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All Journal Vegetalika Jurnal Ilmu Pertanian International Journal of Biosciences and Biotechnology Jurnal Teknosains Indonesian Journal of Biotechnology Indonesian Journal of Chemistry jurnal Ilmu Pertanian (Agricultural Science)

Sri Wedhastri

Department Of Agricultural Microbiology, Faculty Of Agriculture, Universitas Gadjah Mada, Yogyakarta

Author-ID : 413719

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mechanical Engineering

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Articles

Legume Nodulating Bacterium, Achromobacter xylosoxidans Found in Tropical Shrub Agroecosystem, Sumatera, Indonesia Wedhastri, Sri; Fardhani, Dinar Mindrati; Kabirun, Siti; Widada, Jaka; Widianto, Donny; Evizal, Rusdi; Prijambada, Irfan Dwidya
Indonesian Journal of Biotechnology Vol 18, No 2 (2013)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (183.716 KB)

Legume nodulating bacteria (LNB), known also as rhizobia, are soil bacteria, which are able to form rootnodules and fi x nitrogen in the leguminous plants. The LNB availability in the soil depends on the type ofagroecosystem, where plant grows. In this study, we isolated LNB from the shrub agroecosystem in Sumatera,Indonesia, and obtained four selected bacterial strains. Among them, the isolate UGM48a formed root nodulein Macroptilium atropurpureum and showed highest number of nitrogenase activity. UGM48a also contains nifHand nodA genes. An analysis of the PCR-amplifi ed 16S rDNA and BLASTn analysis showed that UGM48adisplayed 96% similarity with Achromobacter xylosoxidans. In addition, UGM48a were successfully nodulatedGlycine max (L.) merr var. wilis. This is the fi rst report detecting A. xylosoxidans as nodule-forming species forGlycine max possesing the positive copy of nodA gene.Keywords : Legume Nodulating Bacteria, shrub agroecosystem, Achromobacter xylosoxidans, nodA, Glycine max

The Diversity of Legume-Nodulating Bacteria from Several Agroecosystems in Sumberjaya, Lampung Wedhastri, Sri; Yuliana Prahastiwi, Yuliana; Widada, Jaka; Widianto, Donny; Kabirun, Siti
Indonesian Journal of Biotechnology Vol 18, No 1 (2013)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (257.082 KB)

Bacteria that capable of forming root nodules on legumes are known as Rhizobia. They have also known as Legume- Nodulating Bacteria (LNB). They can fi x nitrogen from the atmosphere. Diversity of Legume-Nodulating Bacteria is affected by biotic factors (such as their genetic factors, plants, and competition with the other soil microbes) and abiotic factors (such as land use, soil’s temperature, pH, chemistry and soil’s properties). The aim of this experiment is to know the diversity of eleven Legume- Nodulating Bacteria based on their phenotypic and genotypic characters. The eleven LNB used in this experiments were isolated from several agroecosystems in Sumberjaya, Lampung. The analysis of these LNB diversity were carried out by characterizing both phenotypic and genotypic properties. The diversity analysis showed that the eleven LNB isolates had high diversity, based on nodule formation, and classifi ed into two groups of cross inoculation group.Key words: Rhizobia, phenotypic diversity, genotypic diversity

The Diversity of Legume-Nodulating Bacteria from Several Agroecosystems in Sumberjaya, Lampung Sri Wedhastri; Yuliana Prahastiwi; Jaka Widada; Donny Widianto; Siti Kabirun
Indonesian Journal of Biotechnology Vol 18, No 1 (2013)
Publisher : Universitas Gadjah Mada

Bacteria that capable of forming root nodules on legumes are known as Rhizobia. They have also known as Legume- Nodulating Bacteria (LNB). They can fi x nitrogen from the atmosphere. Diversity of Legume-Nodulating Bacteria is affected by biotic factors (such as their genetic factors, plants, and competition with the other soil microbes) and abiotic factors (such as land use, soil’s temperature, pH, chemistry and soil’s properties). The aim of this experiment is to know the diversity of eleven Legume- Nodulating Bacteria based on their phenotypic and genotypic characters. The eleven LNB used in this experiments were isolated from several agroecosystems in Sumberjaya, Lampung. The analysis of these LNB diversity were carried out by characterizing both phenotypic and genotypic properties. The diversity analysis showed that the eleven LNB isolates had high diversity, based on nodule formation, and classifi ed into two groups of cross inoculation group. Key words: Rhizobia, phenotypic diversity, genotypic diversity

Legume Nodulating Bacterium, Achromobacter xylosoxidans Found in Tropical Shrub Agroecosystem, Sumatera, Indonesia Sri Wedhastri; Dinar Mindrati Fardhani; Siti Kabirun; Jaka Widada; Donny Widianto; Rusdi Evizal; Irfan Dwidya Prijambada
Indonesian Journal of Biotechnology Vol 18, No 2 (2013)
Publisher : Universitas Gadjah Mada

Legume nodulating bacteria (LNB), known also as rhizobia, are soil bacteria, which are able to form rootnodules and fi x nitrogen in the leguminous plants. The LNB availability in the soil depends on the type ofagroecosystem, where plant grows. In this study, we isolated LNB from the shrub agroecosystem in Sumatera,Indonesia, and obtained four selected bacterial strains. Among them, the isolate UGM48a formed root nodulein Macroptilium atropurpureum and showed highest number of nitrogenase activity. UGM48a also contains nifHand nodA genes. An analysis of the PCR-amplifi ed 16S rDNA and BLASTn analysis showed that UGM48adisplayed 96% similarity with Achromobacter xylosoxidans. In addition, UGM48a were successfully nodulatedGlycine max (L.) merr var. wilis. This is the fi rst report detecting A. xylosoxidans as nodule-forming species forGlycine max possesing the positive copy of nodA gene. Keywords : Legume Nodulating Bacteria, shrub agroecosystem, Achromobacter xylosoxidans, nodA, Glycine max

Pengujian Lima Pupuk Organik Cair Komersial dan Pupuk NPK pada Jagung (Zea mays L.) Arif Meftah Hidayat; Erlina Ambarwati; Sri Wedhastri; Panjisakti Basunanda
Vegetalika Vol 4, No 4 (2015)
Publisher : Universitas Gadjah Mada

Berbagai produk pupuk organik cair yang dipasarkan memiliki kandungan bahan yang berbeda yang perlu diuji efektivitasnya. Uji efektivitas dilakukan untuk mendapatkan macam pupuk organik cair yang sesuai untuk jagung serta mendapatkan kombinasi takaran pupuk anorganik yang paling sesuai dengan berbagai produk pupuk organik cair untuk mendukung pertumbuhan dan hasil jagung. Penelitian dilaksanakan pada bulan Mei-September 2014 di Kebun Pendidikan, Penelitian, dan Pengembangan Pertanian (KP4) UGM. Penelitian menggunakan rancangan acak kelompok lengkap untuk menguji macam pupuk organik cair yaitu BMN, BMF, NS, BN, dan BST serta takaran pupuk anorganik yang terdiri atas 0%, 35%, dan 75% dari rekomendasi dinas pertanian. Kontrol merupakan takaran pupuk anorganik 100%, yaitu urea 300 kg/ha, SP-36 100 kg/ha, dan KCl 75 kg/ha. Data pertumbuhan dan komponen hasil dianalisis varian menurut kaidah rancangan acak kelompok lengkap dilanjutkan dengan uji HSD dengan taraf kepercayaan masing-masing 95%. Hasil penelitian menunjukkan bahwa macam pupuk organik cair memberikan pengaruh yang sama terhadap pertumbuhan dan hasil jagung tetapi tidak dapat meningkatkan pertumbuahan dan hasil jagung. Takaran pupuk anorganik 75% dan 100% rekomendasi nyata meningkatkan pertumbuhan tanaman sebesar 25% dan meningkatkan produktivitas sebesar 50% jika dibandingkan tanpa pupuk anorganik. Pupuk organik cair dapat menghemat penggunaan pupuk anorganik sebesar 25% terhadap pertumbuhan dan hasil jagung (Zea mays L.).

Penurunan Kadar Glukosida Sianogenik Biji Koro Benguk (Mucuna Pruriens, d.c.) oleh Aktivitas Fermentasi Aspergillus Oryzae, A. Sojae, Rhizopus Oligosporus dan R. Oryzae. Sri Wedhastri
Jurnal Ilmu Pertanian Vol 5, No 2 (1992): September
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.58671

PHYTOREMEDIATION OF MERCURY CONTAMINATED SOILS IN A SMALL SCALE ARTISANAL GOLD MINING REGION OF INDONESIA Kokyo Oh; Sachiko Takahi; Sri Wedhastri; Hardita Librasanti Sudarmawan; Retno Rosariastuti; Irfan Dwidya Prijambada
International Journal of Biosciences and Biotechnology Vol 3 No 1 (2015)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (336.722 KB)

In the small gold mining regions of Indonesia, the tailings or waste water containing mercury commonly may be released into agricultural lands resultimg soil contamination. Phytoremediation is a low-cost and environmental friendly alternative to traditional techniques such as soil heating, soil removal, and soil washing. In this study, a sweet sorghum combined with the inoculation of a rhizobacteria, Agrobacterium tumefaciens, was tested in a field experiment with mercury contaminated soil from a small scale gold mining. Plant growth, uptake and accumulation of mercury by the sweet sorghum, and effects of the Agrobacterium tumefaciens inoculation on mercury accumulation were investigated. The average of mercury content in the soil was 3.76 mg/kg. The results showed that the sorghum grew well, and the inoculation of Agrobacterium tumefaciens promoted the plant growth, but did not increased the mercury concentration in both root and stem parts of the sorghum. The accumulation of mercury was 6.2?/plant for sorghum without Agrobacterium tumefaciens, and 14.0?/plant for sorghum with Agrobacterium tumefaciens. It was estimated that the phytoremediation efficiency of mercury was 414 and 934 mg/ha for sweet sorghum without Agrobacterium tumefaciens inoculation and with Agrobacterium tumefaciens inoculation, respectively.

PERBAIKAN PROSES FERMENTASI BIJI KAKAO KERING DENGAN PENAMBAHAN TETES TEBU, KHAMIR, DAN BAKTERI ASAM ASETAT Donny Widianto, Ajeng Dara Pramita, dan Sri Wedhastri
Jurnal Teknosains Vol 3, No 1 (2013): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/teknosains.6126

Most of cocoa beans produced by smallholder farmers were non fermented which can be improved by modifiedfermentation processing. This study was aimed to inverstigate the influence of molasses, yeast Saccharomycescerevisiae, and Acetobacter aceti addition on dried cocoa beans fermentation process.Fresh cocoa beans were dried in a glasshouse and its reducing sugar was analyzed before and after drying. Asmall plastic bucket (20 cm diameter and 30 cm height) with aeration holes was used as fermentation vessel. Driedcocoa beans were soaked in distilled water for 4 hours, inoculated with yeast and acetic acid bacteria cultures, andmolasses were added at two different concentration, i.e, 1 and 1.5 times of reducing sugar lost during drying.Reducing sugar, ethanol, titrated acid, population of yeast, and acetic acid bacteria were monitored duringfermentation. After fermentation the beans were sun dried and its pH and degree of fermentation were determinedto assess the bean quality.The results showed that the addition of molasses mostly at the level of 1.5, S. cerevisiae, and A. aceti increasereducing sugar, ethanol, titrated acid, yeast and acetic acid bacteria of fermentation liquid (pulp). The highestpercentage of fermented beans (68.4 %) was achieved by addition of S. cerevisiae, A. aceti, and molasses atthe level 1.5. It is likely that the addition of S. cerevisiae, A. aceti, and molasses could improve fermentationprocessing of dried cocoa bean.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.58671

ADSORPTION OF AFLATOXIN B1 IN CORN ON NATURAL ZEOLITE AND BENTONITE Nuryono Nuryono; Ali Agus; Sri Wedhastri; Y.M.S. Maryudhani; Deni Pranowo; Yunianto Yunianto; Ebrahim Razzazi-Fazeli
Indonesian Journal of Chemistry Vol 12, No 3 (2012)
Publisher : Universitas Gadjah Mada

A study on adsorption of AFB1 in corn (kernel and grained) on natural zeolite and bentonite has been investigated. The first work was adsorption in a batch system of standard AFB1 solution on adsorbents. Some factors such as contact time, concentration of AFB1 and particle size of adsorbent were evaluated. The amount of AFB1 adsorbed was calculated based on the difference of AFB1 concentration before and after adsorption determined by high performance liquid chromatography (HPLC) method. Adsorption of AFB1 in corn sample was emphasized by mixing aqueous suspension of sample with adsorbent. Concentration of AFB1 in suspension was analyzed by enzyme-linked immuno-sorbent assay (ELISA) method. Result shows that adsorption of AFB1 on adsorbents of natural zeolite and bentonite is very fast. Within 15 min 99% of AFB1 (200 ng/mL) has been adsorbed by 25 mg of bentonite and 96% by zeolite. The particle size higher than 200 mesh did not give significant effect on the AFB1 adsorption capability. Effectiveness of zeolite in adsorbing AFB1 is lower than that of bentonite. Capability in reducing AFB1 contamination in corn samples (kernel and meal) for both adsorbents is lower than that in standard solution.

Co-Authors Ali Agus Arif Meftah Hidayat Deni Pranowo Dinar Mindrati Fardhani Donny Widianto Donny Widianto Donny Widianto Ebrahim Razzazi-Fazeli Erlina Ambarwati Hardita Librasanti Sudarmawan Irfan Dwidya Prijambada Irfan Dwidya Prijambada JAKA WIDADA Kokyo Oh Nuryono Nuryono Panjisakti Basunanda Retno Rosariastuti Rusdi Evizal Sachiko Takahi Siti Kabirun SITI KABIRUN Siti Kabirun Y.M.S. Maryudhani Yuliana Prahastiwi Yuliana Yuliana Prahastiwi, Yuliana Yunianta Yunianta

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