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All Journal BIOMA : Jurnal Biologi Makassar Bioma : Jurnal Biologi dan Pembelajaran Biologi BioEksakta : Jurnal Ilmiah Biologi Unsoed Jurnal Riset Rumpun Matematika dan Ilmu Pengetahuan Alam (JURRIMIPA) Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Cakrawala Pendidikan dan Biologi (jucapenbi)
Rizki Amelia Nasution
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Health Professions Immunology & microbiology Mathematics Veterinary Other

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KELIMPAHAN KOLONI BAKTERI Vibrio sp. BERDASARKAN LOKASI BUDIDAYA TAMBAK UDANG DI KABUPATEN PIDIE Zahratul Idami; Rizki Amelia Nasution
BIOMA Vol 5, No 2 (2020): BIOMA:JURNAL BIOLOGI DAN PEMBELAJARAN BIOLOGI
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32528/bioma.v5i2.4012

Abstract

Penelitian ini bertujuan untuk mengisolasi dan menghitung kelimpahan koloni bakteri Vibrio sp. dari air tambak udang dengan 5 lokasi yang berbeda di Kecamatan Simpang Kabupaten Pidie, Aceh. Penelitian ini menggunakan metode eksploratif dan pengambilan sampel menggunakan purposive sampling. Isolasi bakteri menggunakan media selektif TCBSA. Perhitungan kelimpahan koloni Vibrio sp. menggunakan metode Total Plate Count. Hasil penelitian menunjukkan bahwa kelimpahan bakteri Vibrio sp. di Kecamatan Kembang Tanjong untuk kelima kategori lokasi tambak tergolong aman (104 cfu/ml) yaitu 6,2 x 102 – 2,6 x 103 cfu/ml. Berbeda halnya dengan tambak di Simpang Tiga yang masih dapat dikatakan aman hanya tambak K2T2 (2,9 x 103 cfu/ml), K4T2 (2,2 x 104 cfu/ml, K2T1; K5T; K5T2 (2,3 x 104 cfu/ml) karena kelimpahannya 8,34 x 104 cfu/ml, sedangkan bakteri Vibrio lainnya sudah bersifat patogen (105 cfu/ml). Hasil pengukuran kualitas air tambak menunjukkan rata-rata nilai salinitas 10,67-30,33 ppt, suhu 27,9-33,8 oC, pH air 7,65-8,45, DO 5,35-8,42 dan kecerahan 20-55 cm. Tidak adanya hubungan korelasi yang signifikan dari kualitas air tambak terhadap kelimpahan koloni bakteri Vibrio sp.
UJI AKTIVITAS MINYAK ATSIRI DAUN EKOR KUCING (Acalypha hispida) SEBAGAI FUNGISIDA ALAMI TERHADAP JAMUR Sclerotium rolfsii DAN Fusarium oxysporum SECARA IN-VITRO Revina Yani Tarigan; Ulfayani Mayasari; Rizki Amelia Nasution
BIOMA : JURNAL BIOLOGI MAKASSAR Vol. 9 No. 1 (2024): Bioma : Januari - Juni 2024
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University

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Abstract

Penyakit busuk batang dan layu Fusarium merupakan penyakit yang disebabkan oleh jamur patogen yaitu Sclerotium rolfsii dan Fusarium oxysporum. Pengendalian dilakukan dengan pemberian fungisida alami minyak atsiri daun ekor kucing yang ramah lingkungan serta mengandung senyawa flavonoid, saponin, dan tannin. Penelitian ini bertujuan untuk mengetahui aktivitas fungisida alami minyak atsiri daun ekor kucing terhadap pertumbuhan jamur Sclerotium rolfsii dan Fusarium oxysporum secara in – vitro. Metode yang digunakan dalam pembuatan minyak atsiri daun ekor kucing menggunakan metode destilasi uap, serta uji pada aktivitas antijamur menggunakan metode difusi cakram dengan konsentrasi sampel dan kontrol positif 2,5%, 5%, 7,5%, dan 10%, untuk pembuatan produk fungisida alami minyak atsiri daun ekor kucing menambahkan larutan Tween 80. Berdasarkan hasil uji menggunakan Analysis of Variance (ANOVA) penelitian ini menunjukan adanya aktivitas antijamur fungisida alami minyak atsiri daun ekor kucing terhadap jamur Sclerotium roflsii dan Fusarium oxysporum dengan nilai signifikan p< 0.05. Hasil fungisida alami minyak atsiri daun ekor kucing menggunakan konsentrasi 2,5%, 5%, 7,5% dan 10% diperoleh hasil diameter zona hambat pada jamur Sclerotium roflsii yaitu 11,23, 7,6, 6,9 dan 6,0. Sedangkan hasil uji diameter zona hambat pada jamur Fusarium oxysporum 18,26, 19,96. 21,53 dan 22,53. Berdasarkan hasil dari rata – rata aktivitas antijamur zona hambat minyak atsiri daun ekor kucing dapat dijadikan sebagai fungisida alami terhadap jamur Sclerotium roflsii pada konsentrasi efektif 2,5%. Sedangkan jamur Fusarium oxysporum pada konsentrasi 2,5%. Kata Kunci: Minyak atsiri, Fungisida alami, Sclerotium roflsii dan Fusarium oxysporum Kata kunci : Minyak atsiri, Fungisida Alami, Sclerotium roflsii, Fusarium oxysporum
ISOLATION OF BACTERIA AS A BIOREMEDIATION AGENT FOR RECLAMATION OF MERCURY-CONTAMINATED SOILS Muhammad Idris; Rizki Amelia Nasution; Ulfayani Mayasari
Jurnal Bioteknologi dan Biosains Indonesia Vol. 10 No. 1 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

Gold mining commonly involves the use of mercury, which generates hazardous and toxic mercury-based waste, leading to soil contamination and adverse effects on human health. Bioremediation has been explored as a potential solution to this issue. This study aimed to identify indigenous microbial species with potential as bioremediation agents, assess their ability to influence chemical properties, and evaluate their effectiveness in reducing mercury content. The research was conducted in five stages: isolation of native bacteria, testing the potential of microorganisms, assessing the ability to reduce mercury, conducting soil tests, and identifying bacterial characteristics through microscopic and biochemical analyses. The results revealed three types of indigenous microbes, namely Pseudomonas, Neisseria, and Klebsiella bacteria, with the highest potential as bioremediation agents. These bacterial isolates were found to enhance the availability of phosphorus in the soil, maintain soil pH, but had no effect on total soil nitrogen. Furthermore, the bacterial isolates exhibited the ability to reduce mercury content after treatment with NA isolates.
PENGUJIAN SEDIAAN SAMPO ANTI KETOMBE DARI EKSTRAK DAUN KETEPENG CINA (Cassia alata L.) DAN SARI BUAHJERUK NIPIS (Citrus aurantifolia S.) SEBAGAI ANTI JAMUR Pityrosporum ovale Siska Aditiani; Rizki Amelia Nasution; Irda Nila Selvia
BIOMA : JURNAL BIOLOGI MAKASSAR Vol. 10 No. 2 (2025): Bioma : Juli - Desember 2025
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University

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Abstract

Dandruff is a scalp condition caused by the fungus Pityrosporum ovale. The use of natural components as active ingredients in anti-dandruff shampoo preparations, such as Chinese longan leaves (Cassia alata L.) and lime (Citrus aurantifolia S.), provides a safer alternative to chemicals that may harm the skin. The purpose of this study is to assess the antifungal activity of a combination of Chinese ketepeng leaf extract (Cassia alata L.) and lime juice (Citrus aurantifolia S.) against Pityrosporum ovale, as well as to determine the optimal concentration of Chinese ketepeng leaf extract and lime juice that inhibits the growth of the Pityrosporum ovale fungus. The research was conducted using an experimental method with a quantitative approach. This study began with testing the activity of lime fruit extracts and juice with concentrations of 10%, 15%, 20%, 25%, and 30%, but obtained the best results for testing shampo preparations based on Pharmacopoeia IV, namely at concentrations of 20% and 25%, and two supporting controls, namely control (-) without extracts and juice and control (+) which is clear shampoo. The study found that the inhibition zone on Pityrosporum ovale fungi was 10.16 mm in the control (-), 16.36 mm at the 20% concentration, 20.10 mm at the 25% concentration, and 20.30 mm in the (+) control. Chinese ketepeng leaf extract and lime juice can work well as an alternative ingredient in natural anti-dandruff shampoo formulations. The results of the preparation evaluation revealed that the formula with a concentration of 20% met the characteristics of a good shampoo according to Pharmacopoeia IV.
Analisis Komparatif Pengaruh Faktor Lingkungan Abiotik dan Biotik terhadap Diversitas Flora Normal Ayam : Sebuah Tinjauan Sistematis Lestari Lestari; Rizki Amelia Nasution
Jurnal Cakrawala Pendidikan dan Biologi Vol. 2 No. 4 (2025): Desember: Jurnal Cakrawala Pendidikan dan Biologi
Publisher : Asosiasi Riset Ilmu Pendidikan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61132/jucapenbi.v2i4.883

Abstract

The normal flora of chickens refers to the microbial communities that naturally inhabit the gastrointestinal tract and body surfaces of chickens, playing a crucial role in health, digestion, and immunity. This community consists of beneficial bacteria such as Lactobacillus, Bifidobacterium, and Bacillus, which assist in feed fermentation, vitamin synthesis, and inhibition of pathogenic growth. Additionally, the normal flora may include potential pathogenic bacteria such as Escherichia coli, Salmonella spp., and Campylobacter, which can cause diseases if microbial balance is disrupted. The diversity and balance of the microbiota are influenced by various abiotic factors, including feed quality, access to clean water, ambient temperature, humidity, and environmental hygiene. Biotic factors, such as microbial interactions, rearing systems, and contact with other animals, also play a significant role in determining microbial composition. Several studies have shown that the use of feed additives, such as probiotics and phytogenics, can enhance populations of beneficial bacteria while suppressing pathogenic bacteria. Extensive rearing systems, which provide chickens with more space and exposure to natural environments, tend to increase microbiota diversity compared to semi-intensive systems with more restricted conditions. Understanding these factors is essential for developing effective health management strategies and optimizing safe and sustainable poultry production.
Identifikasi Bakteri Indigenous Sebagai Agen Bioremediasi di Lahan Pertanian Cabai Merah Keriting (Capsicum Annum) Yang Terpapar Pestisida Profenofos Sarah Safira; Kartika Manalu; Rizki Amelia Nasution
JURNAL RISET RUMPUN MATEMATIKA DAN ILMU PENGETAHUAN ALAM Vol. 5 No. 2 (2026): Agustus : JURRIMIPA: Jurnal Riset Rumpun Matematika dan Ilmu Pengetahuan Alam
Publisher : Lembaga Pengembangan Kinerja Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55606/jurrimipa.v5i2.9838

Abstract

Indigenous bacteria are microorganisms that naturally live and adapt to specific environments, making them unique to that area. In bioremediation, these bacteria play a crucial role due to their ability to degrade various environmental contaminants, such as the pesticide profenofos left in the soil. This ability makes indigenous bacteria effective and cost-efficient agents in addressing environmental pollution, highlighting their importance in agricultural land recovery research. The aim of this study is to isolate and identify indigenous bacteria from soil exposed to profenofos pesticide and analyze them descriptively. The study is divided into several stages: sample collection, isolation using Nitrate Minerals Salts (NMS) medium, ability testing using Muller Hilton Agar (MHA) medium, characterization, Gram staining, and biochemical testing. The results revealed that 8 isolates have potential as bioremediation agents for soil contaminated with profenofos. In the ability test against the pesticide, the antibacterial inhibition of profenofos was categorized into four levels: weak, moderate, strong, and very strong. The results showed that the isolate with the code BIPC 8 had the highest clear zone diameter of 32.95 mm, indicating a very strong ability. Out of the 8 isolates, 7 were categorized as strong and 1 as very strong, showing potential to reduce profenofos residues in the soil. The identified genera of these isolates include Nitrosoccocus, Bacillus, Pseudomonas, and Rhodococcus. In conclusion, indigenous bacteria are capable of degrading profenofos pesticide.
Penurunan Kadar Tembaga (Cu) dengan Biostimulasi Glukosa pada Bakteri Indigenous dari Sungai Bedera Kecamatan Medan Marelan, Kota Medan, Sumatera Utara Resti Ayunda Lubis; Rizki Amelia Nasution; Leni Widiarti
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 7 No 4 (2025): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2025.7.4.18072

Abstract

Sungai Bedera adalah sungai yang tergolong tercemar logam berat akibat pembuangan limbah rumah tangga dan industri yang memiliki kadar logam berat tembaga (Cu) yang tinggi yaitu 0,6518 mg/L. Cara yang dilakukan untuk menurunkan kadar logam berat tembaga (Cu) adalah dengan memanfaatkan bakteri Indigenous. Penelitian ini bertujuan untuk mengidentifikasi bakteri indigenous dari Sungai Bedera dan menguji kemampuannya dalam menurunkan kadar Cu. Bakteri Indigenous diberi perlakuan dengan nutrisi tambahan yaitu biostimulan glukosa. Metode yang dilakukan yaitu isolasi bakteri indigenous, uji resistensi bakteri dengan menghitung nilai Optical Density (OD) menggunakan spektrofotometer Uv-Vis dan proses penurunan kadar logam berat (Cu) dilakukan dengan metode SSA (Spektrofotometer Serapan Atom). Hasil uji menggunakan One Way ANOVA menunjukkan adanya perbedaan nyata antarperlakuan (Sig. < 0,05). Empat genus bakteri indigenous yang berhasil diidentifikasi memiliki kemampuan menurunkan kadar logam berat tembaga (Cu) yaitu Enterococcus, Bacillus, Corynebacterium, dan Staphylococcus. Persentase penurunan Cu tanpa biostimulan glukosa pada masing-masing bakteri Enterococcus, Bacillus, Corynebacterium dan Staphylococcus adalah 51,49%, 40,65%, 29,39%, dan 20,70%. Persentase penurunan Cu dengan penambahan biostimulan glukosa pada masing-masing bakteri Bacillus, Enterococcus, Staphylococcus dan Corynebacterium adalah (77,60%), (72,28%), (66,66%), dan (60,63%). Bakteri indigenous yang paling tinggi dalam menurunkan kadar tembaga (Cu) dengan penambahan biostimulan glukosa adalah Bacillus dengan persentase penurunan 77,60% dan bakteri indigenous yang paling rendah dalam menurunkan kadar tembaga (Cu) tanpa penambahan biostimulan glukosa adalah Staphylococcus dengan persentase penurunan 20,70%. Kata kunci : Bakteri Indigenous, Biostimulan, Logam Berat Tembaga (Cu), dan Sungai Bedera
Co-Authors Irda Nila Selvia Kartika Manalu Leni Widiarti Lestari Lestari Muhammad Idris Resti Ayunda Lubis Revina Yani Tarigan sarah safira Siska Aditiani Ulfayani Mayasari Ulfayani Mayasari Zahratul Idami