count and AvBD10 Expressions in Ovaries and Oviducts of Kampung Unggul Balitbangtan (KUB)-1 Chickens Following Intravaginally CpG-ODN and S. Enteritidis Suryohastari, Raden Rara Bhintarti; Sumarsono, Sony Heru; Giri-rachman, Ernawati Arifin; Edi, Suryo Purnomo; Sukoco, Rinto; Wicaksana, Dwi Nawang
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 2 (2024): June
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.88750

Abstract

Indonesia boasts diverse native chickens (Gallus gallus domesticus) known for more disease resistance in comparison to broiler chicken, and Kampung Unggul Balitbangtan (KUB)-1 is designated as Indonesia's superior breed. Salmonella Enteritidis (SE) is associated with salmonellosis, a foodborne illness that can be transmitted by transovarial, wherein colonisation in the oviduct ascends to the ovaries. However, studies mimicking transovarial salmonellosis via intravaginal treatment of chicken have been limited. Meanwhile, Cytosine-phosphate-guanine oligodeoxynucleotide (CpG-ODN) stimulation has been known to induce avian β-defensins (AvBDs). This in vivo study aimed to determine the effects of intravaginal CpG-ODN treatment and SE challenged on microbial count and AvBD10 expression regarding the potential of intravaginally CpG-ODN to enhance innate immunity as an alternative approach against transovarial Salmonellosis. A total of 39 KUB-1 chickens were divided into four groups: T1 (CpG-ODN treatment), T2 (SE treatment), T3 (CpG-ODN treatment + challenged with SE), and C (Control). Observation was carried out from day 1 to day 4 post-intravaginal (PI). We found a significant increase in ovarian microbial count (p≤0.05). Notably, ovaries and oviducts remained uncontaminated post-SE challenge. Intravaginal CpG-ODN treatment significantly upregulated AvBD10 in both ovaries (p=0.016) and oviducts (p=0.023). Therefore, KUB-1 chickens exhibit SE immunity, and intravaginal CpG-ODN administration holds promise for preventing transovarial Salmonellosis in laying hens. 
THE EFFECT OF COGON GRASS ROOT ETHANOL EXTRACT ON REPRODUCTIVE ORGAN AND EPIDIDYMAL SPERM QUALITY OF MOUSE WITH DIFFERENT AGES Sudiman, Jaqueline; Widyastuti, Rini; Lubis, Alkaustariyah; Hartady, Tyagita; Syamsunarno, Mas Rizky Anggun Adipurna; Sumarsono, Sony Heru
Jurnal Kedokteran Hewan Vol 14, No 1 (2020): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v14i1.14969

Abstract

This study aims to determine changes related to epididymal sperm parameters in mice given cogon grass root ethanol extract (CGREE). The experiment was carried out by dividing three groups of mice aged 4-week old (early pubertal), 8-week old (adult), and 24-week old (old) as control and treatment groups. The treatment groups were given 115 mg/kg body weight of CGREE for 14 days. The mice were sacrificed on day 15 to asses reproductive organ indexes and quality of the epididymal sperm. Index of epididymis and ductus deferens indices significantly reduced in the 4-week old treatment group compared to control (0.23 vs 0.33; 0.08 vs 0.16, respectively), and vesical seminal significantly reduced in 8- week old treatment group compared to control (0.76 vs 0.68). The epididymal sperm quality significantly decreased in all treatment groups, but the sperm concentration in early pubertal group showed an increase. These results indicate that CGREE can enhance several semen parameters in prepubertal and adult mice but this effect is not apparent in old mice.
PRODUKSI EMBRIO KUCING SECARA IN VITRO DARI SPERMATOZOA HASIL PRESERVASI MELALUI FERTILISASI MIKRO Eriani, Kartini; Sukra, Yuhara; Boediono, Arief; Djuwita, Ita; Sumarsono, Sony Heru
Jurnal Kedokteran Hewan Vol 7, No 1 (2013): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v7i1.563

Abstract

Penelitian ini bertujuan mengetahui motilitas dan kemampuan memfertilisasi sperma kucing yang berasal dari epididimis yang disimpanpada suhu 4 C. Epididimis disimpan dalam media phosphate buffer saline (PBS) pada suhu 4 C selama 1, 3, dan 6 hari. Viabilitas spermatozoa diamati dengan pewarnaan hoechst-propidium iodine. Fungsi biologis spermatozoa dievaluasi melalui teknik kultur in vitro dengan fertilisasi mikro dan perkembangan embrio di dalam media kultur CR1aa. Hasil penelitian menunjukkan persentase spermatozoa hidup pada hari ke-1, 3, dan 6 penyimpanan masing-masing adalah 81,0; 71,7; dan 70,7% (duktus deferens), 84,0; 81,2; dan 63,2% (kauda epididimis), 84,0%; 75,0; dan 74,7% (korpus epididimis). Persentase pronukleus (PN) yang didapat dengan teknik intra cytoplasmic sperm injection (ICSI) menggunakan spermatozoa epididimis pada hari ke-1, 3, dan 6 hari penyimpanan masing-masing adalah 8,0; 10,0; dan 5,9%. Preservasi epididimis pada suhu 4 C dalam PBS dapat digunakan untuk fertilisasi dan menghasilkan embrio kucing secara in vitro.
Co-Authors Ahmad Ridwan Al Azhar Arief Boediono Arief Budi Yulianti Ayda T Yusuf, Ayda T Ayda T Yusuf, Eka Pasana Pujowati Indra Wibowo Edi, Suryo Purnomo ERNAWATI ARIFIN GIRI-RACHMAN ITA DJUWITA KARTINI ERIANI Lubis, Alkaustariyah Mas Rizky A.A. Syamsunarno Rini Widyastuti Rinto Sukoco Sudiman, Jaqueline Suryohastari, Raden Rara Bhintarti Tyagita Hartady Wicaksana, Dwi Nawang Yuhara Sukra