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All Journal JAM : Jurnal Aplikasi Manajemen Jurnal Akuntabilitas Manajemen Pendidikan Jurnal Bioteknologi & Biosains Indonesia (JBBI) UNES Journal of Swara Justisia Pharmaceutical Sciences and Research (PSR) terAs Law Review: Jurnal Hukum Humaniter dan HAM Amicus Curiae Jurnal Bioteknologi & Biosains Indonesia (JBBI) Thalaqah : Journal of Arabic Education and Literature
Aji Wibowo
Universitas Bakrie
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Other

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Journal : Jurnal Bioteknologi

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TEMPERATURE EFFECTS ON THE STABILITY OF MYCOBACTERIUM TUBERCULO-SIS SHIKIMATE KINASE (MtSK): STRATEGIES FOR SECURE TRANSPORT Wibowo, Aji; Komariyah, Tinta; Prabandari, Erwahyuni Endang; Ariyani, Titin; Siska, Eka
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 1 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.5751

Abstract

Mycobacterium tuberculosis Shikimate Kinase (MtSK) has a crucial role in the shikimic pathway, which is essential for this bacteria but is absent in humans, making it a potential target for novel anti-tuberculosis drugs. This study used enzyme-coupled fluorescence to examine the stability of MtSK stored in 50% glycerol at -30 ℃, 4 ℃, and ±28 ℃ for six days. Results showed stable enzyme activity values (α=0.05) at all temperatures. This research underscores that MtSK’s stability depends on its molecular properties, including GC content, hydrophobic residues, Mg2+ binding, and intra-helical salt bridge. Despite some activity decline over time due to glycerol-induced aggregation, MtSK can be safely transported at ±28 ℃ for up to six days without special cooling compartment. Understanding MtSK stability ensures its active conformation remains consistent, reducing off-target effects on drug design and enhancing drug efficacy. This insight ultimately leads to high-quality and commercially viable tuberculosis treatment development. Future research should explore MtSK stability at higher temperatures and assess the optimal glycerol content for cryopreservation.
Co-Authors Ariyani, Titin Catur Sriherwanto, Catur Christin Susilowati Dzuhria, Ade Rizky Endang Sri Budi Herawati ERWAHYUNI ENDANG PRABANDARI Etyn Yunita Fajwa, R. Halya Zayna Firman, Hamdi Firdaus Gunawan, Suryanto Komariyah, Tinta Kusuma, Muhammad Aziz Eka Surya Marianti Avi Lathifah, Marianti Avi Maulana, Rafli Syah Much Ilham, Much Muniroh, Arina Nasikul Mustofa Efendi Otong Rosadi sagitarisa, heince Salsabilah, Nahdia Saputra, Henry Kasmanhadi Siska, Eka Suja'i, Imam Susanti Susanti Wiwik Wijayanti