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All Journal Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Hemera Zoa Media Veteriner Jurnal Sain Veteriner ARSHI Veterinary Letters Jurnal Medika Veterinaria
Titiek Sunartatie
Division Of Medical Microbiology, School Of Veterinary Medicine And Biomedical Sciences, IPB University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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Eksplorasi Antibakteri dari Kapang Tanah Arboretum Rizal Dwinto Rochman; Titiek Sunartatie; Usamah Afiff
Jurnal Ilmu Pertanian Indonesia Vol. 25 No. 3 (2020): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.25.3.456

Abstract

Research to discover new antibacterial agent has been increasing due to the increasing number of pathogenic bacteria become antibiotic-resistant. Microbiomes in soil mold have antibacterial potency by producing secondary metabolites. The aim of this research was to isolate soil molds from the Faculty of Forestry Arboretum at IPB University. Arboretum’s soil was sampled ± 7.5 g from several points with maximum depth of 15 cm from surface. Mold isolate were cultivated for 28 days using waterbath shaker. Cultivated mycelium and soil filtrate were then separated and extracted using different methods and solvents, prior to antibacterial activity test using agar well diffusion method. Six isolates from Aspergillus, Penicillium, Paecilomyces, and Verticilium were used in this research. Cultivatation using sabouraud dextrose broth (SDB) contains pepton and dextrose in 25°C capable of stimulating mold growth. The research showed the result of secondary metabolit had antibacterial characteristic toward tested bacteria. The results of antibacterial activity test on both mycelium and soil filtrate extract showed that soil mold isolates had antibacterial activity against Staphylococcus aureus and Escherichia coli. Isolate 103A originated from soil filtrate formed the largest inhibition zone on both tested pathogens. Keywords: antibacteria, mycelium, soil filtrate, soil mold
PF-27 Incidence of Feline Dermatophytosis at Dramaga, Bogor in 2013-2018 Agustin Indrawati; Titiek Sunartati; Handina Rakhmawati
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (489.126 KB)

Abstract

Indonesia has a tropical climate and high humidity that give a high prevalence for fungal infections in cat skin such as Feline dermatophytosis (ringworm). Dermatophytosis is the most common fungal infection of cats and one of the most important infectious skin diseases in this species. It may be transmitted to other animal species and also contangious nature, zoonotic potential (Moriello, 2014). Feline dermatophytosis cases are caused by genera Microsporum, Trichophyton, and Epidermophyton. All of these agents produce proteolytic and keratolytic enzymes that enable them to utilize keratin as the sole source of nutrition after colonization of the dead, keratinized portion of epidermal tissue (mostly stratum corneum and hairs, sometimes nails) (Mattei et al., 2014). The aim of this study are to identified and evaluate the kind of fungal that can cause feline dermatofitosis in Dramaga in 2013 until 2018.
The Inhibitory Effect of Aflatoxin on The Growht of Bacillus megaterium and Staphylococcus epidermidis Eko Sugeng Pribadi; Unang Patriana; Titiek Sunartatie
Media Veteriner Vol. 5 No. 1 (1998): Media Veteriner
Publisher : Media Veteriner

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (451.653 KB)

Abstract

B. megaterium and S. epidermidis were used to study the inhibitory effect or pure aflatoxin and aflatoxin-contaminated rice. Results showed that the inhibitory effect was more pronounced In B. megatarium growth. The inhibitory effect of pure aflatoxin started at the concentration of 20 μg/ml and persisted to the concentration of 25 μg/ml (P
Trichophyton mentagrophytes sebagai Agen Penyebab Dermatofitosis pad Kambing Titiek Sunartatie
Jurnal Sain Veteriner Vol 28, No 1 (2010): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2496.292 KB) | DOI: 10.22146/jsv.459

Abstract

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Sensitivitas antimikroba dari bakteri terisolasi yang paling umum pada Feline Upper Respiratory Infection Karen Lee; Usamah Afiff; Safika Safika; Titiek Sunartatie
ARSHI Veterinary Letters Vol. 5 No. 3 (2021): ARSHI Veterinary Letters - Agustus 2021
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.5.3.55-56

Abstract

Pada kucing, infeksi saluran pernapasan atas (URI) dapat dikaitkan dengan infeksi bakteri primer atau sekunder dan umumnya dilakukan pengobatan dengan antimikroba. Penggunaaan antimikroba yang tidak tepat, dan penggunaannya berlebihan. Tidak ada protokol rinci untuk pengobatan, seperti yang tersedia untuk pengobatan manusia. Tujuan penelitian ini adalah untuk mengetahui kepekaan antimikroba dari bakteri yang diidentifikasi pada kucing yang terinfeksi URI. Dilakukan isolasi dan identifikasi bakteri pada sampel kucing yang terinfeksi URI kemudian dilanjutkan dengan uji kepekaan antibiotik amoksisilin, doksisiklin, tetrasiklin, azitromisin, siprofloksasin, dan sefotaksim dengan metode difusi Kirbey Bauer Agar Dics. Berdasarkan hasil penelitian, bakteri yang dapat diidentifikasi adalah Enterobacter spp, Streptococcus spp, Staphylococcus spp, Pseudomonas spp, Seratia spp, Yersinia spp, Micrococcus spp, Klebsiella spp dan Hafnia spp. Ditemukan resistansi antibiotik amoksisilin pada empat isolat Staphylococcus spp dan dua isolat resistan terhadap sefotaksim. Resistansi antibiotik amoksisilin dan tetrasiklin ditemukan pada satu isolat Streptococcus spp, dua isolat resistan terhadap sefotaksim serta dua isolat intermediet terhadap doksisiklin. Satu isolat bakteri Enterobacter spp resistan terhadap amoksisilin, azitromisin dan dua isolat resistan terhadap sefotaksim. Semua isolat yang diuji sensitif terhadap siprofloksasin. Berdasarkan uji kepekaan antibiotik, sebagian besar isolat bersifat sensitif, namun terdapat ada isolat yang resistan terhadap antibiotik, terutama amoksisilin dan sefotaksim.
Bacterial Identification of Ornamental Betta Fish (Betta splendens) Safika Safika; Nazmi Zahir B. Mohd Zaini; Usamah Afif; Agustin Indrawati; Rahmat Hidayat; Titiek Sunartatie
Jurnal Medika Veterinaria Vol 17, No 1 (2023): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v17i1.29323

Abstract

Betta fish (Betta splendens) are one of the most popular ornamental fish due to their gorgeous colors and various shapes. This study aimed to find pathogenic bacteria in betta fish (Betta splendens) in fish farm in Bogor. Body swabs were collected from four decorative betta fish, and from tank water. Two of the fish were dead and two of which were exhibiting symptoms of disease. Bacterial isolation was performed on multiple agar media, and bacteria were identified based on the growth characteristics of the colonies, Gram staining, and biochemical testing. The results of the analysis revealed the presence of 16 bacterial isolates, including Edwardsiella sp., Escherichia coli, and Vibrio sp. from dead fish, whereas Citrobacter sp, Providencia sp, and Vibrio sp were detected in diseased fish. In aquarium water, Escherichia coli, Citrobacter sp Proteus sp and Providencia sp. were detected. Betta fish are susceptible to suffer from diseases if the water quality is low since maintaining and enhancing the health of betta fish is highly dependent on water quality.
Identifikasi Bakteri Pencernaan dan Uji Resistansi pada Primata di Kebun Binatang Bukittinggi safika, safika; Indrawati, Agustin; Hidayat, Rahmat; Afiff, Usamah; Sunartatie, Titiek; Nauval Firdana, Chorrysa; Destri Prameswari, Alvira
Acta VETERINARIA Indonesiana Vol. 11 No. 3 (2023): November 2023
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.11.3.196-203

Abstract

Bakteri merupakan satu di antara penyebab terjadinya beberapa penyakit infeksi. Jenis bakteri yang dapat menginfeksi tubuh berbeda-beda tergantung organ atau lokasi target. Organ yang sering diinfeksi oleh bakteri adalah saluran pencernaan. Penelitian ini bertujuan mengidentifikasi jenis bakteri saluran pencernaan dan pola resistansi bakteri terhadap beberapa jenis antibiotik. Feses primata diperoleh dari Kebun Binatang Bukittinggi yang diisolasi pada media Eosin Methylene Blue Agar (EMBA), MacConkey agar (MCA), dan agar darah. Isolat bakteri yang didapat kemudian diuji dengan pewarnaan Gram, dan uji biokimia untuk diidentifikasi. Bakteri Escherichia coli dan Staphylococcus aureus yang didapat dilakukan uji resistansi dengan metode disk Kirby Bauer. Jenis bakteri Gram negatif yang dapat diidentifikasi yaitu Shigella sp., Proteus sp., Escherichia coli, Klebsiella oxytoca, dan Yersinia sp., serta bakteri Gram positif yaitu Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus sp., Stomatococcus mucilaginosus, dan Bacillus sp.. Bakteri E. coli mengalami resistan terhadap antibiotik ampisilin, streptomisin, eritromisin (2 isolat dari 2 isolat), amoksisilin tetrasiklin, oksitetrasiklin, doksisiklin, gentamisin, kloramfenikol dan asam nalidiksat (1 isolat dari 2 isolat), sedangkan bakteri S. aureus hanya mengalami resistansi terhadap antibiotik ampisilin dan amoksisilin (1 isolat dari 1 isolat). Resistansi tersebut dapat terjadi karena penggunaan antibiotik yang tidak tepat, perpindahan gen antarmikroorganisme.
Identification and antibiotic resistance patterns of Klebsiella sp. isolated from the feces of Bornean Orangutans (Pongo pygmaeus) at Taman Safari Indonesia, Bogor Nora Dyah Ayu Purnamaningrum; Usamah Afiff; Titiek Sunartatie; Safika Safika; Rahmat Hidayat; Supratikno Supratikno; Yohana Tri Astuti
ARSHI Veterinary Letters Vol. 8 No. 2 (2024): ARSHI Veterinary Letters - May 2024
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.8.2.39-40

Abstract

Orangutans are the only great apes that still survive in the Southeast Asian Region. However, their population in the wild habitat continues to decline, with estimates indicating a 50% decreased over the last 60 years. There is limited microbiological data on the antibiotic resistance of Klebsiella sp. in the feces of Bornean Orangutan (Pongo pygmaeus). This study aimed to identify the presence of Klebsiella sp. and assess its resistance pattern to various antibiotics in the feces of Bornean Orangutans. The research involved isolation, identification, and antibiotic resistance testing. Results showed that three isolates (37.5%) were positive for Klebsiella sp. Sensitivity test revealed two distinct resistance patterns among Klebsiella sp. isolates from the feces of healthy P. pygmaeus in Taman Safari Indonesia, Bogor. These isolates exhibited resistance to β-lactam antibiotics but remained sensitive to aminoglycoside, tetracycline, and fluoroquinolone antibiotics.
Antibiotics resistance patters of Pasteurella multocida isolation from cattle Afiff , Usamah; Safika; Sunartatie , Titiek; Leng , Ang Jia
ARSHI Veterinary Letters Vol. 9 No. 1 (2025): ARSHI Veterinary Letters - February 2025
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.9.1.27-28

Abstract

This study aimed to evaluate the efficacy of multiple antibiotics, including oxytetracycline, enrofloxacin, gentamicin, ampicillin, and erythromycin, against Pasteurella multocida isolates from cattle. Antibiotic susceptibility was assessed using the disk diffusion method. The results revealed consistent sensitivity of P. multocida to enrofloxacin and gentamicin, whereas three of the five isolates remained susceptible to oxytetracycline. In contrast, all isolates exhibited resistance to erythromycin and ampicillin. Notably, Type A strains displayed higher resistance to oxytetracycline than Type B strains, potentially due to differential selective pressure. These findings underscore enrofloxacin and gentamicin as the most effective therapeutic options for hemorrhagic septicemia in cattle, given their robust antibacterial activity against P. multocida.
Co-Authors Afiff , Usamah Agustin Indrawati Destri Prameswari, Alvira Handina Rakhmawati Karen Lee Leng , Ang Jia Nauval Firdana, Chorrysa Nazmi Zahir B. Mohd Zaini Nora Dyah Ayu Purnamaningrum Rahmat Hidayat Rahmat Hidayat Rahmat Hidayat Rizal Dwinto Rochman Safika S, Safika Supratikno Supratikno Unang Patriana Usamah Afif Yohana Tri Astuti