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All Journal Jurnal Ilmu Pertanian JOURNAL OF COASTAL DEVELOPMENT ILMU KELAUTAN: Indonesian Journal of Marine Sciences Ganendra: Majalah IPTEK Nuklir Microbiology Indonesia Journal of Tropical Soils Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Biotropika Indonesian Journal of Biotechnology Jurnal Perlindungan Tanaman Indonesia Journal of Tropical Biodiversity and Biotechnology
Triwibowo Yuwono
Laboratory Of Microbiology, Department Of Agricultural Microbiology, Faculty Of Agriculture, Universitas Gadjah Mada, Yogyakarta, Indonesia, 55281
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Other

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Profil Protein Klebsiella sp. dalam Kondisi Cekaman Osmotik dan Keasaman Ali Ikhwan; Triwibowo Yuwono; Jaka Widada
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 16, No 2 (2011): June 2011
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v16i2.94

Abstract

Penelitian dilakukan untuk mengetahui profil protein yang dibuat oleh Klebsiella sp. yang tumbuh dalam kondisi cekaman osmotik dan keasaman. Cekaman osmotik dilakukan menggunakan NaCl, sedangkan cekaman keasaman menggunakan aluminium sulfat. Klebsiella sp. ditumbuhkan dalam medium minimal yang ditambah dengan NaCl, atau aluminium sulfat, untuk menimbulkan efek cekaman tunggal, atau menggunakan kedua senyawa tersebut untuk menghasilkan efek cekaman ganda. Protein total yang diekstrak dari sel kemudian dielektroforesis pada SDS-PAGE 12%. Hasil analisis menunjukkan beberapa protein intraselular, protein membran, atau protein ekstraselular yang dibuat dalam kondisi cekaman spesifik. Dalam kondisi cekaman osmotik, dibuat protein intraselular berukuran 42,7 kDa, dan protein membran berukuran 53,3 kDa. Pada cekaman asam dihasilkan protein intraselular berukuran 54,7 kDa, 25,3 kDa, 14,2 kDa, dan satu protein membran berukuran 43,9 kDa, serta protein ekstraselular berukuran 17–29 kDa. Dalam kondisi cekaman ganda, terdeteksi satu protein intraselular spesifik berukuran 26,7 kDa dan satu protein membran berukuran 61,1 kDa. Dalam cekaman osmotik, diketahui terdapat korelasi positif, sedangkan dalam cekaman ganda terdapat korelasi negatif terhadap macam protein. Dalam cekaman keasaman, tidak diperoleh pola korelasi yang spesifik.
Karakter Populasi Wereng Hijau, Nephotettix virescens (Hemiptera: Cicadellidae) di Wilayah Endemi dan Non Endemi Penyakit Tungro Padi Supriyadi Supriyadi; Kasumbogo Untung; Andi Trisyono; Triwibowo Yuwono
Jurnal Perlindungan Tanaman Indonesia Vol 10, No 2 (2004)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12203

Abstract

The objectives of this research were to determine the species composition, distribution pattern and virus transmission properties of N. virescens. Sweep net sampling of the leafhopper and others tungro vectors population were conducted from endemic areas in Central Java, East Java and Bali and also non endemic areas from Central Java and East Java. The species composition and distribution pattern of the leafhopper were studied in seedbeds and early stage of rice crop (30 days after transplanting). Inoculation test using Cisadane seedlings was conducted to determine the efficiency of virus transmission by N. virescens colonies. Four tungro virus vectors were found in all areas, namely N. virescens, N. nigropictus, N. malayanus, and Recilia sp. The domination of N. virescens occurred either in the endemic area or the non endemic areas. However, the domination of N. virescens did not correlate with the occurrence of the disease in the endemic area. The distribution pattern of N. virescens was regular. The number of N. virescens active transmitter in the endemic area was higher than that in the non-endemic areas. In addition, the female of N. virescens was more efficient in transmitting the tungro virus than that of the male.
Pengaruh Minyak Atsiri Asal Kulit Jeruk Manis dan Besar terhadap Perkembangan Tungau Panonychus citri (Acarina: Tetranychidae) Mizu lstianto; Kasumbogo Untung; Y. Andi Trisyono; Triwibowo Yuwono
Jurnal Perlindungan Tanaman Indonesia Vol 9, No 2 (2003)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12244

Abstract

P. citri (Acarina: Tetranychidae) is one of the economically important citrus pests in Indonesia. However, the association of this pest with its host is not well understood. The objective of this research was to evaluate the effects of essential oil extracted from sweet orange and pumello fruit peels on the development of P. citri. The research was conducted in the laboratory of IP2TP Tlekung Malang, using a complete random design consisted of six treatments. Fifteen mites were used for each treatment. The treatments were concentrations 10, 20, 40, and 80 ppm of essential oil. Parafin was used as a positive control because it was a diluting solution for the essential oil. The control received no application. The result showed that the main content of essential oil extracted from sweet orange and pumello was limonene. The essential oils prolonged the life cycle and reduced the fecundity of P. citri. The effects of essential oil extracted from pumello were found to be more pronounced than were from sweet orange. These differences might be due to the differences in the composition of the volatile compounds other than limonene.
Molecular and Physiological Characterization of Copper-Resistant Bacteria Isolated from Activated Sludge in an Industrial Wastewater Treatment Plant in Rungkut-Surabaya, Indonesia WAHYU IRAWATI; TRIWIBOWO YUWONO; JOEDORO SOEDARSONO; HARI HARTIKO
Microbiology Indonesia Vol. 6 No. 3 (2012): September 2012
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (850.788 KB) | DOI: 10.5454/mi.6.3.3

Abstract

Copper resistant bacteria can be isolated from environments where copper levels are abundant from mining, industrial, or agricultural activities. The aim of this work was to study the molecular and physiologicalcharacteristics of indigenous copper resistant bacteria isolated from activated sludge in an industrial wastewatertreatment plant in Surabaya, Indonesia. The bacterial isolates were designated as strains IrC1, IrC2, and IrC4. Phylogenetic analysis based on 16S rDNA sequence analysis identified isolates IrC1, IrC2, and IrC4 as Acinetobacter oleivorans (98.41% similarity), Acinetobacter pitii (97.22% similarity), and Cupriavidus pauculus (96.99 similarity), respectively. The addition of 5 mM of CuSO4 in the medium affected morphological 4 appearance of all isolates to green and undulate margin might be due to the survival mechanism of bacteria by absorbing the copper. This studies indicated that copper resistance mechanism of all isolates was facilitated through the bioaccumulation of copper inside the cell, especially on the membrane fraction and inside the cytoplasm, albeit at a limited amount. It was observed that isolates IrC1, IrC2, and IrC4 were capable of accumulating 137.23 , 364.66 , and 272.07 mg L-1 of copper, respectively from the medium containing 8 mM CuSO4. The capability of isolates IrC1, IrC2, and IrC4 to accumulate copper can be exploited in bioremediation 4 process for removing copper from industrial sewage.
Growth and Physiological Attributes of Rice by the Inoculation of Osmotolerant Rhizobacteria (Enterobacter flavescens) under Drought Condition Hasna Dyah Kusumardani; Triwibowo Yuwono; Diah Rachmawati
Journal of Tropical Biodiversity and Biotechnology Vol 7, No 2 (2022): August
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.67359

Abstract

Rice (Oryza sativa L.) has mechanism for morphological, physiological, and biochemical self-defense in response to drought conditions. The ability of osmotolerant rhizobacteria to develop association with plants suggests that it could be used as an inoculum to support plant growth under drought stress. The purpose of this study is to determine the response of ‘IR64’ and ‘Situ Bagendit’ to the inoculation with osmotolerant rhizobacteria under drought conditions. The experiment had 3 treatment factors: 2 rice cultivars ('IR64' and 'Situ Bagendit'), 3 drought treatments (25%, 50% and 100% field capacity), and 2 types of rhizobacteria treatments (without inoculation and with inoculation using osmotolerant rhizobacteria (Enterobacter flavescens). Plant growth was measured in terms of plant height, number of leaves, number of tillers and panicles, and percentage of filled grain. Physiological and biochemical parameters, namely chlorophyll, carotenoids, proline, superoxide dismutase (SOD) peroxidase (POX) and ascorbate peroxidase (APX) were measured. The inoculation of osmotolerant rhizobacteria enhanced ‘IR64’ and ‘Situ Bagendit’ growth (plant height, number of leaves, tillers and panicles) and increased the percentage of grains in ‘IR64’ cultivar. Proline content, SOD, and APX activities were all increased by osmotolerant rhizobacteria inoculation, however, carotenoid content was decreased. Plant growth, physiological and biochemical responses of both cultivar to drought were enhanced by inoculation with osmotolerant rhizobacteria. 
Effect of Coated Urea with Humic-Calcium on Transformation of Nitrogen in Coastal Sandy Soil: A Soil Column Method Sulakhudin, Sulakhudin; Syukur, Abdul; Shiddieq, Dja'far; Yuwono, Triwibowo
JOURNAL OF TROPICAL SOILS Vol. 15 No. 1: January 2010
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2010.v15i1.11-18

Abstract

Effect of Coated Urea with Humic-Calcium on Transformation of Nitrogen in Coastal Sandy Soil: A Soil Column Method (Sulakhudin, A  Syukur, D  Shiddieq and T  Yuwono):  In coastal sandy soil, mainly nitrogen losses due to leaching resulted to low fertilizer efficiency. Slow-release N fertilizers are proposed to minimize these losses, and humic-calcium coated urea has been examined. A soil column method was used to compare the effects of coated urea with humic-calcium on transformation and leaching loss of N in coastal sandy soil. The experiment aid to compare two kind source humic substances (cow manure and peat) which mixed with calcium as coated urea on transformation, vertical distribution and leaching N in coastal sandy soil. The concentration of humic-calcium coated urea i.e.1%, 5% and 10% based on their weight.  The results showed that urea coated with humic-calcium from cow manure (UCHM) and humic-calcium from peat (UCHP) increased the N total and available N in the soil and decreased leaching loss of N from the soil column. Compare to UCHP, UCHM in all concentration showed N-nitrate higher than N-ammonium on incubation length 2, 4 and 6 weeks. The N leached from a costal sandy soil with application coated urea with UCHM ranged from 21.18% to 23.72% of the total N added as fertilizer, for coated urea with UCHP they ranged between 21.44% and 23.25%, whereas for urea (control) reach 29.48%. Leaching losses of mineral N were lower when urea coated with UCHM compared to urea coated with UCHP or urea fertilizer.  The study concluded that the UCHM is better than UCHP in decreasing N leached from coastal sandy soil.
Co-Authors Abdul Syukur Agus Taftazani Agus Taftazani Ali Ikhwan Arofatullah, Nur Akbar Bernadetta Octavia Diah Rachmawati Donny Widianto Endang Kusdiyantini Endang Kusdiyantini Endang Pamulatsih Erni Martani HARI HARTIKO Hasna Dyah Kusumardani Hermin Pancasakti Kusumaningrum Hermin Pancasakti Kusumaningrum Ikhwan , Ali Irfan Dwidya Prijambada JAKA WIDADA JOEDORO SOEDARSONO JOEDORO SOEDARSONO Joedoro Sudarsono Kasumbogo Untung M. Saifur Rohman Mizu lstianto Shiddieq, Dja'far Siti Subandiyah Sulakhudin Sulakhudin Supriyadi Supriyadi WAHYU IRAWATI Wayan T. Artama Widada , Jaka Y. Andi Trisyono Yudi Kusnadi