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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Fitopatologi Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology
Nurita Toruan-Mathius
Plant Production and Biotechnology Div., TCLZ and Biotechnologi Lab., PT SMART Tbk, Jl. Raya Pajajaran 78 F-6, Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Immunology & microbiology Medicine & Pharmacology Veterinary

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Journal : Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)

 1 
Karakterisasi Morfologi Bunga dan Buah Abnormal Kelapa Sawit (Elaeis guineensis Jacq) Hasil Kultur Jaringan Hetharie, Helen; Wattimena, Gustav A.; Thenawidjaya S., Maggy; Aswidinnoor, Hajrial; Toruan-Mathius, Nurita; Ginting, Gale
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 35 No. 1 (2007): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (665.766 KB) | DOI: 10.24831/jai.v35i1.1310

Abstract

Tissue culture-derived plants of oil palm (Elaeis guineensis Jacq) can produce abnormal female dan male flowers, especially female, which develop into mantled fruit. This abnormality have caused yield lost up to 40%. The objectives of this research were to study the stages of flower development in which the abnormality started to appear, to characterize the morphology of the abnormal flower and fruit, and to determine the degree of abnormality. Plant materials used in this research were MK152, MK 176, MK 203, MK163, MK104, MK 212 and MK 209 clones obtained from the collection of "Badan Pengkajian dan Penerapan Teknologi (BPPT)" in Ciampea, Bogor. Characterization of abnormality at the flowering stage was conducted  based on morphological performance at several stages of flower development, while the degree of abnormality at fruiting stage was determined based on the condition of carpel, mesocarp and seed. Normal flower and fruit of the same clone were used as the control. The results showed that the abnormality started at primordial stage of flower organ formation. Supplement carpel was found surrounding gynoecium for the abnormal female flower, which was composed of three to six carpels present at  separate whorl of flower. Abnormal male  flower had no additional whorl of  flower, instead stamens were converted into carpel-like structures. In this case, number of carpels structure depends on the degree of abnormality. There were three levels of abnormality, i.e. light abnormality (AbR), heavy abnormality (AbB) and severe abnormality (AbSB). The severe abnormal fruit was seedless, with soft mesocarp (AbSB1) and woody mesocarp (AbSB2).   Key words : oil palm, mantled fruit, supplement carpel, flower whorl, tissue culture
Keragaman Genetik Kelapa Sawit (Elaeis guineensis Jacq.) Asal Angola Menggunakan Marka SSR Sayekti, Urip; Widyastuti, Utut; Toruan-Mathius, Nurita
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 43 No. 2 (2015): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (479.461 KB) | DOI: 10.24831/jai.v43i2.10420

Abstract

ABSTRACTEffort to increase productivity and other elite characters in Indonesia oil palm breeding program is facing a problem because of the narrow genetic diversity. To broaden the genetic diversity, germplasm exploration has been done in Angola, Central Africa. The objective of this research was to assess the genetic diversity and population structure of Angola originated oil palm germplasm based on 20 SSR markers. The plant materials used were 27 accessions consisted of 136 palms planted in Riau, Sumatera. The DNA was isolated and amplified using PCR. Phylogeny analysis was constructed using Unrooted Neighbor-Joining by DARwin software 6.0.8. The result showed that polymorphic information content (PIC) value is 0.55 (0.17 to 0.75 for each locus) with 102 total number of alleles. Genetic diversity between individuals was higher compared to the genetic diversity within accessions or regions and between accessions or regions. Phylogenetic analysis of 27 accessions showed that accessions were divided into three main groups. Every group containing individuals originated from 5 spatial distribution regions. Principal coordinates analysis (PCoA) showed that accessions were distributed in one structure. Using more primers and samples to get more representative data is recommended for the following research.Keywords: allele, locus, germplasm, molecular marker, polymorphic
Identification of Gene Related to Hard Bunch Phenotype in Oil Palm (Elaeis guineensis Jacq.) ,, Roberdi; ,, Sobir; Yahya, Sudirman; Toruan-Mathius, Nurita; Liwang, Tony
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 43 No. 2 (2015): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (514.814 KB) | DOI: 10.24831/jai.v43i2.10421

Abstract

ABSTRACTMolecular genetic analysis of hard bunch phenomenon in oil palm was done in order to elucidate the role of genetic factor underlying hard bunch in oil palm plantation. The aim of this study was to identify the AFLP primer combination that co-segregates with hard bunch phenotype related gene in oil palm. Molecular analysis was done by bulk segregant analysis approach. DNA was isolated from leaves of the normal and hard bunch palm. DNA from ten individual palms from each category were pooled and used as a template. A total of 56 AFLP primer combinations were selected for selection of polymorphic primer, and as a result it was found that 22 AFLP primer combinations (39.28%) were polymorphic. A total of 48 individual of palm DNA containing 24 individual for each group were further genotyped by those 22 polymorphic markers. Of these, one AFLP primer combination (E-ACC/M-CTG) was obtained as a co-segregated marker that distinguished the hard bunch DNA from the normal one. Based on the analysis of the target sequence aligned to the oil palm DNA sequences available in database, we found that our sequence has similarity with Ty-1 copia retrotransposon. This sequence distribute in all 16 linkage group of oil palm genome.Keywords: abnormal fruits, AFLP, oil palm, Ty-1 copia retrotransposon
Co-Authors ANIDAH DEWI RAHMAWATI Gale Ginting Gustav A. Wattimena Hagia Sophia Khairani HAJRIAL ASWIDINNOOR Helen Hetharie Maggy Thenawidjaya S. Meity Suradji Sinaga Nawangsih, Abjad Asih Roberdi ,, Roberdi Sinthya E, Hana Christine Sobir Sobir Sudirman Yahya Tony Liwang Urip Sayekti UTUT WIDYASTUTI