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A Ekstrak Etanolik Daun Kelor (Moringa oleifera, Lam) Menurunkan Ekspresi Caspase-3 Testis Tikus Wistar (Rattus norvegicus) Model Sindrom Metabolik Terinduksi Zhahira, Thania Nur; Budiani, Dyah Ratna; Setyawan, Novan Adi
Plexus Medical Journal Vol. 3 No. 2 (2024): April
Publisher : Fakultas Kedokteran, Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/plexus.v3i2.229

Pendahuluan: Sindrom metabolik berpengaruh terhadap kesuburan pria karena dapat menyebabkan infertilitas. Infertilitas antara lain disebabkan oleh kematian berlebih sel – sel spermatogenik selama spermatogenesis. Caspase-3 memiliki peran penting sebagai eksekutor apoptosis. Kandungan ekstrak etanolik daun kelor diketahui dapat mencegah apoptosis dengan menurunkan ekspresi caspase-3. Penelitian ini dilakukan dengan tujuan mengetahui pengaruh peningkatan dosis ekstrak etanolik daun kelor terhadap tingkat ekspresi caspase-3 pada jaringan testis tikus Wistar model sindrom metabolik. Metode: Metode penelitian yang digunakan adalah eksperimental laboratorik. Tikus Wistar berjumlah 30 ekor dibagi menjadi 5 kelompok dengan jumlah yang sama. KI sebagai kelompok kontrol negatif, KII sebagai kelompok sindrom metabolik terinduksi, KIII, KIV, dan KV sebagai kelompok sindrom metabolik terinduksi yang diberi dosis ekstrak etanolik daun kelor secara berurutan sebesar 150 mg/kgBB, 250 mg/kgBB, dan 350 mg/kgBB. Ekspresi caspase-3 dihitung menggunakan metode semikuantitatif IDS. Selanjutnya, ekspresi caspase-3 dianalisis menggunakan uji One-Way ANOVA, Post Hoc Tukey HSD, dan uji regresi linier. Hasil: KII memiliki skor ekspresi caspase-3 tertinggi yaitu 89,45 dan KV memiliki skor ekspresi caspase-3 terendah yaitu 25,93. Hasil uji One-Way ANOVA ekspresi caspase-3 pada jaringan testis tikus Wistar adalah 0.00 (p<0.05) yang artinya terdapat perbedaan signifikan ekspresi caspase-3 pada semua kelompok. Perbedaan bermakna terdapat antara KI dengan KII dan KII dengan KIII, KIV, dan KV. Hasil uji regresi linier menunjukkan pengaruh negatif antara dosis ekstrak etanolik daun kelor terhadap ekspresi caspase-3. Kesimpulan: Pemberian dosis ekstrak etanolik daun kelor yaitu 150 mg/kgBB, 250 mg/kgBB, dan 350 mg/kgBB dapat menurunkan ekspresi caspase-3.

Effect of Ethanolic Extract in Moringa oleifera, Lam. Leaf on Uric Acid Levels and Body Surface Area of Kidney Glomerulus in Wistar Rats (Rattus novergicus) Induced Metabolic Syndrome Model Darmawan, Muhammad Dzaki; Subandono, Jarot; Setyawan, Novan Adi; Budiani, Dyah Ratna
Indonesian Journal of Medicine Vol. 6 No. 4 (2021)
Publisher : Masters Program in Public Health, Sebelas Maret University

Background: There has been an increasing prevalence of metabolic syndrome (MS) caused by life style such as sedentary behavior and western diet. Metabolic syndrome causes degeneration in ren’s structure and processes the elimination of by product in metabolism, which is uric acid. Moringa oleifera Lam. leaves contain antioxidant that can repair damages caused by MS. Studies about improvement of ren’s structure and uric acid level related to Moringa oleifera Lam. leaves’ consumption has not been found yet. Therefore, this study was intended to examine the effects of ethanolic extract in Moringa oleifera Lam. leaves to uric acid and glomerular surface area in male wistar rat (Rattus norvegicus) with induced metabolic syndrome model.Method: This study is an experimental laboratory study. The subjects of this study consisted of 30 rats which were divided into 5 groups with 6 in each group. K1 is control group, K2 is MS group, and K3, K4, and K5 are MS groups given variety of ethanolic extract doses. The induction of MS was done by giving high-fat diet in 28 days and injection of streptozotocin-nicotinamide (STZ-NA) in the 25th day. Rats in group K3, K4, and K5 were given doses of 150, 250, and 350 mg/kgBW in 28 days.Results: The administration of high-fat diet for 28 days and injection of STZ-NA caused MS condition in rats. Repeated ANOVA and One-Way Anova test showed that the administration of ethanolic extract in Moringa oleifera Lam. leaves with doses of 150, 250, and 350 mg/kgBW in 28 days decreased uric acid significantly (p= 0.001; p=0.001; p= 0.001). Another result also found that ethanolic extract from Moringa oleifera Lam. leaves with doses of 250 and 350 mg/kgBW increased area of glomerular surface area in rats significantly.Conclusion: The administration of ethanolic extract from Moringa oleifera Lam. leaves with doses of 150, 250, and 350 mg/kgBW for 28 days decreased uric acid level in rats. Ethanolic extract of Moringa oleifera Lam. leaves 250 and 350 mg/kgBw doses increase glomerular surface area.Keywords: Moringa Oleifera, uric acid, glomerular cross-sectional area, metabolic syndrome, kidneyCorrespondence: Muhammad Dzaki Darmawan. Study Program of Medical Doctor Education, Faculty of Medicine, Universitas Sebelas Maret. Email: mdddzaki@student.uns.ac.id.Indonesian Journal of Medicine (2021), 06(04): 412-422https://doi.org/10.26911/theijmed.2021.06.04.07

Effect of Ethanolic Extract of Moringa oleifera, Lam. Roots on Expression of Cyclin D1 in Testicular Tissue of Metabolic Syndrome Induced Wistar Rats Lutfi, Sephendra; Budiani, Dyah Ratna; Setyawan, Novan Adi
Indonesian Journal of Medicine Vol. 8 No. 2 (2023)
Publisher : Masters Program in Public Health, Sebelas Maret University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26911/theijmed.2023.8.2.562

Background: Metabolic syndrome (MS) impacts on inhibition process of spermatogenesis by damaging various components of cell cycle proteins. Cyclin D1 protein which plays role in regulating the cell cycle of spermatogenic cells is depleted due to the metabolic syndrome. Variation results of M. oleifera studies on fertility are interesting for further research. This study aimed to determine the effect of ethanolic extract of M. oleifera roots towards expression of testicular cyclin D1 of metabolic syndrome induced Wistar rats.Subjects and Method: This study was an experimental laboratory. Thirty subjects of male Wistar rats were randomly divided into 5 groups consisted of 6 rats, control group (K1), MS group (K2), and treatment groups (K3, K4, and K5) which were given doses of 150, 250, and 350 mg/kgBW, respectively for 28 days. Induction of MS was achieved by high-fat diet for 28 days and injection of streptozotocin-nicotinamide (STZ-NA) on day 25. Cyclin D1 data was calculated using Intensity Distribution Score (IDS) and analyzed using one-way ANOVA test followed by post hoc multiple comparisons with Tukey HSD. Simple liniar regression test conducted on cyclin D1 data of K2, K3, K4, and K5.Results: One-way ANOVA test showed that the administration of ethanolic extract M. oleifera Lam. roots with doses of 150, 250, and 350 mg/kgBW in 28 days increased cyclin D1 significantly (p= 0.000). There were significant differences between K1 and K2 (p= 0.000), K2 and K3 (p= 0.000), K2 and K4 (p= 0.000), K2 and K5 (P= 0.000). Liniar regression test showed significant (p= 0.000) and moderate effect (R2= 0.65) of doses of ethanolic extract of M. oleifera roots on the expression of cyclin D1.Conclusion: The administration of ethanolic extract Moringa oleifera Lam. roots with doses of 150, 250, and 350 mg/kgBW for 28 days increased cyclin D1 in testicular rats.

Topical Application Type of Fish Oil Promotes Re-Epithelialization in Burn Wound Healing in Rats Sasongko, Heru; Pitaloka, Nirwana Asri Diah; Kurniawan, Muhammad Rizki; Pramono, Edi; Setyawan, Novan Adi; Rohman, Abdul
Majalah Obat Tradisional Vol 30, No 2 (2025)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.95644

Burns are a condition that is often found in the world in various cases such as accidents. The incidence of burns will probably increase as human activities become more complex. Wounds that are not treated immediately tend to become infected and cause death. Fish oil is one of the ingredients that has been studied for wound healing. Previous research shows that fish oil is rich in long-chain omega-3 fatty acids such as docosahexaenoic acid and eicosapentaenoic acid, which have anti-inflammatory effects. However, there has not been much research on the type of fish oil used and the wound healing process. The aim of this study was to determine the wound-healing activity of milkfish (Chanos chanos Forsskal.), patin (Pangasius djambal), and eel (Anguilla bicolor) fish oil. Tests were carried out on 25 mice as an experimental animal model for burn wounds. Data on percent wound reduction and the re-epithelialization process were statistically calculated using one-way analysis of variance (ANOVA) followed by a post-hoc LSD test. The research results showed that milkfish oil, patin fish oil, and eel fish oil in a concentration of 10% on an ointment basis were able to reduce wounds significantly. Eel fish oil shows the greatest burn wound healing process with re-epithelialization when compared to other oils. The type of fish oil affects the wound healing process.

Retinoblastoma tumor suppressor protein is not a risk factor for the location of condyloma acuminatum among adults Primisawitri, Pratiwi Prasetya; Mulianto, ⁠Nurrachmat; Ellistasari, Endra Yustin; Poncorini, Eti; Kariosentono, ⁠⁠Harijono; Widhiati, Suci; Wasita, Brian; Setyawan, Novan Adi; Yudhistira, Muhammad Yurizar
Universa Medicina Vol. 44 No. 3 (2025)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2025.v44.343-349

BackgroundCondylomata acuminata (CA) are sexually transmitted infections primarily caused by low-risk HPV types 6 and 11. Retinoblastoma protein (pRb) interacts with HPV oncoproteins, leading to destabilization, genomic instability, and carcinogenesis. This study aimed to determine whether variations in pRb expression and sexual orientation are risk factors of the anatomical location of CA lesions and to compare differences in pRb expression by sexual orientation. MethodsA cross-sectional study was conducted among 33 patients aged >18 years with clinically and histopathologically confirmed CA. Demographic data, pRb expression assessed via excision biopsy and immunohistochemistry, and relevant risk factors were collected. Statistical analyses including Simple and multiple binary logistic regression were used to analyze the data, with model fit assessed using the Hosmer–Lemeshow test. ResultsWeak pRb expression predominated in anal lesions (92.3%; p=0.018) and among homosexual/bisexual individuals (57.9%; p=0.031), while moderate expression was more common in genital lesions (66.7%; p=0.018) and heterosexual individuals (57.1%; p=0.011). Simple logistic regression analysis showed that HIV status (OR=11.88; p=0.025), sexual orientation (OR=13.33; p=0.001), and moderate-to-strong pRb expression (OR=24.00; p=0.008; OR=12.00; p=0.048) were associated with lesion location. In the multivariate model, pRb expression was excluded due to multicollinearity. Sexual orientation remained the only independent predictor of lesion location (Adj OR=8.89; p=0.023), with heterosexual individuals more likely to present with genital lesions. ConclusionThe expression of pRb was not a risk factor of the anatomical location of CA, and differed between genital and anal CA. However, sexual orientation emerged as the dominant independent factor associated with lesion location.

Effect of Ethanolic Extract of Moringa (Moringa oleifera, Lam.) Leaf on Seminiferous Tubules of Wistar Rats (Rattus norvegicus) Model of Metabolic Syndrome Devitasari, Sade; Pesik, Riza Novierta; Subandono, Jarot; Setyawan, Novan Adi
Smart Medical Journal Vol 6, No 2 (2023): August
Publisher : Faculty of Medicine Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/smj.v6i2.69345

Introduction: Metabolic syndrome define as symptoms of body abnormalities that increased reactive oxygen species and cause disturbances of the germ cell layer thickness in seminiferous tubules. The content of Moringa oleifera has anti-diabetic, anti-dyslipidemia, and hypotensive effects. This study aimed to find out the effect of ethanolic extract of moringa leaves (Moringa oleifera, Lam.) on the histopathology of seminiferous tubules of Wistar rats (Rattus norvegicus) induced metabolic syndrome with Streptozotocin-Nicotinamide and high-fat diet.Methods: This research was experimental post-only control group design. The subjects were 30 male Rattus norvegicus grouped into normal group (K1), metabolic syndrome group (K2), and 3 metabolic syndrome groups with ethanolic extract of moringa leaf at doses of 150 mg/kg (K3), 250 mg/kg (K4) and 350 mg/kg of body weight (K5).Results: Thickness of tubules (µm) of group K1 (107.48±10.74), K2 (53.87±25.09), K3 (80.52±3.50), K4 (81.94±9.40), K5 (94.04±8.66). Average number of cell layers of group K1 (8.00±1.09), K2 (3.65±1.35), K3 (5.99±0.36), K4 (6.57±0.36), K5 (6.72±0.20). There was a significant difference in tubule wall thickness and the number of cell layers between the five groups (p<0.05). The K3, K4, and K5 differed significantly from the K2 (p<0.05). K5 has value closest to K1. Conclusion: Ethanolic extract of moringa (Moringa oleifera, Lam.) leaf increased the wall thickness and number of cell layers of seminiferous tubules of Wistar rats (Rattus norvegicus) model of metabolic syndrome with ethanolic extract of moringa leaf at doses of 350 mg/kg of body weight most effectively improved the histopathological of the seminiferous tubules.

Effect of Moringa Leaves Ethanolic Extract (Moringa oleifera, Lam.) on Testicles Histopathological Structure in Metabolic Syndrome Rat Model Aji, Febriagi Bayu; Setyawan, Novan Adi; Hakim, Fikar Arsyad; Pesik, Riza Novierta
Smart Medical Journal Vol 6, No 1 (2023): April
Publisher : Faculty of Medicine Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/smj.v6i1.69243

Introduction: Metabolic syndrome was a collection of various disorders that can significantly increase the risk of experiencing insulin resistance, atherosclerotic cardiovascular disease, vascular complications, and diabetes mellitus. The detrimental effect of this condition has led to the development of various treatments options, such as Moringa leaves extract (Moringa oleifera), which was suspected to have ameliorative effects. Therefore, this study aimed to determine the effect of Moringa leaves extract on the histopathological appearance of the testes of Wistar rats induced with metabolic syndrome.Methods: This was an experimental design study, with a post-test-only control group, involving 30 Wistar rats (Rattus norvegicus). Metabolic syndrome was induced in the samples through the administration of high-fat and fructose feeds along with streptozotocin and nicotinamide. Furthermore, the moringa leaves extract was administered to the treatment groups in different doses of 150, 250, and 350 mg/kg BW. Testicular histopathological damage scores were then assessed on each group. The Kruskal-Wallis test which followed by Mann-Whitney test was used to determine the significant differences between the groups, while the correlation was analyzed using Spearman test.Results: There were significant differences in the histopathological scores between the treatment and control groups. Furthermore, the Wistar rats treated with Moringa extract showed better histopathological scores (2.1±0.64) compared to those without the treatment (2.7±0.54), p<0.05. The results also showed that higher doses of the extract significantly correlated with better outcome in testicular histopathological structure of metabolic syndrome rat model (p=0.000, r=-0.396).Conclusion: Moringa leaves ethanolic extract treatment improved testicular histopathological appearance in Wistar rats induced with metabolic syndrome.

Porang (Amorphophallus muelleri) Biscuits Fortified with Moringa (Moringa oleifera, Lamk) Leaf Flour on decrease Inflammation in kidney of Obese Wistar Rats (Rattus norvegicus) Safitri, Nisrina Ulayya; Setyawan, Novan Adi; Pesik, Riza Novierta; Budiani, Dyah Ratna
Smart Medical Journal Vol 7, No 2 (2024): August
Publisher : Faculty of Medicine Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/smj.v7i2.81652

Introduction: The high incidence of obesity is due to the habit of eating snacks. The habit of eating snacks can be replaced with healthy snacks, one of which is porang biscuits fortified with moringa flour. This study was conducted to determine the effect of intake of porang biscuits fortified with moringa flour on glomerular area and interstitial inflammation of the kidneys of Wistar rats modeled obesity.Methods: Laboratory experimental research with randomized postest control group design. 30 Wistar male rats were divided into 5 groups. The negative control group (KN) was given standard feed, the positive control group (KP) was given high-fat high-fructose feed, the treatment groups (P1, P2, and P3) were given porang biscuits fortified with moringa flour with varying doses of moringa leaf powder 150, 250, and 350 mg/200grBB after induction. Glomerular area was measured with raster image. The results of glomerular area and interstitial inflammation score were analyzed by oneway ANOVA and post hoc Tukey HSD. Correlation of glomerular area and interstitial inflammation score with increasing dose were analyzed by Pearson.Results: There was no significant difference in glomerular area between all groups and no correlation between glomerular area and increasing dose of moringa powder. There were significant differences in interstitial inflammation scores between KN-KP, KN-P1, KN-P2, KN-P3, KP-P3, P1-P3 and P2-KP. The correlation between interstitial inflammation and increasing dose of moringa leaf powder was p=0.000 and r=-0.776Conclusion: There is a difference in the interstitial inflammation score of the kidneys of obese Wistar rats after intake of porang biscuits fortified with Moringa leaf powder at a dose of 350 mg/200 grBB while there is no difference in glomerular area. There is a strong negative correlation between increasing doses of moringa leaf powder and interstitial inflammation.

Pengaruh Ekstrak Etanolik Daun Kelor (Moringa oleifera, Lam.) Terhadap Ekspresi NF-κβ Pankreas Tikus Wistar (Rattus norvegicus) Model Sindroma Metabolik Terinduksi Naufal, Naila Rahima; Budiani, Dyah Ratna; Setyawan, Novan Adi
Plexus Medical Journal Vol. 2 No. 5 (2023): Oktober
Publisher : Fakultas Kedokteran, Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/plexus.v2i5.629

Pendahuluan: Sindrom metabolik (SM) menyebabkan kerusakan pulau langerhans pankreas akibat aktivasi NF-κβ. Kandungan fitokimia dari ekstrak etanolik daun kelor mencegah kerusakan pulau langerhans pankreas. Penelitian ini bertujuan untuk mempelajari pengaruh peningkatan dosis ekstrak etanolik daun kelor terhadap ekspresi NF-κβ pankreas tikus wistar model SM terinduksi. Metode: Penelitian ini bersifat eksperimental laboratorik. Subjek penelitian berjumlah 30 ekor tikus wistar dibagi menjadi 5 kelompok yaitu KI kelompok kontrol normal, KII kelompok kontrol positif, dan kelompok perlakuan SM dengan dosis ekstrak etanolik daun kelor 150mg/kgBB/hari pada KIII, 250mg/kgBB/hari pada KIV, dan 250mg/kgBB/hari pada KV. Model tikus SM diberikan diet tinggi lemak dan STZ-NA. Preparat IHC diamati dengan mikroskop cahaya dan image raster kemudian dihitung skor IDS. Analisis data menggunakan uji Kruskal Wallis dilanjutkan dengan uji post-hoc tes Mann-Whitney U (p<0,05). Hasil: Rerata IDS tertinggi pada KII dan terendah pada KI. Hasil uji Kruskal Wallis menunjukan adanya pengaruh ekstrak etanolik daun kelor terhadap ekspresi NF-κβ (p=0,035). Uji post-hoc menunjukan hasil yang signifikan pada KII dengan KI (p=0,01) dan KIII (p=0,037). Tidak terdapat perbedaan yang signifkan antar kelompok perlakuan. Kesimpulan: Ekstrak etanolik daun kelor dosis150 mg/kgBB/hari menurunkan ekspresi NF-κβ pankreas tikus wistar (Rattus norvegicus) model sindrom metabolik terinduksi, namun peningkatan dosis ekstrak etanolik daun kelor (Moringa oleifera, Lam.) tidak menurunkan ekspresi NF-κβ pankreas tikus wistar (Rattus norvegicus) model sindrom metabolik terinduksi.

Peran Ekstrak Etanolik Daun Kelor (Moringa oleifera, Lam.) Terhadap Peningkatan Ekspresi GLUT-2 Pankreas Tikus Wistar (Rattus Norvegicus) Jantan Model Sindrom Metabolik Terinduksi Nur Illahi, Fadhila; Setyawan, Novan Adi; Pesik, Riza Novierta; Suparyanti , Endang Listyaningsih
Plexus Medical Journal Vol. 2 No. 5 (2023): Oktober
Publisher : Fakultas Kedokteran, Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/plexus.v2i5.877

Pendahuluan: Konsumsi tinggi lemak dan tinggi gula tanpa diimbangi dengan olahraga rutin mampu meningkatkan risiko sindrom metabolik yang mampu mendestruksi sel β pankreas yang mengakibatkan GLUT-2 berkurang jumlahnya. Antioksidan pada daun kelor mampu memperbaiki kerusakan tersebut. Penelitian ini terkait ekspresi GLUT-2 pankreas dengan pemberian daun kelor yang masih belum ditemukan. Sehingga, penelitian ini bertujuan untuk mengetahui perbedaan ekspresi GLUT-2 pankreas tikus Galur Wistar (Rattus norvegicus) jantan model sindrom metabolik dengan pemberian ekstrak etanolik. Metode: Penelitian eksperimental laboratorik. 30 ekor tikus wistar dibagi menjadi 5 kelompok: KKN merupakan kontrol normal; KK- merupakan kontrol negatif induksi sindrom metabolik saja ; KP1, KP2, KP3 induksi sindrom metabolik dan pemberian ekstrak etanolik daun kelor selama 28 hari dengan dosis secara berurutan sebesar 150 mg/KgBB, 250 mg/KgBB, dan 350 mg/KgBB. Induksi sindrom metabolik dilakukan dengan pakan tinggi lemak selama 28 hari dan induksi STZ-NA di hari ke-25. Ekspresi GLUT-2 diamati dan dihitung menggunakan rumus IDS dan uji One-Way ANOVA dilanjutkan uji post-hocTuckey HSD, serta uji regresi linier. Hasil: Uji One-Way ANOVA didapatkan perbedaan bermakna p=0,000 (p<0,05) pada tiap kelompok. Uji post-hoc Tuckey HSD didapatkan perbedaan bermakna (p<0,05) antar kelompok KKN dan KK- serta KK- dengan KP1, KP2, KP3. Uji regresi linier menunjukkan pengaruh dosis ekstrak etanolik daun kelor memiliki arah positif. Kesimpulan: Ekstrak etanolik daun kelor meningkatan ekpresi GLUT-2 jaringan pankreas tikus Wistar model sindrom metabolik.

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