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Journal : Journal Health Applied Science And Technology (JHAST)

PERBEDAAN HITUNG JUMLAH LEUKOSIT SEGERA DAN DISIMPAN SELAMA 6 JAM Aristoteles; Nanik Puspitasari
jurnal kesehatan terapan sains dan teknologi Vol 1 No 1 (2023): Journal Health Applied Science And Technology (JHAST)
Publisher : IKesT Muhammadiyah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52523/jhast.v1i1.4

Abstract

Introduction: Leukocytes are blood cellscontaining nuclei, orso-called white blood cells. Leukocytes have a role in the cellular and humoral defense of organisms against foreign substances. Some types of leukocytes or white blood cells are present in the blood. Leukocytes are generally divided into granuocytes (which have special granules) andagranulocytes (which do not have special granules). Granulocytes consist of neutrophils, eosinophils, and basophils. Agranulocytes consist of lymphocytes and monocytes. Leukocyte count examination is a routine blood test conducted in the clinical laboratory because of the frequent request for a test to calculate the number of leukocytes. Calculate the number of leukocytes not only determinethe number ofleukocyte cells, but can also be any type of leukocyte cells. Objectives: The purpose of this study is to determine the difference in the number of leucocytes immediately and stored for 6 hours.Methods: This research method usescross sectional, this sample was taken from studentslevel 1 and 4Medical Laboratory Technologyof Health Sciences and Technology Muhammadiyah Palembang. Result: The results of this study obtained an averagecount of the number of leukocytes immediately is 6.3667 cells/μl, while the average count of the number of leukocytes stored for 6 hours is6.4667 cells/μl. Result: The results of T-test statistics showed a significant value (0,477) was higher than the alpha value of 0.05 or ρ>ɑthen Ho, receivedwhich means there is no difference in calculating the number of leukocytes immediately and stored for 6 hours. Conclusion: The conclusion of this study is that there is no difference in calculating the number of leukocytes immediately and disinced for 6 hours.
PERBANDINGAN JUMLAH LEUKOSIT YANG DIHITUNG MENGGUNAKAN MODIFIKASI AIR PERASAN BUAH JERUK KUNCI (Citrus microcarpa Bunge) DAN LARUTAN TURK Aristoteles; Denny Juraijin
jurnal kesehatan terapan sains dan teknologi Vol 1 No 2 (2023): Journal Health Applied Science And Technology (JHAST)
Publisher : IKesT Muhammadiyah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52523/jhast.v1i2.11

Abstract

Background : Laboratory tests, especially hematology, are frequently requested by doctors to help make a diagnosis. White blood cells (leukocytes) are an important part of the body’s defense system whose function is to fight infection causing microorganisms, tumor cells, and harmful foreign substances. Counting the number of leukocytes by manual method is done by diluting the blood using Tur ation, but the problem is that sometimes the Turk solution is not available. or is available but has expired, so an alternative is done by modifying key orange. juice (Citrus macrocarpa Bunge). Research purposes : To determine the difference in the number of leukocytes counted using modified key lime juice (Citrus microcarpa Bunge) and Turk solution. Research Methods : Experimental this research was conducted on January 19, 2023 at Bunda Hospital Palembang. and sampling was carried out at the IkeST Muhammadiyah Palembang Hematology Laboratory, totaling 31 EDTA blood samples. Results : The results obiained in this study showed that there was a difference in the leukocyte count with the modification of key orange juice (Citrus macrocarpa Bunge), turk solution, and a hematology analyzer. The results of the research data were carried out using data analysis with the SPSS program with the Shapiro–Wilk normality test and continued with the Kruskal test. Conclusion : There is a difference between the leukocyte count examination using modified key lime juice (Citrus macrocarpa Bunge) and Turk solution.
Perbedaan Kadar HbA1c Pada EDTA Yang Diperiksa Segera Dan Disimpan 2 Jam Disuhu Ruang (20-25℃): Perbedaan Kadar HbA1c Pada EDTA Yang Diperiksa Segera Dan Disimpan 2 Jam Disuhu Ruang (20-25℃) Aristoteles; Bastian
jurnal kesehatan terapan sains dan teknologi Vol 2 No 2 (2024): Journal Health Applied Science And Technology (JHAST)
Publisher : IKesT Muhammadiyah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52523/jhast.v2i2.51

Abstract

Latar belakang: Diabetes Melitus (DM) merupakan pembunuh nomor empat di dunia. Pemeriksaan HbA1c mencerminkan konsentrasi glukosa darah jangka Panjang. Penggunaan HbA1c sebagai Tindakan diagnostic merupakan standar perawatan oleh American Diabetes Association berdasarkan rekomendasi dari International Expert Committee. Stabilitas penundaan analisis menunjukkan bahwa inflasi nilai HbA1c berhubungan langsung dengan waktu penyimpanan, kondisi penyimpanan sampel harus dipantau dan dikontrol dengan hati-hati. Pemeriksaan HbA1c menggunakan metode Immunoassay dengan alat FinacareTM FIA Meter Plus Wondfo dari sampel pasien diabetes melitus sebanyak 29 sampel. Tujuan penelitian: Untuk mengetahui apakah ada perbandingan kadar HbA1c pada darah EDTA yang diperiksa segera dan yang disimpan 2 jam disuhu ruang 20-25°C. Metode penelitian: menggunakan studi Experimental Laboratory dengan rancangan one group pretest and posttest design. Data yang didapatkan diolah dengan program SPSS dilakukan uji Normalitas dengan uji Shapiro – wilk Bila hasil terdistribusi normal maka dilanjutkan uji hipotesis dengan uji – T berpasangan (Paired sampel T test). Dan jika tidak normal dilanjutkan dengan transformasi data, dan jika tidak normal maka dilakukan dengan uji non parametrik dengan Wilcoxon. Hasil: Hasil data yang dilakukan dengan uji (Paired Sampel T Test) didapatkan signifikan p = 0,959. Yaitu tidak ada perbedaan. Simpulan: Berdasarkan hasil penelitian dapat disimpulkan bahwa antara pemeriksaan kadar HbA1c pada EDTA yang diperiksa segera dan disimpan 2 jam disuhu ruang (20-25℃) tidak terdapat perbandingan yang signifikan, artinya pada penyimpanan 2 jam disuhu ruang (20-25℃) sampel untuk pemeriksaan HbA1c masih memiliki hasil yang baik.