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All Journal Jurnal Ilmu Dasar BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek)
Titin Supriatun
Jurusan Kimia FMIPA Universitas Padjadjaran
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Control & Systems Engineering Environmental Science Mathematics

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Journal : BERITA BIOLOGI

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PENGARUH 2,4-D DAN BA TERHADAPINDUKSIKALUS EMBRIOGENIK PADA KULTUR MERISTEM JAHE {Zingiber officinale Rose.) Sitinjak, Rama Riana; Rostiana, Otih; Karyono, Karyono; Supriatun, Titin
BERITA BIOLOGI Vol 8, No 2 (2006)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v8i2.2037

Abstract

The aim of this study was to evaluate the effects of 2,4-dichlorophenoxyacetic acid (2,4-D) and N -benzyladenin (BA) to embryogenic callus induction of meristem culture in ginger {Zingiber officinale Rose). Completely Randomixed Design was applied using factorial pattern, replicated 3 times. Two factors were assigned i.e: 2,4-D (1.0, 2.0 and 3.0 mg/L) and BA (0.0, 1.0, 3.0 and 5.0 mg/L). Result showed that 2,4-D and BA significantly affected the embryogenic callus induction. The highest embryogenic callus (93.33%) was achieved on MS (Murashige & Skoog) containing combination of 1.0 mg/L 2,4-D and 3.0 mg/L BA, after 8weeks incubation.Histological evaluation informed that the incubation of embryogenic calli started from cortex cells adjacent the meristem vascular tissues.
Co-Authors Asep Zainal Mutaqin Ida Indrawati Karyono Karyono Mohamad Nurzaman Octivia Trisilawati Otih Rostiana Rahmawati, Fitryasari Rama Riana Sitinjak Ruly Budiono, Ruly Sugarti, Dini Tia Setiawati