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Isolasi Mikroorganisme Anaerob Limbah Cair Tekstil Menggunakan Desikator Sebagai Inkubator Anaerobik Dianty Rosirda Dewi Kurnia; Ira Permatasari; Yuni Rafika
Fluida Vol 11 No 1 (2015): FLUIDA
Publisher : Jurusan Teknik Kimia, Politeknik Negeri Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35313/fluida.v11i1.554

Abstract

Isolasi Mikroorganisme Anaerob Limbah Cair Tekstil Menggunakan Desikator Sebagai Inkubator Anaerobik. Penggunaan mikroorganisme untuk mengolah limbah cair tekstil yang mengandung bahan organik tinggi sangat potensial untuk dikembangkan. Mikroorganisme anaerob dapat digunakan pada pengolahan limbah cair, yaitu untuk mendegradasi senyawa- senyawa organik kompleks berantai panjang menjadi senyawa yang lebih sederhana sehingga dapat menurunkan beban kerja dari pengolahan aerobik. Penelitian ini bertujuan untuk mengetahui efektivitas desikator termodifikasi sebagai inkubator anaerob dan melakukan isolasi mikroba dari unit pengolahan air limbah tekstil sehingga diperoleh konsorsium mikroorganisme (mixed culture) anaerobik. Desikator dimodifikasi dengan dialiri gas nitrogen untuk menghilangkan gas oksigen di dalam desikator. Parameter yang digunakan sebagai indikator adalah pengamatan secara makroskopis, mikroskopis dan uji pembentukan hidrogen sulfida. Isolat pembanding yang digunakan adalah Pseudomonas aeruginosa sebagai bakteri aerob obligat, Escherichia coli sebagai bakteri anaerob fakultatif, Desulvofibrio desulfuricans dan Methanobrevibacter ruminantium sebagai anaerob obligat. Desikator termodifikasi juga digunakan sebagai inkubator untuk melakukan isolasi. Hasil penelitian menunjukkan bahwa desikator yang dimodifikasi mempunyai efektivitas sebagai inkubator anaerob meskipun bakteri obligat aerob dan aerob fakultatif masih dapat tumbuh karena pada media yang digunakan masih terdapat oksigen terlarut yang ditandai dengan warna pink di bagian atas media dan lilin dinyalakan di dalam desikator dapat juga digunakan sebagai indikator keberadaan oksigen. Desikator ini juga menunjukkan efektivitas untuk digunakan sebagai inkubator anaerob. Pada proses isolasi dari pengolahan limbah anaerob pabrik tekstil diperoleh 6 jenis isolat yang dapat digunakan sebagai konsorsium mikroorganisme (mixed culture) anaerobik.
Pembuatan Edible Film Antibakteri Berbahan Dasar Pektin Albedo Semangka, Sagu, dan Ekstrak Bawang Putih Fitria Yulistiani; Dianty Rosirda Dewi Kurnia; Miranti Agustina; Yaumi Istiqlaliyah
Fluida Vol 12 No 1 (2019): FLUIDA
Publisher : Jurusan Teknik Kimia, Politeknik Negeri Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35313/fluida.v12i1.1621

Abstract

Edible film berbahan dasar pati memiliki kelemahan pada sifat kuat tarik, elastisitas, dan kemampuan antimikroba. Sifat-sifat tersebut dapat ditingkatkan dengan menambahkan pektin, pemlastis, dan aditif. Penelitian ini dilakukan untuk mengkaji pembuatan edible film dari pektin albedo semangka dengan penambahan sorbitol dan gliserol sebagai bahan pemlastis, tepung sagu sebagai tambahan sumber pektin, dan ekstrak bawang putih sebagai aditif antimikroba. Variasi yang dilakukan adalah penambahan ekstrak bawang putih (0% ; 2,5% ; 5% ; 7,5% ; 10%) dan jenis pemlastis (gliserol dan sorbitol). Karakteristik yang diamati meliputi kuat tarik, perpanjangan, dan zona hambat bakteri. Edible film dengan karakteristik terbaik yaitu kuat tarik 366,4 kgf/cm2, perpanjangan 35,4%, dan zona hambat bakteri 16,5 mm (metode sumuran) adalah sampel dengan konsentrasi ekstrak bawang putih 10% dan pemlastis gliserol.
Adsorption of Methylene Blue Using Activated Carbon Bioadsorbent Coffee Grounds and Reed Grass Without Flow Rate Hidayatulloh, Irwan; Kurnia, Dianty Rosirda Dewi; Anggorowati, Heni; Muhari, Emma Hermawati; Kharisma, Luthfiana; Sari, Radianti Novita; Paramitha, Tifa; Sihombing, Rony Pasonang
Fluida Vol. 17 No. 2 (2024): FLUIDA
Publisher : Department of Chemical Engineering, Politeknik Negeri Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35313/fluida.v17i2.6269

Abstract

Methylene blue adsorption research has been conducted using bioadsorbents from activated carbon of coffee grounds and reeds. Coffee grounds and reeds contain carbon and cellulose that are effective for binding dyes. This study aims to determine the characteristics of activated carbon from coffee grounds and reeds based on SNI-06-3730-1995 and assess the color reduction of wastewater referring to the Decree of the Minister of Environment No. 51/MENLH/1995, which sets the maximum methylene blue content in industrial wastewater at 5-10 mg/L. The research method includes bioadsorbent preparation, characterization, and adsorption process with UV-Vis spectrophotometric analysis. Mass ratio variation was conducted on each adsorbent at each contact time variation and methylene blue concentration variation. The results of the analysis showed that the activated carbon of coffee grounds and reeds fulfills SNI-06-3730-1995 which is characterized by values that are below the maximum value in each parameter. The effectiveness of methylene blue adsorption by coffee grounds and reeds activated carbon reached 98.15% at a ratio of coffee grounds and reeds activated carbon 0:1 (w/w) for 120 minutes at a concentration of 25 mg/L. The adsorption capacity using the Freundlich isotherm method on reed activated carbon was 1.100 mg/g and coffee grounds activated carbon was 1.019 mg/g. The adsorption process of coffee grounds and reeds activated carbon has no effect on the carbon and cellulose structure of coffee grounds and reeds contained in activated carbon, namely O-H, C-H, C=C, and C=O.
Phosphoric Acid-Activated Carbon From Coffee Grounds and Fly Ash For Efficient Laundry Wastewater Adsorption Muhari, Emma Hermawati Muhari; Silalahi, Rafael Leonardo; Puspita, Nina; Maryani, Anisya Sri; Sihombing, Rony Pasonang; Adhitasari, Alfiana; Kurnia, Dianty Rosirda Dewi
Journal of Green Science and Technology Vol 9 No 2 (2025): Journal of Green Science and Technology Vol. 9 No.2 September 2025
Publisher : Faculty of Engineering, Universitas Swadaya Gunung Jati

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33603/jgst.v9i2.10649

Abstract

Laundry waste that is directly discharged into water bodies causes pollution due to its chemical content, such as phosphate. The adsorption method is chosen as an option to reduce the phosphate content in laundry waste due to its simple preparation and low cost. The adsorbent used is a mixture of coffee grounds and fly ash. The use of coffee grounds is based on the increasing waste from coffee shops, thus being utilized as an adsorbent. The aim of this study is to determine the effectiveness of coffee grounds and fly ash with the best combination of composition and contact time using the adsorption method. The stages begin with the preparation of coffee grounds and fly ash through carbonization and activation using H3PO4. The variations performed consisted of variations in the composition of coffee grounds and fly ash with a total of 1 gram in ratios of 1:4; 2:3; 1:1; 3:2; 4:1 and contact time variations of 15 minutes, 30 minutes, 45 minutes, 60 minutes, and 75 minutes. Based on the research results, the highest percentage of removal was found in the composition variation of coffee grounds and fly ash with a ratio of 1:4 for 75 minutes.
Characterization of Coffee Business Waste in the form of Robusta Coffee Dregs as Adsorbent: A Preliminary Study Sihombing, Rony; Soeswanto, Bambang; Adhitasari, Alfiana; Yusuf, Yusmardhany; Putri, Alifhah Ananda; Fatah, Khalaida Fania; Clarisa, Shintiya; Gunawan, Sinna Chaerunnabila; Leoanggraini, Unung; Muhari, Emma Hermawati; Kurnia, Dianty Rosirda Dewi
Journal of Green Science and Technology Vol 9 No 1 (2025): Journal of Green Science and Technology Vol. 9 No. 1 March 2025
Publisher : Faculty of Engineering, Universitas Swadaya Gunung Jati

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33603/jgst.v9i1.9104

Abstract

Robusta coffee grounds are widely produced in several types of businesses, one of which is the coffee industry (coffee shop). This pulp can be reused by business people by being used as an adsorbent and paired with the industrial waste. The purpose of this research is to identify the carbon of Robusta coffee grounds between before and after chemical activation. The methods used were sieving, drying at 25°C to 110°C, carbonization at 600°C, immersion in 0.1M HCl solution. Carbonization of Robusta coffee grounds was carried out using BET, FTIR and SEM. The results showed an increase in carbon surface area from 0.588 m2/g to 14.609 m2/g. The results of the FTIR method showed a change in functional groups on several peaks between pre- and post-activation conditions. The results of the SEM method showed an increase in pore size from 5.5 µm (before activation) to 11 µm (after activation). Keywords: Robusta coffee, Coffee grounds, FTIR, Adsorbent