Bambang Prasetya
Research Center for Biotechnology, LIPI, Cibinong, Bogor 16911

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Isolation and Fermentation of Lactobacillus plantarum JR64 as an Omega 6 Probiotic Producer Lanjar Sumarno; Djumali Mangunwidjaja; Anas M. Fauzi; Khaswar Syamsu; Nastiti Siswi Indrasti; Bambang Prasetya
International Journal of Science and Engineering Vol 2, No 2 (2011)
Publisher : Chemical Engineering Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (596.963 KB) | DOI: 10.12777/ijse.2.2.42-47

Abstract

Application of lactic acid probiotic bacteria in health food diversification currently is progressing rapidly.  It is encouraged the study of searching the potential strains from local resources (Ponorogo Residence) namely noni fruits (badeg pace) and noni wine.  Aims of this study were to perform the isolation, identification, and production of  probiotic Lactobacillus sp. JR64 fermentation process as a producer of Omega-6 (ω-6) lowering cholesterol and design of probiotic creamy product.   Beginning stages of research was strains isolating and in-vitro testing, the best result were used in  molecular  identification technology development for the production of metabolites through the manipulation of environmental variation  of glucose 20 g / l, 30 g / l and 40 g / l that influenced the substrate concentration of linoleic acid productivity. The result of new isolates isolation showed that isolates that obtained from noni wine, Lactobacillus plantarum JR64, was potential as probiotic condidate.   The effieciency of fermentation  substrates using  Yx/s and Yp /s  in the exponential phase was the highest value for the fermentation of 24 hours of  Yx/ s; 17.03% and Y p/s; 74.72%, while the results of design and the best formulation for viability cells of lactobacillus  plantarum probiotics JR64 was composed of 15 g and 50g butter 15 g icing sugar as well as during storage of the refrigerant temperature was 8.92 x 108 CFU / ml.  [Key Words : Omega-6,  Lactobacillus plantarum JR64, fermentation]
Lime Pretreatment on Jabon Wood to Improve Its Reducing Sugar Yield Yusup Amin; Wasrin Syafii; Nyoman J Wistara; Bambang Prasetya
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 12, No 2 (2014): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (517.519 KB) | DOI: 10.51850/jitkt.v12i2.75

Abstract

The purpose of this study was to determine the performance of enzymatic hydrolysis on lime pretreated jabon wood (Anthocephalus cadamba Miq). Proceeding hydrolysis process, the morphology and crystallinity changes of pretreated jabon were also examined. In the present study, jabon wood meal was put into digester and then lime was loaded at varied lime loading, i.e. at 0.1, 0.3, and 0.5 g Ca(OH)2 g-1 of dry biomass in 90 ml of water. The slurry was then homogenized and heated in the oil bath at 100, 125 and 150 C for 2, 4 and 6 hours. Enzymatic hydrolysis was performed in accordance to the NREL procedures using a cellulase enzyme. The enzyme loading variation was 10, 20 and 40 FPU g-1 of the substrate. the RSY of pretreated jabon increased up to 9.7 folds from that of its control. The highest RSY was obtained for pretreatment with 0.5 g Ca(OH)2 g-1 dried biomass at 150 °C for 4 hours and hydrolyzed with 40 FPU of cellulase (118 mg equivalent glucose g-1 dry biomass). This yield was comparable with 14.4% More than a third of the lignin and hemicellulose content (45.83 and 65.52%) were degraded by lime pretreatment.Keywords: enzymatic hydrolysis, jabon wood, lime pretreatment, reducing sugar yield
Pengaruh Persentase Katalis pada Pembuatan Perekat Kayu Cair terhadap Daya Rekatnya sebagai Bahan Perekat Substitusi Fenol Formaldehida pada Pembuatan Kayu Lapis The Effects of Catalyst Percentage Used in Producing Wood Liquid on Its Bond Strength as Phenol Formaldehyde Substitute Adhesive in Plywood Production Bambang Prasetya; Euis Hermiati; Sudijono Sudijono
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 2, No 2 (2004): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (148.102 KB) | DOI: 10.51850/jitkt.v2i2.310

Abstract

The aim of this research was to study the effects of percentage of catalyst used in producing wood liquid (WL) on its bond strength as Phenol Formaldehyde (PF) substitute adhesive in plywood production.  Albizia (Paraserianthes falcataria) wood flour passed 60 mesh sieves having around 5% moisture content was liquefied in phenol solution.  Catalyst used, sulfuric acid 98%, was added at the level of 1%,  2% and 3% of the total phenol solution.  The wood liquid obtained was observed and measured for its physico-chemical properties, including color, total solid, pH, specific gravity and viscosity.  The degrees of PF substitution tried were 100/0, 80/20, 60/40, 40/60 and 20/80 (PF/WL).  Those adhesive mixtures were used in the preparation of plywood made of Meranti (Shorea sp.) and Keruing (Dipterocarpus spp.).  Bond strength of the plywood samples were determined based on the Indonesian National Standard (SNI 01 – 2704 – 1992).  Results of the research showed that Albizia wood flour could be completely liquefied by phenol and sulfuric acid.  Bond strength testing of exterior grade plywood samples of Meranti showed that a quite high percentage of catalyst (3%) decreased bond strength, while that of Keruing showed that the higher the percentage of catalyst, the higher the bond strength.  In general, adhesive composition of 80/20, 60/40 and some 40/60 (PF/WL) can be used as plywood adhesive and meet the SNI for plywood.
PEMANFAATAN LANGSUNG SERBUK KULIT KAYU AKASIA SEBAGAI PEREKAT PAPAN PARTIKEL Direct Utilization of Acacia Bark Powder as Adhesive for Particleboard Subyakto Subyakto; Bambang prasetya
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 1, No 1 (2003): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (175.947 KB) | DOI: 10.51850/jitkt.v1i1.326

Abstract

Many studies have been done on the utilization of tannin from acacia bark obtained through extraction process for wood composites adhesive. In this experiment, powder of acacia bark was used directly for particleboard adhesive without extraction process to save cost and production-processing time.  Acacia (Acacia mangium) bark was powdered into 60 mesh and mixed with wood particles of sengon (Paraserianthes falcataria) with size of 15 mesh. The compositions of wood particles: bark was varied as follows 50:50, 60:40, 70:30, 80:20. Water and paraformaldehyde were added to the mixture. The matt was hot pressed at 180ºC at pressure of 10 kg/cm2 for 15 minutes. The panels were tested for their physical and mechanical properties in accordance with JIS A 5908.The results shown that some physical and mechanical properties of the board with composition of 60 : 40 were met the JIS A 5908 standard. Further studies are needed to improve all the particleboard properties to meet the standard
Pemanfaatan Lumpur Minyak untuk Pembuatan Komposit Berserat Lignoselulosa Utilization of Oil Sludge as a Lignocellulosic Fiber Reinforced Composite Material Bambang Prasetya; Sudijono Sudijono; Purwadi Kasinoputro
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 4, No 1 (2006): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (188.814 KB) | DOI: 10.51850/jitkt.v4i1.283

Abstract

Utilization of Oil Sludge (OS) is facing until now many problems due to heavy metal content. This experiment tried to find out an alternative how to use of OS as a lignocellulosic fiber reinforced composite material. One important reason for this purpose is the wide utilization of composite material for building material. Two problems have to be solved are how to catch the heavy metal and how to increase the bonding ability of OS. In this experiment additive PTA08 was used for catching the heavy metal. The OS with additive PTA were mixed at temperature of 80ºC and fiber in varying composition (40, 50 and 60 % based oven dry weight of raw material). After mat forming, the material was pressed at temperature of 120ºC for 20 minutes. The density of composite was arranged from 0.5, 0.6 and 0.7 g/cm3. Two percent of phenol formaldehyde resin was used to increase the bonding ability of composite material.  The experiment results showed that in general the OS could be used as raw material for production of composite material with medium strength. Modulus of rupture (MOR) could reach 75~120 kg/cm2, while modulus of elasticity (MOE) 5000~7000 kg/cm2. Water absorption of the product was excellent namely below 20 %, and the thickness swelling varied from 8 to 18 %. The leaching test results showed that the leaching of heavy metal was fulfilled the standard
Furfural and 5-(Hydroxymethyl) furfural Tolerance Candida strains in Bioethanol Fermentation Ahmad Thontowi; Urip Perwitasari; Lutfi Nia Kholida; Atit Kanti; Yopi; Bambang Prasetya
Annales Bogorienses Vol. 24 No. 1 (2020): Annales Bogorienses
Publisher : BRIN

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The toxic fermentation inhibitors in lignocellulosic hydrolysates pose significant problems for the production of second-generation biofuels and biochemicals. Among these inhibitors, 5-(hydroxymethyl)furfural (HMF) and furfural are specifically well known. This study investigated the furfural and 5-HMF tolerance in Candida strains, which could be used for the development of advanced generation bioethanol processes. The 10 isolates of Candida were selected based on the cell growth and bioethanol production on YPD medium containing several concentrations of furfural and 5-HMF by using spectrophotometer and HPLC. Candida parapsilosis Y80 could grow and produce bioethanol in the medium that contains furfural and 5-HMF with a concentration of 60 mM and 40 mM, respectively. Based on the results, C. parapsilosis Y80 has potential activity in the development of bioethanol fermentation.