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All Journal BIOMA : Jurnal Biologi Makassar Bioma : Jurnal Biologi dan Pembelajaran Biologi Jurnal Teknik dan Science (JTS) Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Rizki Amelia Nasution
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Control & Systems Engineering Electrical & Electronics Engineering Environmental Science Health Professions Industrial & Manufacturing Engineering

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KELIMPAHAN KOLONI BAKTERI Vibrio sp. BERDASARKAN LOKASI BUDIDAYA TAMBAK UDANG DI KABUPATEN PIDIE Zahratul Idami; Rizki Amelia Nasution
BIOMA Vol 5, No 2 (2020): BIOMA:JURNAL BIOLOGI DAN PEMBELAJARAN BIOLOGI
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32528/bioma.v5i2.4012

Abstract

Penelitian ini bertujuan untuk mengisolasi dan menghitung kelimpahan koloni bakteri Vibrio sp. dari air tambak udang dengan 5 lokasi yang berbeda di Kecamatan Simpang Kabupaten Pidie, Aceh. Penelitian ini menggunakan metode eksploratif dan pengambilan sampel menggunakan purposive sampling. Isolasi bakteri menggunakan media selektif TCBSA. Perhitungan kelimpahan koloni Vibrio sp. menggunakan metode Total Plate Count. Hasil penelitian menunjukkan bahwa kelimpahan bakteri Vibrio sp. di Kecamatan Kembang Tanjong untuk kelima kategori lokasi tambak tergolong aman (104 cfu/ml) yaitu 6,2 x 102 – 2,6 x 103 cfu/ml. Berbeda halnya dengan tambak di Simpang Tiga yang masih dapat dikatakan aman hanya tambak K2T2 (2,9 x 103 cfu/ml), K4T2 (2,2 x 104 cfu/ml, K2T1; K5T; K5T2 (2,3 x 104 cfu/ml) karena kelimpahannya 8,34 x 104 cfu/ml, sedangkan bakteri Vibrio lainnya sudah bersifat patogen (105 cfu/ml). Hasil pengukuran kualitas air tambak menunjukkan rata-rata nilai salinitas 10,67-30,33 ppt, suhu 27,9-33,8 oC, pH air 7,65-8,45, DO 5,35-8,42 dan kecerahan 20-55 cm. Tidak adanya hubungan korelasi yang signifikan dari kualitas air tambak terhadap kelimpahan koloni bakteri Vibrio sp.
UJI AKTIVITAS MINYAK ATSIRI DAUN EKOR KUCING (Acalypha hispida) SEBAGAI FUNGISIDA ALAMI TERHADAP JAMUR Sclerotium rolfsii DAN Fusarium oxysporum SECARA IN-VITRO Revina Yani Tarigan; Ulfayani Mayasari; Rizki Amelia Nasution
BIOMA : JURNAL BIOLOGI MAKASSAR Vol. 9 No. 1 (2024): Bioma : Januari - Juni 2024
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University

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Abstract

Penyakit busuk batang dan layu Fusarium merupakan penyakit yang disebabkan oleh jamur patogen yaitu Sclerotium rolfsii dan Fusarium oxysporum. Pengendalian dilakukan dengan pemberian fungisida alami minyak atsiri daun ekor kucing yang ramah lingkungan serta mengandung senyawa flavonoid, saponin, dan tannin. Penelitian ini bertujuan untuk mengetahui aktivitas fungisida alami minyak atsiri daun ekor kucing terhadap pertumbuhan jamur Sclerotium rolfsii dan Fusarium oxysporum secara in – vitro. Metode yang digunakan dalam pembuatan minyak atsiri daun ekor kucing menggunakan metode destilasi uap, serta uji pada aktivitas antijamur menggunakan metode difusi cakram dengan konsentrasi sampel dan kontrol positif 2,5%, 5%, 7,5%, dan 10%, untuk pembuatan produk fungisida alami minyak atsiri daun ekor kucing menambahkan larutan Tween 80. Berdasarkan hasil uji menggunakan Analysis of Variance (ANOVA) penelitian ini menunjukan adanya aktivitas antijamur fungisida alami minyak atsiri daun ekor kucing terhadap jamur Sclerotium roflsii dan Fusarium oxysporum dengan nilai signifikan p< 0.05. Hasil fungisida alami minyak atsiri daun ekor kucing menggunakan konsentrasi 2,5%, 5%, 7,5% dan 10% diperoleh hasil diameter zona hambat pada jamur Sclerotium roflsii yaitu 11,23, 7,6, 6,9 dan 6,0. Sedangkan hasil uji diameter zona hambat pada jamur Fusarium oxysporum 18,26, 19,96. 21,53 dan 22,53. Berdasarkan hasil dari rata – rata aktivitas antijamur zona hambat minyak atsiri daun ekor kucing dapat dijadikan sebagai fungisida alami terhadap jamur Sclerotium roflsii pada konsentrasi efektif 2,5%. Sedangkan jamur Fusarium oxysporum pada konsentrasi 2,5%. Kata Kunci: Minyak atsiri, Fungisida alami, Sclerotium roflsii dan Fusarium oxysporum Kata kunci : Minyak atsiri, Fungisida Alami, Sclerotium roflsii, Fusarium oxysporum
ISOLATION OF BACTERIA AS A BIOREMEDIATION AGENT FOR RECLAMATION OF MERCURY-CONTAMINATED SOILS Muhammad Idris; Rizki Amelia Nasution; Ulfayani Mayasari
Jurnal Bioteknologi dan Biosains Indonesia Vol. 10 No. 1 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

Gold mining commonly involves the use of mercury, which generates hazardous and toxic mercury-based waste, leading to soil contamination and adverse effects on human health. Bioremediation has been explored as a potential solution to this issue. This study aimed to identify indigenous microbial species with potential as bioremediation agents, assess their ability to influence chemical properties, and evaluate their effectiveness in reducing mercury content. The research was conducted in five stages: isolation of native bacteria, testing the potential of microorganisms, assessing the ability to reduce mercury, conducting soil tests, and identifying bacterial characteristics through microscopic and biochemical analyses. The results revealed three types of indigenous microbes, namely Pseudomonas, Neisseria, and Klebsiella bacteria, with the highest potential as bioremediation agents. These bacterial isolates were found to enhance the availability of phosphorus in the soil, maintain soil pH, but had no effect on total soil nitrogen. Furthermore, the bacterial isolates exhibited the ability to reduce mercury content after treatment with NA isolates.
PEMBUATAN MEDIA PERTUMBUHAN BAKTERI ESCHERICHIA COLI DENGAN MENGGUNAKAN UMBI UBI JALAR PUTIH IPOMOEA BATATAS Ita Hasanah Br Karo; Rasyidah, Rasyidah; Rizki Amelia Nasution
Jurnal Teknik dan Science Vol. 3 No. 3 (2024): Oktober: Jurnal Teknik dan Science
Publisher : Asosiasi Dosen Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56127/jts.v3i3.1894

Abstract

Nutrient Agar (NA) is a growth medium for heterotrophic microorganisms made from meat extract, peptone and agar, but commercially it is relatively expensive. As an alternative, researchers innovated using white sweet potatoes (Ipomoea batatas) which are rich in carbohydrates, protein, fat and natural fiber as a growth medium for Escherichia coli bacteria. The aim of this research is to examine the use of white sweet potato flour as a growth medium for Escherichia coli bacteria, and compare it with a medium that is added with meat broth. This research used the experimental research method twice with three repetitions of sweet potato flour, namely 10gr (w/v), 15gr (w/v), 20gr (w/v) and NA control. After conducting research on white sweet potato flour, it was found that the water content test results met the standards of around 8.56–9.66%. The panelists' organoleptic test stated that white sweet potato flour had a smooth texture, yellowish white color, slightly sweet aroma. The solubility test proved that white sweet potato flour does not dissolve in water, and the pH test showed a value of 6.8, the percentage of carbohydrates contained was 74.32%, and protein 1.36%. The results obtained by the number of bacterial colonies with the best treatment without broth were F3 (20 gr white sweet potato flour) 25.5 x 106 CFU and the best treatment with the addition of broth was F2 (sweet potato flour + 15 gr broth) 28.7 x 106 while the NA control showed results of 25.6 x 106 CFU. It can be concluded that white sweet potato flour meets all test standards so that it can be used as an alternative growth medium for bacteria, especially Escherichia coli.
PENGUJIAN SEDIAAN SAMPO ANTI KETOMBE DARI EKSTRAK DAUN KETEPENG CINA (Cassia alata L.) DAN SARI BUAHJERUK NIPIS (Citrus aurantifolia S.) SEBAGAI ANTI JAMUR Pityrosporum ovale Siska Aditiani; Rizki Amelia Nasution; Irda Nila Selvia
BIOMA : JURNAL BIOLOGI MAKASSAR Vol. 10 No. 2 (2025): Bioma : Juli - Desember 2025
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University

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Abstract

Dandruff is a scalp condition caused by the fungus Pityrosporum ovale. The use of natural components as active ingredients in anti-dandruff shampoo preparations, such as Chinese longan leaves (Cassia alata L.) and lime (Citrus aurantifolia S.), provides a safer alternative to chemicals that may harm the skin. The purpose of this study is to assess the antifungal activity of a combination of Chinese ketepeng leaf extract (Cassia alata L.) and lime juice (Citrus aurantifolia S.) against Pityrosporum ovale, as well as to determine the optimal concentration of Chinese ketepeng leaf extract and lime juice that inhibits the growth of the Pityrosporum ovale fungus. The research was conducted using an experimental method with a quantitative approach. This study began with testing the activity of lime fruit extracts and juice with concentrations of 10%, 15%, 20%, 25%, and 30%, but obtained the best results for testing shampo preparations based on Pharmacopoeia IV, namely at concentrations of 20% and 25%, and two supporting controls, namely control (-) without extracts and juice and control (+) which is clear shampoo. The study found that the inhibition zone on Pityrosporum ovale fungi was 10.16 mm in the control (-), 16.36 mm at the 20% concentration, 20.10 mm at the 25% concentration, and 20.30 mm in the (+) control. Chinese ketepeng leaf extract and lime juice can work well as an alternative ingredient in natural anti-dandruff shampoo formulations. The results of the preparation evaluation revealed that the formula with a concentration of 20% met the characteristics of a good shampoo according to Pharmacopoeia IV.
Co-Authors Irda Nila Selvia Ita Hasanah Br Karo Muhammad Idris Rasyidah Rasyidah Revina Yani Tarigan Siska Aditiani Ulfayani Mayasari Ulfayani Mayasari Zahratul Idami