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Tridjoko Tridjoko
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary

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Pembenihan Selektif Kerapu Bebek, Cromileptes altivelis Turunan ke-2 (F-2) Tridjoko, Tridjoko; Haryanti, Haryanti; Muzaki, Ahmad; Sutarmat, Tatam
Jurnal Perikanan Universitas Gadjah Mada Vol 11, No 2 (2009)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (34.127 KB) | DOI: 10.22146/jfs.4

Abstract

The aim of this experiment was to know the gonad development of humpback grouper Cromileptes altivelis F-2 progeny. The F-2 progeny fishes were produced from selective breeding and composed by Lot 1, Lot 2 and Lot 3. The fishes were reared in the cylinder concrete tanks containing 75 m³, 10 m³ water and floating cages. Fishes were fed with commercial dry pellet in addition with vitamin mix, vitamin C and vitamin E. The result indicated that the mature gonad female of F-2 grouper broodstocks have been achieved. The female broodstocks with 590 g weight produced oocytes with diameter more than 400 µm. Genetic analysis showed that grouper Lot 2 was homogenous, did not find any polymorphism among individual fish, however Lot 1 and Lot 3 still showed high heterozygocity as 0.18 and 0.58, respectively.
Dietary Honeysuckle (Lonicera japonica) Extract Enhances Resistance to Infectious Myonecrosis Virus in Whiteleg Shrimp (Litopenaeus vannamei) Subaidah, Siti; Sumarwan, Joko; Marzuqi, Muhammad; Tridjoko, Tridjoko; Slamet, Bejo; Budi, Darmawan Setia; Pardede, Maria Agustina; Ulkhaq, Mohammad Faizal; Imlani, Ainulyakin; Loh, Jiun-Yan
Jurnal Medik Veteriner Vol. 8 No. 2 (2025): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss2.2025.286-296

Abstract

This study aimed to investigate the immune response of whiteleg shrimp (Litopenaeus vannamei) to prevent infectious myonecrosis virus (IMNV) infection by incorporating Lonicera japonica water extract into artificial feed. A total of 180 whiteleg shrimp were used in four treatment groups (triplicate) receiving different doses of L. japonica extract in their feed, including treatment A (1% w/w), B (2% w/w), C (3% w/w), and D (without supplementation/control). The dietary treatments were applied twice daily for two weeks. On the 15th day, the shrimp were injected intramuscularly at the fifth abdominal segment with IMNV isolates (100 µL/shrimp) and observed for seven days post-infection (dpi). The parameters observed included hemolymph profile, survival rate, gross clinical signs, and water quality. IMNV infection was confirmed through both external clinical signs and PCR tests. The results showed that the addition of L. japonica water extract to the feed improved the survival rate and immune responses of whiteleg shrimp following the IMNV challenge test. In conclusion, dietary supplementation of L. japonica water extract at a concentration of 2% w/w in artificial feed could serve as a preventive agent against IMNV infection in whiteleg shrimp.
Co-Authors Ahmad Muzaki Bejo Slamet DARMAWAN SETIA BUDI Haryanti Haryanti Imlani, Ainulyakin Joko Sumarwan, Joko Loh, Jiun-Yan Mohammad Faizal Ulkhaq Muhammad Marzuqi Pardede, Maria Agustina Siti Subaidah Tatam Sutarmat