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All Journal HAYATI Journal of Biosciences Prosiding Plantropica: Journal of Agricultural Science agriTECH Jurnal Bioteknologi & Biosains Indonesia (JBBI) Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal BioEksakta : Jurnal Ilmiah Biologi Unsoed
Nurtjahjo Dwi Sasongko
Fakultas Biologi Universitas Jenderal Soedirman, Purwokerto
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Veterinary

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KEKERABATAN MOLEKULER DARI KULTIVAR BUNGA MATAHARI TEDDY BEAR, SKYSCRAPER, LEOM QUEEN DAN BUNGA MATAHARI MENGGUNAKAN MARKA RAPD Tanudharma, Gabriella Ashari; Sasongko, Nurtjahjo Dwi; Yuniaty, Alice
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 1 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.1.1762

Abstract

Helianthus or sunflower is a genus of plant comprising about 70 species. Common sunflower and other members of Helianthae are cultivated in temperate regions and some tropical regions as food crops for humans, cattle, poultry, and as ornamental plants. The common sunflower is valuable with respect of economic and ornamental point of view. There are many cultivars of sunflower including teddy bear, skyscraper, and lemon queen. Variation among these cultivars has been studied using molecular techniques and the result were used to develop the phylogeny among them. Random Amplified Polymorphic DNA (RAPD) is one of molecular techniques that were used for this purpose. The purpose of this study was to contruct the phylogeny of three sunflower cultivars and common sunflower based on RAPD markers. The RAPD primers used in this study were OPA-2, OPA-9, OPA-13, OPB-2, OPB-4, OPB5, OPB-7, and OPB-11. Data analysis based on molecular data showed that genetic relationship among Lemon Queen, Skyscraper, Teddy Bear and Common sunflower based on RAPD markers shows that the cultivars studied are grouped into three main groups, namely: Group I Lemon Queen and Skyscraper, Group II Teddy Bear, and Group III Common sunflower; the closest kinship is shown between Lemon Queen and Skyscraper.
PERTUMBUHAN DAN KANDUNGAN POLIFENOL TANAMAN KALE PADA MEDIA TANAM DENGAN PENAMBAHAN ASAM HUMAT Fadhllurohman, Muhammad Faiq; Proklamasiningsih, Elly; Sasongko, Nurtjahjo Dwi
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 4 No 2 (2022): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2022.4.2.4750

Abstract

Kale (Brassica oleracea) contains some nutritional values like: vitamin C, pro vitamin A (carotenoids), and some compound belong to polyphenol are flavonoids that benefit to its consumer for the growth and health of the body. Polyphenols are bioactive molecules which play an essential role in plants response to ultraviolet irradiation, and high temperature. Flavonoids comprise the most studied group of polyphenols. This group has a common basic structure consisting of two aromatic rings bound together by three carbon atoms that form an oxygenated heterocycle. In order to support growth and increase it’s productivity, cultivation of Kale supposed to be done in good planting media. The addition of organic subtances like humic acid in planting media has proven to increase plant growth and increase productivity in several plants. The objectives of this research were to know the influence of the influence of humic acid on the growth of plant varieties and polyphenol content and to determine the best concentration of humic acid in increasing growth and polyphenol content. The current research method was carried out experimentally a completely randomize design in a split plot method. The main plot was plant’s varieties in 2 levels: V1: Kale Nero toscana (Brassica oleracea var. palmifolia), V2: Siberian dwarf Kale (Brassica oleracea Var. Sabellica). while the sub plot was the HA concentrations in 4 levels i.e 0 g.kg-1, 4 g.kg-1, 8 g.kg-1, and 12 g.kg-1 of planting medium, The parameters used in this study are fresh and dry weight. These data were obtained by weighing the roots and shoots; root’s length, chlorophyll content, and polyphenol content in kale. The data of polyphenol content was obtained by extracting the plant material following a maceration method then calculated using the Folin ciochalteau spectrophotometric. The data of plant’s growth and polyphenol content obtained was analyzed using (F test) with standard deviation rate of 5% and 1%. The results of the analysis of variance which has a significant different, followed by the least significant difference test (LSD). And for the result is the treatment of humic acid increase the growth and polyphenol content of Kale Nero (B. oleracea var. Palmifolia) and Siberian dwarf kale (B. oleracea Var. Sabellica).
Co-Authors Adi Amurwanto AGUS HERY SUSANTO Agus Purwanto AGUS PURWANTO Ahmad Hamim Sadewa Alfik Indarto Alfik Indarto, Alfik Alice Yuniaty Anisa Rohma Dudi Hardianto Dudi Hardianto, Dudi ELLY PROKLAMASININGSIH Elly Proklamasiningsih Fadhllurohman, Muhammad Faiq Gabriella Ashari Tanudharma Hernayanti Hernayanti Hery Winarsi HERY WINARSI Hexa Apriliana Hidayah Indah Nuraeni Nur Fitrianto Rezza Adianti Saefuddin Aziz Siti Samiyarsih SIWI PRAMATAMA MARS WIJAYANTI Sukarti Moeljopawiro Tanudharma, Gabriella Ashari