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Joko Sumarwan, Joko
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Dietary Honeysuckle (Lonicera japonica) Extract Enhances Resistance to Infectious Myonecrosis Virus in Whiteleg Shrimp (Litopenaeus vannamei) Subaidah, Siti; Sumarwan, Joko; Marzuqi, Muhammad; Tridjoko, Tridjoko; Slamet, Bejo; Budi, Darmawan Setia; Pardede, Maria Agustina; Ulkhaq, Mohammad Faizal; Imlani, Ainulyakin; Loh, Jiun-Yan
Jurnal Medik Veteriner Vol. 8 No. 2 (2025): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss2.2025.286-296

Abstract

This study aimed to investigate the immune response of whiteleg shrimp (Litopenaeus vannamei) to prevent infectious myonecrosis virus (IMNV) infection by incorporating Lonicera japonica water extract into artificial feed. A total of 180 whiteleg shrimp were used in four treatment groups (triplicate) receiving different doses of L. japonica extract in their feed, including treatment A (1% w/w), B (2% w/w), C (3% w/w), and D (without supplementation/control). The dietary treatments were applied twice daily for two weeks. On the 15th day, the shrimp were injected intramuscularly at the fifth abdominal segment with IMNV isolates (100 µL/shrimp) and observed for seven days post-infection (dpi). The parameters observed included hemolymph profile, survival rate, gross clinical signs, and water quality. IMNV infection was confirmed through both external clinical signs and PCR tests. The results showed that the addition of L. japonica water extract to the feed improved the survival rate and immune responses of whiteleg shrimp following the IMNV challenge test. In conclusion, dietary supplementation of L. japonica water extract at a concentration of 2% w/w in artificial feed could serve as a preventive agent against IMNV infection in whiteleg shrimp.
Perkawinan Resiprokal Udang Vaname (Litopenaeus vannamei) untuk Mendapatkan Generasi Terbaik pada Seleksi Famili Sumarwan, Joko; Anwar, Kasful; Fatimah, Fatia
Buletin Jalanidhitah Sarva Jivitam Vol 7, No 1 (2025): Maret 2025
Publisher : POLITEKNIK AHLI USAHA PERIKANAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/bjsj.v7i1.15561

Abstract

Penelitian ini bertujuan untuk mendapatkan 11 famili calon induk yang terbaik berdasar sintasan PL10. Tiga sumber Daya Genetik/SDG (Aa,Bb,Cc) yang masing-masing 120 pasang dikawinkan resiprokal. Sebagai kontrol dikawinkan SDG Kk yang terdiri 15 pasang. Perkawinan dilakukan di 8 bak @10 m2. Pemijahan dilakukan di 40 bak @220 liter. Pakan induk berupa cacing laut dan tiram. Pemeliharaan larva dilakukan pada 50 bak @600 liter dengan kepadatan 100 ekor/liter. Pakan larva berupa fitoplankton, pakan buatan dan nauplius artemia. Pemijahan selama 3 hari menghasilkan induk matang gonad 72 ekor (7,6%/hari), induk kawin 34 ekor (47,2%), induk mijah 32 ekor (94,1%), rerata fekunditas 157.063±36.565 butir/induk, produksi naupli 106.414±41.399 ekor/induk dengan daya tetas 69,4±21,7%. Dari 32 pemijahan yang mampu menetas 29 famili, nauplius yang layak ditebar terdiri 27 famili dan menghasilkan 22 famili PL10 dengan rerata sintasan 60,2±20,3%. Dari 22 famili diambil 11 famili keturunan induk hasil seleksi dengan sintasan terbaik dan 1 famili kontrol yaitu Ca1;Cc1;Ca3;Aa2;Bc2;Cb2;Bb3;Cc4;Ab2;Ab3;Cc2 dan Kk2 dengan sintasan masing-masing famili 89%;86%;79%;77%;76%;75%;71%;70%;70%;68% dan 63%.
Co-Authors Bejo Slamet DARMAWAN SETIA BUDI Fatia Fatimah Imlani, Ainulyakin Kasful Anwar Loh, Jiun-Yan Mohammad Faizal Ulkhaq Muhammad Marzuqi Pardede, Maria Agustina Siti Subaidah Tridjoko Tridjoko