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All Journal Jurnal Medik Veteriner Jurnal Mina Sains Jurnal Segara Journal of Aquaculture and Fish Health Abdimas: Jurnal Pengabdian Masyarakat Universitas Merdeka Malang Jurnal Pembelajaran Pemberdayaan Masyarakat (JP2M) Juvenil: Jurnal Ilmiah Kelautan dan Perikanan Jurnal Perikanan
Pardede, Maria Agustina
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Environmental Science Materials Science & Nanotechnology Social Sciences Veterinary

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Chloramphenicol Residues and Bacterial Contamination in Farmed African Catfish (Clarias gariepinus) from Banyuwangi Traditional Markets: A Risk Assessment Ulkhaq, Mohammad Faizal; Kenconojati, Hapsari; Budi, Darmawan Setia; Pardede, Maria Agustina; Loh, Jiun-Yan
Jurnal Medik Veteriner Vol. 7 No. 2 (2024): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol7.iss2.2024.244-254

Abstract

This study aimed to determine chloramphenicol residues and bacterial contamination (Aerobic Plate Count-APC, Escherichia coli count, Salmonella sp., and Vibrio cholerae) in farmed African catfish (Clarias gariepinus) that are marketed in Banyuwangi, Indonesia. A total of ninety samples of C. gariepinus were collected from several markets in Banyuwangi, namely, Kertosari, Blambangan, and Banyuwangi Kota. Using standard procedures, APC, E. coli count, Salmonella sp., V. cholerae, and chloramphenicol residues were determined. 36.37% of samples from Kertosari markets; 23.33% of samples from Banyuwangi Kota markets; and 16.67% of samples from Blambangan markets contained chloramphenicol residues, but less than 0.3 ppb. Only 40% of samples from Kertosari markets, 36.67% of samples from Banyuwangi Kota markets, and 26.67% of samples from Blambangan markets were contaminated by E. coli, but were less than 3 MPNg-1. The highest APC was from Banyuwangi Kota, followed by Kertosari and Blambangan. No samples were contaminated by Salmonella sp. and V. cholerae from any of the markets, and there was no significant difference between all markets in APC, E. coli count, or chloramphenicol residues. All farmed catfish marketed in Banyuwangi were safe to eat because no samples exceeded the maximum chloramphenicol residue and bacterial contaminant standards. Further studies are needed to detect other antibiotic residues used in aquaculture, including tetracycline, sulphonamide, enrofloxacin, and nitrofuran.
Economic improvement and fish farming based on Smart Aquaculture Automatized System in Segobang Village, Banyuwangi Fasya, Arif Habib; Kenconojati, Hapsari; Budi, Darmawan Setia; Suciyono, Suciyono; Pardede, Maria Agustina; Kumalaningrum, Dwi Retna; Prayogo, Prayogo; Saputra, Eka; Firdaus, Aji Akbar; Maulana, Muhammad Hilmy; Ambarwati, Dewi
Abdimas: Jurnal Pengabdian Masyarakat Universitas Merdeka Malang Vol. 10 No. 1 (2025): February 2025
Publisher : University of Merdeka Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26905/abdimas.v10i1.14811

Abstract

The fisheries and marine sector in East Java has abundant potential, one of which is Banyuwangi district. Banyuwangi Regency has potential in freshwater aquaculture because it has 324 rivers with an area of 735 km2 and swamps and reservoirs of 60 hectares. One of the villages in Banyuwangi Regency is Segobang Village, which has the potential to have abundant water sources and continuous flow. This potential can be utilized by training and assisting fish nurseries in Segobang Village by utilizing continuously flowing water and Smart Aquaculture Automatized System (SAAS) technology by utilizing biofilters and running water and aeration as a source of oxygen.  The stages carried out in this community service are as follows: initial assessment of the location, infrastructure and technology development, periodic training and mentoring, implementation and monitoring of the cultivation process, and continued marketing and sales. After the training, the Segobang Village community showed a significant increase in knowledge. The average pre-test scores ranged from 30 to 51, while the post-test scores increased to 79 to 87. This improvement shows that participants better understand the SAAS and its use in fish farming. Continued training and practice can help strengthen the adoption of this technology in the community.
CORAL REEF CULTIVATION AT PT. SRI KANDI AQUARIUM, BANYUWANGI: ANALYSIS OF STAGES AND RESULTS OF TRANSPLANTATION of Acropora formosa, Acropora granulosa, and Euphyllia glabrescens Bunayya, Faiq Hanif; Yuniardhi, Dimas Indra; Satria, Rizal Surya; Rahmatulloh, Alfariz Alif; Pardede, Maria Agustina
Jurnal Perikanan Unram Vol 15 No 2 (2025): JURNAL PERIKANAN
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jp.v15i2.1405

Abstract

Indonesia is a region known for its abundant natural resources, such as coral reefs. Coral reef ecosystems in Indonesia have begun to decrease, it was recorded that 35.15% of coral reefs in Indonesia entered into poor status or declared damaged. This is due to several factors such as damage caused by the illegal trade of coral reefs carried out by unscrupulous entrepreneurs because of the illegal trade of coral reefs, knowledge and skills are needed regarding transplantation methods in the cultivation of A. formosa, A. granulosa and E. glabrescens to find out the stages and results of transplantation methods in coral reef cultivation. This study was conducted at PT Sri Kandi Aquarium, Banyuwangi Regency, East Java on June 24, 2024 until August 24, 2024. The primary data collection methods used include observation and active participation, while for secondary data using literature studies. The transplantation process in coral reef cultivation consists of several activities, namely the preparation of transplantation tools and materials, making transplantation media, cutting parent fragments, transplantation, maintenance and harvesting. The results of transplantation are then classified based on sizes S, M, L and XL. Thus the transplantation method can be carried out in coral reef cultivation which consists of several stages including preparation of transplantation tools and materials, making transplantation media, cutting coral fragments, attaching the results of cutting fragments to the substrate, sowing corals on the transplantation table under the sea and periodic monitoring and maintenance of corals. The transplantation results obtained will be categorized based on size, namely S, M, L and XL.
Pathology of Silver Rasbora (Rasbora argyrotaenia) after Experi-mentally Infection with Edwardsiella tarda : Edwardsiellosis in Silver Rasbora Nindi, Inda Arsyi; Febrina, Melinda; Rahmawati, Anggi Rusita; Ulkhaq, Mohammad Faizal; Tjahjaningsih, Wahju; Kusdarwati, Rahayu; Pardede, Maria Agustina; Kumalasari, Ditta Putri; Loh, Jiun-Yan
Journal of Aquaculture and Fish Health Vol. 14 No. 2 (2025): JAFH Vol. 14 No. 2 June 2025
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v14i2.70433

Abstract

The high demand for silver rasbora (Rasbora argyrotaenia) from the wild had led to declining resources, making fish cultivation necessary. One of the challenges in fish farming is diseases caused by bacteria, such as Edwardsiella tarda, which can lead to Edwardsiellosis and result in mass mortality among fish. This study aimed to analyze the pathology of silver rasbora after experimental infection with E. tarda. Four hundred silver rasbora (5.4±7 cm and 0.47±2.63 g) were reared in twenty aquariums. The treatments consisted of four groups with five replicates, including immersion in E. tarda 1011 CFU/ml (P1), 1012 CFU/ml (P2), 1013 CFU/ml (P3), and a negative control (without E. tarda) (P0) for a 14-days rearing period. Parameters observed included survival rate, stress responses (plasma cortisol, blood glucose, and ventilation rate); hematology profile (total erythrocytes, total leukocytes, differential leukocytes, and hemoglobin levels), histopathological alterations in organs, survival rate, and clinical symptoms. The results showed that immersion in E. tarda did not affect fish mortality. However, other parameters, including hematological profile, stress responses, and histopathological alterations, showed increased values and several pathological changes, yet remained within normal limits.
Identification, Prevalence and Intensity of Ectoparasite Protozoa in Litopenaeus vannamei at Intensive and Traditional Shrimp Pond Ambarwati, Dewi; Pardede, Maria Agustina; Puspita Sari, Putri; Kurnia Agesti, Elmira; Aufa, In'amul; Abdullah, Taufiq; Kumalasari, Ditta Putri
JURNAL MINA SAINS Vol. 11 No. 1 (2025): Jurnal Mina Sains
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/jmss.v11i1.17413

Abstract

This study investigated the prevalence, diversity, and intensity of ectoparasitic protozoa infecting Litopenaeus vannamei in traditional and intensive shrimp ponds. A total of 25 shrimp from each pond type were examined for the presence of ectoparasitic protozoa. Four parasite species were identified in traditional ponds: Zoothamnium sp., Epistylis sp., Vorticella sp., and Trichodina sp. In contrast, three species (Zoothamnium sp., Epistylis sp., and Vorticella sp.) were found in intensive ponds, with Trichodina sp. absent in the latter. The prevalence of ectoparasitic protozoa was higher in intensive ponds, with 44% of shrimp infected, compared to 8% in traditional ponds. The intensity of infection was also higher in intensive ponds, with an average intensity of 10.27, categorized as medium, compared to an intensity of 5.2 in traditional ponds, categorized as low. These findings suggest that intensive farming conditions may contribute to increased susceptibility to ectoparasitic infections in shrimp. The study emphasizes the importance of monitoring and managing parasite infestations to reduce health risks in shrimp aquaculture systems.  
Peningkatan asupan protein hewani santri melalui edukasi gizi dan budidaya ikan di pondok pesantren Fasya, Arif Habib; Kenconojati, Hapsari; Budi, Darmawan Setia; Suciyono; Pardede, Maria Agustina; Kumalaningrum, Dwi Retna; Prayogo; Mauludia, Farida; Asyabil, Alin; Maulana, Muhammad Hilmy; Ambarwati, Dewi
Jurnal Pembelajaran Pemberdayaan Masyarakat (JP2M) Vol. 6 No. 3 (2025)
Publisher : Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/jp2m.v6i3.23872

Abstract

Pengabdian kepada masyratakat ini dilaksanakan di Pondok Pesantren Al Anwari, Banyuwangi sebagai upaya untuk memenuhi kebutuhan protein hewani bagi para santri melalui budidaya ikan lele. Tujuan dari kegiatan ini adalah untuk memenuhi asupan protein hewani pada pondok pesantren serta  meningkatkan pengetahuan dan keterampilan santri dalam aspek teknis budidaya meliputi manajemen pakan hingga pasca panen. Metode kegiatan meliputi lima tahap yaitu penjaringan aspirasi, pelatihan, pendampingan, monitoring dan evaluasi serta dilakukan pengukuran kualitas air serta pretest maupun posttest. Pelaksanaan kegiatan melibatkan 49 santri kelas IX bersama pengelola pondok pesantren. Hasil pengukuran kualitas air menunjukkan nilai berada dalam kisaran normal untuk budidaya ikan lele. Nilai pretest berkisar antara 86,64-94,74 meningkat menjadi 88,60-96,49 pada posttest. Hasil ini menunjukkan bahwa pendekatan berbasis praktik dan pendampingan efektif dalam meningkatkan pengetahuan peserta. Kegiatan ini berpotensi dilakukan di lembaga pendidikan berbasis pesantren lainnya untuk mendukung ketahanan pangan dan meningkatkan ketrampilan santri.
Dietary Honeysuckle (Lonicera japonica) Extract Enhances Resistance to Infectious Myonecrosis Virus in Whiteleg Shrimp (Litopenaeus vannamei) Subaidah, Siti; Sumarwan, Joko; Marzuqi, Muhammad; Tridjoko, Tridjoko; Slamet, Bejo; Budi, Darmawan Setia; Pardede, Maria Agustina; Ulkhaq, Mohammad Faizal; Imlani, Ainulyakin; Loh, Jiun-Yan
Jurnal Medik Veteriner Vol. 8 No. 2 (2025): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss2.2025.286-296

Abstract

This study aimed to investigate the immune response of whiteleg shrimp (Litopenaeus vannamei) to prevent infectious myonecrosis virus (IMNV) infection by incorporating Lonicera japonica water extract into artificial feed. A total of 180 whiteleg shrimp were used in four treatment groups (triplicate) receiving different doses of L. japonica extract in their feed, including treatment A (1% w/w), B (2% w/w), C (3% w/w), and D (without supplementation/control). The dietary treatments were applied twice daily for two weeks. On the 15th day, the shrimp were injected intramuscularly at the fifth abdominal segment with IMNV isolates (100 µL/shrimp) and observed for seven days post-infection (dpi). The parameters observed included hemolymph profile, survival rate, gross clinical signs, and water quality. IMNV infection was confirmed through both external clinical signs and PCR tests. The results showed that the addition of L. japonica water extract to the feed improved the survival rate and immune responses of whiteleg shrimp following the IMNV challenge test. In conclusion, dietary supplementation of L. japonica water extract at a concentration of 2% w/w in artificial feed could serve as a preventive agent against IMNV infection in whiteleg shrimp.
IDENTIFICATION, ISOLATION, PURIFICATION, GRAM STAINING, AND BIOCHEMICHAL TESTING PROCESSES OF AQUATIC BACTERIA FROM DIFERENT SOURCES Pardede, Maria Agustina; Fasya, Arif Habib; Kumalaningrum, Dwi Retna; Ulkhaq, Mohammad Faizal; Kenconojati, Hapsari; Ambarwati, Dewi; Maulana, Muhammad Hilmy
Jurnal Segara Vol 19, No 3 (2024): December
Publisher : Politeknik Kelautan dan Perikanan Dumai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/segara.v19i3.15409

Abstract

Abstract
Analysis of Hematological Profile of Catfish (Clarias sp.) as a Health Indicator Use of Hemolyzer for Aquatic Animals Pardede, Maria Agustina; Aziz, Muhammad Abdul; Apriliani, Dian Putri; Kumalaningrum, Dwi Retna; Ambarwati, Dewi; Maulidia, Farida; Asyabil, Alin; Maulana, Muhammad Hilmy
Juvenil Vol 6, No 4: November (2025)
Publisher : Department of Marine and Fisheries, Trunojoyo University of Madura, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/juvenil.v6i4.27494

Abstract

ABSTRACTThe hematological profile of catfish (Clarias sp.) is a critical indicator of health and well-being in aquaculture practices. This study aimed to analyze the hematological parameters of catfish under aquaculture conditions using two different hematology analyzers: Hemolyzer 3 Pro and Veterinary Hematology Analyzer Mindray Series BC2800Vet. Blood samples were collected from the caudal vein, anticoagulated with EDTA, and analyzed for red blood cell count (RBC), white blood cell count (WBC), hemoglobin (Hb), hematocrit (Hct), mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC), and platelet count (PLT). Results indicated significant variations between the analyzers. The Hemolyzer 3 Pro indicated elevated levels, with Hb ranging from 15.7 to 16.7 g/dl, RBC from 2.66 to 2.79 × 10^6/mm³, and WBC from 92,200 to 98,040 × 10^6/mm³, suggesting possible polycythemia and an active immune response. In contrast, the Mindray Analyzer reported values within the normal range, with Hb at 7.2 g/dl, RBC at 1.43 × 10^6/mm³, and WBC at 46,100 × 10^6/mm³, implying stable health conditions. The study also observed monocytosis, indicative of potential chronic inflammation or infection. These discrepancies highlight the importance of standardized methodologies in hematological assessments. Regular monitoring of hematological parameters is essential for early disease detection and effective management in aquaculture. Further research is recommended to explore the underlying causes of these variations and to optimize health management strategies for catfish in aquaculture systems.Keywords: Aquaculture, Catfish, Haemolyzer, Hematology
Co-Authors Ambarwati, Dewi Apriliani, Dian Putri Asyabil, Alin Aufa, In'amul Bejo Slamet Bunayya, Faiq Hanif DARMAWAN SETIA BUDI Dwi Retna Kumalaningrum Eka Saputra Fasya, Arif Habib Febrina, Melinda Firdaus, Aji Akbar Imlani, Ainulyakin Joko Sumarwan, Joko Kenconojati, Hapsari Kumalasari, Ditta Putri Kurnia Agesti, Elmira Loh, Jiun-Yan Maulana, Muhammad Hilmy Maulidia, Farida Mauludia, Farida Mohammad Faizal Ulkhaq Muhammad Abdul Aziz Muhammad Marzuqi Nindi, Inda Arsyi Prayogo Prayogo, Prayogo Puspita Sari, Putri Rahmatulloh, Alfariz Alif Rahmawati, Anggi Rusita Satria, Rizal Surya Siti Subaidah Suciyono Suciyono, Suciyono Taufiq Abdullah, Taufiq Tjahjaningsih, Wahju Tridjoko Tridjoko Yuniardhi, Dimas Indra