Article Per Year (5 Year)
p-Index From 2020 - 2025
0.817
P-Index
This Author published in this journals
All Journal HAYATI Journal of Biosciences Jurnal Kimia (Journal of Chemistry) Jurnal Promosi Pendidikan Ekonomi Indonesian Journal Of Environmental Science and Technology Jurnal Penelitian Pendidikan IPA (JPPIPA) Chimica et Natura Acta JURNAL PHOTON Jurnal Inovasi Pendidikan dan Sains
Saryono
Universitas Riau
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Materials Science & Nanotechnology Nursing Physics

Published : 12 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 12 Documents
Search

 1   2 
IDENTIFIKASI ISOLAT FUNGI ENDOFIT LBKURCC43 BERDASAR SEKUENS ITS rDNA DARI UMBI TANAMAN DAHLIA (DAHLIA VARIABILIS) Sefni Hendris; Titania T Nugroho; - Saryono
Sistem Informasi Vol 5 No 2 (2015): Jurnal Photon
Publisher : LPPM Universitas Muhammadiyah Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37859/jp.v5i2.578

Abstract

Fungi LBKURCC43 merupakan fungi endofit yang diisolasi dari umbi tanaman dahlia berbunga ungu (Dahlia variabilis) di Padang Panjang, Sumatera Barat. Identifikasi secara morfologi isolat tersebut telah dilakukan dan hanya mengidentifikasi pada tingkat genus. Identifikasi secara molekuler dengan menggunakan DNA adalah identifikasi spesies yang lebih tepat digunakan. Sebelum dilakukan analisis filogenetik secara molekuler berdasarkan sekuens DNA ribosomal pada daerah ITS-1 dan ITS-2, dilakukan ekstraksi DNA dan amplikasi PCR ITS rDNA yang baik untuk sekuensing. DNA kromosomal diisolasi menggunakan kit Wizard Genomic Purification ex Promega Co (Madison, USA) dari sel miselia berumur tiga hari. Hasil penelitian menunjukkan bahwa DNA berhasil diisolasi dari miselia yang berumur tiga hari sebelum terbentuk spora dan jumlah yang cukup tinggi untuk PCR. DNA kromosomal fungi LBKURCC43 memiliki BM (berat molekul) 10.294 pb. ITS rDNA berhasil diamplifikasi dengan PCR menggunakan pasangan primer ITS5 dan ITS4, suhu annealing untuk 440C dan menghasilkan produk PCR dengan berat molekul 455 pb. Hasil analisis filogenetik daerah ITS-1, ITS-2 dan 5,8S rDNA dari genom fungi LBKURCC43 menunjukkan bahwa spesies dari fungi LBKURCC43 adalah Hanseniaspora uvarum dengan kemiripan identitas mencapai 97%.
The Potential Of Fungi And Bacteria As α-Glucosidase Inhibitors For The Future Treatment Of Type 2 Diabetes Edy Fachrial; Ismawati; Afif P Jati; Titania T Nugroho; Saryono
Jurnal Penelitian Pendidikan IPA Vol 11 No 1 (2025): January
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i1.9457

Abstract

Diabetes, a disorder of hemostasis of carbohydrate and lipid metabolism, is one of today's leading killers. The most prevalent form of diabetes is type 2 diabetes mellitus (T2DM). Rapid hydrolysis of starch by pancreatic α-amylase and α-glucosidase, followed by intestinal absorption of glucose, causes a sudden increase in blood glucose. Available therapies for T2DM are oral insulin secretagogues, sulfonylureas, repaglinide, nateglinide, biguanides, thiazolidinediones, α-glucosidase, inhibitors and insulin. However, several hypoglycemic agents have limitations, such as side effects and increased diabetes complications. α-glucosidase inhibitors are structurally similar to natural oligosaccharides with a higher affinity for α-glucosidases, and they produce a reversible inhibition of membrane-bound intestinal α-glucoside hydrolase enzymes. This causes delayed carbohydrate absorption and digestion and results in a reduction in postprandial hyperglycemia. Natural α-glucosidase inhibitor drugs from natural sources can be used as a therapeutic approach to treat postprandial hyperglycemia for their assumed lower side effect and more affordable price than synthetic drugs. In this article, the author summarizes the potential of α-glucosidase inhibitors from microorganisms, namely fungi and bacteria, along with several active compounds with better activity than commercial α-glucosidase inhibitors.
Co-Authors - Paslun A. Awaluddin Afif P Jati Aisyah Fitrida Amilia Linggawati Amir Awaliddin Amir Awaluddin B. Antika Chiristine Jose Edy Fachrial Fitriani Fitriani Gimin Gimin Ismawati N. W. Pratiwi Nadya Hermantika x Hermantika Sari, Nadya Hermantika x Nelvia Nelvia Nova Wahyu Pratiwi Novianty, Riryn R. Novianty R. Ukun M.S. Soedjanaatmadja Rosa Murwindra, Rosa Suardi Tarumun Suci Apriani Sumarno Sumarno Titania T Nugroho Titania Tjandrawati Nugroho Yayuk Sri Rejeki, Yayuk Sri Yuana Nurulita Zona Octarya