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H.I. Wahyuni
Faculty of Animal Agriculture Diponegoro University Tembalang Campus, Semarang 50275
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

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The potential of Bacillus strains isolated from the rumen content of dairy cows as natural antibacterial and antioxidant agents for broilers Sugiharto, S.; Yudiarti, T.; Isroli, I.; Widiastuti, E.; Wahyuni, H.I.; Suprijatna, E.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 2 (2018): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.2.115-123

Abstract

The study was conducted to investigate the in vitro antibacterial activity and antioxidant-enhancing effect of Bacillus strains isolated from the rumen content of dairy cows. The antibacterial activity of Bacillus strains was assessed against Escherichia colibased on the agar-well diffusion method. To assess the antioxidant-enhancing effect, the Bacillusstrains was mixed (prepared as multi-strains) and used to ferment herbal medicine waste (HMW). The effect was compared with that of commercial probiotic Bacillus subtilis and non fermented HWM. Results showed that each Bacillus strain was able to inhibit the growth of E. coli. Fermentation with mixed Bacillus strains tended (P=0.07) to enhance the 2,2-diphenylpicrylhydrazyl (DPPH) radicals scavenging activity and total phenolics content of HMW. In contrast, fermentation with commercial Bacillus subtilis tended (P=0.07) to decrease DPPH radical scavenging activity and total phenol of HMW. Fermentation with both Bacillus preparations increased (P<0.05) the contents of crude protein and ash in HMW, while crude fat increased  (P<0.05) in HMW fermented with Bacillus subtilis. Fermentation either with Bacillus subtilis or mixed Bacillus strains increased (P<0.05) the fiber content of HMW. In conclusion, Bacillus strains isolated from the rumen content of dairy cows showed antibacterial activity and antioxidant-enhancing effect, therefore, they are potential as feed additive to substitute synthetic antibiotics and antioxidants for broilers raised under tropical conditions.
EGG QUALITY AND HATCHABILITY OF In situ - REARED KEDU AND CEMANI HENS FED DIET OF FARMER FORMULATION SUPPLEMENTED WITH VITAMIN E Wahyuni, H.I.; Suthama, N.; Mangisah, I.; Sarjana, T.A.
Journal of the Indonesian Tropical Animal Agriculture Vol 36, No 1 (2011): (March)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.36.1.61-68

Abstract

The aim of study was to evaluate the effect of dietary vitamin E supplementation in Kedu andCemani hens reraed in situ and given farmer-formulated diet on egg performances. A total of 120 femaleand 24 male birds were equally divided into two groups of Kedu and Cemani, with average body weightof 1890+216.79 and 1830+396.23 g, respectively. Basal ration was based on the diet formulated by thefarmer (R1) consisting of corn (30%), rice bran (50%), protein concentrate (15%), and premix (5%).Dietary treatments evaluated were R1 (without additional vitamin E), and the other three were theinclusion of DL α-tocopheryl acetate into the R1 diet up to 2 (R2), 4 (R3) and 6 IU (R4). The experimentwas arranged in a Randomized Block Design with 4 treatments, and 2 different groups of hen (Kedu andCemani) were assigned as block. Parameters observed were feed consumption, hen day production, feedconversion ratio, egg quality, fertility, and hatchability. The result showed that vitamin Esupplementation did not affect whatever parameters, except egg fertility. Egg hatchability between Keduand Cemani hens was significantly different (p<0.05). Trend comparison test indicated significantlylinear (p<0.05) for fertility and hatchability. In conclusion, vitamin E supplementation up to 6 IUincreased linearly egg fertility and hatchability of about 5-8%, and egg hatchability of Kedu hens washigher (11%) than that of Cemani.
Egg's vitamin E deposition of Kedu breeder chicken fed improved diets Wahyuni, H.I.; Suthama, N.; Mangisah, I.; Krismiyanto, L.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 4 (2018): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.4.421-428

Abstract

The research aimed to evaluate dietary vitamin E deposited into the egg of Kedu breeder hen reared in situ and fed an improved diet supplemented with vitamin E. A total of 75 breeder hens and 15 cockerel of Kedu chicken aged ± 12 months, with average body weight of 1667 ± 237 and 2295 ± 250 g/bird, respectively, were distributed into 15 units of semi intensive housing system. A completely randomized designed (CRD) was arranged in the present research with 3 dietary treatments, namely farmer formulated ration (T0), improved ration (T1), and T1 + 6.28 mg/kg vitamin E (T2). Parameters observed were feed consumption, consumption and retention of vitamin E, egg’s vitamin E, egg’s vitamin E deposition efficiency, hen day production (HDP) and egg weight. All data gathered were subjected to analysis of variance and Duncan’s multiple range test to differentiate between treatments. Variables, except feed consumption, HDP and egg weight, were significantly (P<0.05) influenced by dietary treatment. Vitamin E consumption and retention, and deposition increased due to feeding effect of improved diet and supplementation of vitamin E. Dietary vitamin E of farmer’s ration deposited into the egg indicated the highest value (229.23%) followed by improved ration (51.85%), and improved ration + vitamin E was the lowest (5.38%). In conclusion, Kedu hen is likely have a limitation on vitamin E deposited into the egg when the improved ration supplemented with vitamin E is fed, and vitamin E supplementation should be implemented together with the increase of fat content in the ration.
Co-Authors E. Suprijatna, E. E. Widiastuti I. Isroli I. Mangisah Lilik Krismiyanto N. Suthama S. Sugiharto T.A. Sarjana Turrini Yudiarti