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All Journal Jurnal Natur Indonesia Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Buletin Kebun Raya REINWARDTIA BERITA BIOLOGI JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Journal of Tropical Crop Science Teknologi Indonesia Berita Biologi
Eka Fatmawati Tihurua
Botany Division, Research Center for Biology, Indonesian Institute of Sciences Jl. Raya Jakarta-Bogor Km 46 Cibinong 16911
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Education

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Journal : Journal of Tropical Crop Science

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Histological Analysis of Somatic Embryogenesis from Immature Zygotic Embryo of Wild Banana Musa acuminata ssp. malaccensis Handayani, Tri; Martanti, Diyah; Prawestri, Apriliana Dyah; Tihurua, Eka Fatmawati; Maharijaya, Awang; Wahyu, Yudiwanti; Sobir; Witjaksono
Journal of Tropical Crop Science Vol. 11 No. 03 (2024): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.11.03.307-319

Abstract

Somatic embryogenesis, a crucial plant regeneration method, has become indispensable for crop improvement, particularly for species reliant on somatic cell manipulation techniques. Optimization of this process necessitates an understanding of the developmental stages involved. This study investigates the histological aspects of somatic embryogenesis in Musa acuminata ssp. malaccensis derived from immature zygotic embryos. Through detailed histological analysis, we aimed to elucidate the morphological changes and cellular organization occurring during the various stages of somatic embryogenesis, from induction, culture proliferation, and somatic embryo development to plantlet conversion. The initial stages of embryogenesis, characterized by nodules, were primarily composed of meristematic cells with high cell division activity. These cells contained tetrad-like structures that could develop into distinct two- and four-celled proembryoids or proembryogenic aggregates. Our histo-anatomical analysis revealed that embryogenic cultures proliferated through multiple pathways simultaneously: somatic embryo budding, proembryo formation, and pro-embryonic mass formation from both internal and peripheral cells. At the stage of somatic embryo development, embryos with a well-defined protoderm layer, containing cells with prominent nuclei and dense cytoplasm, potentially regenerate into plantlets. Furthermore, histological examination revealed the presence of procambium within mature somatic embryos, which subsequently developed into the vascular system of the complete plantlet
Co-Authors Agustiani, Esthi Liani Apandi , Ismail Ardhiyani , Marlina Asmar Hasan Awang Maharijaya Botek, Muhammad Dalimunthe, Syadwina H. Decky Indrawan Junaedi Deden Girmansyah, Deden Diah Sulistiarini Diyah Martanti, Diyah Erlin Rachman Firihu, Muhammad Zamrun GUSNAWATY HS, GUSNAWATY Hutabarat, Prima W.K. I PUTU GEDE P. DAMAYANTO, I PUTU GEDE P. Ina Erlinawati Inggit Puji Astuti MARTIANSYAH, Irfan MUHAMMAD TAUFIK Nasution, Taufikurrahman Prawestri, Apriliana Dyah Rahmawati, Kusuma Rugayah Rugayah Siti Sunarti Sobir Sobir Sulistyawati, Endah Sunaryo Sunaryo Sunaryo,, Sunaryo Surya, Dede Sutikno Sutikno Tahan Uji Teguh Triono, Teguh Tri Handayani Viska Inda Variani WIDOYANTI, WIDOYANTI Witjaksono Wulansari, Tri Yuni Indah Yessi Santika, Yessi Yudiwanti Wahyu E. Kusumo