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Neurogenic Differentiation of Bone Marrow Mesenchymal-Like Stem Cell Induced by Delonix regia Flowers Extract Ichsan, Ichsan; Boediono, Arief; Eriani, Kartini; Suryani, Irma; Azhar, Al; Nursanti, Risa
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.15051

Stem cell technology has great potential in the effort to cure degenerative diseases. This study was done to determine optimum dose of flamboyant (Delonix regia) flower extract to induce proliferation and differentiation of mice (Mus musculus) bone marrow mesenchymal-like stem cell. Bone marrow cells were collected from mice by aspiration. Cells suspension (1 x 106) were poured into petri dishes containing 2 ml of modified Dulbeccos Modified Eagles Media (mDMEM) and incubated overnight at 37 Â°C in a 5% CO2 incubator and microscopically observed. In quadriplicate, MSC were cultured in mDMEM containing D. regia flower extract of 0.0 (control), 0.4, 0.6, 0.8, and 1.0 mg/ml and incubated at 37 Â°C for 9 days. Population doubling time (PDT) and differentiated cell type were microscopically observed using HE staining on day 1 and 10. Data obtained were analyzed by ANOVA and Tukey test. The results showed that the addition of D regia flowers extracts 0.8 and 1.0 mg/ml significantly reduced PDT compared to that of 0.4, 0.6 and control. The extract, at 0.4 and 0.6 mg/ml, were able to induce MSC differentiation into fibroblast-like and nerve-like cells. In conclusion, D. regia flower extracts of 0.6, 0.8 and 1.0 mg/ml were able to stimulate MSC proliferation, but optimum dose for neurogenic differentiation was 0.6 mg/ml. This is the first to show potential of D. regia flower extract as neurogenic differentiatian inducer on mice MSC. These findings can be used as preliminary information for using the extract as cellular differentian inducer in basic and applicative reseach using stem cells.

Utilization of Oocytes Collected from Preserved Ovarian for In Vitro Production of Cat Embryos Eriani, Kartini; Boediono, Arief; Sumarsono, Sony Heru; Azhar, Al
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Preservation of ovarian tissue from severely injured or dead valuable animals has the potential to preserve female germ cells of animals. The ability to mature and fertilize of oocytes from preserved ovary of endangered species will allow us to sustain genetic and global biodiversities. The aims of this study were to investigate the viability of oocytes collected from the preserved ovary and its potential utilization for the production of cat embryos followed by in vitro maturation and fertilization. Ovary was preserved immediately in phosphate buffer saline (PBS) at 4 °C for 24 or 48 hours. The quality and viability of oocytes after the maturation process were identified microscopically using aceto-orcein staining. Biological function of the oocytes was evaluated by using in vitro culture technique for the maturation and fertilization rate in CR1aa medium culture. The results showed that the percentage of oocytes collected from preserved ovary for 24 and 48 hours that remained at the stage of metaphase-II were 29.4% and 21.9% respectively. Fertilization rates produced in the IVF using oocytes collected from ovary preserved for 24 or 48 hours were significantly lower (30%) than that of unpreserved control (36.7%). In conclusion, female germ cells of cat ovary preserved at 4 °C in PBS for 2 days were still viable for in vitro fertilization and thus can be utilized for in vitro production of cat embryos. Information obtained can be used as a basis of knowledge of using a combination of physiological reagent and cold-based preservation technique in modern reproductive technology for animals.

Alkaline Phosphatase Expression From Mice Mesenchymal Stem Cells Induced By Flamboyant Flower (Delonix regia) Extract Eriani, Kartini; Anggraini, Deby; Bintoro, Yudha; Ichsan, Ichsan; Azhar, Al; Mariya, Silmi
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.25433

Flamboyant flower (Delonix regia) extract can increase proliferation and differentiation rates of mesenchymal stem cells (MSCs) into specific cells such as bone, nerve, and fibroblast cells. The extract possess metabolic compounds that may act as antibiotics, anti-inflammatory, antimicrobial, diuretic, anthelmentic, astringent, leucorrhoea, and potentially increase the body's metabolism normally. This study aimed to investigate expression level of alkaline phosphatase (ALP) by mice MSCs treated with flamboyant flower extract in vitro. Here, mice bone marrow cell cultures were treated with flamboyant flower extracts of 0.6 mg/ml (P1), 0.7 mg/ml (P2), 0.8 mg/ml (P3), and 0.9 mg/ml (P4). Untreated cell culture was used as negative control (P0). Expression of ALP gene was measured by RT-qPCR method. The results showed that mice mesenchymal stem cell could differentiate into bone, nerve, and fibroblast cells. The addition of flamboyant flower extract ranged from 0.6-0.9 mg/ml significantly (p<0.05) influenced the expression of ALP by differentiating MSCs. The highest expression was found at the stem cells treated with flamboyant flower extract of 0.8 mg/ml, 0.13 times compared with control. In conclusion, flamboyant flower extracts treatment might increase the expression of ALP in differentiating MSCs. Â This information can be used as a basis for finding an appropriate biomarkers for tracking the differentiation and profileration of tissue originated MSCs induced by extracts of medicinal plants.

Antidiabetic Potential of Methanol Extract of Flamboyant (Delonix regia) Flowers Eriani, Kartini; Hasanah, Uswatun; Fitriana, Rizky; Sari, Widya; Yunita, Yunita; Azhar, Al
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 2 (2021): August 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i2.30080

Providing scientific evidences for the medicinal benefits and possible toxic effects of the flamboyant flowers (Delonix regia (Bojer ex Hook.) Raf.) are very important to implementing the medicinal plant in this modern era. This study aimed to investigate antidiabetic potential of methanol extract of flamboyant flowers using a completely random design. Twenty-four male rats were randomly divided into 6 treatment groups with 4 replications: healthy rats (normal control, P1), diabetic rats treated with flamboyant flower extract of 0 (negative control, P2), 100 (P3), 200 (P4), and 400 mg/kg BW (P5), and rats which were induced by 0.45 mg/kg BW of Glibenclamide (positive control, P6). Diabetic condition was achieved by a single injection of alloxan 150 mg/kg BW. Treatments were given once a day for 14 days. On day 0, 3 and 18 blood samples were withdrawn from ratsâ€™ orbital vein for glucose measurement. All rats were sacrificed for liver, gastrocnemius muscles and pancreatic tissues collection. The liver and gastrocnemius muscle were subjected for glycogen measurement whereas pancreatic tissues were processed for histological examinations. Data was analyzed by ANOVA and followed by Duncan test. The results showed that flamboyant flowers extracts significantly (p<0.05) reduced blood glucose as well as degeneration and necrosis of pancreatic Î² cells. Optimal dose to decrease blood glucose and pancreatic cell degeneration was 200 mg/kg BW; whereas optimum dose to decrease pancreatic cell necrosis was 400 mg/kg BW. In conclusion, flamboyant flower extract can reduce blood glucose in rats. This is the first that shows antidiabetic potential of local Flamboyant flower extracts along its toxicity effect to pancreatic tissues. These information could become a basic consideration for the use of the plant extracts as a candidate to cure patients with diabetic problems.

Covid-19 Pandemic: Impact on skill improvement of the Indonesian teachers in using online learning applications Arif, Zuardin; Azhar, Al
ELS Journal on Interdisciplinary Studies in Humanities Vol. 4 No. 4 (2021): DECEMBER
Publisher : Hasanuddin University

The Covid-19 outbreak was first confirmed to have infected 2 Indonesians on March 2, 2020. Indonesian schools began to close in mid-March, face-to-face teaching, and the learning process turned into online learning. Since then, the use of online learning applications increased significantly. The objective of this study was to investigate the benefits of using online learning applications for Indonesian teachers. Data collection by distributing online questionnaires in Google form to teachers joining online training, online exam preparation questions organized by the LPPPTK KPTK, opened the link on May 14 and closed on May 27, 2020. 911 participants giving responses came from 32 provinces of 34 in Indonesia. Data were processed with SPSS software version 26.0. A univariate analysis was chosen to describe the frequency distribution and percentage of each variable. Spearman's correlation coefficient, r, was used to evaluate the association among the variables, and a two-tailed p < 0.05 was considered the statistically significant relationship between sample characteristics, the benefit of online training substance, and the developing teacher skills in using several online learning applications during the Covid-19 pandemic. Respondent areas mapping used geographic information system (GIS), assisted by ArcGIS 10.3 software. The results confirmed that 748 (82.11%) teachers said that more learning applications were mastered during the pandemic compared to before Covid-19 infection. The gender, education, school level, and region didn’t influence skill improvement in using online learning applications. But it was largely determined by the online training substance, the significance value < 0.05, and the degree of relationship 0.90.

Transformation of Jihad education strategies: Overcoming the challenges of Radicalism and Terrorism Darsimon, Darsimon; Azhar, Al
International Journal on Advanced Science, Education, and Religion Vol 7 No 2 (2024): IJoASER (International Journal on Advanced Science, Education)
Publisher : Sekolah Tinggi Agama Islam Al-Furqan, Makassar - Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33648/ijoaser.v7i2.650

Terrorism is often associated with Islam. As a result, quite a few of those who do not understand Islamic teachings are influenced and see Islam as a religion that is synonymous with violence. One of the things that underlies this view is the concept of jihad. Jihad is considered synonymous with the struggle to take up arms, by spreading terror everywhere. Even though it is contrary to the values of Islamic teachings. Facing this, Muslims must make efforts to rectify and raise awareness among the public about true jihad according to Islamic teachings. This is done through jihad education at both formal and informal levels. Formally, jihad education can be included in one of the learning curricula or lectures, especially in Islamic schools such as Islamic boarding schools, Islamic boarding schools, Tsanawiyah madrasas, Aliyah madrasas, or Islamic higher schools. Meanwhile, informally, an active role and synergy of all components, especially government, academics, ulama, including the mass media, are needed. All lead to the goal of building public awareness about the nature of jihad according to Islamic teachings.

Perubahan tutupan dan pemanfaatan lahan di Pulau Kaledupa, Taman Nasional Wakatobi, Sulawesi Tenggara Azhar, Al; Anggoro, Ari
Jurnal Oase Nusantara Vol 2 No 1 (2023)
Publisher : Balai Pengembangan Penjaminan Mutu Pendidikan Vokasi Bidang Kelautan Perikanan Teknologi Informasi dan Komunikasi

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Tujuan studi ini adalah mendeteksi LULCC (land use land cover change) di daratan pulau Kaledupa dari tahun 2002 sampai 2016, melihat pola LULCC berdasarkan ruang di setiap tingkatan teras pulau, dan menilai tingkat heterogenitas spasial di Pulau Kaledupa. Data citra yang digunakan adalah Landsat 7ETM+ 25 Oktober 2002, Landsat 5TM 06 Februari 2009, dan Landsat 8 OLI 05 September 2016. Pra pengolahan citra mengacu kepada Ardiansyah (2015), dilanjutkan Maximum Likelihood Classification (MLC) dengan menggunakan perangkat lunak ENVI 5.1. Model sederhana pulau (MSP) diterapkan untuk mendukung identifikasi LULC dan perubahannya pada setiap tingkatan teras terumbu Pulau Kaledupa menggunakan ArcMap 10 dan Microsoft Excel. Analisis heterogenitas dan konektivitas spasial menggunakan software Fragstats v.4.2.1. LULCC Pulau Kaledupa dari tahun 2002 hingga 2016 adalah hutan campuran terus mengalami peningkatan dari 3.132,64 ha menjadi 5.057,36; luas area pemukiman juga naik dari 246,87 ha menjadi 327.27 ha; sedangkan lahan terbuka terus menurun dari 4.462,55 ha menjadi 2.419,83 ha. Pemukiman penduduk menjadi salah satu pendorong LULCC di Pulau Kaledupa baik di daratan maupun di laut (1.26% tahun 2002 dan 1.69% tahun 2016). Heterogenitas spasial, mengalami fragmentasi dan perubahan yang cukup bervariasi. Kondisi tersebut mempengaruhi konektivitas spasial LULC. Pada metrik kelas, secara fungsi, penurunan nilai konektivitas (CONNECT) berkisar dari -0.07 hingga -2.36%, sedangkan secara struktur (COHESION) berkurang antara -1.78 hingga -2.36. Sementara itu, pada metrik lanskap, CONNECT memiliki penurunan nilai sebesar -0.35 dan COHESION -0.08.

Transformation of Jihad education strategies: Overcoming the challenges of Radicalism and Terrorism Darsimon, Darsimon; Azhar, Al
International Journal on Advanced Science, Education, and Religion Vol 7 No 2 (2024): IJoASER (International Journal on Advanced Science, Education)
Publisher : Sekolah Tinggi Agama Islam Al-Furqan, Makassar - Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33648/ijoaser.v7i2.650

Terrorism is often associated with Islam. As a result, quite a few of those who do not understand Islamic teachings are influenced and see Islam as a religion that is synonymous with violence. One of the things that underlies this view is the concept of jihad. Jihad is considered synonymous with the struggle to take up arms, by spreading terror everywhere. Even though it is contrary to the values of Islamic teachings. Facing this, Muslims must make efforts to rectify and raise awareness among the public about true jihad according to Islamic teachings. This is done through jihad education at both formal and informal levels. Formally, jihad education can be included in one of the learning curricula or lectures, especially in Islamic schools such as Islamic boarding schools, Islamic boarding schools, Tsanawiyah madrasas, Aliyah madrasas, or Islamic higher schools. Meanwhile, informally, an active role and synergy of all components, especially government, academics, ulama, including the mass media, are needed. All lead to the goal of building public awareness about the nature of jihad according to Islamic teachings.

Dampak Komunikasi Digital: Analisis Media Konvensional Ditaklukkan Media Baru Dikalangan Mahasiswa Ilmu Hubungan Internasional Universitas Almuslim Azhar, Al; Iskandar, Umar
Lentera : Jurnal Ilmiah Sains, Teknologi, Ekonomi, Sosial, dan Budaya Vol. 8 No. 4: LENTERA, DESEMBER 2024
Publisher : LPPM Universitas Almuslim

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Media has undergone many changes, from the existence of traditional media, through media convergence to the emergence of new media today. Along with the increasing complexity of technology and the easier access to new media, traditional media is slowly being eliminated from the market. Students are actively using digital media. What are the differences in the use of traditional media and new media among students of the International Relations Study Programme, Faculty of Social and Political Sciences, Almuslim University. Then, what are the responses and critical reactions that students have to communication in the current digital era? The purpose of this study is to determine the differences in the use of traditional communication media and new communication media among students of the International Relations Study Program class of 2024. The research methodology used in this study is a qualitative methodology that involves descriptive description through data analysis techniques in the form of interviews and field surveys (observation). The findings of this study indicate that the emergence of new media in today's digital era has both positive and negative impacts.

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