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All Journal Reaktor Bioma : Berkala Ilmiah Biologi Jurnal Natur Indonesia Biopropal Industri JURNAL BIOLOGI INDONESIA Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology ANNALES BOGORIENSES Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Teknologi Indonesia
Ahmad Thontowi
Research Center for Biotechnology-LIPI
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Education Energy Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology

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Optimization of Xylose Production from Sugarcane Trash by Microwave-Maleic Acid Hydrolysis Hermiati, Euis; Oktaviani, Maulida; Ermawar, Riksfardini Annisa; Laksana, Raden Permana Budi; Kholida, Lutfi Nia; Thontowi, Ahmad; Mardiana, Siti; Watanabe, Takashi
Reaktor Volume 20 No.2 June 2020
Publisher : Dept. of Chemical Engineering, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (528.661 KB) | DOI: 10.14710/reaktor.20.2.81-88

Abstract

Sugarcane trash contains significant amount of xylan that could be hydrolysed to xylose. The xylose could be further fermented to produce xylitol, a sugar alcohol that has low calories and does not cause carries of teeth. In this study we optimized the production of xylose from sugarcane trash by microwave-assisted maleic acid hydrolysis using response surface methodology (RSM). The factors optimized were acid concentration, time, and temperature. The xylose yield based on the weight of initial biomass was determined and it served as a response variable. Results show that acid concentration and interaction between time and temperature had significant effect on xylose yield. The quadratic regression model generated from the optimization was fit and can be used to predict the xylose yield after hydrolysis with various combinations of acid concentration, time, and temperature. The optimum condition for xylose production from sugarcane trash was using maleic acid of 1.52%, and heating at 176 °C for 6.8 min. At this condition the yield of xylose was 24.3% per initial biomass or 0.243 g/ g biomass.Keywords: maleic acid; microwave heating; response surface methodology; sugarcane trash, xylose
Produksi Xilitol Menggunakan Hidrolisat Tongkol Jagung (Zea mays) Oleh Meyerozyma caribbica InaCC Y67 Faradila Ayu, Near Putri; Nurhayati, N; Thontowi, Ahmad; Kusdiyantini, Endang; Kanti, Atit; Hermiati, Euis
Bioma : Berkala Ilmiah Biologi Vol. 23, No 1, Tahun 2021
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.23.1.71-77

Abstract

Xylitol (C5H12O5) is a non-carcinogenic polyalcoholic sugar. Xylitol is beneficial for diabetics because it can be metabolized without insulin. Corn cobs contain 30% xylose which can be fermented into xylitol by microorganisms. Xylitol can be produced by fermentation of xylose and few microorganisms. Meyerozyma caribbica is a yeast that has been proven to produce xylitol and inhibitor’s resistant. The aim of this research is to test the xylitol productivity by Meyerozyma caribbica InaCC Y67 using corn cobs hydrolyzate and the effect of the volume of fermentation media on xylitol productivity by Meyerozyma caribbica InaCC Y67. The method was carried out by culturing Meyerozyma caribbica InaCC Y67 as a starter on YPD media.  Fermentation using 100 mL Erlenmeyer with the variation of fermentation volume is 10 ml and 75 ml, agitation 175 rpm and 30 oC. Parameters were measured based on the dry weight of cells, xylose and xylitol. Data were analyzed using fermentation kinetics. The results of analysis showed that the higher xylitol production was found in the fermentation volume 75 ml with an efficiency value of 7,171%. The highest xylitol production was at the 48th hour with production value of 2.050 g/L. Results from research shows that Meyerozyma caribbica InaCC Y67 can produce xylitol with corn cobs hydrolyzate. The right volume of fermentation in the fermentation process can also increase the productivity of xylitol.
Co-Authors - Yopi A.A. Ketut Agung Cahyawan W ADE ANDRIANI Atit Kanti BAMBANG SUNARKO Elvi Yetti, Elvi Endang Kusdiyantini Euis HERMIATI Faradila Ayu, Near Putri Hadi, Sofjan Hadi, Sofjan Hapzi Ali Kholida, Lutfi Nia Kusmiati Kusmiati Laksana, Raden Permana Budi Maemonah, Maemonah N Nurhayati NANIK RAHMANI Ni Nyoman Tri Puspaningsih Ni'mahtuzahroh, Ni'mahtuzahroh NUNIK SULISTINAH Nuzul Farini, Nuzul Oktaviani, Maulida Purkan Purkan, Purkan Purwoko, Agus Putra, Filemon Jalu Nusantara Riksfardini Annisa ERMAWAR Safitriani, Safitriani Safitriani, Safitriani Siti Mardiana Sukma Nuswantara Suryani Suryani Susiana Purwantisari Takashi Watanabe Tanjung, Puspasari Noerwan Tanjung, Puspasari Noerwan Wijaya, Hans YOPI YOPI