Claim Missing Document
Check
Articles

Found 14 Documents
Search

IDENTIFIKASI ISOLAT FUNGI ENDOFIT LBKURCC43 BERDASAR SEKUENS ITS rDNA DARI UMBI TANAMAN DAHLIA (DAHLIA VARIABILIS) Sefni Hendris; Titania T Nugroho; - Saryono
Sistem Informasi Vol 5 No 2 (2015): Jurnal Photon
Publisher : LPPM Universitas Muhammadiyah Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37859/jp.v5i2.578

Abstract

Fungi LBKURCC43 merupakan fungi endofit yang diisolasi dari umbi tanaman dahlia berbunga ungu (Dahlia variabilis) di Padang Panjang, Sumatera Barat. Identifikasi secara morfologi isolat tersebut telah dilakukan dan hanya mengidentifikasi pada tingkat genus. Identifikasi secara molekuler dengan menggunakan DNA adalah identifikasi spesies yang lebih tepat digunakan. Sebelum dilakukan analisis filogenetik secara molekuler berdasarkan sekuens DNA ribosomal pada daerah ITS-1 dan ITS-2, dilakukan ekstraksi DNA dan amplikasi PCR ITS rDNA yang baik untuk sekuensing. DNA kromosomal diisolasi menggunakan kit Wizard Genomic Purification ex Promega Co (Madison, USA) dari sel miselia berumur tiga hari. Hasil penelitian menunjukkan bahwa DNA berhasil diisolasi dari miselia yang berumur tiga hari sebelum terbentuk spora dan jumlah yang cukup tinggi untuk PCR. DNA kromosomal fungi LBKURCC43 memiliki BM (berat molekul) 10.294 pb. ITS rDNA berhasil diamplifikasi dengan PCR menggunakan pasangan primer ITS5 dan ITS4, suhu annealing untuk 440C dan menghasilkan produk PCR dengan berat molekul 455 pb. Hasil analisis filogenetik daerah ITS-1, ITS-2 dan 5,8S rDNA dari genom fungi LBKURCC43 menunjukkan bahwa spesies dari fungi LBKURCC43 adalah Hanseniaspora uvarum dengan kemiripan identitas mencapai 97%.
Pelatihan dan Pemberdayaan Kader Posyandu dalam Pemanfaatan Makanan Fermentasi Tradisional Kuansing untuk Peningkatan Gizi Ibu Hamil dan Balita Ismawati, Ismawati; saryono, saryono; mukhyarjon, mukhyarjon; enikarmila Asni; Devi, Silvera; Anggraini, Suci; Suraya, Nabella; Kesya, Lunari; Salsabila, Dinda; Deanra, Cantika Natswa
Jurnal Pengabdian UntukMu NegeRI Vol. 9 No. 3 (2025): Pengabdian Untuk Mu negeRI
Publisher : LPPM UMRI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37859/jpumri.v9i3.10439

Abstract

Stunting in children remains a health problem in Indonesia. One way to improve the nutritional status of the community to prevent stunting is by utilizing nutrient-rich traditional fermented foods. This community service activity aimed to enhance the knowledge and skills of Posyandu cadres in utilizing conventional fermented foods from Kuansing as a nutritional source for pregnant women and toddlers. The training was conducted on August 30, 2025, at the Gunung Toar Sub-district Multipurpose Hall, Kuantan Singingi Regency, attended by 26 Posyandu cadres. The methods included educational video screening, booklet distribution, interactive discussions, and demonstrations of traditional fermented products such as pekasam, sarobuong, and puduong. Evaluation results showed an increase in participants' knowledge scores from an average of 50 to 70 after the training. This activity demonstrates the effectiveness of science-based education in improving community nutrition awareness and preserving local fermented foods.
OPTIMASI PERTUMBUHAN ISOLAT FUNGI INDIGEN Penicillium sp. LBKURCC153 PENDEGRADASI NAFTALENA MENGGUNAKAN GLUKOSA SEBAGAI KOSUBSTRAT PADA MINIMAL MEDIUM Aisyah Fitrida; Riryn Novianty; Saryono Saryono; Amir Awaluddin; Nova Wahyu Pratiwi
Jurnal Inovasi Pendidikan dan Sains Vol 1 No 1 (2020): April
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Nahdlatul Wathan Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Naphthalene is one of the pollutants that is difficult to degrade because it belongs to the PAH group and it was carcinogenic. The purpose of this study was to test the effectiveness of an indigenous fungi isolate from Riau Province to degrade naphthalene toxic compounds with glucose as growth cosubstrate. Naphthalene degradation by fungi isolates was influenced by several parameters, including biomass, pH and percentage degradation measured at 0, 4, 8, 12 and 16 days of incubation. Based on biomass parameters, the isolate of Penicillium sp. LBKURCC153 experienced an exponential phase until the 16th day. In the exponential phase, the maximum number of cells increases which shows a positive response to the use of glucose and naphthalene as the carbon and energy source. Isolate decreased their pH from 7 to 3,8, that mean proves the growth activity produce organic acid metabolites of degrade naphthalene. Isolate were also able to degrade naphthalene respectively 27,5% by Penicillium sp. LBKURCC153 in medium with glucose at optimation time until 4 th day incubation. In conclusion, glucose cosubstrate can increase naphthalene degradation percentage by Penicillium sp. LBKURCC153.
The Potential Of Fungi And Bacteria As α-Glucosidase Inhibitors For The Future Treatment Of Type 2 Diabetes Edy Fachrial; Ismawati; Afif P Jati; Titania T Nugroho; Saryono
Jurnal Penelitian Pendidikan IPA Vol 11 No 1 (2025): January
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i1.9457

Abstract

Diabetes, a disorder of hemostasis of carbohydrate and lipid metabolism, is one of today's leading killers. The most prevalent form of diabetes is type 2 diabetes mellitus (T2DM). Rapid hydrolysis of starch by pancreatic α-amylase and α-glucosidase, followed by intestinal absorption of glucose, causes a sudden increase in blood glucose. Available therapies for T2DM are oral insulin secretagogues, sulfonylureas, repaglinide, nateglinide, biguanides, thiazolidinediones, α-glucosidase, inhibitors and insulin. However, several hypoglycemic agents have limitations, such as side effects and increased diabetes complications. α-glucosidase inhibitors are structurally similar to natural oligosaccharides with a higher affinity for α-glucosidases, and they produce a reversible inhibition of membrane-bound intestinal α-glucoside hydrolase enzymes. This causes delayed carbohydrate absorption and digestion and results in a reduction in postprandial hyperglycemia. Natural α-glucosidase inhibitor drugs from natural sources can be used as a therapeutic approach to treat postprandial hyperglycemia for their assumed lower side effect and more affordable price than synthetic drugs. In this article, the author summarizes the potential of α-glucosidase inhibitors from microorganisms, namely fungi and bacteria, along with several active compounds with better activity than commercial α-glucosidase inhibitors.