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All Journal Nexus Biomedika Media Dermato-Venereologica Indonesiana
Irene Ardiani Pramudya Wardhani
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Biochemistry, Genetics & Molecular Biology Health Professions

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Efek Antifungi Seduhan Teh Hijau (Camellia sinensis L.) terhadap Pertumbuhan Candida albicans In Vitro Wardhani, Irene Ardiani Pramudya; Haryati, Sri; Setyawan, Novan Adi
Nexus Biomedika Vol 1, No 2 (2012): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.858 KB)

Abstract

Background : Brewed green tea contains antifungal substance such as polyphenol which is considered can inhibit fungal growth.  The aim of this research is to determine antifungal effect of brewed green tea (Camellia sinensis L.) against Candida albicans growth In Vitro. Methods : The research was performed as experimental laboratory. The subject of this research were clinical sample of Candida albicans and taken randomly. This research used 7 treatment groups, they were sterilized aquadest as negative control, brewed green tea with concentration of 60%, 70%, 80%, 90% and 100%, also fluconazole 25 µg as positive control. This research was repeated four times. The plate was incubated at the temperature of 37ºC for 48 hours then inhibition diameter zone formed was measured. The data was analyzed by Linier Regression test then continued by Correlation test on IBM Statistics for Windows version 20. Results : The diameter of inhibition zone increased along with increasing concentrations, because higher concentration of brewed green tea contained more substances, and the diameter of concentration of 100% group is almost the same with diameter of positive control group. The results of statistical analysis using Linier Regression test showed that there are significant differences on seven of treatment groups (p < 0,05)  and the Correlation test showed that there are close relation between concentration of brewed green tea and the diameter of inhibition zone. Conclusion : Brewed green tea (Camellia sinensis L.) has antifungal effect against Candida albicans growth In Vitro. Start from concentration of 60% up to 100%, there were antifungal effect, and the antifungal effect of concentration of 100% is close to  fluconazole  25 µg/ml. Keywords : Antifungal Effect, Brewed Green Tea, Candida albicans 
Efek Antifungi Seduhan Teh Hijau (Camellia sinensis L.) terhadap Pertumbuhan Candida albicans In Vitro Irene Ardiani Pramudya Wardhani; Sri Haryati; Novan Adi Setyawan
Nexus Biomedika Vol 1, No 2 (2012): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.858 KB)

Abstract

Background : Brewed green tea contains antifungal substance such as polyphenol which is considered can inhibit fungal growth. The aim of this research is to determine antifungal effect of brewed green tea (Camellia sinensis L.) against Candida albicans growth In Vitro. Methods : The research was performed as experimental laboratory. The subject of this research were clinical sample of Candida albicans and taken randomly. This research used 7 treatment groups, they were sterilized aquadest as negative control, brewed green tea with concentration of 60%, 70%, 80%, 90% and 100%, also fluconazole 25 g as positive control. This research was repeated four times. The plate was incubated at the temperature of 37C for 48 hours then inhibition diameter zone formed was measured. The data was analyzed by Linier Regression test then continued by Correlation test on IBM Statistics for Windows version 20. Results : The diameter of inhibition zone increased along with increasing concentrations, because higher concentration of brewed green tea contained more substances, and the diameter of concentration of 100% group is almost the same with diameter of positive control group. The results of statistical analysis using Linier Regression test showed that there are significant differences on seven of treatment groups (p < 0,05) and the Correlation test showed that there are close relation between concentration of brewed green tea and the diameter of inhibition zone. Conclusion : Brewed green tea (Camellia sinensis L.) has antifungal effect against Candida albicans growth In Vitro. Start from concentration of 60% up to 100%, there were antifungal effect, and the antifungal effect of concentration of 100% is close to fluconazole 25 g/ml. Keywords : Antifungal Effect, Brewed Green Tea, Candida albicans
HUBUNGAN TINGKAT DEPRESI DENGAN DISFUNGSI EREKSI PADA KOMUNITAS LELAKI SEKS LELAKI (LSL) DI SURAKARTA Ervina Rosmarwati; Winda Wijayanti; Irene Ardiani Pramudya Wardhani; Ammarilis Murastami
Media Dermato-Venereologica Indonesiana Vol 50 No 1 (2023): Media Dermato-Venereologica Indonesiana
Publisher : Perhimpunan Dokter Spesialis Kulit dan Kelamin Indonesia (PERDOSKI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33820/mdvi.v50i1.407

Abstract

Pendahuluan: Disfungsi ereksi menjadi perhatian di golongan homoseksual dan sering dikaitkan dengan depresi sebagai salah satu pemicunya. Tujuan: Mengetahui hubungan antara tingkat depresi dengan kejadian disfungsi ereksi di komunitas Lelaki Seks Lelaki (LSL) di Surakarta. Metode: Penelitian analitik observasional dilakukan di Surakarta periode Juni-Juli 2021. Subjek adalah 41 laki-laki berusia 20-50 tahun, orientasi seksual dengan sesama jenis dan menyetujui untuk mengikuti penelitian. Penilaian tingkat depresi menggunakan kuisioner Patient Health Questionnaire-9 (PHQ-9). Penilaian disfungsi ereksi menggunakan kuisioner International Index of Erectile Function (IIEF) dalam domain fungsi ereksi. Analisis data menggunakan uji korelasi spearman rank SPSS versi 21 dengan nilai signifikasi p<0,05. Hasil: Tingkat depresi dan faktor usia tidak memiliki hubungan yang signifikan dengan disfungsi ereksi (r=0,158 dan p=0,323; r=0,020 dan p=0,901). Variabel yang berhubungan signifikan dengan kejadian disfungsi ereksi adalah frekuensi hubungan seksual (r= -0,389 dan p=0,012) dimana semakin jarang subjek penelitian melakukan hubungan seksual maka semakin tinggi kemungkinan terjadinya disfungsi ereksi. Tingkat koefisien korelasi antara frekuensi hubungan seksual terhadap kejadian disfungsi ereksi adalah kategori lemah (r=0,200-0,399). Kesimpulan: Tingkat depresi tidak berkorelasi terhadap kejadian disfungsi ereksi, namun kejadian disfungsi ereksi berkorelasi negatif dengan frekuensi hubungan seksual pada komunitas LSL di Surakarta.
Co-Authors Ammarilis Murastami Ervina Rosmarwati Novan Adi Setyawan Novan Adi Setyawan Sri Haryati Sri Haryati Winda Wijayanti